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The map label for this gene is ruvA

Identifier: 126207771

GI number: 126207771

Start: 313035

End: 313640

Strand: Direct

Name: ruvA

Synonym: APL_0283

Alternate gene names: 126207771

Gene position: 313035-313640 (Clockwise)

Preceding gene: 126207770

Following gene: 126207772

Centisome position: 13.76

GC content: 40.26

Gene sequence:

>606_bases
ATGATCGGAAGATTACACGGTAAAATTATTGAAAAACAACCGCCTGAAATGGTAATTGATGTCCAAGGTGTCGGGTATGA
AGTGCTATTGCCGATGACGAGCTTTTACAGCTTGCCGCAAGTAGGTGAAGAAGCCACTATTTTTACGCATTTAGTCGTAC
GTGAAGATGCACATTTATTATTTGGTTTTGCACAAAAGCAAGATAGAACTTTATTTCGTGAATTGATTAAAACCAACGGT
GTAGGGCCTAAATTAGCCCTTGCGATTTTATCAGCAATGTCAGTTTCACAATTTGCCAATGCTGTTGAAAATGAAGAACT
TGCCAAATTAACTAAAATTCCGGGCATTGGGCGTAAGACAGCCGAGCGTTTATTAGTAGAACTCAAAGGCAAATTCAAAG
GTGTGGCACAAAGTGATTTCTTTGAAGAGCATTCGGTTGAAACGATTGTTGCAACACATTCGCACGATCCGGCAGATGAA
GCCCGTGATGCTTTAGTAGCGTTAGGCTATAAACTCGCTGATGCTGAGAAGATGATTAAGAAAGTGAATAAAGCGGGTGC
AACCAGCGAACAATTAATTCGTGAAGCATTAAAAGCCTCTCTTTAA

Upstream 100 bases:

>100_bases
TTTTTGCAAAAAATTAGCTGAAATAGACCGCTTGTTAGCCTGTTATTCTATACAGTTTTCTGTTATGCTAAGGCAAATTT
TTTAGTTTGGAATAAAATCA

Downstream 100 bases:

>100_bases
AAAGTAAAAATACCTTAAATGATTGAAGCAGATAGAATAATTAGTGCCTCGCCGAAGCGTGAGGAAGAAGTCATCGATCG
TGCGATTCGTCCTAAATTAT

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MIGRLHGKIIEKQPPEMVIDVQGVGYEVLLPMTSFYSLPQVGEEATIFTHLVVREDAHLLFGFAQKQDRTLFRELIKTNG
VGPKLALAILSAMSVSQFANAVENEELAKLTKIPGIGRKTAERLLVELKGKFKGVAQSDFFEEHSVETIVATHSHDPADE
ARDALVALGYKLADAEKMIKKVNKAGATSEQLIREALKASL

Sequences:

>Translated_201_residues
MIGRLHGKIIEKQPPEMVIDVQGVGYEVLLPMTSFYSLPQVGEEATIFTHLVVREDAHLLFGFAQKQDRTLFRELIKTNG
VGPKLALAILSAMSVSQFANAVENEELAKLTKIPGIGRKTAERLLVELKGKFKGVAQSDFFEEHSVETIVATHSHDPADE
ARDALVALGYKLADAEKMIKKVNKAGATSEQLIREALKASL
>Mature_201_residues
MIGRLHGKIIEKQPPEMVIDVQGVGYEVLLPMTSFYSLPQVGEEATIFTHLVVREDAHLLFGFAQKQDRTLFRELIKTNG
VGPKLALAILSAMSVSQFANAVENEELAKLTKIPGIGRKTAERLLVELKGKFKGVAQSDFFEEHSVETIVATHSHDPADE
ARDALVALGYKLADAEKMIKKVNKAGATSEQLIREALKASL

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=204, Percent_Identity=62.2549019607843, Blast_Score=244, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_ACTP2 (A3MZ05)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052996.1
- ProteinModelPortal:   A3MZ05
- SMR:   A3MZ05
- STRING:   A3MZ05
- GeneID:   4848753
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0283
- eggNOG:   COG0632
- HOGENOM:   HBG635309
- OMA:   LFGFAQK
- PhylomeDB:   A3MZ05
- ProtClustDB:   PRK00116
- BioCyc:   APLE416269:APL_0283-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 22066; Mature: 22066

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGRLHGKIIEKQPPEMVIDVQGVGYEVLLPMTSFYSLPQVGEEATIFTHLVVREDAHLL
CCCCCCCHHHCCCCCCEEEEECCCCEEEEHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHH
FGFAQKQDRTLFRELIKTNGVGPKLALAILSAMSVSQFANAVENEELAKLTKIPGIGRKT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHH
AERLLVELKGKFKGVAQSDFFEEHSVETIVATHSHDPADEARDALVALGYKLADAEKMIK
HHHHHHHHHHHHCCCHHHHHHHHHCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
KVNKAGATSEQLIREALKASL
HHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIGRLHGKIIEKQPPEMVIDVQGVGYEVLLPMTSFYSLPQVGEEATIFTHLVVREDAHLL
CCCCCCCHHHCCCCCCEEEEECCCCEEEEHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHH
FGFAQKQDRTLFRELIKTNGVGPKLALAILSAMSVSQFANAVENEELAKLTKIPGIGRKT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHH
AERLLVELKGKFKGVAQSDFFEEHSVETIVATHSHDPADEARDALVALGYKLADAEKMIK
HHHHHHHHHHHHCCCHHHHHHHHHCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
KVNKAGATSEQLIREALKASL
HHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA