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The map label for this gene is pykA [H]

Identifier: 126207675

GI number: 126207675

Start: 206772

End: 208211

Strand: Reverse

Name: pykA [H]

Synonym: APL_0187

Alternate gene names: 126207675

Gene position: 208211-206772 (Counterclockwise)

Preceding gene: 126207679

Following gene: 126207674

Centisome position: 9.15

GC content: 43.96

Gene sequence:

>1440_bases
ATGTCTAGAAAACTCAGAAGAACAAAAATTGTTTGTACAATGGGTCCAGCTACCGATCGTGGTAATAATTTAGAAAAAGT
TATCGCTGCAGGTGCGAATATGGTTCGTATGAACTTCTCGCACGGTGTGCCGGAAGATCACATCGAACGTGCAAATAAAG
TGCGTGAAATTGCAGCTAGATTAGGTAAAACCGTAGCGATTTTAGGCGATTTACAGGGGCCTAAAATTCGTGTTTCTACA
TTCAAAGACGGTAAAATTTTCTTAAATATCGGTGACAAATTCACTTTAGATGCGGATTTACCGCGTGGTGAAGGTCATCA
AGATGCGGTCGGCTTAGACTATAAAAACTTACCGAACGATGTCGTACCGGGTGATATTCTTTTATTAGATGACGGTAACG
TACAATTAAAAGTTCTTTCGGTTGAAGGCGTGAAAGTGCATACGGAAGTGACTGTCGGTGGCCCGTTATCGAATAACAAA
GGTATCAATAAGTTAGGCGGCGGTTTATCTGCACCGGCATTAACGGAAAAAGATAAAGAAGACATTAAACTTGCGGCTAA
AATCGGTGTAGATTACTTAGCGGTATCATTCCCTCAATCAAGTGCGGATTTAGACTATGCGCGTCAATTGGCACGTGAAG
CGGGATTAGAAGCGAAAATCGTGGCGAAAGTGGAACGTGCGGAAACCGTTGCAACCGAAGAAGCGATGGATGACATTATT
TTAGGTTCTGACGTGGTAATGGTTGCACGTGGCGACTTAGGTGTTGAAATCGGTGATGCGGCGTTAGTCGGCGTACAAAA
ACGTTTAATTCGTCGTGCTCGTAAATTAAACCGCGTAGTCATTACCGCTACCCAAATGATGGAATCGATGATCAAAAAAC
CGATGCCGACACGTGCCGAAGTTATGGACGTAGCGAATGCGGTATTAGACGGTACGGATGCGGTAATGCTTTCCGGTGAA
ACGGCAAACGGCGATTATCCGGTTGAAACGGTTAAATCAATGGCGGAAGTCTGTTTAGGTGCGGAAACGATGCCGAGCAT
CAACATCTCTCGTCACCGTATGGAAGGTACATTCGCAACGATTGATGAAGCGGTTGCTATGTCTGCGATGTACACCGCTA
ACCACTTGGAAGGCGTATCTGCGCTTATCGCTTTAACCCACTCGGGCGAAACGGCGAAATTAATGAGTCGTATCAGTTCA
GGTTTACCGATTTATGCGTTATCTCGTAATCAACAAGCATTAAATCGTACCGCGCTTTATCGCGGCGTAACACCGGTATT
CTATGATGAAGAAAGCCGTACTTTCGAAGGTGCGAAAAAAGCTATCGCTCTATTAAAAGAGAAAGGCTATTTAATGTCCG
GCGATTTAGTATTATTAACTCAAGGTGACGAGTTAAAAGAAGGCGGTACGAACACCAGCCGTTTATTAAAAGTTGAATAA

Upstream 100 bases:

>100_bases
ACGATTTAAGCGTATAATACTCTGCGTTTAAGGCGTTAAAATACCGCCGATGAAAAGAATTTTCAATTTCTAAATAATCA
ATAATTACGGAGTCTAATCA

Downstream 100 bases:

>100_bases
GCACTTTTTAATCAGACCGAACCCACGCAAAAGCGTGGGTTTTTACTATTCGATATCAATGAATTACTTTAAAGATCGCA
AATTCTAATGAAAAAACAAC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSRKLRRTKIVCTMGPATDRGNNLEKVIAAGANMVRMNFSHGVPEDHIERANKVREIAARLGKTVAILGDLQGPKIRVST
FKDGKIFLNIGDKFTLDADLPRGEGHQDAVGLDYKNLPNDVVPGDILLLDDGNVQLKVLSVEGVKVHTEVTVGGPLSNNK
GINKLGGGLSAPALTEKDKEDIKLAAKIGVDYLAVSFPQSSADLDYARQLAREAGLEAKIVAKVERAETVATEEAMDDII
LGSDVVMVARGDLGVEIGDAALVGVQKRLIRRARKLNRVVITATQMMESMIKKPMPTRAEVMDVANAVLDGTDAVMLSGE
TANGDYPVETVKSMAEVCLGAETMPSINISRHRMEGTFATIDEAVAMSAMYTANHLEGVSALIALTHSGETAKLMSRISS
GLPIYALSRNQQALNRTALYRGVTPVFYDEESRTFEGAKKAIALLKEKGYLMSGDLVLLTQGDELKEGGTNTSRLLKVE

Sequences:

>Translated_479_residues
MSRKLRRTKIVCTMGPATDRGNNLEKVIAAGANMVRMNFSHGVPEDHIERANKVREIAARLGKTVAILGDLQGPKIRVST
FKDGKIFLNIGDKFTLDADLPRGEGHQDAVGLDYKNLPNDVVPGDILLLDDGNVQLKVLSVEGVKVHTEVTVGGPLSNNK
GINKLGGGLSAPALTEKDKEDIKLAAKIGVDYLAVSFPQSSADLDYARQLAREAGLEAKIVAKVERAETVATEEAMDDII
LGSDVVMVARGDLGVEIGDAALVGVQKRLIRRARKLNRVVITATQMMESMIKKPMPTRAEVMDVANAVLDGTDAVMLSGE
TANGDYPVETVKSMAEVCLGAETMPSINISRHRMEGTFATIDEAVAMSAMYTANHLEGVSALIALTHSGETAKLMSRISS
GLPIYALSRNQQALNRTALYRGVTPVFYDEESRTFEGAKKAIALLKEKGYLMSGDLVLLTQGDELKEGGTNTSRLLKVE
>Mature_478_residues
SRKLRRTKIVCTMGPATDRGNNLEKVIAAGANMVRMNFSHGVPEDHIERANKVREIAARLGKTVAILGDLQGPKIRVSTF
KDGKIFLNIGDKFTLDADLPRGEGHQDAVGLDYKNLPNDVVPGDILLLDDGNVQLKVLSVEGVKVHTEVTVGGPLSNNKG
INKLGGGLSAPALTEKDKEDIKLAAKIGVDYLAVSFPQSSADLDYARQLAREAGLEAKIVAKVERAETVATEEAMDDIIL
GSDVVMVARGDLGVEIGDAALVGVQKRLIRRARKLNRVVITATQMMESMIKKPMPTRAEVMDVANAVLDGTDAVMLSGET
ANGDYPVETVKSMAEVCLGAETMPSINISRHRMEGTFATIDEAVAMSAMYTANHLEGVSALIALTHSGETAKLMSRISSG
LPIYALSRNQQALNRTALYRGVTPVFYDEESRTFEGAKKAIALLKEKGYLMSGDLVLLTQGDELKEGGTNTSRLLKVE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=494, Percent_Identity=37.246963562753, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI33286420, Length=494, Percent_Identity=37.246963562753, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI33286418, Length=494, Percent_Identity=36.8421052631579, Blast_Score=266, Evalue=4e-71,
Organism=Homo sapiens, GI10835121, Length=498, Percent_Identity=35.5421686746988, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI32967597, Length=498, Percent_Identity=35.5421686746988, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=35.7388316151203, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=35.7388316151203, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=34.5991561181435, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=34.5991561181435, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=33.7962962962963, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=74.5833333333333, Blast_Score=724, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=475, Percent_Identity=38.1052631578947, Blast_Score=293, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17544584, Length=490, Percent_Identity=38.1632653061225, Blast_Score=285, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI17506831, Length=493, Percent_Identity=37.1196754563895, Blast_Score=247, Evalue=9e-66,
Organism=Caenorhabditis elegans, GI71984406, Length=493, Percent_Identity=37.1196754563895, Blast_Score=247, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17506829, Length=493, Percent_Identity=37.1196754563895, Blast_Score=247, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI71984413, Length=493, Percent_Identity=37.1196754563895, Blast_Score=246, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6319279, Length=489, Percent_Identity=37.2188139059305, Blast_Score=259, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6324923, Length=490, Percent_Identity=33.8775510204082, Blast_Score=243, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24648964, Length=497, Percent_Identity=38.2293762575453, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI28571814, Length=497, Percent_Identity=38.2293762575453, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24648966, Length=411, Percent_Identity=37.956204379562, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24581235, Length=493, Percent_Identity=31.8458417849899, Blast_Score=219, Evalue=5e-57,
Organism=Drosophila melanogaster, GI24646914, Length=283, Percent_Identity=34.982332155477, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51513; Mature: 51382

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKLRRTKIVCTMGPATDRGNNLEKVIAAGANMVRMNFSHGVPEDHIERANKVREIAAR
CCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
LGKTVAILGDLQGPKIRVSTFKDGKIFLNIGDKFTLDADLPRGEGHQDAVGLDYKNLPND
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCCCCCCHHCCCCC
VVPGDILLLDDGNVQLKVLSVEGVKVHTEVTVGGPLSNNKGINKLGGGLSAPALTEKDKE
CCCCCEEEEECCCEEEEEEEECCEEEEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCHH
DIKLAAKIGVDYLAVSFPQSSADLDYARQLAREAGLEAKIVAKVERAETVATEEAMDDII
HHEEHHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHE
LGSDVVMVARGDLGVEIGDAALVGVQKRLIRRARKLNRVVITATQMMESMIKKPMPTRAE
ECCCEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHCCCCCHHH
VMDVANAVLDGTDAVMLSGETANGDYPVETVKSMAEVCLGAETMPSINISRHRMEGTFAT
HHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCCHHHH
IDEAVAMSAMYTANHLEGVSALIALTHSGETAKLMSRISSGLPIYALSRNQQALNRTALY
HHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHH
RGVTPVFYDEESRTFEGAKKAIALLKEKGYLMSGDLVLLTQGDELKEGGTNTSRLLKVE
HCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEECCEEEEECCCHHHCCCCCCCEEEEEC
>Mature Secondary Structure 
SRKLRRTKIVCTMGPATDRGNNLEKVIAAGANMVRMNFSHGVPEDHIERANKVREIAAR
CCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
LGKTVAILGDLQGPKIRVSTFKDGKIFLNIGDKFTLDADLPRGEGHQDAVGLDYKNLPND
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCCCCCCHHCCCCC
VVPGDILLLDDGNVQLKVLSVEGVKVHTEVTVGGPLSNNKGINKLGGGLSAPALTEKDKE
CCCCCEEEEECCCEEEEEEEECCEEEEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCHH
DIKLAAKIGVDYLAVSFPQSSADLDYARQLAREAGLEAKIVAKVERAETVATEEAMDDII
HHEEHHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHE
LGSDVVMVARGDLGVEIGDAALVGVQKRLIRRARKLNRVVITATQMMESMIKKPMPTRAE
ECCCEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHCCCCCHHH
VMDVANAVLDGTDAVMLSGETANGDYPVETVKSMAEVCLGAETMPSINISRHRMEGTFAT
HHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCCHHHH
IDEAVAMSAMYTANHLEGVSALIALTHSGETAKLMSRISSGLPIYALSRNQQALNRTALY
HHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHH
RGVTPVFYDEESRTFEGAKKAIALLKEKGYLMSGDLVLLTQGDELKEGGTNTSRLLKVE
HCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEECCEEEEECCCHHHCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]