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The map label for this gene is rimK [H]

Identifier: 126207968

GI number: 126207968

Start: 553298

End: 554212

Strand: Direct

Name: rimK [H]

Synonym: APL_0484

Alternate gene names: 126207968

Gene position: 553298-554212 (Clockwise)

Preceding gene: 126207967

Following gene: 126207970

Centisome position: 24.33

GC content: 45.68

Gene sequence:

>915_bases
ATGAAATTTTTAATGTTGTGTCGTGAGCCTCGTTTATACAGTTGCCAACGTTTACAACAGGCTTGTGAGCAACGGGGAAT
TAGCTTAGATATTCTCGACCCGAATCGAATGTTAATAAAATTAGCGGTGGAGCAAGGTCAATCCGTATTGCGGCTCTATT
ACCAAACAGGGGAAATTTACGATAAAAACCGACCGCTTCCGCAATTATTACCCGATTATGATGCGGTGTTGCCTCGTTTC
GGCACAACCAGTACGGAAATGGGATGTCGAGTTTTGCGTTATTTTGAAGCGAAACGGGTAAAAGTATTAAATGATTCGAC
CGCTTTCGCTTTGGCACGAGATAAATGGCTAAGTTTACAAGTTTTAGTTGCCGCACAAATTGCAGTGCCGGCAAGTTCGT
TAGCCGGAGAACTGTGTTCGATGGACGGACATTTAGCCCAACATCAGTTCCCGTTGATAATGAAAACCTTATCCGGTTCG
CAAGGTGTAGGGGTTATGTTAGCCGAGAGCAAACAAACCGCCCAAAGTGTCTTAGGGGTTTTACAGCAAGCGAAATTAAC
TTATTTGCTGCAAGATTTCGTAGCGAAGTCAAAAGGGCAGGATATTCGAGCGTTTGTCATCGGAGATCGTGTCGTGGCGG
CGATGCAACGTTCAAGTAATGAGGGCGAATTTCGTGCAAATATCCATTTAGGCGGTAAAGCACAACTGATAGCATTAACT
GAAGCGGAACAGCAGCTTGCGGTAAAAGCGGCAAACGCCATCGGACTTAGCGTGGCGGGTGTTGACCTGATTCGTTCCGA
TAGCGGATTGTTGGTATTGGAAGTAAATGCCAGCCCGGGGCTGGAAGGGATTGAACAGGCAAGCGGTATTGATATTGCCG
GTTTGATGATCGAACATCTGATTCATTCACTTTAG

Upstream 100 bases:

>100_bases
GTGAAGGACAGCAGTGGCAAGCGGCGATTGACAAAACCTTAAATAAAGCGGTGCGTCCGCATATGTTACCGTTTTTCCAA
CAACAAGGATTGTTGAAGCG

Downstream 100 bases:

>100_bases
TACAAAGCCCTCAAACGGGGCTTTATTATGCTAATTATCCGATCAGTTTTTCTTTCCAATAACCAATCCACTCTTTAAGT
GCAATGCTACTGTTCTGTAA

Product: ribosomal protein S6 modification protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKFLMLCREPRLYSCQRLQQACEQRGISLDILDPNRMLIKLAVEQGQSVLRLYYQTGEIYDKNRPLPQLLPDYDAVLPRF
GTTSTEMGCRVLRYFEAKRVKVLNDSTAFALARDKWLSLQVLVAAQIAVPASSLAGELCSMDGHLAQHQFPLIMKTLSGS
QGVGVMLAESKQTAQSVLGVLQQAKLTYLLQDFVAKSKGQDIRAFVIGDRVVAAMQRSSNEGEFRANIHLGGKAQLIALT
EAEQQLAVKAANAIGLSVAGVDLIRSDSGLLVLEVNASPGLEGIEQASGIDIAGLMIEHLIHSL

Sequences:

>Translated_304_residues
MKFLMLCREPRLYSCQRLQQACEQRGISLDILDPNRMLIKLAVEQGQSVLRLYYQTGEIYDKNRPLPQLLPDYDAVLPRF
GTTSTEMGCRVLRYFEAKRVKVLNDSTAFALARDKWLSLQVLVAAQIAVPASSLAGELCSMDGHLAQHQFPLIMKTLSGS
QGVGVMLAESKQTAQSVLGVLQQAKLTYLLQDFVAKSKGQDIRAFVIGDRVVAAMQRSSNEGEFRANIHLGGKAQLIALT
EAEQQLAVKAANAIGLSVAGVDLIRSDSGLLVLEVNASPGLEGIEQASGIDIAGLMIEHLIHSL
>Mature_304_residues
MKFLMLCREPRLYSCQRLQQACEQRGISLDILDPNRMLIKLAVEQGQSVLRLYYQTGEIYDKNRPLPQLLPDYDAVLPRF
GTTSTEMGCRVLRYFEAKRVKVLNDSTAFALARDKWLSLQVLVAAQIAVPASSLAGELCSMDGHLAQHQFPLIMKTLSGS
QGVGVMLAESKQTAQSVLGVLQQAKLTYLLQDFVAKSKGQDIRAFVIGDRVVAAMQRSSNEGEFRANIHLGGKAQLIALT
EAEQQLAVKAANAIGLSVAGVDLIRSDSGLLVLEVNASPGLEGIEQASGIDIAGLMIEHLIHSL

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=234, Percent_Identity=28.6324786324786, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI134254462, Length=269, Percent_Identity=28.6245353159851, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1787076, Length=306, Percent_Identity=41.8300653594771, Blast_Score=228, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 33149; Mature: 33149

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLMLCREPRLYSCQRLQQACEQRGISLDILDPNRMLIKLAVEQGQSVLRLYYQTGEIY
CCEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEHHHCHHEEHHHHHCCCEE
DKNRPLPQLLPDYDAVLPRFGTTSTEMGCRVLRYFEAKRVKVLNDSTAFALARDKWLSLQ
CCCCCCHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCEEE
VLVAAQIAVPASSLAGELCSMDGHLAQHQFPLIMKTLSGSQGVGVMLAESKQTAQSVLGV
EEEHHHHHCCHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCCCCEEEEECCHHHHHHHHHH
LQQAKLTYLLQDFVAKSKGQDIRAFVIGDRVVAAMQRSSNEGEFRANIHLGGKAQLIALT
HHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEEE
EAEQQLAVKAANAIGLSVAGVDLIRSDSGLLVLEVNASPGLEGIEQASGIDIAGLMIEHL
CHHHHHHHHHHHHHEEEEECEEEEECCCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHHH
IHSL
HHCC
>Mature Secondary Structure
MKFLMLCREPRLYSCQRLQQACEQRGISLDILDPNRMLIKLAVEQGQSVLRLYYQTGEIY
CCEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEHHHCHHEEHHHHHCCCEE
DKNRPLPQLLPDYDAVLPRFGTTSTEMGCRVLRYFEAKRVKVLNDSTAFALARDKWLSLQ
CCCCCCHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCEEE
VLVAAQIAVPASSLAGELCSMDGHLAQHQFPLIMKTLSGSQGVGVMLAESKQTAQSVLGV
EEEHHHHHCCHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCCCCEEEEECCHHHHHHHHHH
LQQAKLTYLLQDFVAKSKGQDIRAFVIGDRVVAAMQRSSNEGEFRANIHLGGKAQLIALT
HHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEEE
EAEQQLAVKAANAIGLSVAGVDLIRSDSGLLVLEVNASPGLEGIEQASGIDIAGLMIEHL
CHHHHHHHHHHHHHEEEEECEEEEECCCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHHH
IHSL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA