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The map label for this gene is rho [H]

Identifier: 126207735

GI number: 126207735

Start: 267745

End: 269007

Strand: Reverse

Name: rho [H]

Synonym: APL_0247

Alternate gene names: 126207735

Gene position: 269007-267745 (Counterclockwise)

Preceding gene: 126207736

Following gene: 126207725

Centisome position: 11.83

GC content: 44.26

Gene sequence:

>1263_bases
ATGCATCTTACTCAATTAAAAAACACTCCTGTATCGGATCTTGTTGCGCTCGGTGAAGGTCAAATGGGCTTAGAAAATTT
AGCTCGTTTACGTAAACAAGATATTATTTTCGCTATTCTCAAGCAACACGCTAAAAGCGGTGAAGATATTTTCGGTCAAG
GTATCTTAGAGATCTTGCCGGACGGCTTCGGTTTCTTACGTTCGGCTGACAGTTCATATCTTGCCGGCCCTGACGATATT
TATGTTTCGCCAAGCCAAATTCGTCGTTTCAACTTACAAACCGGTGATAAAATCGAAGGTAAAATTCGTCCGCCGAAAGA
AGGCGAACGCTATTTCGCGCTATTAAAAGTTGATCTCGTCAATGATGACAAACCTGAAGTTTCTCGCAGCAAAATCTTAT
TTGAAAACTTAACCCCTTTACACGCCAATTCACGTTTACGTATGGAACGCGGAAACGGCTCAACCGAAGATTTAACCGCT
CGTATTCTTGATTTAGCAGCCCCTATCGGTAAAGGTCAGCGTGGTCTGATCGTTGCACCGCCAAAAGCAGGTAAAACCGT
GCTTCTGCAAAATATCGCACAAAGCATTACCCATAATCACCCCGAATGTGAATTGATCGTGTTACTGATTGACGAACGTC
CGGAAGAGGTAACGGAAATGCAACGTACCGTACGCGGTGAAGTGATTGCTTCCACCTTTGACGAGCCGGCGACACGTCAC
GTACAAGTTGCGGAAATGGTAATCGAAAAAGCGAAACGTTCGGTAGAACATAAAAAAGACGTGGTTATTCTATTAGACTC
AATCACTCGTCTTGCCCGCGCTTACAATACCGTGACGCCTGTTTCCGGTAAAATCCTTTCCGGTGGTGTGGATGCAAACG
CATTACATCGCCCGAAACGTTTCTTCGGTGCGGCACGTAACGTAGAAGAAGGCGGTAGCTTAACCATTATCGCGACGGCA
TTAGTTGATACCGGTTCGAAAATGGATGAAGTTATCTTTGAAGAGTTTAAAGGTACCGGTAATATGGAATTACACCTTTC
TCGTAAAATTGCGGAACGCCGCGTATTCCCTGCGATTGAGTTTAACCGTTCGGGTACACGTAAAGATGACTTATTAATGT
CGCCGGAAGAGCATCGTAATGCGTGGATGTTACGTAAAGTACTGAATCCGATGGATGAAGTAGCGGCAATGGAATGGTTA
ATCGATAAACTCGGCGTAGCCAAAACCAACGAAGAATTCTTCGAATTAATGAAACGTTCATAA

Upstream 100 bases:

>100_bases
ATAATTTTGCTGTATTATTCGCCACGTCTTTTTACGGTATTATCCGTATCTATTTATTCTTAAATTAATCCAATCTTACA
ATTATCAAGAATTATTTCTT

Downstream 100 bases:

>100_bases
AAAATAAAGGCACTCTGTTGAGTGCCTTTATTATATCTATTCCTTCGCTAGAATTTAGCTTCTACACTTAGTACATAGTT
TCTGCCCGGTGCGGCATAAC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MHLTQLKNTPVSDLVALGEGQMGLENLARLRKQDIIFAILKQHAKSGEDIFGQGILEILPDGFGFLRSADSSYLAGPDDI
YVSPSQIRRFNLQTGDKIEGKIRPPKEGERYFALLKVDLVNDDKPEVSRSKILFENLTPLHANSRLRMERGNGSTEDLTA
RILDLAAPIGKGQRGLIVAPPKAGKTVLLQNIAQSITHNHPECELIVLLIDERPEEVTEMQRTVRGEVIASTFDEPATRH
VQVAEMVIEKAKRSVEHKKDVVILLDSITRLARAYNTVTPVSGKILSGGVDANALHRPKRFFGAARNVEEGGSLTIIATA
LVDTGSKMDEVIFEEFKGTGNMELHLSRKIAERRVFPAIEFNRSGTRKDDLLMSPEEHRNAWMLRKVLNPMDEVAAMEWL
IDKLGVAKTNEEFFELMKRS

Sequences:

>Translated_420_residues
MHLTQLKNTPVSDLVALGEGQMGLENLARLRKQDIIFAILKQHAKSGEDIFGQGILEILPDGFGFLRSADSSYLAGPDDI
YVSPSQIRRFNLQTGDKIEGKIRPPKEGERYFALLKVDLVNDDKPEVSRSKILFENLTPLHANSRLRMERGNGSTEDLTA
RILDLAAPIGKGQRGLIVAPPKAGKTVLLQNIAQSITHNHPECELIVLLIDERPEEVTEMQRTVRGEVIASTFDEPATRH
VQVAEMVIEKAKRSVEHKKDVVILLDSITRLARAYNTVTPVSGKILSGGVDANALHRPKRFFGAARNVEEGGSLTIIATA
LVDTGSKMDEVIFEEFKGTGNMELHLSRKIAERRVFPAIEFNRSGTRKDDLLMSPEEHRNAWMLRKVLNPMDEVAAMEWL
IDKLGVAKTNEEFFELMKRS
>Mature_420_residues
MHLTQLKNTPVSDLVALGEGQMGLENLARLRKQDIIFAILKQHAKSGEDIFGQGILEILPDGFGFLRSADSSYLAGPDDI
YVSPSQIRRFNLQTGDKIEGKIRPPKEGERYFALLKVDLVNDDKPEVSRSKILFENLTPLHANSRLRMERGNGSTEDLTA
RILDLAAPIGKGQRGLIVAPPKAGKTVLLQNIAQSITHNHPECELIVLLIDERPEEVTEMQRTVRGEVIASTFDEPATRH
VQVAEMVIEKAKRSVEHKKDVVILLDSITRLARAYNTVTPVSGKILSGGVDANALHRPKRFFGAARNVEEGGSLTIIATA
LVDTGSKMDEVIFEEFKGTGNMELHLSRKIAERRVFPAIEFNRSGTRKDDLLMSPEEHRNAWMLRKVLNPMDEVAAMEWL
IDKLGVAKTNEEFFELMKRS

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=420, Percent_Identity=83.8095238095238, Blast_Score=734, Evalue=0.0,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 46874; Mature: 46874

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLTQLKNTPVSDLVALGEGQMGLENLARLRKQDIIFAILKQHAKSGEDIFGQGILEILP
CCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHCC
DGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLQTGDKIEGKIRPPKEGERYFALLKVDLV
CCHHHHHCCCCCCCCCCCCEEECHHHHHEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEC
NDDKPEVSRSKILFENLTPLHANSRLRMERGNGSTEDLTARILDLAAPIGKGQRGLIVAP
CCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEC
PKAGKTVLLQNIAQSITHNHPECELIVLLIDERPEEVTEMQRTVRGEVIASTFDEPATRH
CCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
VQVAEMVIEKAKRSVEHKKDVVILLDSITRLARAYNTVTPVSGKILSGGVDANALHRPKR
HHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHCCHH
FFGAARNVEEGGSLTIIATALVDTGSKMDEVIFEEFKGTGNMELHLSRKIAERRVFPAIE
HHHHHCCCCCCCCEEEEEEEEHHCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCCEE
FNRSGTRKDDLLMSPEEHRNAWMLRKVLNPMDEVAAMEWLIDKLGVAKTNEEFFELMKRS
ECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MHLTQLKNTPVSDLVALGEGQMGLENLARLRKQDIIFAILKQHAKSGEDIFGQGILEILP
CCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHCC
DGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLQTGDKIEGKIRPPKEGERYFALLKVDLV
CCHHHHHCCCCCCCCCCCCEEECHHHHHEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEC
NDDKPEVSRSKILFENLTPLHANSRLRMERGNGSTEDLTARILDLAAPIGKGQRGLIVAP
CCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEC
PKAGKTVLLQNIAQSITHNHPECELIVLLIDERPEEVTEMQRTVRGEVIASTFDEPATRH
CCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
VQVAEMVIEKAKRSVEHKKDVVILLDSITRLARAYNTVTPVSGKILSGGVDANALHRPKR
HHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHCCHH
FFGAARNVEEGGSLTIIATALVDTGSKMDEVIFEEFKGTGNMELHLSRKIAERRVFPAIE
HHHHHCCCCCCCCEEEEEEEEHHCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCCEE
FNRSGTRKDDLLMSPEEHRNAWMLRKVLNPMDEVAAMEWLIDKLGVAKTNEEFFELMKRS
ECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]