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The map label for this gene is argG [H]

Identifier: 126207906

GI number: 126207906

Start: 479096

End: 480427

Strand: Direct

Name: argG [H]

Synonym: APL_0422

Alternate gene names: 126207906

Gene position: 479096-480427 (Clockwise)

Preceding gene: 126207903

Following gene: 126207907

Centisome position: 21.06

GC content: 43.39

Gene sequence:

>1332_bases
ATGTCAGCAACAATTTTAGAATCTCTCCCGTTAGGTCAAAAAGTAGGTATCGCATTTTCAGGCGGTTTAGATACTTCAGC
AGCATTACTTTGGATGCGTAAAAAAGGTGCGGTGCCTTACGCTTACACTGCAAACTTAGGTCAGCCGGATGAAGACGATT
ACAACGCAATTCCGAAAAAAGCGATGGAATACGGTGCGGAAAACGCACGTTTAATCGACTGCCGTGCGCAATTGGCACAC
GAAGGTATTGCAGCGATTCAATGCGGTGCGTTCCATATTTCAACCGGCGGTATTCCGTATTTCAATACAACACCACTTGG
CCGTGCGGTAACCGGTACAATGCTTGTTGCGGCGATGAAAGAAGATGACGTAAACATTTGGGGTGACGGTTCAACTTTTA
AAGGTAACGATATCGAGCGTTTCTATCGTTACGGCTTATTAACCAACCCGAAATTAAAAATCTACAAACCTTGGTTAGAC
CAACTTTTCATTGATGAGCTTGGCGGCCGTTTCGAAATGTCTCAATTCTTAATCGAGAACGGTTTCGACTATAAAATGTC
GGTAGAAAAAGCATACTCGACAGACTCAAATATGTTAGGTGCGACTCACGAAGCGAAAGACTTAGAAGAATTAAGCACCG
GTATGAAAATCGTGAAACCGATTATGGGCGTAGCATTCTGGGACGAATCTGTAGAAATTAAACCTGAAACCGTTTCGGTA
ACCTTCGAAGAAGGTGTGCCGGTAGCATTAAACGGTAAACGTATCGAAGACGCGGTTGAATTAATCTTAGAAGCAAACCG
TATCGGCGGTCGCCACGGTTTAGGTATGTCGGATCAAATCGAAAACCGTATTATCGAAGCGAAATCTCGCGGTATCTATG
AAGCACCAGGGATGGCGTTATTACACATCGCTTACGAGCGTTTAGTAACCGGTATCCATAACGAAGATACAATCGAACAA
TACCGCATTAACGGTTTACGTTTAGGCCGTTTACTTTACCAAGGTCGTTGGTTCGATCCGCAAGCGTTAATGTTACGCGA
AACGGCTCAACGTTGGGTGGCAAAAGCGATTACCGGTACCGTTACTTTAGAATTACGTCGTGGTAATGATTTCACGATTT
TAAATACCGAGTCACCAAACTTAACTTACGAAGCAGAACGTTTAAGTATGGAAAAAGTAGAAGATGCACCGTTCACACCG
GCGGATCGTATCGGTCAATTAACTATGCGTAACCTAGATATCGTGGATACGCGCGGTAAATTAGGTATCTATACCGAAAC
GGGTTTATTATCCGTTCAAAATAACGTATTACCGCAACTTGGTAAAAAGTAA

Upstream 100 bases:

>100_bases
GATGTAATTTTTACGAAATTGAACTAGAATAGACGGAATGGCACAAATGCAACCGTTTGCGTCAGTTTTCTTTTATGTTT
TAAAATTTATAGGAATTCGT

Downstream 100 bases:

>100_bases
TCTTGATCTAATGCTTTAGAAAACTTAGAATCAATCAAAAGCCCCATAGTTTATGCTTTGGGGCTTTAATTTTTTCATCA
GTTTTTAAATTAAGCGGTCA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSATILESLPLGQKVGIAFSGGLDTSAALLWMRKKGAVPYAYTANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAH
EGIAAIQCGAFHISTGGIPYFNTTPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPKLKIYKPWLD
QLFIDELGGRFEMSQFLIENGFDYKMSVEKAYSTDSNMLGATHEAKDLEELSTGMKIVKPIMGVAFWDESVEIKPETVSV
TFEEGVPVALNGKRIEDAVELILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQ
YRINGLRLGRLLYQGRWFDPQALMLRETAQRWVAKAITGTVTLELRRGNDFTILNTESPNLTYEAERLSMEKVEDAPFTP
ADRIGQLTMRNLDIVDTRGKLGIYTETGLLSVQNNVLPQLGKK

Sequences:

>Translated_443_residues
MSATILESLPLGQKVGIAFSGGLDTSAALLWMRKKGAVPYAYTANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAH
EGIAAIQCGAFHISTGGIPYFNTTPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPKLKIYKPWLD
QLFIDELGGRFEMSQFLIENGFDYKMSVEKAYSTDSNMLGATHEAKDLEELSTGMKIVKPIMGVAFWDESVEIKPETVSV
TFEEGVPVALNGKRIEDAVELILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQ
YRINGLRLGRLLYQGRWFDPQALMLRETAQRWVAKAITGTVTLELRRGNDFTILNTESPNLTYEAERLSMEKVEDAPFTP
ADRIGQLTMRNLDIVDTRGKLGIYTETGLLSVQNNVLPQLGKK
>Mature_442_residues
SATILESLPLGQKVGIAFSGGLDTSAALLWMRKKGAVPYAYTANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHE
GIAAIQCGAFHISTGGIPYFNTTPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPKLKIYKPWLDQ
LFIDELGGRFEMSQFLIENGFDYKMSVEKAYSTDSNMLGATHEAKDLEELSTGMKIVKPIMGVAFWDESVEIKPETVSVT
FEEGVPVALNGKRIEDAVELILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQY
RINGLRLGRLLYQGRWFDPQALMLRETAQRWVAKAITGTVTLELRRGNDFTILNTESPNLTYEAERLSMEKVEDAPFTPA
DRIGQLTMRNLDIVDTRGKLGIYTETGLLSVQNNVLPQLGKK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=406, Percent_Identity=28.3251231527094, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI16950633, Length=406, Percent_Identity=28.3251231527094, Blast_Score=145, Evalue=6e-35,
Organism=Escherichia coli, GI1789563, Length=428, Percent_Identity=80.8411214953271, Blast_Score=716, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324514, Length=418, Percent_Identity=27.2727272727273, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI21358151, Length=425, Percent_Identity=26.3529411764706, Blast_Score=148, Evalue=6e-36,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 49228; Mature: 49097

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATILESLPLGQKVGIAFSGGLDTSAALLWMRKKGAVPYAYTANLGQPDEDDYNAIPKK
CCCCHHHHCCCCCEECEEEECCCCCHHHEEEEHHCCCCCEEEECCCCCCCCCCHHHCCHH
AMEYGAENARLIDCRAQLAHEGIAAIQCGAFHISTGGIPYFNTTPLGRAVTGTMLVAAMK
HHHCCCCCCEEEHHHHHHHHCCCEEEEECEEEEECCCCCCCCCCCCCHHHHHHHEEEEEC
EDDVNIWGDGSTFKGNDIERFYRYGLLTNPKLKIYKPWLDQLFIDELGGRFEMSQFLIEN
CCCCEEEECCCCCCCCCHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHC
GFDYKMSVEKAYSTDSNMLGATHEAKDLEELSTGMKIVKPIMGVAFWDESVEIKPETVSV
CCCEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCEEECCCEEEE
TFEEGVPVALNGKRIEDAVELILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMAL
EECCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHH
LHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQGRWFDPQALMLRETAQRWVAKAITGT
HHHHHHHHHHCCCCCCHHHHHHHCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHCEE
VTLELRRGNDFTILNTESPNLTYEAERLSMEKVEDAPFTPADRIGQLTMRNLDIVDTRGK
EEEEEECCCCEEEEECCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHCCEEEEECCCC
LGIYTETGLLSVQNNVLPQLGKK
EEEEECCCEEEECCCHHHHCCCC
>Mature Secondary Structure 
SATILESLPLGQKVGIAFSGGLDTSAALLWMRKKGAVPYAYTANLGQPDEDDYNAIPKK
CCCHHHHCCCCCEECEEEECCCCCHHHEEEEHHCCCCCEEEECCCCCCCCCCHHHCCHH
AMEYGAENARLIDCRAQLAHEGIAAIQCGAFHISTGGIPYFNTTPLGRAVTGTMLVAAMK
HHHCCCCCCEEEHHHHHHHHCCCEEEEECEEEEECCCCCCCCCCCCCHHHHHHHEEEEEC
EDDVNIWGDGSTFKGNDIERFYRYGLLTNPKLKIYKPWLDQLFIDELGGRFEMSQFLIEN
CCCCEEEECCCCCCCCCHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHC
GFDYKMSVEKAYSTDSNMLGATHEAKDLEELSTGMKIVKPIMGVAFWDESVEIKPETVSV
CCCEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCEEECCCEEEE
TFEEGVPVALNGKRIEDAVELILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMAL
EECCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHH
LHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQGRWFDPQALMLRETAQRWVAKAITGT
HHHHHHHHHHCCCCCCHHHHHHHCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHCEE
VTLELRRGNDFTILNTESPNLTYEAERLSMEKVEDAPFTPADRIGQLTMRNLDIVDTRGK
EEEEEECCCCEEEEECCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHCCEEEEECCCC
LGIYTETGLLSVQNNVLPQLGKK
EEEEECCCEEEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA