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The map label for this gene is trpA

Identifier: 126207954

GI number: 126207954

Start: 541649

End: 542458

Strand: Direct

Name: trpA

Synonym: APL_0470

Alternate gene names: 126207954

Gene position: 541649-542458 (Clockwise)

Preceding gene: 126207953

Following gene: 126207957

Centisome position: 23.81

GC content: 42.1

Gene sequence:

>810_bases
ATGAGCCGTTTTGATACTTTGTTTGCTAATTTAAAAGCGAAAAACGAAGGGGCATTTGTGCCGTTTGTTACCCTGTGTGA
TCCGGATTTTGATTGTTCGTTTGAAATTATCGAAACATTGATAGCCAACGGTGCGGATGCGTTAGAACTTGGTTTTCCAT
TTTCTGATCCTTTGTTAGACGGGCCGGTGATTCAAGCCGCAAATAAACGTGCATTAGACGGTGGATACAGCACGGATGCC
TGTTTTGAAATGATTGCAAAAATTCGCTCAAAATATCCTGAAATTCCAATCGGCTTGCTACTTTGTGCTAATTTGGTTTT
TGTACCAACTCAAGACGTATTTTTCAAACGTTGTGCCGAAACTGGCGTTGATGCGGTGTTAATTGCCGATGTGCCGGTAC
TTGCCGCTGAAGAATTTACTCAAGCAGCGAAAAAACACGGTATTCAATCAGTCTTTATTTGTCCGCCGAATGCGGATCAG
GCAACAATTGAACGTATTGCACGTTTAACTGAAGGCTATACCTATTTGGTTTCTCGTGCCGGTGTAACCAGTGCCGAGAA
CCAAGCTCACGCCAAAAATTTAGATAACCTTATTGAAAGCCTCAAGCGGTCAAATTCCGCCCCGATTTTGCAAGGCTTTG
GAATTGCTAAACCTGAACAAGTCAAAGAAGCATTAGTGCTTGGCTGTGACGGTGCGATTTCCGGATCGGCGATTGTGAAA
ATTATTGAACGGAATTTAGATAGCCAAACTCAATTACTTAGTGAATTAGCGAAATTTGTTAGCGTGATGAAAGCGGCAAC
AAAATCTTAA

Upstream 100 bases:

>100_bases
CAGAATCCGGATAAAGAACAACTGTTAGTCGTCAATCTTTCCGGTCGTGGCGATAAAGATATTTTTACCGTAGATAAAAT
TTTAAATGGGGGAAACTAAT

Downstream 100 bases:

>100_bases
CTAAAATATCTCAATGACAAGCGGTTAAATTCTCCGAAAAATTTGCAAAATTTAAAGGGGATTTAACCGCTTGTGTTTAA
GCTGCATTATGTAATTTGGC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSRFDTLFANLKAKNEGAFVPFVTLCDPDFDCSFEIIETLIANGADALELGFPFSDPLLDGPVIQAANKRALDGGYSTDA
CFEMIAKIRSKYPEIPIGLLLCANLVFVPTQDVFFKRCAETGVDAVLIADVPVLAAEEFTQAAKKHGIQSVFICPPNADQ
ATIERIARLTEGYTYLVSRAGVTSAENQAHAKNLDNLIESLKRSNSAPILQGFGIAKPEQVKEALVLGCDGAISGSAIVK
IIERNLDSQTQLLSELAKFVSVMKAATKS

Sequences:

>Translated_269_residues
MSRFDTLFANLKAKNEGAFVPFVTLCDPDFDCSFEIIETLIANGADALELGFPFSDPLLDGPVIQAANKRALDGGYSTDA
CFEMIAKIRSKYPEIPIGLLLCANLVFVPTQDVFFKRCAETGVDAVLIADVPVLAAEEFTQAAKKHGIQSVFICPPNADQ
ATIERIARLTEGYTYLVSRAGVTSAENQAHAKNLDNLIESLKRSNSAPILQGFGIAKPEQVKEALVLGCDGAISGSAIVK
IIERNLDSQTQLLSELAKFVSVMKAATKS
>Mature_268_residues
SRFDTLFANLKAKNEGAFVPFVTLCDPDFDCSFEIIETLIANGADALELGFPFSDPLLDGPVIQAANKRALDGGYSTDAC
FEMIAKIRSKYPEIPIGLLLCANLVFVPTQDVFFKRCAETGVDAVLIADVPVLAAEEFTQAAKKHGIQSVFICPPNADQA
TIERIARLTEGYTYLVSRAGVTSAENQAHAKNLDNLIESLKRSNSAPILQGFGIAKPEQVKEALVLGCDGAISGSAIVKI
IERNLDSQTQLLSELAKFVSVMKAATKS

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=269, Percent_Identity=57.2490706319703, Blast_Score=291, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6321412, Length=235, Percent_Identity=29.3617021276596, Blast_Score=118, Evalue=9e-28,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_ACTP2 (A3MZI8)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053179.1
- ProteinModelPortal:   A3MZI8
- SMR:   A3MZI8
- STRING:   A3MZI8
- GeneID:   4849907
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0470
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   A3MZI8
- ProtClustDB:   PRK13111
- BioCyc:   APLE416269:APL_0470-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28934; Mature: 28803

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFDTLFANLKAKNEGAFVPFVTLCDPDFDCSFEIIETLIANGADALELGFPFSDPLLD
CCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQAANKRALDGGYSTDACFEMIAKIRSKYPEIPIGLLLCANLVFVPTQDVFFKRCAE
CHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
TGVDAVLIADVPVLAAEEFTQAAKKHGIQSVFICPPNADQATIERIARLTEGYTYLVSRA
CCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHC
GVTSAENQAHAKNLDNLIESLKRSNSAPILQGFGIAKPEQVKEALVLGCDGAISGSAIVK
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHCCCCCCCHHHHHH
IIERNLDSQTQLLSELAKFVSVMKAATKS
HHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRFDTLFANLKAKNEGAFVPFVTLCDPDFDCSFEIIETLIANGADALELGFPFSDPLLD
CHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQAANKRALDGGYSTDACFEMIAKIRSKYPEIPIGLLLCANLVFVPTQDVFFKRCAE
CHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
TGVDAVLIADVPVLAAEEFTQAAKKHGIQSVFICPPNADQATIERIARLTEGYTYLVSRA
CCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHC
GVTSAENQAHAKNLDNLIESLKRSNSAPILQGFGIAKPEQVKEALVLGCDGAISGSAIVK
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHCCCCCCCHHHHHH
IIERNLDSQTQLLSELAKFVSVMKAATKS
HHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA