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The map label for this gene is recF

Identifier: 126207491

GI number: 126207491

Start: 3001

End: 4083

Strand: Direct

Name: recF

Synonym: APL_0003

Alternate gene names: 126207491

Gene position: 3001-4083 (Clockwise)

Preceding gene: 126207490

Following gene: 126207493

Centisome position: 0.13

GC content: 43.86

Gene sequence:

>1083_bases
ATGCCCCTATCCAGATTAATCATCAATAATTTCCGAAATCTCCAATCTCTCGATCTTGAACTCAGTCCTAATTTTAATTT
TATCGTAGGGCATAACGGCAGCGGTAAAACCAGTTTGTTGGAAGCGATTTTTTACTTAGGGCACGGTCGGTCATTTAAAA
GTCATATCAGTAATCGAATTATTCACTACCAAGCAGAAGATTTTGTGTTGCACGCTCGTATTGATGAAGGGCAACATCAA
TGGTCGGTCGGGATCCAGAAAAAACGTTCGGGCGATACGCTGTTAAAAATTAATGGTGAAGACGGCAATAAAATTTCTGA
TTTGGCGCACCTACTGCCGATGCAAGTCATTACGCCGGAAGGGCTAACCTTATTAAATGGCGGACCGACTTTCCGTCGAG
CATTTTTAGACTGGGGGTTATTCCATCAATATACCGAGTTTTATAGCTGTTGGGCAAATTTAAAACGTTTACTCAAGCAA
CGCAATGCAGCATTGCATCAAGTTCGCAGTTATGCCGAATTAAAGCCGTGGGATACTGAATTGGCGAAATTGGCGGAGAT
TGTCAGCCAAATGCGTGCGAATTATGCGGAAGCGTTACGACCGGAAATTGAGAAAACCTGTCAGTTTTTCTTACCTGAAT
TGGAAATAGGGGTGAGTTTCCATCAAGGCTGGGAGAAAGGGACGGATTATGCCGAGATTCTTGCTCAAGGTTTTGAGCGA
GATAAAGCCATGGGCTATACCATGATTGGTCCGCAAAAGGCGGATTTTCGCTTTCGGGCGAACGGTTTACCGGTGGAAGA
TGTGCTTTCTCGTGGGCAGCTTAAATTACTGATGTGTGTGTTGCGTTTGGCACAAGGCGAATATTTAGTGGCGCAAAAAG
AACGTCAGTGTTTATTCCTGATCGATGATTTCGCTTCCGAGTTGGACCCGATTAAACGGGAACTGTTGGCACATCGTTTA
CGAGAAAGCGGTTCGCAAGTATTTGTGACGGCGATTACCAAAGACCAGCTAAACCAAATGCAATGGCAAGAAAGCGAACA
AGATAGTCTGTTCCAAGTTCAACAAGGTATGCTGACTAAATAA

Upstream 100 bases:

>100_bases
AAAAATGACCGCTTGTAAAAGCGATTTTATCAGCGGTCATTTGTGAATTATTGCACCAATCAGAATTGCTTTAGAACACT
CGATCTTAGAGAGTCCATAT

Downstream 100 bases:

>100_bases
AAAAGGGATGTGTTACACATCCCTTTTTTCATCGGTTTAAATTTATTTAATTATGCTATTTGATGACGCCGCAAGCCATA
CGCGGACCGCCACCGCCTAA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MPLSRLIINNFRNLQSLDLELSPNFNFIVGHNGSGKTSLLEAIFYLGHGRSFKSHISNRIIHYQAEDFVLHARIDEGQHQ
WSVGIQKKRSGDTLLKINGEDGNKISDLAHLLPMQVITPEGLTLLNGGPTFRRAFLDWGLFHQYTEFYSCWANLKRLLKQ
RNAALHQVRSYAELKPWDTELAKLAEIVSQMRANYAEALRPEIEKTCQFFLPELEIGVSFHQGWEKGTDYAEILAQGFER
DKAMGYTMIGPQKADFRFRANGLPVEDVLSRGQLKLLMCVLRLAQGEYLVAQKERQCLFLIDDFASELDPIKRELLAHRL
RESGSQVFVTAITKDQLNQMQWQESEQDSLFQVQQGMLTK

Sequences:

>Translated_360_residues
MPLSRLIINNFRNLQSLDLELSPNFNFIVGHNGSGKTSLLEAIFYLGHGRSFKSHISNRIIHYQAEDFVLHARIDEGQHQ
WSVGIQKKRSGDTLLKINGEDGNKISDLAHLLPMQVITPEGLTLLNGGPTFRRAFLDWGLFHQYTEFYSCWANLKRLLKQ
RNAALHQVRSYAELKPWDTELAKLAEIVSQMRANYAEALRPEIEKTCQFFLPELEIGVSFHQGWEKGTDYAEILAQGFER
DKAMGYTMIGPQKADFRFRANGLPVEDVLSRGQLKLLMCVLRLAQGEYLVAQKERQCLFLIDDFASELDPIKRELLAHRL
RESGSQVFVTAITKDQLNQMQWQESEQDSLFQVQQGMLTK
>Mature_359_residues
PLSRLIINNFRNLQSLDLELSPNFNFIVGHNGSGKTSLLEAIFYLGHGRSFKSHISNRIIHYQAEDFVLHARIDEGQHQW
SVGIQKKRSGDTLLKINGEDGNKISDLAHLLPMQVITPEGLTLLNGGPTFRRAFLDWGLFHQYTEFYSCWANLKRLLKQR
NAALHQVRSYAELKPWDTELAKLAEIVSQMRANYAEALRPEIEKTCQFFLPELEIGVSFHQGWEKGTDYAEILAQGFERD
KAMGYTMIGPQKADFRFRANGLPVEDVLSRGQLKLLMCVLRLAQGEYLVAQKERQCLFLIDDFASELDPIKRELLAHRLR
ESGSQVFVTAITKDQLNQMQWQESEQDSLFQVQQGMLTK

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=359, Percent_Identity=55.7103064066852, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_ACTP2 (A3MY75)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052716.1
- STRING:   A3MY75
- GeneID:   4848972
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0003
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- PhylomeDB:   A3MY75
- ProtClustDB:   PRK00064
- BioCyc:   APLE416269:APL_0003-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41301; Mature: 41170

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSRLIINNFRNLQSLDLELSPNFNFIVGHNGSGKTSLLEAIFYLGHGRSFKSHISNRI
CCHHHHHHHHCCCHHHCCEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHCCE
IHYQAEDFVLHARIDEGQHQWSVGIQKKRSGDTLLKINGEDGNKISDLAHLLPMQVITPE
EEEECCCEEEEEEECCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHEECCC
GLTLLNGGPTFRRAFLDWGLFHQYTEFYSCWANLKRLLKQRNAALHQVRSYAELKPWDTE
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LAKLAEIVSQMRANYAEALRPEIEKTCQFFLPELEIGVSFHQGWEKGTDYAEILAQGFER
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHCCHHHHCCCCCCCHHHHHHHHHHH
DKAMGYTMIGPQKADFRFRANGLPVEDVLSRGQLKLLMCVLRLAQGEYLVAQKERQCLFL
HHHCCEEEECCCCCCCEEECCCCCHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCEEEE
IDDFASELDPIKRELLAHRLRESGSQVFVTAITKDQLNQMQWQESEQDSLFQVQQGMLTK
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
PLSRLIINNFRNLQSLDLELSPNFNFIVGHNGSGKTSLLEAIFYLGHGRSFKSHISNRI
CHHHHHHHHCCCHHHCCEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHCCE
IHYQAEDFVLHARIDEGQHQWSVGIQKKRSGDTLLKINGEDGNKISDLAHLLPMQVITPE
EEEECCCEEEEEEECCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHEECCC
GLTLLNGGPTFRRAFLDWGLFHQYTEFYSCWANLKRLLKQRNAALHQVRSYAELKPWDTE
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LAKLAEIVSQMRANYAEALRPEIEKTCQFFLPELEIGVSFHQGWEKGTDYAEILAQGFER
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHCCHHHHCCCCCCCHHHHHHHHHHH
DKAMGYTMIGPQKADFRFRANGLPVEDVLSRGQLKLLMCVLRLAQGEYLVAQKERQCLFL
HHHCCEEEECCCCCCCEEECCCCCHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCEEEE
IDDFASELDPIKRELLAHRLRESGSQVFVTAITKDQLNQMQWQESEQDSLFQVQQGMLTK
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA