Click here to switch to the map view.

The map label for this gene is potB [H]

Identifier: 126207769

GI number: 126207769

Start: 311260

End: 312126

Strand: Direct

Name: potB [H]

Synonym: APL_0281

Alternate gene names: 126207769

Gene position: 311260-312126 (Clockwise)

Preceding gene: 126207768

Following gene: 126207770

Centisome position: 13.68

GC content: 39.33

Gene sequence:

>867_bases
ATGAAGATTGCTAACAACAAATTCCAAGCCGGAACGGTTTCGGTTATCTTCGGTTGGTTGTTGCTTTTTGTACTCGTACC
GAACGTATTGGTACTGTTAATCAGCTTTTTAACCGAAAACCGCCAAAGCGCATACTTTGTAGATTTCATCTTTAGCCTTG
ACGCATATACGCAGTTGTTCAACGATACCTATGCAACGGTATTATGGAACTCGTTTAAAATGTCGGCGATAGCAACGTTT
TTCTGTTTAATCATCGGTTATCCGTTTGCATTTATTATTGCCAAAATGCCGGCGAAAATTCGCCCGATTTTGTTATTTTT
AGTCGTATTGCCGTTTTGGACAAACTCGCTGATCCGTATCTACGGCATTAAAATTTTCTTAGGCGTGAAGGGGGTATTAA
ACGAGGCGTTATTGGCAATCGGCTTAATTGACGAACCGCTTCGTTTATTAAACTCCGAATTGGCGATTATTATCGGTTTA
GTATATATCTTGTTACCGTTTATGATTTTACCGCTTTATTCTTCTATCGAGAAAATTGACGGTCGTTTATTAGAAGCGGC
AAAAGATTTAGGTGCGAATGCGTTCCAACGTTTTGTGCGTGTAACCATTCCGCTGACAATGCCGGGGATTGTGGCAGGTT
GTTTATTAGTATTATTACCGGCAATGGGAATGTTCTATGTTGCCGACTTATTAGGCGGCGGTAAAACACCGTTAATCGGT
AATATTATTAAGAGCTTGTTCTTAAATACCAACCAGTTTGCATTAGGTTCGGCGGTTAGTATCGCCCTTACCGTGTTAAT
GGCGTTAATGTTATATGTTTATTACCGTGCAAATAAATTATTAAATAAAAAGGTGGAGCTTGAATAA

Upstream 100 bases:

>100_bases
GTAAGTGAATTCTTTAACGAAGATGATCCGAATATCGATCATGAAGTTGGTCAAAAAGTGGCATTAACTTGGTATGAAGG
TTGGGAGGTAGTACTCAACG

Downstream 100 bases:

>100_bases
TGAAAAAGACCTTAAGAAACTTTTTTATGTTCGTGGTGTTTGCCTACCTTTATATTCCGATTATTATTTTGGTGGTAAAC
TCATTTAACGAAGACCGTTA

Product: spermidine/putrescine ABC transporter membrane protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKIANNKFQAGTVSVIFGWLLLFVLVPNVLVLLISFLTENRQSAYFVDFIFSLDAYTQLFNDTYATVLWNSFKMSAIATF
FCLIIGYPFAFIIAKMPAKIRPILLFLVVLPFWTNSLIRIYGIKIFLGVKGVLNEALLAIGLIDEPLRLLNSELAIIIGL
VYILLPFMILPLYSSIEKIDGRLLEAAKDLGANAFQRFVRVTIPLTMPGIVAGCLLVLLPAMGMFYVADLLGGGKTPLIG
NIIKSLFLNTNQFALGSAVSIALTVLMALMLYVYYRANKLLNKKVELE

Sequences:

>Translated_288_residues
MKIANNKFQAGTVSVIFGWLLLFVLVPNVLVLLISFLTENRQSAYFVDFIFSLDAYTQLFNDTYATVLWNSFKMSAIATF
FCLIIGYPFAFIIAKMPAKIRPILLFLVVLPFWTNSLIRIYGIKIFLGVKGVLNEALLAIGLIDEPLRLLNSELAIIIGL
VYILLPFMILPLYSSIEKIDGRLLEAAKDLGANAFQRFVRVTIPLTMPGIVAGCLLVLLPAMGMFYVADLLGGGKTPLIG
NIIKSLFLNTNQFALGSAVSIALTVLMALMLYVYYRANKLLNKKVELE
>Mature_288_residues
MKIANNKFQAGTVSVIFGWLLLFVLVPNVLVLLISFLTENRQSAYFVDFIFSLDAYTQLFNDTYATVLWNSFKMSAIATF
FCLIIGYPFAFIIAKMPAKIRPILLFLVVLPFWTNSLIRIYGIKIFLGVKGVLNEALLAIGLIDEPLRLLNSELAIIIGL
VYILLPFMILPLYSSIEKIDGRLLEAAKDLGANAFQRFVRVTIPLTMPGIVAGCLLVLLPAMGMFYVADLLGGGKTPLIG
NIIKSLFLNTNQFALGSAVSIALTVLMALMLYVYYRANKLLNKKVELE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=276, Percent_Identity=61.231884057971, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI1787080, Length=229, Percent_Identity=41.4847161572052, Blast_Score=177, Evalue=5e-46,
Organism=Escherichia coli, GI1787713, Length=227, Percent_Identity=29.9559471365639, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32006; Mature: 32006

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]