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The map label for this gene is napB [H]

Identifier: 126175709

GI number: 126175709

Start: 4120568

End: 4120999

Strand: Direct

Name: napB [H]

Synonym: Sbal_3512

Alternate gene names: 126175709

Gene position: 4120568-4120999 (Clockwise)

Preceding gene: 126175708

Following gene: 126175711

Centisome position: 80.36

GC content: 47.69

Gene sequence:

>432_bases
ATGAGAAAAATACTCACCTTAACAGCGTTACTCGTCGCCATTACCGGCTGTTCAGGCCAGCAAACCGACACCAACGTAGC
ACCAGTTAATATTCAATCCCTCGCAAGAAACATGGCGATCACCGACATTCGCCCAGCCGATGCTATGGCGATTTATCCTA
ATCGCGGCTCCGCCATTGAGCGCAGCTTTAGCGAGCAACCACCGCTTATCCCACATAAGGACGATTACAAGATCACCTTA
GATAAAAATGGTTGTTTAAGCTGCCATAGTTGGGACAAGGCAAAACGCATGAAAGCCACGCCTGTCGCTAAGTCCCATGT
GATTGACGATAAAGGCACAGTTAACGGGCATAACTATTTCTGTACTCAATGCCACGTCGCTCAAGCCGAAAACAAACAAC
CACTCGTGGAGAATCAGTTCTCAGACAAGTAA

Upstream 100 bases:

>100_bases
AGTTAGTCAACAAACTGATATTGGATGCGACCGATCCCCTCTCAAAAGAAACTGACTTTAAAAAGTGCCCAGTCAAAGTC
TTCAAGGCGGAGAATAACCA

Downstream 100 bases:

>100_bases
ATCACCTTCCCCTGCAAAGCAAAGCCACCCGAATTTATGAGGGTGGCTTTTTGCTATTTAAGCATCCCATCGTAATGAGT
TGGCTTACTGTCGGTGATGA

Product: nitrate reductase cytochrome c-type subunit (NapB)

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MRKILTLTALLVAITGCSGQQTDTNVAPVNIQSLARNMAITDIRPADAMAIYPNRGSAIERSFSEQPPLIPHKDDYKITL
DKNGCLSCHSWDKAKRMKATPVAKSHVIDDKGTVNGHNYFCTQCHVAQAENKQPLVENQFSDK

Sequences:

>Translated_143_residues
MRKILTLTALLVAITGCSGQQTDTNVAPVNIQSLARNMAITDIRPADAMAIYPNRGSAIERSFSEQPPLIPHKDDYKITL
DKNGCLSCHSWDKAKRMKATPVAKSHVIDDKGTVNGHNYFCTQCHVAQAENKQPLVENQFSDK
>Mature_143_residues
MRKILTLTALLVAITGCSGQQTDTNVAPVNIQSLARNMAITDIRPADAMAIYPNRGSAIERSFSEQPPLIPHKDDYKITL
DKNGCLSCHSWDKAKRMKATPVAKSHVIDDKGTVNGHNYFCTQCHVAQAENKQPLVENQFSDK

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=94, Percent_Identity=34.0425531914894, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 15780; Mature: 15780

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKILTLTALLVAITGCSGQQTDTNVAPVNIQSLARNMAITDIRPADAMAIYPNRGSAIE
CCHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCEEEEECCCCCHHH
RSFSEQPPLIPHKDDYKITLDKNGCLSCHSWDKAKRMKATPVAKSHVIDDKGTVNGHNYF
HHHCCCCCCCCCCCCCEEEECCCCCEECCCCCHHHHCCCCCCCCCEEECCCCCCCCCCEE
CTQCHVAQAENKQPLVENQFSDK
EEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKILTLTALLVAITGCSGQQTDTNVAPVNIQSLARNMAITDIRPADAMAIYPNRGSAIE
CCHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCEEEEECCCCCHHH
RSFSEQPPLIPHKDDYKITLDKNGCLSCHSWDKAKRMKATPVAKSHVIDDKGTVNGHNYF
HHHCCCCCCCCCCCCCEEEECCCCCEECCCCCHHHHCCCCCCCCCEEECCCCCCCCCCEE
CTQCHVAQAENKQPLVENQFSDK
EEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488 [H]