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The map label for this gene is rlmL

Identifier: 126207548

GI number: 126207548

Start: 65092

End: 67230

Strand: Direct

Name: rlmL

Synonym: APL_0060

Alternate gene names: 126207548

Gene position: 65092-67230 (Clockwise)

Preceding gene: 126207545

Following gene: 126207549

Centisome position: 2.86

GC content: 43.2

Gene sequence:

>2139_bases
ATGACAACTCAAATAACTTATTTTGCTACAGCTGCTCGTGGCTTTGAAGAAATGTTAAAAACGGAACTGGAGCAGATCTG
CCAAGCCGAGTGTAAAGTCACACAAGGCGGAGTACATTTTACGACGACCCAACGAGGGGCATATCAAGCATTACTACACA
GCCGTTTGGCTTCTCGCATTTTATTACCGTTGGTCACCACCAAAATTTTTAGTGATCTGGATTTATACGCCACGATCGTC
GGTATTAACTGGGCGTACATTTTTGATCCTCGTGATACCTTCTTTGTGGATTTCAACGGGACGAACCGAGAGATCCGCAA
CACGCAATTCGGTGCGATGCGGGTAAAAGACGGGGTGGTCGATTATTTTGAGCGTAAAGGCTTTACTCGCCCGACAGTAG
ATAAAGATCATGCGGATATTCGAATTCACGTTTATCTCGACCGAGAAAATATGGTGGTATCGCTTGATTTAAGCGGTGAT
GCGTTACATATGCGAGGCTACCGTGAAGACACAGGTAAAGCACCGTTACGTGAAACATTGGCGGCGGCGATAGTTCTTCG
TTCCGGCTGGCAAAAAGGCACACCGTTGGTTGATCCGATGTGTGGTTCCGGTACCTTGTTGATTGAAGCTGCACAAATGC
AAGCGGGTATCGCACCGCAATTACACCGTAAACATTGGGGCTTTAATGCGTGGAAAGGGCATCAACAAGCGGTTTGGAAA
GAAGTGCTGGAACAAGCCTATTTGCAACAAAATGAAGAAATTCAACCGCTTTTCTTCGGCTTTGACCTTGATCATCGCGT
ACTGGCTAAAGCCAAACAGAATGCGAAAAATGCCGGTGTGGCACATTTAATTCAATGGCAACAAGGCGATATAGCGGCGT
TAAAAAATCCTTGCCCTGAGCAAGTCGGTACGGTGATTTGTAACCCGCCGTACGGTGAGCGTTTAGGTACGACACCGGCG
CTGATTGCACTTTATTCGGTATTCGGACAACGTTTAAAACAACAATTTAGTGGTTGGAATGCCTCGATTTTTAGTGGTGA
ACCGGAATTATTGAACTGCTTACGTTTACGTTCTCACCGTCAATTCAAAGCGAAAAACGGCCCGTTAGACTGTTTACAAA
AGAATTATCAAATTAGCGAACGCACAGCAGCAGAGCAGCAAGCTGATGAACTTAAATTTGAGCAAAATGCGCAAGTTGCC
CCCGACTTTGCGAATCGTTTAGCGAAGAATATTAAGAAAATTGAAAAATGGGCGAAGCAACAAGGTATTAATGCGTATCG
TTTATATGATGCGGATTTACCGGAATATAATTTAGCGGTCGATCGCTATGATGATCATATTGTGGTACAAGAGTATGCCG
CACCGAAAAACATTGACGAACAAAAAGCTCGCCAACGCTTACTAGATGCCGTGTCTGCAACCTTGTATGTAACGGGCGTG
GAAACCAATAAGTTGGTATTAAAAGTACGTCAAAAGCAGAAAGGTACAAACCAATATGAGAAATTAGCCAATAAAGGTGA
TTATTTCTATGTCACCGAATACGGTGCAAAATTATGGGTGAATTTGACCGATTATCTTGATACCGGCTTATTCTTAGATC
ACCGTCTCACACGTAAGATGGTAGGGCAGATGGCAAAAGGTAAAACGTTCCTTAATCTTTTTGCTTACACCGGTTCAGCG
ACTATTCACGCCGCATTAAACGGTGCGAAATCAACCACCACGGTAGATATGTCAAATACTTACCTGAATTGGGCGGAACA
AAATTTAGAGCTAAATAATTTACCACTGCGTAATAACCGCTTATTCCAAGCTGATTGCTTGCAGTGGTTGGCGGAATGTC
GAGAACGCTTTGAGTTGATTTTCGTTGATCCGCCGACGTTCTCGAATTCAAAACGAATGGAAGACAGTTGGGACGTACAG
CGTGATCATATTAAATTAATGACACAGCTTAAACGCATTTTAACGACGGACGGAACAATCGTATTCTCAAATAATAAGCG
TGGCTTTAAAATGGATTTCGAAGGTTTAGCCGAATTAGGGTTACAAGCGGAAAATATTTCGCATAAAACATTACCGCTTG
ATTTTGAGCGTAATCCGCAAATTCATAACTGTTGGATCATTCGCCACATTGAAAATTAA

Upstream 100 bases:

>100_bases
TTGTCCAGTTAAATTTCCGTTTGATTTGTAAAACTTTTCAACTATTAGACCGCTTGTTCCGCTATAATAATGTTAAATTT
TAGCTAGAAGAACGATAACA

Downstream 100 bases:

>100_bases
CAATCACGAAATACACGAAAAGTTTTGTGTATTCCGCCTATTGGATGGTAAATAAAAATGTTAGATATTGTTTTATATGA
ACCGGAAATTCCACAAAACA

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 712; Mature: 711

Protein sequence:

>712_residues
MTTQITYFATAARGFEEMLKTELEQICQAECKVTQGGVHFTTTQRGAYQALLHSRLASRILLPLVTTKIFSDLDLYATIV
GINWAYIFDPRDTFFVDFNGTNREIRNTQFGAMRVKDGVVDYFERKGFTRPTVDKDHADIRIHVYLDRENMVVSLDLSGD
ALHMRGYREDTGKAPLRETLAAAIVLRSGWQKGTPLVDPMCGSGTLLIEAAQMQAGIAPQLHRKHWGFNAWKGHQQAVWK
EVLEQAYLQQNEEIQPLFFGFDLDHRVLAKAKQNAKNAGVAHLIQWQQGDIAALKNPCPEQVGTVICNPPYGERLGTTPA
LIALYSVFGQRLKQQFSGWNASIFSGEPELLNCLRLRSHRQFKAKNGPLDCLQKNYQISERTAAEQQADELKFEQNAQVA
PDFANRLAKNIKKIEKWAKQQGINAYRLYDADLPEYNLAVDRYDDHIVVQEYAAPKNIDEQKARQRLLDAVSATLYVTGV
ETNKLVLKVRQKQKGTNQYEKLANKGDYFYVTEYGAKLWVNLTDYLDTGLFLDHRLTRKMVGQMAKGKTFLNLFAYTGSA
TIHAALNGAKSTTTVDMSNTYLNWAEQNLELNNLPLRNNRLFQADCLQWLAECRERFELIFVDPPTFSNSKRMEDSWDVQ
RDHIKLMTQLKRILTTDGTIVFSNNKRGFKMDFEGLAELGLQAENISHKTLPLDFERNPQIHNCWIIRHIEN

Sequences:

>Translated_712_residues
MTTQITYFATAARGFEEMLKTELEQICQAECKVTQGGVHFTTTQRGAYQALLHSRLASRILLPLVTTKIFSDLDLYATIV
GINWAYIFDPRDTFFVDFNGTNREIRNTQFGAMRVKDGVVDYFERKGFTRPTVDKDHADIRIHVYLDRENMVVSLDLSGD
ALHMRGYREDTGKAPLRETLAAAIVLRSGWQKGTPLVDPMCGSGTLLIEAAQMQAGIAPQLHRKHWGFNAWKGHQQAVWK
EVLEQAYLQQNEEIQPLFFGFDLDHRVLAKAKQNAKNAGVAHLIQWQQGDIAALKNPCPEQVGTVICNPPYGERLGTTPA
LIALYSVFGQRLKQQFSGWNASIFSGEPELLNCLRLRSHRQFKAKNGPLDCLQKNYQISERTAAEQQADELKFEQNAQVA
PDFANRLAKNIKKIEKWAKQQGINAYRLYDADLPEYNLAVDRYDDHIVVQEYAAPKNIDEQKARQRLLDAVSATLYVTGV
ETNKLVLKVRQKQKGTNQYEKLANKGDYFYVTEYGAKLWVNLTDYLDTGLFLDHRLTRKMVGQMAKGKTFLNLFAYTGSA
TIHAALNGAKSTTTVDMSNTYLNWAEQNLELNNLPLRNNRLFQADCLQWLAECRERFELIFVDPPTFSNSKRMEDSWDVQ
RDHIKLMTQLKRILTTDGTIVFSNNKRGFKMDFEGLAELGLQAENISHKTLPLDFERNPQIHNCWIIRHIEN
>Mature_711_residues
TTQITYFATAARGFEEMLKTELEQICQAECKVTQGGVHFTTTQRGAYQALLHSRLASRILLPLVTTKIFSDLDLYATIVG
INWAYIFDPRDTFFVDFNGTNREIRNTQFGAMRVKDGVVDYFERKGFTRPTVDKDHADIRIHVYLDRENMVVSLDLSGDA
LHMRGYREDTGKAPLRETLAAAIVLRSGWQKGTPLVDPMCGSGTLLIEAAQMQAGIAPQLHRKHWGFNAWKGHQQAVWKE
VLEQAYLQQNEEIQPLFFGFDLDHRVLAKAKQNAKNAGVAHLIQWQQGDIAALKNPCPEQVGTVICNPPYGERLGTTPAL
IALYSVFGQRLKQQFSGWNASIFSGEPELLNCLRLRSHRQFKAKNGPLDCLQKNYQISERTAAEQQADELKFEQNAQVAP
DFANRLAKNIKKIEKWAKQQGINAYRLYDADLPEYNLAVDRYDDHIVVQEYAAPKNIDEQKARQRLLDAVSATLYVTGVE
TNKLVLKVRQKQKGTNQYEKLANKGDYFYVTEYGAKLWVNLTDYLDTGLFLDHRLTRKMVGQMAKGKTFLNLFAYTGSAT
IHAALNGAKSTTTVDMSNTYLNWAEQNLELNNLPLRNNRLFQADCLQWLAECRERFELIFVDPPTFSNSKRMEDSWDVQR
DHIKLMTQLKRILTTDGTIVFSNNKRGFKMDFEGLAELGLQAENISHKTLPLDFERNPQIHNCWIIRHIEN

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=706, Percent_Identity=60.6232294617564, Blast_Score=887, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=258, Percent_Identity=27.5193798449612, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_ACTP2 (A3MYD2)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052773.1
- ProteinModelPortal:   A3MYD2
- SMR:   A3MYD2
- STRING:   A3MYD2
- GeneID:   4850334
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0060
- eggNOG:   COG0116
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- PhylomeDB:   A3MYD2
- ProtClustDB:   PRK11783
- BioCyc:   APLE416269:APL_0060-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 81306; Mature: 81174

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQITYFATAARGFEEMLKTELEQICQAECKVTQGGVHFTTTQRGAYQALLHSRLASRI
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCEEECCCEEEEECCCHHHHHHHHHHHHHHH
LLPLVTTKIFSDLDLYATIVGINWAYIFDPRDTFFVDFNGTNREIRNTQFGAMRVKDGVV
HHHHHHHHHHHCHHHEEEEEECEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEHHHHH
DYFERKGFTRPTVDKDHADIRIHVYLDRENMVVSLDLSGDALHMRGYREDTGKAPLRETL
HHHHHCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCEEEECCCCCCCCCCHHHHHH
AAAIVLRSGWQKGTPLVDPMCGSGTLLIEAAQMQAGIAPQLHRKHWGFNAWKGHQQAVWK
HHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCHHHHHHHCCCCCCCCHHHHHHH
EVLEQAYLQQNEEIQPLFFGFDLDHRVLAKAKQNAKNAGVAHLIQWQQGDIAALKNPCPE
HHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCCCH
QVGTVICNPPYGERLGTTPALIALYSVFGQRLKQQFSGWNASIFSGEPELLNCLRLRSHR
HHCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCC
QFKAKNGPLDCLQKNYQISERTAAEQQADELKFEQNAQVAPDFANRLAKNIKKIEKWAKQ
HHCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QGINAYRLYDADLPEYNLAVDRYDDHIVVQEYAAPKNIDEQKARQRLLDAVSATLYVTGV
CCCCEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHEEEEEEEC
ETNKLVLKVRQKQKGTNQYEKLANKGDYFYVTEYGAKLWVNLTDYLDTGLFLDHRLTRKM
CCCCEEEEEHHHHCCCHHHHHHHCCCCEEEEEECCCEEEEEEHHHHCCCHHHHHHHHHHH
VGQMAKGKTFLNLFAYTGSATIHAALNGAKSTTTVDMSNTYLNWAEQNLELNNLPLRNNR
HHHHHCCCEEEEEEEECCCEEEEEEECCCCCCEEEECCCCCHHHHHCCCEECCCCCCCCC
LFQADCLQWLAECRERFELIFVDPPTFSNSKRMEDSWDVQRDHIKLMTQLKRILTTDGTI
EEHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
VFSNNKRGFKMDFEGLAELGLQAENISHKTLPLDFERNPQIHNCWIIRHIEN
EECCCCCCEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure 
TTQITYFATAARGFEEMLKTELEQICQAECKVTQGGVHFTTTQRGAYQALLHSRLASRI
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHCEEECCCEEEEECCCHHHHHHHHHHHHHHH
LLPLVTTKIFSDLDLYATIVGINWAYIFDPRDTFFVDFNGTNREIRNTQFGAMRVKDGVV
HHHHHHHHHHHCHHHEEEEEECEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEHHHHH
DYFERKGFTRPTVDKDHADIRIHVYLDRENMVVSLDLSGDALHMRGYREDTGKAPLRETL
HHHHHCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCEEEECCCCCCCCCCHHHHHH
AAAIVLRSGWQKGTPLVDPMCGSGTLLIEAAQMQAGIAPQLHRKHWGFNAWKGHQQAVWK
HHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCHHHHHHHCCCCCCCCHHHHHHH
EVLEQAYLQQNEEIQPLFFGFDLDHRVLAKAKQNAKNAGVAHLIQWQQGDIAALKNPCPE
HHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCCCH
QVGTVICNPPYGERLGTTPALIALYSVFGQRLKQQFSGWNASIFSGEPELLNCLRLRSHR
HHCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCC
QFKAKNGPLDCLQKNYQISERTAAEQQADELKFEQNAQVAPDFANRLAKNIKKIEKWAKQ
HHCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QGINAYRLYDADLPEYNLAVDRYDDHIVVQEYAAPKNIDEQKARQRLLDAVSATLYVTGV
CCCCEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHEEEEEEEC
ETNKLVLKVRQKQKGTNQYEKLANKGDYFYVTEYGAKLWVNLTDYLDTGLFLDHRLTRKM
CCCCEEEEEHHHHCCCHHHHHHHCCCCEEEEEECCCEEEEEEHHHHCCCHHHHHHHHHHH
VGQMAKGKTFLNLFAYTGSATIHAALNGAKSTTTVDMSNTYLNWAEQNLELNNLPLRNNR
HHHHHCCCEEEEEEEECCCEEEEEEECCCCCCEEEECCCCCHHHHHCCCEECCCCCCCCC
LFQADCLQWLAECRERFELIFVDPPTFSNSKRMEDSWDVQRDHIKLMTQLKRILTTDGTI
EEHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
VFSNNKRGFKMDFEGLAELGLQAENISHKTLPLDFERNPQIHNCWIIRHIEN
EECCCCCCEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA