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The map label for this gene is dnaN [H]

Identifier: 126207490

GI number: 126207490

Start: 1564

End: 2667

Strand: Direct

Name: dnaN [H]

Synonym: APL_0002

Alternate gene names: 126207490

Gene position: 1564-2667 (Clockwise)

Preceding gene: 126207489

Following gene: 126207491

Centisome position: 0.07

GC content: 44.47

Gene sequence:

>1104_bases
ATGCAGTTTACGATAACCAGAGAACAGTTACTTAAGCCTTTACAACAGGTCTGTGGCGTATTAAGCAGCCGCCCGACGTT
GCCTGTGATTAATAATATTTTGCTTGAAATCGAAGGCGACCGCTTATCGCTAACCGGAACGGACTTAGAAGTGGAATTAA
CTACGGTGGCAACCCTTGAACAAGCGGTTGATTTTGCCGGAAAATTTACCATTCCTGCCAAGAAATTTCTCGATATTTGC
CGTAGCTTGCCGGAAGATAGCAGCATTTCGGTACAATTCGAAGAAGATCGTGCGTTAGTTAGAGCGGCTCGCAGTAAATT
TAATTTAGCCACATTGCCGGCAAGCGATTATCCGAATTTAATGGATTGGAAGCCGGAAGTGGATTTTTCGATTGAACAAT
CCACCCTTGCTCGTTTGATTGATGCGACGCAATTCTCAATGGCGAACCAAGATGCGCGTTATTTTCTAAACGGGATGAAG
TTCGAAACCGAAGGTAATTTATTGCGTACCGTCGCAACGGACGGACACCGTCTAGCGGTTTGTACCATGGCGTTAAATCA
AGAGTTATTAACCCATTCGGTCATTGTGCCTCGTAAAGCGGTATTAGAATTATCACGCCTTGTCGCCGTGAGTGATGAAA
CGGTACGTTTAGAAATCGGCACCAGTAATTTACGTGTGTCAATGAACGGCGTGGTGTTTACCTCTAAACTGATTGACGGG
CGTTTCCCAGACTATCGCCGTGTATTACCACGCAATGCGGATCGTATTTTAGAAGCGGAAACCGAAGTGTTAAAACGTGC
ATTAATGCGTGCGGCAATTTTATCGAATGAAAAATTCCGTGGCGTGCGTTTAGCGTTAAGCGAAAACTTACTCAAAATTA
CCGCCAATAACCCGGAACAAGAAGAAGCGGAAGAGATCATTGACGTGGCATATCAAAGCCCGGAAATGGAAGTCGGCTTT
AATGTGAGTTATTTGTTAGACGTGCTGAATACCTTAAAATGCGAGCGTGTGCGTTTCAATTTGGTGGATGCAAGTTCAAG
CTGCTTAATTGAAGATTGTGATAACAGCACCGCCGAATATGTGATTATGCCGATGCGTCTGTAA

Upstream 100 bases:

>100_bases
TGCAAAACGATTAATGAATTACGTGATACCGATAGCGGTATTCAAGAAGATTATACCAACTTAACCAGAAAATTATCGTC
ATAGGCGATAAGGGATTATT

Downstream 100 bases:

>100_bases
TTTGAATGTTACTTTCTTTGCTTCGCCAAAGAAAGTAACCCCTATACATTATTAACAGAAAGGCGACCCCAGATTTTCCT
TTTTCTTCCGCTTATTGCTA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MQFTITREQLLKPLQQVCGVLSSRPTLPVINNILLEIEGDRLSLTGTDLEVELTTVATLEQAVDFAGKFTIPAKKFLDIC
RSLPEDSSISVQFEEDRALVRAARSKFNLATLPASDYPNLMDWKPEVDFSIEQSTLARLIDATQFSMANQDARYFLNGMK
FETEGNLLRTVATDGHRLAVCTMALNQELLTHSVIVPRKAVLELSRLVAVSDETVRLEIGTSNLRVSMNGVVFTSKLIDG
RFPDYRRVLPRNADRILEAETEVLKRALMRAAILSNEKFRGVRLALSENLLKITANNPEQEEAEEIIDVAYQSPEMEVGF
NVSYLLDVLNTLKCERVRFNLVDASSSCLIEDCDNSTAEYVIMPMRL

Sequences:

>Translated_367_residues
MQFTITREQLLKPLQQVCGVLSSRPTLPVINNILLEIEGDRLSLTGTDLEVELTTVATLEQAVDFAGKFTIPAKKFLDIC
RSLPEDSSISVQFEEDRALVRAARSKFNLATLPASDYPNLMDWKPEVDFSIEQSTLARLIDATQFSMANQDARYFLNGMK
FETEGNLLRTVATDGHRLAVCTMALNQELLTHSVIVPRKAVLELSRLVAVSDETVRLEIGTSNLRVSMNGVVFTSKLIDG
RFPDYRRVLPRNADRILEAETEVLKRALMRAAILSNEKFRGVRLALSENLLKITANNPEQEEAEEIIDVAYQSPEMEVGF
NVSYLLDVLNTLKCERVRFNLVDASSSCLIEDCDNSTAEYVIMPMRL
>Mature_367_residues
MQFTITREQLLKPLQQVCGVLSSRPTLPVINNILLEIEGDRLSLTGTDLEVELTTVATLEQAVDFAGKFTIPAKKFLDIC
RSLPEDSSISVQFEEDRALVRAARSKFNLATLPASDYPNLMDWKPEVDFSIEQSTLARLIDATQFSMANQDARYFLNGMK
FETEGNLLRTVATDGHRLAVCTMALNQELLTHSVIVPRKAVLELSRLVAVSDETVRLEIGTSNLRVSMNGVVFTSKLIDG
RFPDYRRVLPRNADRILEAETEVLKRALMRAAILSNEKFRGVRLALSENLLKITANNPEQEEAEEIIDVAYQSPEMEVGF
NVSYLLDVLNTLKCERVRFNLVDASSSCLIEDCDNSTAEYVIMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=367, Percent_Identity=62.6702997275204, Blast_Score=486, Evalue=1e-139,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41199; Mature: 41199

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFTITREQLLKPLQQVCGVLSSRPTLPVINNILLEIEGDRLSLTGTDLEVELTTVATLE
CCEEECHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEEECCEEEEECCCEEEEEHHHHHHH
QAVDFAGKFTIPAKKFLDICRSLPEDSSISVQFEEDRALVRAARSKFNLATLPASDYPNL
HHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCEEECCCCCCCCC
MDWKPEVDFSIEQSTLARLIDATQFSMANQDARYFLNGMKFETEGNLLRTVATDGHRLAV
CCCCCCCCCEECHHHHHHHHHHHHHHHCCCCHHHHHCCEEEECCCCEEEEEECCCCEEEE
CTMALNQELLTHSVIVPRKAVLELSRLVAVSDETVRLEIGTSNLRVSMNGVVFTSKLIDG
EEEHHHHHHHHHHHHCCHHHHHHHHHHHEECCCEEEEEECCCEEEEEECCEEEEEHHHCC
RFPDYRRVLPRNADRILEAETEVLKRALMRAAILSNEKFRGVRLALSENLLKITANNPEQ
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCH
EEAEEIIDVAYQSPEMEVGFNVSYLLDVLNTLKCERVRFNLVDASSSCLIEDCDNSTAEY
HHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHEEEEEECCCCCEEEECCCCCCEEE
VIMPMRL
EEEEECC
>Mature Secondary Structure
MQFTITREQLLKPLQQVCGVLSSRPTLPVINNILLEIEGDRLSLTGTDLEVELTTVATLE
CCEEECHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEEECCEEEEECCCEEEEEHHHHHHH
QAVDFAGKFTIPAKKFLDICRSLPEDSSISVQFEEDRALVRAARSKFNLATLPASDYPNL
HHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCEEECCCCCCCCC
MDWKPEVDFSIEQSTLARLIDATQFSMANQDARYFLNGMKFETEGNLLRTVATDGHRLAV
CCCCCCCCCEECHHHHHHHHHHHHHHHCCCCHHHHHCCEEEECCCCEEEEEECCCCEEEE
CTMALNQELLTHSVIVPRKAVLELSRLVAVSDETVRLEIGTSNLRVSMNGVVFTSKLIDG
EEEHHHHHHHHHHHHCCHHHHHHHHHHHEECCCEEEEEECCCEEEEEECCEEEEEHHHCC
RFPDYRRVLPRNADRILEAETEVLKRALMRAAILSNEKFRGVRLALSENLLKITANNPEQ
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCH
EEAEEIIDVAYQSPEMEVGFNVSYLLDVLNTLKCERVRFNLVDASSSCLIEDCDNSTAEY
HHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHEEEEEECCCCCEEEECCCCCCEEE
VIMPMRL
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]