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The map label for this gene is napA [H]

Identifier: 126175708

GI number: 126175708

Start: 4118076

End: 4120571

Strand: Direct

Name: napA [H]

Synonym: Sbal_3511

Alternate gene names: 126175708

Gene position: 4118076-4120571 (Clockwise)

Preceding gene: 126175707

Following gene: 126175709

Centisome position: 80.32

GC content: 50.24

Gene sequence:

>2496_bases
ATGAGCATAAGCCGCCGCGAGTTTCTCAAGGCCAACGCAGCGGTTGCCGCCGCAACAGCCGTTGGTGTCACGCTTCCCAT
TAAAATGGTTGAAGCCGCCGAACAGCCAGACAATATCAAATGGGATAAAGCCCCTTGCCGCTTTTGCGGTGTGGGTTGTA
GCGTATTAGTCGGCACCAAAGCGGGTAAAGTCGTTGCTACTAAGGGCGATCCTGAAAGTCCAGTCAACCGTGGCCTTAAC
TGTATCAAGGGCTATTTTTTATCGAAAATCATGTACGGCAAGGACAGATTAACCACGCCATTACTGCGGATGAAAGACGG
CAAATACCATAAAGAAGGCGAGTTCACCCCAATCAGCTGGGATATGGCCTTTGATACTATGGCGGCTAAATGGAAACACA
GTCTCGCCACCAAGGGCCCGACATCAGTGGGTATGTTTGGCTCTGGCCAATGGACCATTTGGGAAGGCTACGCCGCCTCT
AAACTCCATAAAGCAGGTTTTCTAACGAACAATATCGACCCTAATGCGCGCCACTGCATGGCCTCTGCCGTTGGCGGCTT
TATGCGCACCTTCGGTATTGATGAGCCCATGGGGTGTTATGACGATTTAGAAGCCGCCGATCAGTTTGTGCTTTGGGGCG
CGAACATGGCCGAGATGCACCCAATTCTATGGGCACGTTTATCCGATAGACGTTTAAGCAGCCCAACGTGCCGAGTGCAT
GTGCTATCGACCTTTGAAAATCGCAGCTTCGATTTAGCCGATAATCCTATGGTGTTCCGCCCGCAATCGGACTTAGTGAT
CCTCAACTTTATTGCCAATTACATTATTCAAAATAACGCAGTTAATACTGACTTTGTCACTAAACACACTAAGTTTGCCT
TAGGCGTTGACGATATCGGCTATGGCCTGCGTCCAGATCATCCATTAGAGAAAAAAGCCAAAAATCCAGGCAATGGTAAA
TCGACACCGATTAGCTTTGACGAGTATGCCAAGTTTGTCAGCACATATACGCTGGAATACGCGGCTAAGATGAGTGGTGT
GGAGCCCGAGAAATTAGAAACTTTAGCCAAGGCCTATGCCGACCCTAACGTTAAAGTGATGAGTCTGTGGACCATGGGCA
TTAACCAACATGTTCGCGGTGTATGGGCAAACAACATGCTCTACAACATCCACTTACTCACGGGCAAAATTGCCACACCG
GGTAACAGTCCATTCTCGTTAACCGGTCAACCTTCGGCTTGCGGGACTGCACGTGAAGTCGGCACCTTTGCCCATCGTTT
ACCAGCCGATATGGAAGTCACTAACGACAAACACCGCGCCATTACCGAAAAACTATGGCAAGTACCTGAAGGGACTATTC
CCGCCAAACCTGGCTACCATGCCGTACTCCAGAGCCGTATGCTCAAAGATGGCAAGCTCAACTGCTACTGGACCATGTGC
ACCAACAACATGCAGGCGGGTCCCAACATCAACGATGAAATTTATCCGGGTTTCCGCAATCCAGAAAACTTTATCGTGGT
TTCAGATCCCTATCCAACCATCACGGCCATGGCGGCGGATTTAATTTTGCCAACGGCTATGTGGGTCGAAAAAGAAGGCG
CTTACGGCAACGCTGAGCGCCGCACCCACATGTGGCACCAACAGGTTAAAGCCCCCGAAGGCGCGACCTCAGATCTGTGG
CAATTAGTCGAGTTTTCAAAACGTTTTAAAGTATCCGAAGTCTGGCCAGCAGAACTGATAGCCAAACAACCGGAATACGC
GGATAAAACCTTATATGAAGTGCTATTTGCCAATGGCGTGATCGACAAGTTCCCAACTTCCGATTGCAAAGCCGAATTAA
ACGATGAGAGCGAACACTTTGGTTTCTATCTGCAAAAAGGCATTTTTGAAGAATACGCCACATTCGGTCGTGATCACGGC
CATGATCTAGCTGACTTTGACCGTTACCACGAAACCCGCGGCCTACGCTGGCCAGTCGTTAATGGCAAAGAAACCCTGCG
CCGTTTCGTTGAGGGGAGCGACCCTTATGTCAAAGCGGGTGAAGGCTTTAAATTCTATGGGAAGCCAGATGGTAAAGCGG
TGATCTTCGCCCTGCCCTACGAACCCGCCGCCGAAGAGCCGAATGAAGAATACGATCTGTGGATGTCGACTGGCCGCGTG
CTGGAACATTGGCACACAGGCTCGATGACAGCCCGCGTACCTGAGCTTTACCGCGCCTTTCCCGACGCGCAGATTTTTAT
GCATCCAGAAGATGCCAAAGCCCGCGGCGTACAACGCGGCGATGAAGTCATAGTCGCATCACCACGCGGTGAAGTAAAAA
CCCGTGTCGAGACCAAAGGCCGTAACAAACCGCCACGCGGCGTGGTATTTATGCCTTTCTTCGATGCACGCCAGTTAGTC
AACAAACTGATATTGGATGCGACCGATCCCCTCTCAAAAGAAACTGACTTTAAAAAGTGCCCAGTCAAAGTCTTCAAGGC
GGAGAATAACCAATGA

Upstream 100 bases:

>100_bases
TCGACAATATTGAAGCCATCAATGCCCTGTCCGGCGTGTTATCTGCCAGTTTGATTTACCACCAAGTCGCACCTTTAGAA
CAAGAGAGTGAGGAAAGATT

Downstream 100 bases:

>100_bases
GAAAAATACTCACCTTAACAGCGTTACTCGTCGCCATTACCGGCTGTTCAGGCCAGCAAACCGACACCAACGTAGCACCA
GTTAATATTCAATCCCTCGC

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 831; Mature: 830

Protein sequence:

>831_residues
MSISRREFLKANAAVAAATAVGVTLPIKMVEAAEQPDNIKWDKAPCRFCGVGCSVLVGTKAGKVVATKGDPESPVNRGLN
CIKGYFLSKIMYGKDRLTTPLLRMKDGKYHKEGEFTPISWDMAFDTMAAKWKHSLATKGPTSVGMFGSGQWTIWEGYAAS
KLHKAGFLTNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTCRVH
VLSTFENRSFDLADNPMVFRPQSDLVILNFIANYIIQNNAVNTDFVTKHTKFALGVDDIGYGLRPDHPLEKKAKNPGNGK
STPISFDEYAKFVSTYTLEYAAKMSGVEPEKLETLAKAYADPNVKVMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATP
GNSPFSLTGQPSACGTAREVGTFAHRLPADMEVTNDKHRAITEKLWQVPEGTIPAKPGYHAVLQSRMLKDGKLNCYWTMC
TNNMQAGPNINDEIYPGFRNPENFIVVSDPYPTITAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGATSDLW
QLVEFSKRFKVSEVWPAELIAKQPEYADKTLYEVLFANGVIDKFPTSDCKAELNDESEHFGFYLQKGIFEEYATFGRDHG
HDLADFDRYHETRGLRWPVVNGKETLRRFVEGSDPYVKAGEGFKFYGKPDGKAVIFALPYEPAAEEPNEEYDLWMSTGRV
LEHWHTGSMTARVPELYRAFPDAQIFMHPEDAKARGVQRGDEVIVASPRGEVKTRVETKGRNKPPRGVVFMPFFDARQLV
NKLILDATDPLSKETDFKKCPVKVFKAENNQ

Sequences:

>Translated_831_residues
MSISRREFLKANAAVAAATAVGVTLPIKMVEAAEQPDNIKWDKAPCRFCGVGCSVLVGTKAGKVVATKGDPESPVNRGLN
CIKGYFLSKIMYGKDRLTTPLLRMKDGKYHKEGEFTPISWDMAFDTMAAKWKHSLATKGPTSVGMFGSGQWTIWEGYAAS
KLHKAGFLTNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTCRVH
VLSTFENRSFDLADNPMVFRPQSDLVILNFIANYIIQNNAVNTDFVTKHTKFALGVDDIGYGLRPDHPLEKKAKNPGNGK
STPISFDEYAKFVSTYTLEYAAKMSGVEPEKLETLAKAYADPNVKVMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATP
GNSPFSLTGQPSACGTAREVGTFAHRLPADMEVTNDKHRAITEKLWQVPEGTIPAKPGYHAVLQSRMLKDGKLNCYWTMC
TNNMQAGPNINDEIYPGFRNPENFIVVSDPYPTITAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGATSDLW
QLVEFSKRFKVSEVWPAELIAKQPEYADKTLYEVLFANGVIDKFPTSDCKAELNDESEHFGFYLQKGIFEEYATFGRDHG
HDLADFDRYHETRGLRWPVVNGKETLRRFVEGSDPYVKAGEGFKFYGKPDGKAVIFALPYEPAAEEPNEEYDLWMSTGRV
LEHWHTGSMTARVPELYRAFPDAQIFMHPEDAKARGVQRGDEVIVASPRGEVKTRVETKGRNKPPRGVVFMPFFDARQLV
NKLILDATDPLSKETDFKKCPVKVFKAENNQ
>Mature_830_residues
SISRREFLKANAAVAAATAVGVTLPIKMVEAAEQPDNIKWDKAPCRFCGVGCSVLVGTKAGKVVATKGDPESPVNRGLNC
IKGYFLSKIMYGKDRLTTPLLRMKDGKYHKEGEFTPISWDMAFDTMAAKWKHSLATKGPTSVGMFGSGQWTIWEGYAASK
LHKAGFLTNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTCRVHV
LSTFENRSFDLADNPMVFRPQSDLVILNFIANYIIQNNAVNTDFVTKHTKFALGVDDIGYGLRPDHPLEKKAKNPGNGKS
TPISFDEYAKFVSTYTLEYAAKMSGVEPEKLETLAKAYADPNVKVMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATPG
NSPFSLTGQPSACGTAREVGTFAHRLPADMEVTNDKHRAITEKLWQVPEGTIPAKPGYHAVLQSRMLKDGKLNCYWTMCT
NNMQAGPNINDEIYPGFRNPENFIVVSDPYPTITAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGATSDLWQ
LVEFSKRFKVSEVWPAELIAKQPEYADKTLYEVLFANGVIDKFPTSDCKAELNDESEHFGFYLQKGIFEEYATFGRDHGH
DLADFDRYHETRGLRWPVVNGKETLRRFVEGSDPYVKAGEGFKFYGKPDGKAVIFALPYEPAAEEPNEEYDLWMSTGRVL
EHWHTGSMTARVPELYRAFPDAQIFMHPEDAKARGVQRGDEVIVASPRGEVKTRVETKGRNKPPRGVVFMPFFDARQLVN
KLILDATDPLSKETDFKKCPVKVFKAENNQ

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=827, Percent_Identity=73.0350665054414, Blast_Score=1275, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=793, Percent_Identity=23.7074401008827, Blast_Score=192, Evalue=9e-50,
Organism=Escherichia coli, GI3868720, Length=663, Percent_Identity=23.3785822021116, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI3868719, Length=511, Percent_Identity=25.0489236790607, Blast_Score=107, Evalue=4e-24,
Organism=Escherichia coli, GI87081797, Length=833, Percent_Identity=21.7286914765906, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787870, Length=812, Percent_Identity=22.4137931034483, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 93036; Mature: 92905

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISRREFLKANAAVAAATAVGVTLPIKMVEAAEQPDNIKWDKAPCRFCGVGCSVLVGTK
CCCCHHHHHHHHHHHHHHHHHCEEEHHHHHHHCCCCCCCCCCCCCCHHHCCCCEEEEECC
AGKVVATKGDPESPVNRGLNCIKGYFLSKIMYGKDRLTTPLLRMKDGKYHKEGEFTPISW
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHCCCCCCCCCCCCCEEEH
DMAFDTMAAKWKHSLATKGPTSVGMFGSGQWTIWEGYAASKLHKAGFLTNNIDPNARHCM
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEECCCCCCHHHHH
ASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTCRVH
HHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEE
VLSTFENRSFDLADNPMVFRPQSDLVILNFIANYIIQNNAVNTDFVTKHTKFALGVDDIG
EEEECCCCCCCCCCCCEEECCCCCEEHHHHHHHHHHCCCCCCCCCEECCCEEEECHHHCC
YGLRPDHPLEKKAKNPGNGKSTPISFDEYAKFVSTYTLEYAAKMSGVEPEKLETLAKAYA
CCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
DPNVKVMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATPGNSPFSLTGQPSACGTAREV
CCCEEEEEEEEECCCHHHCEEEECCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCHHHH
GTFAHRLPADMEVTNDKHRAITEKLWQVPEGTIPAKPGYHAVLQSRMLKDGKLNCYWTMC
HHHHHHCCCCCEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEE
TNNMQAGPNINDEIYPGFRNPENFIVVSDPYPTITAMAADLILPTAMWVEKEGAYGNAER
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHH
RTHMWHQQVKAPEGATSDLWQLVEFSKRFKVSEVWPAELIAKQPEYADKTLYEVLFANGV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCC
IDKFPTSDCKAELNDESEHFGFYLQKGIFEEYATFGRDHGHDLADFDRYHETRGLRWPVV
CCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCC
NGKETLRRFVEGSDPYVKAGEGFKFYGKPDGKAVIFALPYEPAAEEPNEEYDLWMSTGRV
CCHHHHHHHHCCCCCEEECCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
LEHWHTGSMTARVPELYRAFPDAQIFMHPEDAKARGVQRGDEVIVASPRGEVKTRVETKG
HHHHCCCCCCHHHHHHHHHCCCCEEEECCCCHHHCCCCCCCEEEEECCCCCCEEEHHHCC
RNKPPRGVVFMPFFDARQLVNKLILDATDPLSKETDFKKCPVKVFKAENNQ
CCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
SISRREFLKANAAVAAATAVGVTLPIKMVEAAEQPDNIKWDKAPCRFCGVGCSVLVGTK
CCCHHHHHHHHHHHHHHHHHCEEEHHHHHHHCCCCCCCCCCCCCCHHHCCCCEEEEECC
AGKVVATKGDPESPVNRGLNCIKGYFLSKIMYGKDRLTTPLLRMKDGKYHKEGEFTPISW
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHCCCCCCCCCCCCCEEEH
DMAFDTMAAKWKHSLATKGPTSVGMFGSGQWTIWEGYAASKLHKAGFLTNNIDPNARHCM
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEECCCCCCHHHHH
ASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTCRVH
HHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEE
VLSTFENRSFDLADNPMVFRPQSDLVILNFIANYIIQNNAVNTDFVTKHTKFALGVDDIG
EEEECCCCCCCCCCCCEEECCCCCEEHHHHHHHHHHCCCCCCCCCEECCCEEEECHHHCC
YGLRPDHPLEKKAKNPGNGKSTPISFDEYAKFVSTYTLEYAAKMSGVEPEKLETLAKAYA
CCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
DPNVKVMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATPGNSPFSLTGQPSACGTAREV
CCCEEEEEEEEECCCHHHCEEEECCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCHHHH
GTFAHRLPADMEVTNDKHRAITEKLWQVPEGTIPAKPGYHAVLQSRMLKDGKLNCYWTMC
HHHHHHCCCCCEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEE
TNNMQAGPNINDEIYPGFRNPENFIVVSDPYPTITAMAADLILPTAMWVEKEGAYGNAER
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHH
RTHMWHQQVKAPEGATSDLWQLVEFSKRFKVSEVWPAELIAKQPEYADKTLYEVLFANGV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCC
IDKFPTSDCKAELNDESEHFGFYLQKGIFEEYATFGRDHGHDLADFDRYHETRGLRWPVV
CCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCC
NGKETLRRFVEGSDPYVKAGEGFKFYGKPDGKAVIFALPYEPAAEEPNEEYDLWMSTGRV
CCHHHHHHHHCCCCCEEECCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
LEHWHTGSMTARVPELYRAFPDAQIFMHPEDAKARGVQRGDEVIVASPRGEVKTRVETKG
HHHHCCCCCCHHHHHHHHHCCCCEEEECCCCHHHCCCCCCCEEEEECCCCCCEEEHHHCC
RNKPPRGVVFMPFFDARQLVNKLILDATDPLSKETDFKKCPVKVFKAENNQ
CCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]