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The map label for this gene is thyA [H]

Identifier: 126207603

GI number: 126207603

Start: 132285

End: 133136

Strand: Reverse

Name: thyA [H]

Synonym: APL_0115

Alternate gene names: 126207603

Gene position: 133136-132285 (Counterclockwise)

Preceding gene: 126207606

Following gene: 126207598

Centisome position: 5.85

GC content: 42.37

Gene sequence:

>852_bases
ATGAAACAGTATTTAGATTTATGCCATCGTATCGTAAACGAAGGAAAATGGGTAGAGAACGAACGTACTGGTAAGCGTTG
TTTAACCGTAATTAATGCCGATTTAACCTACAATGTTGCAACCGGAGAGTTTCCGCTTGTTACAACAAGAAGAAGTTTTT
GGAAAGCGGCGATTGCGGAATTATTGGGTTATATTCGAGGCTATGATAATGCCGCCGATTTCCGCAAACTGGGAACAAAA
TCATGGGATGCCAATGCAAATCAAAACTTGGCTTGGTTAGCCAATCCGTATCGTAAAGGCGAAGATGATATGGGTTTGGT
TTACGGCGCGGTCGGACGTAACTTCCCGAAAGCGGACGGAGGTTCCGTCGATTTATTGCGCCAGATTGTCGATGATTTAA
AAAAGGGAGTGGATAATCGCGGCGAAATTTATACCTTTTATCATCCGGGCGTATTCCATATGGGCTGTTTACGTCCCTGC
TTATATAGCCATCATTTCTCGCTTTTAGACGGCACGCTTTATTTAAACAGTACGCAACGTTCCGCCGATGTACCGCTTGG
GCTCAATTGGAATATGATCCAATGCTATACCTTCTTGGCTTTAATGGCGCAAATTACCGGACATAAAGCCGGACAAGCGT
TTCATAAAATCGTAAATGCGCATATTTATGAAGACCAATTGGAATTGATGCGTGACGTACAATTGAAAAGAAAGCCGCTG
GCTTCTCCTAAACTTTGGATTAATCCGGCAATTAAATCATTAGAGGATTTAGAAACTTGGGTAACGCTCGATGATTTTAA
AGTCGAAGGTTACGAATATCATCCGGCAATCCAATATCCGTTCTCGGTATAA

Upstream 100 bases:

>100_bases
TCAGTTTTTTCATATCGCACTCCTTATCGCTTTATAAAAATTTATTATCCATTTTACCGAATAAATGGCAAAATTACCTA
AGTTTTTTTAAGAGGTCACC

Downstream 100 bases:

>100_bases
GAGAAACAAAAAAGCCTAGAATGAATCTAGGCTTTAAAAACTTTGCAAAGAATTATTTTACTAAATCTTTTAATGCTTTA
CCTGCAACAAACGCCGGCAC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKQYLDLCHRIVNEGKWVENERTGKRCLTVINADLTYNVATGEFPLVTTRRSFWKAAIAELLGYIRGYDNAADFRKLGTK
SWDANANQNLAWLANPYRKGEDDMGLVYGAVGRNFPKADGGSVDLLRQIVDDLKKGVDNRGEIYTFYHPGVFHMGCLRPC
LYSHHFSLLDGTLYLNSTQRSADVPLGLNWNMIQCYTFLALMAQITGHKAGQAFHKIVNAHIYEDQLELMRDVQLKRKPL
ASPKLWINPAIKSLEDLETWVTLDDFKVEGYEYHPAIQYPFSV

Sequences:

>Translated_283_residues
MKQYLDLCHRIVNEGKWVENERTGKRCLTVINADLTYNVATGEFPLVTTRRSFWKAAIAELLGYIRGYDNAADFRKLGTK
SWDANANQNLAWLANPYRKGEDDMGLVYGAVGRNFPKADGGSVDLLRQIVDDLKKGVDNRGEIYTFYHPGVFHMGCLRPC
LYSHHFSLLDGTLYLNSTQRSADVPLGLNWNMIQCYTFLALMAQITGHKAGQAFHKIVNAHIYEDQLELMRDVQLKRKPL
ASPKLWINPAIKSLEDLETWVTLDDFKVEGYEYHPAIQYPFSV
>Mature_283_residues
MKQYLDLCHRIVNEGKWVENERTGKRCLTVINADLTYNVATGEFPLVTTRRSFWKAAIAELLGYIRGYDNAADFRKLGTK
SWDANANQNLAWLANPYRKGEDDMGLVYGAVGRNFPKADGGSVDLLRQIVDDLKKGVDNRGEIYTFYHPGVFHMGCLRPC
LYSHHFSLLDGTLYLNSTQRSADVPLGLNWNMIQCYTFLALMAQITGHKAGQAFHKIVNAHIYEDQLELMRDVQLKRKPL
ASPKLWINPAIKSLEDLETWVTLDDFKVEGYEYHPAIQYPFSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=289, Percent_Identity=32.5259515570934, Blast_Score=149, Evalue=3e-36,
Organism=Escherichia coli, GI1789191, Length=283, Percent_Identity=31.8021201413428, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71993377, Length=290, Percent_Identity=27.5862068965517, Blast_Score=123, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6324648, Length=299, Percent_Identity=25.752508361204, Blast_Score=89, Evalue=6e-19,
Organism=Drosophila melanogaster, GI17137556, Length=290, Percent_Identity=28.9655172413793, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 32313; Mature: 32313

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLCHRIVNEGKWVENERTGKRCLTVINADLTYNVATGEFPLVTTRRSFWKAAIAE
CCHHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCEEEEEECCCCCEEECHHHHHHHHHHH
LLGYIRGYDNAADFRKLGTKSWDANANQNLAWLANPYRKGEDDMGLVYGAVGRNFPKADG
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEHHCCCCCCCCC
GSVDLLRQIVDDLKKGVDNRGEIYTFYHPGVFHMGCLRPCLYSHHFSLLDGTLYLNSTQR
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
SADVPLGLNWNMIQCYTFLALMAQITGHKAGQAFHKIVNAHIYEDQLELMRDVQLKRKPL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ASPKLWINPAIKSLEDLETWVTLDDFKVEGYEYHPAIQYPFSV
CCCCEEECHHHHHHHHHHHHEEEECEEECCEEECCCCCCCCCC
>Mature Secondary Structure
MKQYLDLCHRIVNEGKWVENERTGKRCLTVINADLTYNVATGEFPLVTTRRSFWKAAIAE
CCHHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCEEEEEECCCCCEEECHHHHHHHHHHH
LLGYIRGYDNAADFRKLGTKSWDANANQNLAWLANPYRKGEDDMGLVYGAVGRNFPKADG
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEHHCCCCCCCCC
GSVDLLRQIVDDLKKGVDNRGEIYTFYHPGVFHMGCLRPCLYSHHFSLLDGTLYLNSTQR
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
SADVPLGLNWNMIQCYTFLALMAQITGHKAGQAFHKIVNAHIYEDQLELMRDVQLKRKPL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ASPKLWINPAIKSLEDLETWVTLDDFKVEGYEYHPAIQYPFSV
CCCCEEECHHHHHHHHHHHHEEEECEEECCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA