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The map label for this gene is mraW

Identifier: 126207498

GI number: 126207498

Start: 10398

End: 11339

Strand: Direct

Name: mraW

Synonym: APL_0010

Alternate gene names: 126207498

Gene position: 10398-11339 (Clockwise)

Preceding gene: 126207497

Following gene: 126207499

Centisome position: 0.46

GC content: 44.27

Gene sequence:

>942_bases
ATGAACGACACAGTCCATAAGCATATTACCGTATTGCTACATGAAGCCGTTGACGGTTTGGCGATTAAGCCGAACGGTAT
TTACATTGACGGTACTTTCGGACGAGGCGGTCATTCTCGACTCATTCTCTCAAAACTTTCCGAACAAGGTCGCTTAATTG
CGACGGATCGTGACCCACGTGCAATTGCCGAAGCGAATACGATTAATGATGCTCGTTTTCAAATTGTACATACCGCTTTT
TCTGCAATTCCCGAAGTATGCGAACAATTAGGCTTAACCGGCAAAATTGACGGTATTTTGCTTGATCTCGGCGTTTCTTC
GCCGCAGCTTGATGATGCGGAACGCGGCTTCAGTTTTATGCGTGACGGGCCGCTTGATATGCGTATGGATACCACTAAAG
GTCTATCTGCCGCCGAATGGTTGGCACAAGTGTCGGTGGACGATTTAGCGTGGGTATTAAAAACCTTCGGTGAAGAACGT
TTTGCCAAACGTATTGCACAAGCGGTCGTTTCTTATAATAAGTCTGCAACGGATAAAATCTCTCGTACGCTTCAATTAGC
GCAGATTATTGCCGATGCAGTACCTTTCAAAGATAAGCACAAACATCCGGCAACTCGCTCGTTTCAAGCAATTCGTATCT
TTATTAACAGCGAGTTGGACGAGCTGGAGAAAGCGTTACAGTCGGCATTAAGCGTATTGGCGCCGGAAGGACGTTTATCG
ATTATCAGTTTCCATTCGTTAGAAGATCGAATGGTTAAACAATTTATGAAGAAAAACAGTAAAGGTATGGGCGTGCCGAA
AGGTTTACCTATTTTGGAGTCGGAATTAAATAAAAATATTCCGTTAAAAATCATTGGTAAAGCGATTATGCCGAGCGAAG
CGGAAATCGAAGCAAACCCTCGCTCACGTAGTGCCGTATTACGTATTGCGGAAAAAAGATAG

Upstream 100 bases:

>100_bases
ATTGCGGAAGATCTTGCCTTGGGCGGTTCGGCGGAAATGCTGAATTGCGAAGCCTTAAAAAACCTTTCTTTATAAGATAA
ATAAGTGAGTTGGAAAAATA

Downstream 100 bases:

>100_bases
AGGATTTAGCGAATGGCAAGTAATGAACGTTATCCGCTCCATCAAATTATTTTAGATGATTTAACCGCGCATAATAAGGT
GGCTCTAATCCTGATTATTG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNDTVHKHITVLLHEAVDGLAIKPNGIYIDGTFGRGGHSRLILSKLSEQGRLIATDRDPRAIAEANTINDARFQIVHTAF
SAIPEVCEQLGLTGKIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDTTKGLSAAEWLAQVSVDDLAWVLKTFGEER
FAKRIAQAVVSYNKSATDKISRTLQLAQIIADAVPFKDKHKHPATRSFQAIRIFINSELDELEKALQSALSVLAPEGRLS
IISFHSLEDRMVKQFMKKNSKGMGVPKGLPILESELNKNIPLKIIGKAIMPSEAEIEANPRSRSAVLRIAEKR

Sequences:

>Translated_313_residues
MNDTVHKHITVLLHEAVDGLAIKPNGIYIDGTFGRGGHSRLILSKLSEQGRLIATDRDPRAIAEANTINDARFQIVHTAF
SAIPEVCEQLGLTGKIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDTTKGLSAAEWLAQVSVDDLAWVLKTFGEER
FAKRIAQAVVSYNKSATDKISRTLQLAQIIADAVPFKDKHKHPATRSFQAIRIFINSELDELEKALQSALSVLAPEGRLS
IISFHSLEDRMVKQFMKKNSKGMGVPKGLPILESELNKNIPLKIIGKAIMPSEAEIEANPRSRSAVLRIAEKR
>Mature_313_residues
MNDTVHKHITVLLHEAVDGLAIKPNGIYIDGTFGRGGHSRLILSKLSEQGRLIATDRDPRAIAEANTINDARFQIVHTAF
SAIPEVCEQLGLTGKIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDTTKGLSAAEWLAQVSVDDLAWVLKTFGEER
FAKRIAQAVVSYNKSATDKISRTLQLAQIIADAVPFKDKHKHPATRSFQAIRIFINSELDELEKALQSALSVLAPEGRLS
IISFHSLEDRMVKQFMKKNSKGMGVPKGLPILESELNKNIPLKIIGKAIMPSEAEIEANPRSRSAVLRIAEKR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=338, Percent_Identity=38.4615384615385, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI165377202, Length=192, Percent_Identity=41.1458333333333, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1786270, Length=309, Percent_Identity=66.3430420711974, Blast_Score=418, Evalue=1e-118,
Organism=Drosophila melanogaster, GI62472493, Length=336, Percent_Identity=41.0714285714286, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI28571637, Length=336, Percent_Identity=41.0714285714286, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_ACTP2 (A3MY82)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052723.1
- ProteinModelPortal:   A3MY82
- SMR:   A3MY82
- STRING:   A3MY82
- GeneID:   4849206
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0010
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   A3MY82
- ProtClustDB:   PRK00050
- BioCyc:   APLE416269:APL_0010-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34498; Mature: 34498

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: BINDING 56-56 BINDING 80-80 BINDING 102-102 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTVHKHITVLLHEAVDGLAIKPNGIYIDGTFGRGGHSRLILSKLSEQGRLIATDRDPR
CCCHHHHHHHHHHHHHHCCEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEECCCCH
AIAEANTINDARFQIVHTAFSAIPEVCEQLGLTGKIDGILLDLGVSSPQLDDAERGFSFM
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHCHHHH
RDGPLDMRMDTTKGLSAAEWLAQVSVDDLAWVLKTFGEERFAKRIAQAVVSYNKSATDKI
CCCCCCEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHH
SRTLQLAQIIADAVPFKDKHKHPATRSFQAIRIFINSELDELEKALQSALSVLAPEGRLS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEE
IISFHSLEDRMVKQFMKKNSKGMGVPKGLPILESELNKNIPLKIIGKAIMPSEAEIEANP
EEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCEEECHHHCCCCCCCCCCC
RSRSAVLRIAEKR
CHHHHHHHHHCCC
>Mature Secondary Structure
MNDTVHKHITVLLHEAVDGLAIKPNGIYIDGTFGRGGHSRLILSKLSEQGRLIATDRDPR
CCCHHHHHHHHHHHHHHCCEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEECCCCH
AIAEANTINDARFQIVHTAFSAIPEVCEQLGLTGKIDGILLDLGVSSPQLDDAERGFSFM
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHCHHHH
RDGPLDMRMDTTKGLSAAEWLAQVSVDDLAWVLKTFGEERFAKRIAQAVVSYNKSATDKI
CCCCCCEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHH
SRTLQLAQIIADAVPFKDKHKHPATRSFQAIRIFINSELDELEKALQSALSVLAPEGRLS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEE
IISFHSLEDRMVKQFMKKNSKGMGVPKGLPILESELNKNIPLKIIGKAIMPSEAEIEANP
EEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCEEECHHHCCCCCCCCCCC
RSRSAVLRIAEKR
CHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA