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The map label for this gene is ygfZ [C]

Identifier: 126175700

GI number: 126175700

Start: 4103143

End: 4104105

Strand: Direct

Name: ygfZ [C]

Synonym: Sbal_3503

Alternate gene names: 126175700

Gene position: 4103143-4104105 (Clockwise)

Preceding gene: 126175699

Following gene: 126175704

Centisome position: 80.02

GC content: 48.81

Gene sequence:

>963_bases
ATGACTATTTCAGTTTCTACGCCCCATTGGGATCTGGACGCTTCTTTGCCTCCACTCATGCTTGCCAACTTATCTCATAT
GGGCCTTATCAAAGTGGTTGGAGAACAAGGCCGCAGTTTCATCCATGGCCAAGTGACCACTGACATCAGCTCATTAGCCG
ATAACCAATGGCGCTGGGGCGCACATTGTGATCCAAAAGGTAAAATGCTGGCCAGTTTCAGGACCTTTGCCATCCAAGAT
GCGCTCTTTATGCTAATGCCAAAGGATGCCATCGAAGTCGATTTACCCCAGTTACAAAAATATGCCGTGTTTAGCAAAGC
CACTTTAAGCAATGCATCCGCTGAGTGGACTCTGATCGGGGTCGCCGGTGAACAAGCAAGCCTGTTCGTGAGCGAACATT
TTGGCGACATTCATCAAGAATTCACGCCCATTGAACATGGCGCGATTTTAAAAGACGCTGACCGTTTCATTTTAATGTTA
ACCCCAGAAGCTGCTGCAGCTTTAGTGGCCAAATCAAAGCTGAGCGTATTTGATGCCAGCGCTTGGCAAGCGTTAGAAAT
TATCGCAGGTTACCCCAACCTTGCCGCCAGCCATGCCAGCCAATACGTACCGCAGATGTGCAATCTACAAGCGGTTAACG
GCATTAGCTTCAACAAGGGCTGTTACATGGGGCAAGAAACCATAGCGCGGATGAAATATCGCGGCGGCAATAAACGCGCC
CTGTATATTCTGCATGGTCATACTAACCTGCAAATCAGCCTAGAGTCTGGTCTTGAAATCGCGATGGAAGATGGCTTCCG
CCGTGGCGGCCATATTATTGAGTTTGTACAGCGCGGCAACCAAGTGTTGTTAACCGCAGTGCTTGCCAACGATACCAGCA
ACGAGACTAAACTGCGTTTTGCCGACGATGAGCAATCAAGCTTAACGATTCAAGCCCTGCCCTACTCATTAGACGAAGCA
TAA

Upstream 100 bases:

>100_bases
CACATATCATTCACAGATATGTGCGCCTTATGCTTTTGAGCCAACAGGAATTCCGTTACAATCTAGCCTCTCACATCGCC
TTCATTTTCAGGATCTCGCT

Downstream 100 bases:

>100_bases
GGCTTTGATTTAATAACACGGTATAGAGATTTAAACAGAATAAAGGCGCCAATGGCGCCTTTAGCTGATCTTGTACTCAC
CCCCTCAAACACCCGAGCAC

Product: glycine cleavage T protein (aminomethyl transferase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTISVSTPHWDLDASLPPLMLANLSHMGLIKVVGEQGRSFIHGQVTTDISSLADNQWRWGAHCDPKGKMLASFRTFAIQD
ALFMLMPKDAIEVDLPQLQKYAVFSKATLSNASAEWTLIGVAGEQASLFVSEHFGDIHQEFTPIEHGAILKDADRFILML
TPEAAAALVAKSKLSVFDASAWQALEIIAGYPNLAASHASQYVPQMCNLQAVNGISFNKGCYMGQETIARMKYRGGNKRA
LYILHGHTNLQISLESGLEIAMEDGFRRGGHIIEFVQRGNQVLLTAVLANDTSNETKLRFADDEQSSLTIQALPYSLDEA

Sequences:

>Translated_320_residues
MTISVSTPHWDLDASLPPLMLANLSHMGLIKVVGEQGRSFIHGQVTTDISSLADNQWRWGAHCDPKGKMLASFRTFAIQD
ALFMLMPKDAIEVDLPQLQKYAVFSKATLSNASAEWTLIGVAGEQASLFVSEHFGDIHQEFTPIEHGAILKDADRFILML
TPEAAAALVAKSKLSVFDASAWQALEIIAGYPNLAASHASQYVPQMCNLQAVNGISFNKGCYMGQETIARMKYRGGNKRA
LYILHGHTNLQISLESGLEIAMEDGFRRGGHIIEFVQRGNQVLLTAVLANDTSNETKLRFADDEQSSLTIQALPYSLDEA
>Mature_319_residues
TISVSTPHWDLDASLPPLMLANLSHMGLIKVVGEQGRSFIHGQVTTDISSLADNQWRWGAHCDPKGKMLASFRTFAIQDA
LFMLMPKDAIEVDLPQLQKYAVFSKATLSNASAEWTLIGVAGEQASLFVSEHFGDIHQEFTPIEHGAILKDADRFILMLT
PEAAAALVAKSKLSVFDASAWQALEIIAGYPNLAASHASQYVPQMCNLQAVNGISFNKGCYMGQETIARMKYRGGNKRAL
YILHGHTNLQISLESGLEIAMEDGFRRGGHIIEFVQRGNQVLLTAVLANDTSNETKLRFADDEQSSLTIQALPYSLDEA

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=312, Percent_Identity=37.5, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 35095; Mature: 34964

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISVSTPHWDLDASLPPLMLANLSHMGLIKVVGEQGRSFIHGQVTTDISSLADNQWRWG
CEEECCCCCCCCCCCCCHHHHHCCCCCHHEEEECCCCCEEEECEEHHHHHHHCCCCCCCC
AHCDPKGKMLASFRTFAIQDALFMLMPKDAIEVDLPQLQKYAVFSKATLSNASAEWTLIG
CCCCCCCHHHHHHHHHHHHHHHHEECCCCHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
VAGEQASLFVSEHFGDIHQEFTPIEHGAILKDADRFILMLTPEAAAALVAKSKLSVFDAS
ECCCCCEEEHHHHHHHHHHHCCCCCCCCEEECCCCEEEEECCHHHHHHHHHHHHHEEECC
AWQALEIIAGYPNLAASHASQYVPQMCNLQAVNGISFNKGCYMGQETIARMKYRGGNKRA
HHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEE
LYILHGHTNLQISLESGLEIAMEDGFRRGGHIIEFVQRGNQVLLTAVLANDTSNETKLRF
EEEEECCCEEEEEECCCCEEEECCCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCCEEEE
ADDEQSSLTIQALPYSLDEA
ECCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
TISVSTPHWDLDASLPPLMLANLSHMGLIKVVGEQGRSFIHGQVTTDISSLADNQWRWG
EEECCCCCCCCCCCCCHHHHHCCCCCHHEEEECCCCCEEEECEEHHHHHHHCCCCCCCC
AHCDPKGKMLASFRTFAIQDALFMLMPKDAIEVDLPQLQKYAVFSKATLSNASAEWTLIG
CCCCCCCHHHHHHHHHHHHHHHHEECCCCHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
VAGEQASLFVSEHFGDIHQEFTPIEHGAILKDADRFILMLTPEAAAALVAKSKLSVFDAS
ECCCCCEEEHHHHHHHHHHHCCCCCCCCEEECCCCEEEEECCHHHHHHHHHHHHHEEECC
AWQALEIIAGYPNLAASHASQYVPQMCNLQAVNGISFNKGCYMGQETIARMKYRGGNKRA
HHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEE
LYILHGHTNLQISLESGLEIAMEDGFRRGGHIIEFVQRGNQVLLTAVLANDTSNETKLRF
EEEEECCCEEEEEECCCCEEEECCCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCCEEEE
ADDEQSSLTIQALPYSLDEA
ECCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]