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The map label for this gene is pyrE

Identifier: 126207802

GI number: 126207802

Start: 354902

End: 355543

Strand: Direct

Name: pyrE

Synonym: APL_0318

Alternate gene names: 126207802

Gene position: 354902-355543 (Clockwise)

Preceding gene: 126207801

Following gene: 126207803

Centisome position: 15.6

GC content: 42.21

Gene sequence:

>642_bases
ATGGAACAATATAAACACGATTTTATTGAATTTGCATTAAGCCGTAACGTTTTAAAATTCGGCGAATTTACCCTGAAATC
AGGCAGAAAAAGCCCGTATTTCTTTAATGCGGGTTTATTTAATACCGGTAGAGATCTTGCCAAACTCGGTGAGTTTTATG
CGCAGGCGATTCAAGCAAGCGGTCTAAATTTCGACGTACTTTTCGGACCGGCTTATAAAGGTATCCCGATTGCAACGACG
GTTGCGGTTGCGTTAGTTAATCAATTTGATGTGGATAAGCCTTGTTGCTTTAACCGTAAAGAAGCGAAAGATCACGGCGA
GGGCGGTAATCTTATCGGTTCGCCGTTGAAAGGCAGAATCTTATTAGTTGATGACGTGATTACCGCCGGTACGGCAATCC
GTGAATCGATGGAAATCATTAATGCGAATCAAGCGGAATTAGCAGGCGTATTGATTGCGTTAAATCGTAAAGAAAAGGGC
AAAGGCGAGCTTTCCGCAATTCAAGAAGTCGAACGTGATTACGGCTGCCAAGTTTTCTCGATTATCGATTTTGACGATTT
AATTCAATTTATTGAAAAATCGGAACAATACGCACCGTATTTAGCTTCAATGCGTGCCTATCGCGAGCAGTACGGCGTGT
AA

Upstream 100 bases:

>100_bases
TCACGCCGGAATAATTTGATTAGCCGCAGATAACGCAGATAAACGGAAGTTTCCGTGAAAATTGGTGTTGTCTGTGGTTT
TTTTTATATAAGGAAACAAT

Downstream 100 bases:

>100_bases
GAATCTCGAAATTGATTATGAATTAATTTCATAAAAAATCGAAAAAGTTTCACATTAATTTGAATGATTTCCCTTGAGTG
ATGCAGTTCGTTCGCTTACG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MEQYKHDFIEFALSRNVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLAKLGEFYAQAIQASGLNFDVLFGPAYKGIPIATT
VAVALVNQFDVDKPCCFNRKEAKDHGEGGNLIGSPLKGRILLVDDVITAGTAIRESMEIINANQAELAGVLIALNRKEKG
KGELSAIQEVERDYGCQVFSIIDFDDLIQFIEKSEQYAPYLASMRAYREQYGV

Sequences:

>Translated_213_residues
MEQYKHDFIEFALSRNVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLAKLGEFYAQAIQASGLNFDVLFGPAYKGIPIATT
VAVALVNQFDVDKPCCFNRKEAKDHGEGGNLIGSPLKGRILLVDDVITAGTAIRESMEIINANQAELAGVLIALNRKEKG
KGELSAIQEVERDYGCQVFSIIDFDDLIQFIEKSEQYAPYLASMRAYREQYGV
>Mature_213_residues
MEQYKHDFIEFALSRNVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLAKLGEFYAQAIQASGLNFDVLFGPAYKGIPIATT
VAVALVNQFDVDKPCCFNRKEAKDHGEGGNLIGSPLKGRILLVDDVITAGTAIRESMEIINANQAELAGVLIALNRKEKG
KGELSAIQEVERDYGCQVFSIIDFDDLIQFIEKSEQYAPYLASMRAYREQYGV

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=180, Percent_Identity=27.7777777777778, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1790073, Length=213, Percent_Identity=72.3004694835681, Blast_Score=325, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17508631, Length=162, Percent_Identity=31.4814814814815, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17554964, Length=190, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323530, Length=221, Percent_Identity=47.9638009049774, Blast_Score=188, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6323927, Length=223, Percent_Identity=44.3946188340807, Blast_Score=178, Evalue=7e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_ACTP2 (A3MZ36)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053027.1
- ProteinModelPortal:   A3MZ36
- SMR:   A3MZ36
- STRING:   A3MZ36
- GeneID:   4850389
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0318
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   FAFNRKE
- PhylomeDB:   A3MZ36
- ProtClustDB:   PRK00455
- BioCyc:   APLE416269:APL_0318-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 23684; Mature: 23684

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 26-26 BINDING 99-99 BINDING 100-100 BINDING 103-103 BINDING 105-105 BINDING 128-128 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQYKHDFIEFALSRNVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLAKLGEFYAQAIQAS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
GLNFDVLFGPAYKGIPIATTVAVALVNQFDVDKPCCFNRKEAKDHGEGGNLIGSPLKGRI
CCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCE
LLVDDVITAGTAIRESMEIINANQAELAGVLIALNRKEKGKGELSAIQEVERDYGCQVFS
EEEECHHHHHHHHHHHHHHHCCCHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEE
IIDFDDLIQFIEKSEQYAPYLASMRAYREQYGV
ECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEQYKHDFIEFALSRNVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLAKLGEFYAQAIQAS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
GLNFDVLFGPAYKGIPIATTVAVALVNQFDVDKPCCFNRKEAKDHGEGGNLIGSPLKGRI
CCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCE
LLVDDVITAGTAIRESMEIINANQAELAGVLIALNRKEKGKGELSAIQEVERDYGCQVFS
EEEECHHHHHHHHHHHHHHHCCCHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEE
IIDFDDLIQFIEKSEQYAPYLASMRAYREQYGV
ECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA