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The map label for this gene is ruvB

Identifier: 126207772

GI number: 126207772

Start: 313779

End: 314663

Strand: Direct

Name: ruvB

Synonym: APL_0284

Alternate gene names: 126207772

Gene position: 313779-314663 (Clockwise)

Preceding gene: 126207771

Following gene: 126207774

Centisome position: 13.8

GC content: 45.2

Gene sequence:

>885_bases
ATGGAGATCTTTATTAAAGCGGCAAAATTGCGTGATGAGGCGCTAGATCACTTATTAATTTTTGGTCCGCCGGGATTGGG
TAAAACCACCCTAGCTAATATTGTTGCCAACGAAATGGGCGTGAATATTCGCACAACTTCCGGACCTGTGTTAGAGAAAG
CGGGCGATCTAGCGGCAATGCTGACTAATTTAGAGCCTTATGATGTGTTATTTATTGATGAGATCCACCGCTTATCACCG
GCAATTGAAGAAGTGCTTTATCCGGCGATGGAAGATTATCAGTTGGATATTATGATTGGCGAAGGGCCGGCGGCACGTTC
AATTAAACTAGATTTACCGCCGTTTACCTTAGTGGGAGCAACCACCCGTGCCGGTTCACTGACTTCTCCGTTACGCGACC
GTTTTGGAATTGTACAGCGTTTAGAGTTTTACTCGGTAGATGATTTAACCTCGATCGTAAAACGTAGTGCCGATTGCTTA
AACTTAAATTTATCGCCGGACGGTGCTTATGAAGTGGCTCGCCGTTCACGTGGCACCCCTCGAATTGCTAACCGTTTATT
ACGCCGTGTGCGAGATTATGCCGATGTGCGTAATAACGGTGTGATTACCTCTGATATTGCTAAACAAGCGTTGGCGATGC
TTGATGTAGATTCCGAAGGCTTTGACTTTATGGACATCAAATTATTGCAAGCGATTGTAGAACGCTTTGACGGAGGCCCG
GTCGGCTTAGATAATTTAGCTGCAGCGATAGGTGAAGAGCGAGATACGATTGAAGACGTACTTGAACCTTATTTGATTCA
GCAGGGTTTTTTACAGCGAACGCCACGAGGGCGTATTGCTACTAGCCGAACTTATGCACATTTAGGCATTGCAAAATTAG
ATTAA

Upstream 100 bases:

>100_bases
AATTAGTGCCTCGCCGAAGCGTGAGGAAGAAGTCATCGATCGTGCGATTCGTCCTAAATTATTAGCGGATTATGTCGGTC
AGCCGTCTGTACGAGAGCAG

Downstream 100 bases:

>100_bases
ATACAAGCGGTTGTTTTTGTAAGATTTTAGGCAAAAATAACCGCTTGTTTTCTAAGCTATTTGAGCATTGCAATATAATT
GCAACTTACATCATTATTCA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MEIFIKAAKLRDEALDHLLIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLTNLEPYDVLFIDEIHRLSP
AIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGATTRAGSLTSPLRDRFGIVQRLEFYSVDDLTSIVKRSADCL
NLNLSPDGAYEVARRSRGTPRIANRLLRRVRDYADVRNNGVITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERFDGGP
VGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIATSRTYAHLGIAKLD

Sequences:

>Translated_294_residues
MEIFIKAAKLRDEALDHLLIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLTNLEPYDVLFIDEIHRLSP
AIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGATTRAGSLTSPLRDRFGIVQRLEFYSVDDLTSIVKRSADCL
NLNLSPDGAYEVARRSRGTPRIANRLLRRVRDYADVRNNGVITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERFDGGP
VGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIATSRTYAHLGIAKLD
>Mature_294_residues
MEIFIKAAKLRDEALDHLLIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLTNLEPYDVLFIDEIHRLSP
AIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGATTRAGSLTSPLRDRFGIVQRLEFYSVDDLTSIVKRSADCL
NLNLSPDGAYEVARRSRGTPRIANRLLRRVRDYADVRNNGVITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERFDGGP
VGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIATSRTYAHLGIAKLD

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=290, Percent_Identity=83.1034482758621, Blast_Score=493, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_ACTP2 (A3MZ06)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052997.1
- ProteinModelPortal:   A3MZ06
- SMR:   A3MZ06
- STRING:   A3MZ06
- GeneID:   4849172
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0284
- eggNOG:   COG2255
- HOGENOM:   HBG339960
- ProtClustDB:   PRK00080
- BioCyc:   APLE416269:APL_0284-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 32368; Mature: 32368

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIFIKAAKLRDEALDHLLIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAM
CCEEEEHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHH
LTNLEPYDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
HHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCEEEEEC
TTRAGSLTSPLRDRFGIVQRLEFYSVDDLTSIVKRSADCLNLNLSPDGAYEVARRSRGTP
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCH
RIANRLLRRVRDYADVRNNGVITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERFDGGP
HHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
VGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIATSRTYAHLGIAKLD
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCEEECC
>Mature Secondary Structure
MEIFIKAAKLRDEALDHLLIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAM
CCEEEEHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHH
LTNLEPYDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
HHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCEEEEEC
TTRAGSLTSPLRDRFGIVQRLEFYSVDDLTSIVKRSADCLNLNLSPDGAYEVARRSRGTP
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCH
RIANRLLRRVRDYADVRNNGVITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERFDGGP
HHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
VGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIATSRTYAHLGIAKLD
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA