Click here to switch to the map view.

The map label for this gene is ddl

Identifier: 126207508

GI number: 126207508

Start: 23415

End: 24326

Strand: Direct

Name: ddl

Synonym: APL_0020

Alternate gene names: 126207508

Gene position: 23415-24326 (Clockwise)

Preceding gene: 126207507

Following gene: 126207509

Centisome position: 1.03

GC content: 44.96

Gene sequence:

>912_bases
ATGAGCATTAAAGACGAAAAAATTGCGGTGTTATTCGGCGGTGTTTCTCAAGAACGCGAAGTATCGCTAAATTCGGGCGC
AGCGGTAACCGAAGCGTTAAAATCGCTTGGCTATAATGTGGAAGGTATTGATACCAAGGATTTCCCGATTGAAAAATTAA
AAGAAAAAGGCATTCAACGCGTATTCAATATTTTACACGGCGGTATCGGCGAAAACGGTGTGTTACAAGGCGCACTAGAG
CAAATGGGGATCCCTTATACCGGTTGCGGCGTAATGGCTTCGGCAGTGACATTAGATAAATTCCGTACCAAATTAATGTG
GCAAGCGGTTGGCCTACCAACAGCAGACATGGTGGTTGTTCGCCGTGGAGAAGCGGTCGATTCCGAACAAATTATTGCAA
AATTAGGTTTACCGGTTTTCGTTAAACCTTCAAGCGAAGGTTCAAGTGTCGGCGTAACCAAAGTAAAAACGGTTGAGCAA
TTATTGCCGGCGGTGGAAGAAGCGTTAAAATTCGATTCAATCGTTTTAGTAGAAGCTTTCTTGGCGGGTAAGGAATATTC
TGTTCCGGTGCTAGACGGTCAAGTATTACCGGCGGTACAAATCATTCCCGAAGGGGAGTTTTATGATTACCACGCTAAAT
ATATCTCGGATAATACGCAATATCTCGTACCGGCGTTAAGTGACGATCGTCAGGCGGAAGTAGCGGAACTGGTCAAAGCA
GCGTATGAGGTGGTCGGATGCCGTGGTTGGAGCCGTATTGATGTGATGGAAGATGCAAATGGTCACTTTAACTTAGTGGA
AGTGAATACTTGTCCGGGTATGACAAGCCATAGTATTTTCCCTAAATCGGCGGCAACGGTCGGCATTCCGTTTGAAAAAT
TAGTCGAACGTGTATTGGAGTTAAGTGCTTAA

Upstream 100 bases:

>100_bases
TAAGATTTGCAAAAAATTTTGTAAAATTGACCGCTTGTGAATCTCCAATCGGTCAGTAGGGATACGAGACCTCGTGTCCA
TAATAAGAAAGGATAAAAAG

Downstream 100 bases:

>100_bases
TGGTTTCTGTTTTACGGAAAAAAACGGCTCAGTCCGTCCGTTTCAAACCGCTAAAAGTGAGATCTTCGCTAAATCCATTC
AATCGGATAGTATTAATTAA

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSIKDEKIAVLFGGVSQEREVSLNSGAAVTEALKSLGYNVEGIDTKDFPIEKLKEKGIQRVFNILHGGIGENGVLQGALE
QMGIPYTGCGVMASAVTLDKFRTKLMWQAVGLPTADMVVVRRGEAVDSEQIIAKLGLPVFVKPSSEGSSVGVTKVKTVEQ
LLPAVEEALKFDSIVLVEAFLAGKEYSVPVLDGQVLPAVQIIPEGEFYDYHAKYISDNTQYLVPALSDDRQAEVAELVKA
AYEVVGCRGWSRIDVMEDANGHFNLVEVNTCPGMTSHSIFPKSAATVGIPFEKLVERVLELSA

Sequences:

>Translated_303_residues
MSIKDEKIAVLFGGVSQEREVSLNSGAAVTEALKSLGYNVEGIDTKDFPIEKLKEKGIQRVFNILHGGIGENGVLQGALE
QMGIPYTGCGVMASAVTLDKFRTKLMWQAVGLPTADMVVVRRGEAVDSEQIIAKLGLPVFVKPSSEGSSVGVTKVKTVEQ
LLPAVEEALKFDSIVLVEAFLAGKEYSVPVLDGQVLPAVQIIPEGEFYDYHAKYISDNTQYLVPALSDDRQAEVAELVKA
AYEVVGCRGWSRIDVMEDANGHFNLVEVNTCPGMTSHSIFPKSAATVGIPFEKLVERVLELSA
>Mature_302_residues
SIKDEKIAVLFGGVSQEREVSLNSGAAVTEALKSLGYNVEGIDTKDFPIEKLKEKGIQRVFNILHGGIGENGVLQGALEQ
MGIPYTGCGVMASAVTLDKFRTKLMWQAVGLPTADMVVVRRGEAVDSEQIIAKLGLPVFVKPSSEGSSVGVTKVKTVEQL
LPAVEEALKFDSIVLVEAFLAGKEYSVPVLDGQVLPAVQIIPEGEFYDYHAKYISDNTQYLVPALSDDRQAEVAELVKAA
YEVVGCRGWSRIDVMEDANGHFNLVEVNTCPGMTSHSIFPKSAATVGIPFEKLVERVLELSA

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=303, Percent_Identity=51.1551155115512, Blast_Score=305, Evalue=2e-84,
Organism=Escherichia coli, GI1786579, Length=346, Percent_Identity=31.2138728323699, Blast_Score=158, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_ACTP2 (A3MY92)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052733.1
- ProteinModelPortal:   A3MY92
- SMR:   A3MY92
- STRING:   A3MY92
- GeneID:   4849242
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0020
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   RAFIILH
- PhylomeDB:   A3MY92
- ProtClustDB:   PRK01372
- BioCyc:   APLE416269:APL_0020-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 32704; Mature: 32573

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKDEKIAVLFGGVSQEREVSLNSGAAVTEALKSLGYNVEGIDTKDFPIEKLKEKGIQR
CCCCCCEEEEEECCCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VFNILHGGIGENGVLQGALEQMGIPYTGCGVMASAVTLDKFRTKLMWQAVGLPTADMVVV
HHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
RRGEAVDSEQIIAKLGLPVFVKPSSEGSSVGVTKVKTVEQLLPAVEEALKFDSIVLVEAF
ECCCCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
LAGKEYSVPVLDGQVLPAVQIIPEGEFYDYHAKYISDNTQYLVPALSDDRQAEVAELVKA
HCCCCCCCEEECCCCCCEEEEEECCCCEEHHHHEECCCCEEEEEECCCCCHHHHHHHHHH
AYEVVGCRGWSRIDVMEDANGHFNLVEVNTCPGMTSHSIFPKSAATVGIPFEKLVERVLE
HHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEECCCHHHHHHHHHH
LSA
HCC
>Mature Secondary Structure 
SIKDEKIAVLFGGVSQEREVSLNSGAAVTEALKSLGYNVEGIDTKDFPIEKLKEKGIQR
CCCCCEEEEEECCCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VFNILHGGIGENGVLQGALEQMGIPYTGCGVMASAVTLDKFRTKLMWQAVGLPTADMVVV
HHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
RRGEAVDSEQIIAKLGLPVFVKPSSEGSSVGVTKVKTVEQLLPAVEEALKFDSIVLVEAF
ECCCCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
LAGKEYSVPVLDGQVLPAVQIIPEGEFYDYHAKYISDNTQYLVPALSDDRQAEVAELVKA
HCCCCCCCEEECCCCCCEEEEEECCCCEEHHHHEECCCCEEEEEECCCCCHHHHHHHHHH
AYEVVGCRGWSRIDVMEDANGHFNLVEVNTCPGMTSHSIFPKSAATVGIPFEKLVERVLE
HHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEECCCHHHHHHHHHH
LSA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA