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The map label for this gene is trpB

Identifier: 126207953

GI number: 126207953

Start: 540457

End: 541647

Strand: Direct

Name: trpB

Synonym: APL_0469

Alternate gene names: 126207953

Gene position: 540457-541647 (Clockwise)

Preceding gene: 126207952

Following gene: 126207954

Centisome position: 23.76

GC content: 45.26

Gene sequence:

>1191_bases
ATGTCAGACACACTTCTTAATCCTTATTTCGGCGAATTTGGCGGAATGTATGTTCCGGAAATTCTCGTACCGGTCTTAAA
ACAACTCGAAGAGGCATTTGTTGCTGCGCAAAATGATCCGCTTTTCCAAGCGGAATTTACCGACTTACTGAAAAACTACG
CCGGTCGTCCGACCGCATTAACGCTTTGCCGTAATCTCACCAAAGGAAGTAAAACCAAACTTTATCTTAAACGTGAAGAT
CTGTTACATGGCGGCGCACATAAAACCAACCAAGTATTAGGACAAGCGTTATTAGCAAAACGTATGGGCAAAACTCGTAT
TATTGCGGAAACCGGTGCCGGACAACACGGGGTGGCAACCGCATTGGCATGTGCAATGTTAGATTTACCTTGTGTGATCT
ATATGGGTGCTAAAGATGTGGAACGCCAATCACCGAATGTATTCCGTATGCGTTTGATGGGTGCAGAAGTGATTCCGGTA
CAAAAAGGCTCTTGTTCATTAAAAGATGCCTGCTGTGAAGCAATGCGTGACTGGGCGGCAAACTATGAAACGACACATTA
TTTAATCGGTACGGCGGCAGGCCCGCACCCGTTCCCGACCATGGTGCGTGAATTTCAGAAAATGATCGGCGAAGAAACCA
AACGACAAATATTAGAAAAAGAGAACCGCTTACCGGATGCGGTGATTGCTGCGGTTGGCGGTGGCTCGAATGCCATCGGT
ATGTTTGCGGATTTTATTGAGGAAAAAAGCGTGCAGCTAATCGGTGTAGAACCGGCCGGCAAAGGCATTGAAACCGGCGA
ACACGGCGCACCGCTTAAACATGGTACCACCGGCATTTATTTCGGAATGAAATCGCCAATTATGCAAACTAAGGACGGAC
AAATCGAGGAATCTTATTCGATCTCGGCAGGTTTAGATTTCCCGTCAGTCGGGCCGCAACATGCTTACCTCAATTCAATT
GGACGAGCAGAGTATGTCTCAATTACCGACCAAGAAGCGTTAGATGCTTTCCAAGCATTAGCACAACATGAAGGTATTAT
TCCGGCATTAGAAAGTTCACATGCATTGGCTTATGCGCTGAAATTAATTGCACAGAATCCGGATAAAGAACAACTGTTAG
TCGTCAATCTTTCCGGTCGTGGCGATAAAGATATTTTTACCGTAGATAAAATTTTAAATGGGGGAAACTAA

Upstream 100 bases:

>100_bases
ATATTTCTCTTCTTTAATTCATCATAAATTTTTACTTGCATATCGTGAAGTTATGTACTATTTTAATAGTACATAATATT
TATTATAGAGAGAACACGTT

Downstream 100 bases:

>100_bases
TATGAGCCGTTTTGATACTTTGTTTGCTAATTTAAAAGCGAAAAACGAAGGGGCATTTGTGCCGTTTGTTACCCTGTGTG
ATCCGGATTTTGATTGTTCG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSDTLLNPYFGEFGGMYVPEILVPVLKQLEEAFVAAQNDPLFQAEFTDLLKNYAGRPTALTLCRNLTKGSKTKLYLKRED
LLHGGAHKTNQVLGQALLAKRMGKTRIIAETGAGQHGVATALACAMLDLPCVIYMGAKDVERQSPNVFRMRLMGAEVIPV
QKGSCSLKDACCEAMRDWAANYETTHYLIGTAAGPHPFPTMVREFQKMIGEETKRQILEKENRLPDAVIAAVGGGSNAIG
MFADFIEEKSVQLIGVEPAGKGIETGEHGAPLKHGTTGIYFGMKSPIMQTKDGQIEESYSISAGLDFPSVGPQHAYLNSI
GRAEYVSITDQEALDAFQALAQHEGIIPALESSHALAYALKLIAQNPDKEQLLVVNLSGRGDKDIFTVDKILNGGN

Sequences:

>Translated_396_residues
MSDTLLNPYFGEFGGMYVPEILVPVLKQLEEAFVAAQNDPLFQAEFTDLLKNYAGRPTALTLCRNLTKGSKTKLYLKRED
LLHGGAHKTNQVLGQALLAKRMGKTRIIAETGAGQHGVATALACAMLDLPCVIYMGAKDVERQSPNVFRMRLMGAEVIPV
QKGSCSLKDACCEAMRDWAANYETTHYLIGTAAGPHPFPTMVREFQKMIGEETKRQILEKENRLPDAVIAAVGGGSNAIG
MFADFIEEKSVQLIGVEPAGKGIETGEHGAPLKHGTTGIYFGMKSPIMQTKDGQIEESYSISAGLDFPSVGPQHAYLNSI
GRAEYVSITDQEALDAFQALAQHEGIIPALESSHALAYALKLIAQNPDKEQLLVVNLSGRGDKDIFTVDKILNGGN
>Mature_395_residues
SDTLLNPYFGEFGGMYVPEILVPVLKQLEEAFVAAQNDPLFQAEFTDLLKNYAGRPTALTLCRNLTKGSKTKLYLKREDL
LHGGAHKTNQVLGQALLAKRMGKTRIIAETGAGQHGVATALACAMLDLPCVIYMGAKDVERQSPNVFRMRLMGAEVIPVQ
KGSCSLKDACCEAMRDWAANYETTHYLIGTAAGPHPFPTMVREFQKMIGEETKRQILEKENRLPDAVIAAVGGGSNAIGM
FADFIEEKSVQLIGVEPAGKGIETGEHGAPLKHGTTGIYFGMKSPIMQTKDGQIEESYSISAGLDFPSVGPQHAYLNSIG
RAEYVSITDQEALDAFQALAQHEGIIPALESSHALAYALKLIAQNPDKEQLLVVNLSGRGDKDIFTVDKILNGGN

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=389, Percent_Identity=82.5192802056555, Blast_Score=675, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6321412, Length=385, Percent_Identity=52.4675324675325, Blast_Score=411, Evalue=1e-116,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_ACTP2 (A3MZI7)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053178.1
- ProteinModelPortal:   A3MZI7
- SMR:   A3MZI7
- STRING:   A3MZI7
- GeneID:   4849352
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0469
- eggNOG:   COG0133
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- PhylomeDB:   A3MZI7
- ProtClustDB:   PRK04346
- BioCyc:   APLE416269:APL_0469-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 42884; Mature: 42753

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTLLNPYFGEFGGMYVPEILVPVLKQLEEAFVAAQNDPLFQAEFTDLLKNYAGRPTAL
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHH
TLCRNLTKGSKTKLYLKREDLLHGGAHKTNQVLGQALLAKRMGKTRIIAETGAGQHGVAT
HHHHHCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
ALACAMLDLPCVIYMGAKDVERQSPNVFRMRLMGAEVIPVQKGSCSLKDACCEAMRDWAA
HHHHHHHHCCEEEEECCHHHHHCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHC
NYETTHYLIGTAAGPHPFPTMVREFQKMIGEETKRQILEKENRLPDAVIAAVGGGSNAIG
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCHHH
MFADFIEEKSVQLIGVEPAGKGIETGEHGAPLKHGTTGIYFGMKSPIMQTKDGQIEESYS
HHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCE
ISAGLDFPSVGPQHAYLNSIGRAEYVSITDQEALDAFQALAQHEGIIPALESSHALAYAL
EECCCCCCCCCCHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
KLIAQNPDKEQLLVVNLSGRGDKDIFTVDKILNGGN
HHHHCCCCCCCEEEEEECCCCCCCCEEHHHHHCCCC
>Mature Secondary Structure 
SDTLLNPYFGEFGGMYVPEILVPVLKQLEEAFVAAQNDPLFQAEFTDLLKNYAGRPTAL
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHH
TLCRNLTKGSKTKLYLKREDLLHGGAHKTNQVLGQALLAKRMGKTRIIAETGAGQHGVAT
HHHHHCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
ALACAMLDLPCVIYMGAKDVERQSPNVFRMRLMGAEVIPVQKGSCSLKDACCEAMRDWAA
HHHHHHHHCCEEEEECCHHHHHCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHC
NYETTHYLIGTAAGPHPFPTMVREFQKMIGEETKRQILEKENRLPDAVIAAVGGGSNAIG
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCHHH
MFADFIEEKSVQLIGVEPAGKGIETGEHGAPLKHGTTGIYFGMKSPIMQTKDGQIEESYS
HHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCE
ISAGLDFPSVGPQHAYLNSIGRAEYVSITDQEALDAFQALAQHEGIIPALESSHALAYAL
EECCCCCCCCCCHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
KLIAQNPDKEQLLVVNLSGRGDKDIFTVDKILNGGN
HHHHCCCCCCCEEEEEECCCCCCCCEEHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA