Click here to switch to the map view.

The map label for this gene is leuC [H]

Identifier: 126207627

GI number: 126207627

Start: 154174

End: 155583

Strand: Reverse

Name: leuC [H]

Synonym: APL_0139

Alternate gene names: 126207627

Gene position: 155583-154174 (Counterclockwise)

Preceding gene: 126207632

Following gene: 126207626

Centisome position: 6.84

GC content: 47.59

Gene sequence:

>1410_bases
ATGGCAAAAACATTATACGAAAAACTATTTGATGCCCACGTTGTATATGAAGCCGCCGGCGAAACGCCGATTCTTTATAT
CAACCGCCATTTAATTCATGAAGTGACAAGCCCGCAAGCGTTTGACGGTTTACGTGTCGCCGGTCGTCAAGTACGCCAAA
TCGGTAAAACGTTCGGTACAATGGATCACAGCATCTCAACCCAAGTGCGTGACGTCAATAAATTAGAAGGTCAGGCGAAA
ATCCAAGTTTTAGAATTAGCGAAAAACTGTGAAGCGAACGGGATTTCGTTATTCGATATGCAAACCAAAGAACAAGGTAT
CGTACACGTTATGGGGCCGGAGCAAGGTTTAACCCTACCGGGTATGACGATTGTCTGCGGTGACTCACACACCGCAACAC
ACGGTGCTTTCGGTGCGCTTGCGTTTGGTATCGGTACATCGGAAGTGGAACACGTGCTTGCAACGCAAACCTTAAAACAA
GCGCGTGCGAAAAGTATGAAAGTGGAAGTTCGCGGCAAAGTGAACCCGGGCATTACCGCCAAAGATATCGTGCTGGCAAT
CATCGGTAAAACAACTATGGCGGGCGGTACCGGTCATGTGGTGGAATTCTGCGGTGAAGCGATTCGTAATTTATCGATGG
AAGGCCGTATGACGGTATGTAATATGGCGATCGAATTCGGCGCTAAAGCCGGTTTAGTCGCACCGGACGAAACCACCTTC
GCTTACTTAAAAGATCGTCCGCACGCACCGAAAGGCAAAGATTGGGACGATGCGGTCGAATATTGGACAACCTTAAAATC
AGACGATGATGCGGTCTTTGATTCAGTCGTTGTATTAGAAGCGAAAGATATTGCGCCGCAAGTCACTTGGGGAACCAATC
CGGGACAAGTCATCGGTATTGACCAAGTGGTACCGAATCCACAAGAAATGGCGGATCCGGTCACTAAAGCTTCAGCGGAA
AAAGCGCTCGCATATATCGGCTTAGATGCGAATACCGATATGAAAAACATCCCTGTGGATCAAGTATTTATCGGCTCTTG
TACTAACTCTCGTATTGAAGACTTACGTGCGGCGGCGGCAGTAATGAAAGGTCGTAAAAAAGCGGATAACGTAAAACGTG
TGCTAGTTGTACCGGGTTCGGGTTTGGTAAAAGAACAGGCGGAAAAAGAAGGCTTAGATAAGATCTTTATCGAGGCTGGC
GCAGAATGGCGTAATCCGGGCTGTTCAATGTGTTTAGGTATGAATGATGACCGTTTAGGCGAATGGGAACGTTGTGCTTC
AACCTCTAACCGTAACTTTGAAGGTCGCCAAGGTCGTAACGGTCGTACCCACTTGGTTAGCCCGGCAATGGCGGCAGCGG
CGGCAATGTTCGGTAAATTTGTCGATATTCGTCACGTGGAATTAAATTAA

Upstream 100 bases:

>100_bases
GTTATCAACATTACACAAGCGGTCATTTTTACAAAAAATTTTGCAAATTTCACCGCCTATTATATGCATTCACAATATCC
TTTCAGAGTTGAACAATATT

Downstream 100 bases:

>100_bases
GGATAAAAAAATGGCAGGATTAAAACAACACTCAGGTTTAGTCGTTCCGCTTGATGCAGCGAATGTGGATACTGACGCAA
TCATTCCAAAACAGTTCTTA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MAKTLYEKLFDAHVVYEAAGETPILYINRHLIHEVTSPQAFDGLRVAGRQVRQIGKTFGTMDHSISTQVRDVNKLEGQAK
IQVLELAKNCEANGISLFDMQTKEQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
ARAKSMKVEVRGKVNPGITAKDIVLAIIGKTTMAGGTGHVVEFCGEAIRNLSMEGRMTVCNMAIEFGAKAGLVAPDETTF
AYLKDRPHAPKGKDWDDAVEYWTTLKSDDDAVFDSVVVLEAKDIAPQVTWGTNPGQVIGIDQVVPNPQEMADPVTKASAE
KALAYIGLDANTDMKNIPVDQVFIGSCTNSRIEDLRAAAAVMKGRKKADNVKRVLVVPGSGLVKEQAEKEGLDKIFIEAG
AEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSPAMAAAAAMFGKFVDIRHVELN

Sequences:

>Translated_469_residues
MAKTLYEKLFDAHVVYEAAGETPILYINRHLIHEVTSPQAFDGLRVAGRQVRQIGKTFGTMDHSISTQVRDVNKLEGQAK
IQVLELAKNCEANGISLFDMQTKEQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
ARAKSMKVEVRGKVNPGITAKDIVLAIIGKTTMAGGTGHVVEFCGEAIRNLSMEGRMTVCNMAIEFGAKAGLVAPDETTF
AYLKDRPHAPKGKDWDDAVEYWTTLKSDDDAVFDSVVVLEAKDIAPQVTWGTNPGQVIGIDQVVPNPQEMADPVTKASAE
KALAYIGLDANTDMKNIPVDQVFIGSCTNSRIEDLRAAAAVMKGRKKADNVKRVLVVPGSGLVKEQAEKEGLDKIFIEAG
AEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSPAMAAAAAMFGKFVDIRHVELN
>Mature_468_residues
AKTLYEKLFDAHVVYEAAGETPILYINRHLIHEVTSPQAFDGLRVAGRQVRQIGKTFGTMDHSISTQVRDVNKLEGQAKI
QVLELAKNCEANGISLFDMQTKEQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQA
RAKSMKVEVRGKVNPGITAKDIVLAIIGKTTMAGGTGHVVEFCGEAIRNLSMEGRMTVCNMAIEFGAKAGLVAPDETTFA
YLKDRPHAPKGKDWDDAVEYWTTLKSDDDAVFDSVVVLEAKDIAPQVTWGTNPGQVIGIDQVVPNPQEMADPVTKASAEK
ALAYIGLDANTDMKNIPVDQVFIGSCTNSRIEDLRAAAAVMKGRKKADNVKRVLVVPGSGLVKEQAEKEGLDKIFIEAGA
EWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSPAMAAAAAMFGKFVDIRHVELN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=365, Percent_Identity=26.8493150684932, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI8659555, Length=453, Percent_Identity=26.7108167770419, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI41352693, Length=392, Percent_Identity=25, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786259, Length=467, Percent_Identity=74.9464668094218, Blast_Score=716, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=363, Percent_Identity=26.7217630853994, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI87081781, Length=321, Percent_Identity=26.791277258567, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367097, Length=354, Percent_Identity=25.7062146892655, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=363, Percent_Identity=29.2011019283747, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI32564738, Length=363, Percent_Identity=28.9256198347107, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=304, Percent_Identity=29.2763157894737, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17568399, Length=458, Percent_Identity=26.4192139737991, Blast_Score=105, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=60.5095541401274, Blast_Score=583, Evalue=1e-167,
Organism=Saccharomyces cerevisiae, GI6323335, Length=373, Percent_Identity=31.3672922252011, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=371, Percent_Identity=29.9191374663073, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320440, Length=443, Percent_Identity=25.5079006772009, Blast_Score=119, Evalue=8e-28,
Organism=Drosophila melanogaster, GI281365315, Length=412, Percent_Identity=25.7281553398058, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17864292, Length=412, Percent_Identity=25.7281553398058, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI161076999, Length=412, Percent_Identity=25.7281553398058, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28571643, Length=364, Percent_Identity=26.0989010989011, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24645686, Length=460, Percent_Identity=26.304347826087, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17137564, Length=374, Percent_Identity=27.807486631016, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50706; Mature: 50574

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLYEKLFDAHVVYEAAGETPILYINRHLIHEVTSPQAFDGLRVAGRQVRQIGKTFGT
CCHHHHHHHHHHHEEEECCCCCCEEEECHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
MDHSISTQVRDVNKLEGQAKIQVLELAKNCEANGISLFDMQTKEQGIVHVMGPEQGLTLP
CCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQARAKSMKVEVRGKVNPGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCH
KDIVLAIIGKTTMAGGTGHVVEFCGEAIRNLSMEGRMTVCNMAIEFGAKAGLVAPDETTF
HHHHEEEECCHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEE
AYLKDRPHAPKGKDWDDAVEYWTTLKSDDDAVFDSVVVLEAKDIAPQVTWGTNPGQVIGI
EEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHCEEEEEEECCCCCEEEECCCCCCEEEE
DQVVPNPQEMADPVTKASAEKALAYIGLDANTDMKNIPVDQVFIGSCTNSRIEDLRAAAA
CCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
VMKGRKKADNVKRVLVVPGSGLVKEQAEKEGLDKIFIEAGAEWRNPGCSMCLGMNDDRLG
HHHCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCCCCC
EWERCASTSNRNFEGRQGRNGRTHLVSPAMAAAAAMFGKFVDIRHVELN
HHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHEEEEEEEC
>Mature Secondary Structure 
AKTLYEKLFDAHVVYEAAGETPILYINRHLIHEVTSPQAFDGLRVAGRQVRQIGKTFGT
CHHHHHHHHHHHEEEECCCCCCEEEECHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
MDHSISTQVRDVNKLEGQAKIQVLELAKNCEANGISLFDMQTKEQGIVHVMGPEQGLTLP
CCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQARAKSMKVEVRGKVNPGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCH
KDIVLAIIGKTTMAGGTGHVVEFCGEAIRNLSMEGRMTVCNMAIEFGAKAGLVAPDETTF
HHHHEEEECCHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEE
AYLKDRPHAPKGKDWDDAVEYWTTLKSDDDAVFDSVVVLEAKDIAPQVTWGTNPGQVIGI
EEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHCEEEEEEECCCCCEEEECCCCCCEEEE
DQVVPNPQEMADPVTKASAEKALAYIGLDANTDMKNIPVDQVFIGSCTNSRIEDLRAAAA
CCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
VMKGRKKADNVKRVLVVPGSGLVKEQAEKEGLDKIFIEAGAEWRNPGCSMCLGMNDDRLG
HHHCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCCCCC
EWERCASTSNRNFEGRQGRNGRTHLVSPAMAAAAAMFGKFVDIRHVELN
HHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA