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The map label for this gene is rnhA

Identifier: 126207907

GI number: 126207907

Start: 480549

End: 481010

Strand: Direct

Name: rnhA

Synonym: APL_0423

Alternate gene names: 126207907

Gene position: 480549-481010 (Clockwise)

Preceding gene: 126207906

Following gene: 126207908

Centisome position: 21.13

GC content: 42.64

Gene sequence:

>462_bases
ATGAAATTAGTTGAAATATTTACGGACGGATCTTGCTTGGGCAATCCCGGCAAAGGCGGAATCGGCATTGTATTACGTTA
TAACGGACACGAGAAGCAAGTCAGCAAGGGTTACTTGCAGACGACCAATAATCGTATGGAATTGCGTGCGGTGATTGAAG
CGTTGGCAATGTTAAAAGAGCCTTGCCAAGTACAGTTGAATTCGGACAGCCAATATATGAAAGACGGCATTACCAAATGG
ATTTTTAGCTGGAAGAAAAACAACTGGAAAACCGCTAACGGTAAGCCGGTTAAAAATAAAGAGCTGTGGATCGCATTGGA
TCAAGAGATTCAACGTCATAAAATCGAATGGACTTGGGTAAAAGGCCATTCGGGTCATCGTGAAAATGAAATTTGCGATG
AGTTGGCTAAGGCGGGAGCAAATAACCCGACCTTAGAAGATATAGGATATAATGCGGATTAG

Upstream 100 bases:

>100_bases
AACTTAGAATCAATCAAAAGCCCCATAGTTTATGCTTTGGGGCTTTAATTTTTTCATCAGTTTTTAAATTAAGCGGTCAA
AAATTAAGAATGTTTTGCAA

Downstream 100 bases:

>100_bases
CCTAAAAGAGGCAAGCGGTCGAATATGATAAAATTTTTGCAAATTTGAACCGCTTGTAAATTTTTTGGCATATATTATGC
ATTTTTTACGATGTTTGCTC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MKLVEIFTDGSCLGNPGKGGIGIVLRYNGHEKQVSKGYLQTTNNRMELRAVIEALAMLKEPCQVQLNSDSQYMKDGITKW
IFSWKKNNWKTANGKPVKNKELWIALDQEIQRHKIEWTWVKGHSGHRENEICDELAKAGANNPTLEDIGYNAD

Sequences:

>Translated_153_residues
MKLVEIFTDGSCLGNPGKGGIGIVLRYNGHEKQVSKGYLQTTNNRMELRAVIEALAMLKEPCQVQLNSDSQYMKDGITKW
IFSWKKNNWKTANGKPVKNKELWIALDQEIQRHKIEWTWVKGHSGHRENEICDELAKAGANNPTLEDIGYNAD
>Mature_153_residues
MKLVEIFTDGSCLGNPGKGGIGIVLRYNGHEKQVSKGYLQTTNNRMELRAVIEALAMLKEPCQVQLNSDSQYMKDGITKW
IFSWKKNNWKTANGKPVKNKELWIALDQEIQRHKIEWTWVKGHSGHRENEICDELAKAGANNPTLEDIGYNAD

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=146, Percent_Identity=38.3561643835616, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1786408, Length=153, Percent_Identity=64.0522875816993, Blast_Score=209, Evalue=8e-56,
Organism=Caenorhabditis elegans, GI17505482, Length=132, Percent_Identity=37.1212121212121, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI115532542, Length=140, Percent_Identity=34.2857142857143, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17137476, Length=144, Percent_Identity=34.7222222222222, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45552509, Length=147, Percent_Identity=34.0136054421769, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_ACTP2 (A3MZE1)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053132.1
- ProteinModelPortal:   A3MZE1
- SMR:   A3MZE1
- STRING:   A3MZE1
- GeneID:   4849462
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0423
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   A3MZE1
- ProtClustDB:   PRK00203
- BioCyc:   APLE416269:APL_0423-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17386; Mature: 17386

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVEIFTDGSCLGNPGKGGIGIVLRYNGHEKQVSKGYLQTTNNRMELRAVIEALAMLKE
CEEEEEEECCCCCCCCCCCCEEEEEEECCCHHHHHCCCEECCCCHHHHHHHHHHHHHHCC
PCQVQLNSDSQYMKDGITKWIFSWKKNNWKTANGKPVKNKELWIALDQEIQRHKIEWTWV
CCEEEECCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCHHHHCEEEEEEE
KGHSGHRENEICDELAKAGANNPTLEDIGYNAD
ECCCCCCHHHHHHHHHHCCCCCCCHHCCCCCCC
>Mature Secondary Structure
MKLVEIFTDGSCLGNPGKGGIGIVLRYNGHEKQVSKGYLQTTNNRMELRAVIEALAMLKE
CEEEEEEECCCCCCCCCCCCEEEEEEECCCHHHHHCCCEECCCCHHHHHHHHHHHHHHCC
PCQVQLNSDSQYMKDGITKWIFSWKKNNWKTANGKPVKNKELWIALDQEIQRHKIEWTWV
CCEEEECCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCHHHHCEEEEEEE
KGHSGHRENEICDELAKAGANNPTLEDIGYNAD
ECCCCCCHHHHHHHHHHCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA