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The map label for this gene is rsuA [H]

Identifier: 126207838

GI number: 126207838

Start: 397182

End: 397874

Strand: Direct

Name: rsuA [H]

Synonym: APL_0354

Alternate gene names: 126207838

Gene position: 397182-397874 (Clockwise)

Preceding gene: 126207837

Following gene: 126207839

Centisome position: 17.46

GC content: 42.71

Gene sequence:

>693_bases
ATGCGTTTAGATAAATTTATTGCCGAAAATACGGGATTAACTCGTTCTCAAGCGGCAAGAGCCTTAAAAAGCGGTTTAGT
TACCATAAACGGAAAGTTAGAAAAAAGCGGTTCGGTCAAAATTGGTGATACGGATGAAATTTGTTACGAAGGCGAACGTT
TGGAATGGGTCGATGAAGGTCAATACTTTATGCTATATAAGCCGCAAGGCTATGTGTGTTCGCATGATGACGGCGAATAT
CCGACCGTATTCCAATTTTTTGACTATCCGCTGATGACCAAACTGCATACCGCAGGGCGTTTGGATGTGGATACGACCGG
TTTGGTGTTATTAACCGATGACGGTAAATGGTCGCATCGCATCACCTCGCCGAAACATCATTGTGAAAAGACCTATTTGG
TGACGCTTGCCGATCCGGTGGAAGATTTTTATGCGAAAAAATTAGCGGAAGGCATTTTATTACGTGGCGAACGCGAGCCT
TGCTTACCGGCGAAAATGGAAGTTATTGACGATTACAATGTGAATTTAACCATTAGTGAAGGGCGTTATCATCAAGTAAA
ACGGATGTTTGCTGCATTAGGTAATAAAGTGGAAGCGTTACATCGTTGGCGTATCGGTGATGTGGTTTTAGACGAAAATC
TTGAAGAAGGCGAATATCGTCCGCTTAGCGAACAAGAAGTGGAGAGTTTTTAA

Upstream 100 bases:

>100_bases
TATAAGAAATCAAGTGCCAATTAGGTTATTTTCTGCGGAAAAAGTTCACGCTTCACCACGCTAAAGCGTATAATAAAACA
GTATAACAAAAGAGGAAAAT

Downstream 100 bases:

>100_bases
TGGTAACACAAAGACCGCATCCGTGCTTTTTTATTATTCTCGGTATGATGGCGATGTTACCGCCTTTAGCGATTGATATG
TATTTGCCGTCGTTTCTTGA

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MRLDKFIAENTGLTRSQAARALKSGLVTINGKLEKSGSVKIGDTDEICYEGERLEWVDEGQYFMLYKPQGYVCSHDDGEY
PTVFQFFDYPLMTKLHTAGRLDVDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADPVEDFYAKKLAEGILLRGEREP
CLPAKMEVIDDYNVNLTISEGRYHQVKRMFAALGNKVEALHRWRIGDVVLDENLEEGEYRPLSEQEVESF

Sequences:

>Translated_230_residues
MRLDKFIAENTGLTRSQAARALKSGLVTINGKLEKSGSVKIGDTDEICYEGERLEWVDEGQYFMLYKPQGYVCSHDDGEY
PTVFQFFDYPLMTKLHTAGRLDVDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADPVEDFYAKKLAEGILLRGEREP
CLPAKMEVIDDYNVNLTISEGRYHQVKRMFAALGNKVEALHRWRIGDVVLDENLEEGEYRPLSEQEVESF
>Mature_230_residues
MRLDKFIAENTGLTRSQAARALKSGLVTINGKLEKSGSVKIGDTDEICYEGERLEWVDEGQYFMLYKPQGYVCSHDDGEY
PTVFQFFDYPLMTKLHTAGRLDVDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADPVEDFYAKKLAEGILLRGEREP
CLPAKMEVIDDYNVNLTISEGRYHQVKRMFAALGNKVEALHRWRIGDVVLDENLEEGEYRPLSEQEVESF

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=229, Percent_Identity=56.3318777292576, Blast_Score=271, Evalue=3e-74,
Organism=Escherichia coli, GI1787524, Length=226, Percent_Identity=29.2035398230088, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790453, Length=228, Percent_Identity=29.3859649122807, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI87081838, Length=150, Percent_Identity=32.6666666666667, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26287; Mature: 26287

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFIAENTGLTRSQAARALKSGLVTINGKLEKSGSVKIGDTDEICYEGERLEWVDEG
CCCHHHHHHCCCCCHHHHHHHHHCCEEEEECEECCCCCEEECCCHHHHCCCCEEEEEECC
QYFMLYKPQGYVCSHDDGEYPTVFQFFDYPLMTKLHTAGRLDVDTTGLVLLTDDGKWSHR
CEEEEECCCCEEECCCCCCCCEEHHHHCCHHHHHHHCCCCCEECCCCEEEEECCCCCCCC
ITSPKHHCEKTYLVTLADPVEDFYAKKLAEGILLRGEREPCLPAKMEVIDDYNVNLTISE
CCCCHHHCCEEEEEEECCHHHHHHHHHHHHHHEEECCCCCCCCCHHHEECCCCEEEEECC
GRYHQVKRMFAALGNKVEALHRWRIGDVVLDENLEEGEYRPLSEQEVESF
CHHHHHHHHHHHHCCHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHCC
>Mature Secondary Structure
MRLDKFIAENTGLTRSQAARALKSGLVTINGKLEKSGSVKIGDTDEICYEGERLEWVDEG
CCCHHHHHHCCCCCHHHHHHHHHCCEEEEECEECCCCCEEECCCHHHHCCCCEEEEEECC
QYFMLYKPQGYVCSHDDGEYPTVFQFFDYPLMTKLHTAGRLDVDTTGLVLLTDDGKWSHR
CEEEEECCCCEEECCCCCCCCEEHHHHCCHHHHHHHCCCCCEECCCCEEEEECCCCCCCC
ITSPKHHCEKTYLVTLADPVEDFYAKKLAEGILLRGEREPCLPAKMEVIDDYNVNLTISE
CCCCHHHCCEEEEEEECCHHHHHHHHHHHHHHEEECCCCCCCCCHHHEECCCCEEEEECC
GRYHQVKRMFAALGNKVEALHRWRIGDVVLDENLEEGEYRPLSEQEVESF
CHHHHHHHHHHHHCCHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]