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The map label for this gene is aroK [H]

Identifier: 126207682

GI number: 126207682

Start: 213831

End: 214352

Strand: Reverse

Name: aroK [H]

Synonym: APL_0194

Alternate gene names: 126207682

Gene position: 214352-213831 (Counterclockwise)

Preceding gene: 126207683

Following gene: 126207681

Centisome position: 9.42

GC content: 44.06

Gene sequence:

>522_bases
ATGGCAGAAAAACGTAATATTTTCCTTATAGGTCCTATGGGTGCGGGTAAAAGCACCATTGGTCGTCAGCTCGCTCAAAT
GCTAGGAATGGAATTTATTGATTCTGACGCAGTAATTGAAGAACGTGCCGGTGCAGATATCGATTGGATTTTTGACCTTG
AAGGTGAAACCGGTTTTCGTAAGCGTGAAGAACGTATTATTAACGAGCTTACGCAAAACCAAGGTGTTGTATTATCAACC
GGCGGCGGTTCGGTGTTATCCAAAGAAAATCGCAACGTCTTGTCCGCTCGCGGTATCGTTATCTACCTTGAAACTACGGT
GGATAAACAATTCGAGAGAACGCAACGCGATAAAAAACGTCCGTTGCTGCAAACGGAGAATCCTCGTGAAACGCTGGAAG
CACTGGCAAAAGTACGCAATCCGCTCTATGAAGAAATTGCGGACATTACGCTACAAACCGATGAGCAAAGCGCTAAACTG
GTTGCGACACACATCATTGAACTGATTGATAATCTTCAATAA

Upstream 100 bases:

>100_bases
CTTGCCGTTGCTAAAAACACGAAGCAATTGAGCATTAGCGGTCGTTTTTTTCCACAAATTGAATTTGTCACATAATATTG
AAATAAAAGAAGAACAAACG

Downstream 100 bases:

>100_bases
GTTACCCGGGATTAAACGCCCCTATAAATATAATTCGCACATCATTAAATATGTCGAGATGAGGAAGTAAAATATGTTGC
AAGTCAATGTCGAATTAAAA

Product: shikimate kinase

Products: NA

Alternate protein names: SK [H]

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MAEKRNIFLIGPMGAGKSTIGRQLAQMLGMEFIDSDAVIEERAGADIDWIFDLEGETGFRKREERIINELTQNQGVVLST
GGGSVLSKENRNVLSARGIVIYLETTVDKQFERTQRDKKRPLLQTENPRETLEALAKVRNPLYEEIADITLQTDEQSAKL
VATHIIELIDNLQ

Sequences:

>Translated_173_residues
MAEKRNIFLIGPMGAGKSTIGRQLAQMLGMEFIDSDAVIEERAGADIDWIFDLEGETGFRKREERIINELTQNQGVVLST
GGGSVLSKENRNVLSARGIVIYLETTVDKQFERTQRDKKRPLLQTENPRETLEALAKVRNPLYEEIADITLQTDEQSAKL
VATHIIELIDNLQ
>Mature_172_residues
AEKRNIFLIGPMGAGKSTIGRQLAQMLGMEFIDSDAVIEERAGADIDWIFDLEGETGFRKREERIINELTQNQGVVLSTG
GGSVLSKENRNVLSARGIVIYLETTVDKQFERTQRDKKRPLLQTENPRETLEALAKVRNPLYEEIADITLQTDEQSAKLV
ATHIIELIDNLQ

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate [H]

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family [H]

Homologues:

Organism=Escherichia coli, GI87082255, Length=172, Percent_Identity=72.0930232558139, Blast_Score=260, Evalue=3e-71,
Organism=Escherichia coli, GI1786587, Length=159, Percent_Identity=31.4465408805031, Blast_Score=86, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320332, Length=126, Percent_Identity=35.7142857142857, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000623
- InterPro:   IPR023000 [H]

Pfam domain/function: PF01202 SKI [H]

EC number: =2.7.1.71 [H]

Molecular weight: Translated: 19456; Mature: 19325

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKRNIFLIGPMGAGKSTIGRQLAQMLGMEFIDSDAVIEERAGADIDWIFDLEGETGFR
CCCCCCEEEEECCCCCHHHHHHHHHHHHCHHHCCCCHHHHHHCCCCEEEEEEECCCCCHH
KREERIINELTQNQGVVLSTGGGSVLSKENRNVLSARGIVIYLETTVDKQFERTQRDKKR
HHHHHHHHHHHCCCCEEEECCCCCEECCCCCCEEECCCEEEEEECCHHHHHHHHHHHHHC
PLLQTENPRETLEALAKVRNPLYEEIADITLQTDEQSAKLVATHIIELIDNLQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCEECCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEKRNIFLIGPMGAGKSTIGRQLAQMLGMEFIDSDAVIEERAGADIDWIFDLEGETGFR
CCCCCEEEEECCCCCHHHHHHHHHHHHCHHHCCCCHHHHHHCCCCEEEEEEECCCCCHH
KREERIINELTQNQGVVLSTGGGSVLSKENRNVLSARGIVIYLETTVDKQFERTQRDKKR
HHHHHHHHHHHCCCCEEEECCCCCEECCCCCCEEECCCEEEEEECCHHHHHHHHHHHHHC
PLLQTENPRETLEALAKVRNPLYEEIADITLQTDEQSAKLVATHIIELIDNLQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCEECCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA