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The map label for this gene is nfnB [H]

Identifier: 126175593

GI number: 126175593

Start: 3977041

End: 3977694

Strand: Direct

Name: nfnB [H]

Synonym: Sbal_3396

Alternate gene names: 126175593

Gene position: 3977041-3977694 (Clockwise)

Preceding gene: 126175591

Following gene: 126175594

Centisome position: 77.56

GC content: 43.12

Gene sequence:

>654_bases
ATGAGTGATTTAAGTTTCTTCGCCAAAAAACGTTACACCACTAAAGCCTTTGATCCAACAAAGACGATTCCAGCGGAAAA
AATTGCAGACATCAAAACCCTGCTACAATTCAGCCCTTCGTCAGTTAACTCACAGCCTTGGCATTTTGTATTAGCAAGCA
CGGCCGAAGGTAAAGCTTTAATCGCCCAAGCAACCGAGAATTATGCGTTTAATACCCAAAAAATCCTCAATGCTTCCCAT
GTGGTGGTGCTCTGCACTCGCACTCAATTAGATGAAGCGTATTTGCTGCAAATCCTTGAACAAGAGGAAAAAGATGGCCG
CTTAGCTAATGAGGAAGCAAAACAAGCCCAGCATAATGGCCGTTCTTTCTTCGCCAACATGCATAAATTTGAGATGAAAG
ATGCCCAACATTGGATGGAGAAACAGGTCTATTTAGCGCTCGGCACCTTAATGCTCGGCGCAAGCGTGTTAGATATCGAT
ACTTGTCCGATTGAAGGTTTTGATGCCACTAAAATGAATCAAGTCTTAGGCTTAAGAGAGCAAGGTCTATGCGCCTGCGT
AGTTGTCGCTATGGGTTATCACGCAGAGGATGATTTCAACGCTAAATTGCCTAAGTCTCGCTTAGATCAAGAGGTGATTT
TTACTGAAATATAG

Upstream 100 bases:

>100_bases
TTTTTGAAACTAATACCTGTCTTGATCACATTGTTTTTTACTTGAACCTGCTTAAAATGCTGCCATTGAATTTGTATTCC
TGATGATTTGGAGCTGTTAC

Downstream 100 bases:

>100_bases
CCCTCATCTTAAGTGAATAATAAACATGCCCTTGATTTAATGTGATATAAACAAGGGCATAATTTTTTGCACCCTAGCGC
TCTTCGCCGACAATATCCTA

Product: dihydropteridine reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSDLSFFAKKRYTTKAFDPTKTIPAEKIADIKTLLQFSPSSVNSQPWHFVLASTAEGKALIAQATENYAFNTQKILNASH
VVVLCTRTQLDEAYLLQILEQEEKDGRLANEEAKQAQHNGRSFFANMHKFEMKDAQHWMEKQVYLALGTLMLGASVLDID
TCPIEGFDATKMNQVLGLREQGLCACVVVAMGYHAEDDFNAKLPKSRLDQEVIFTEI

Sequences:

>Translated_217_residues
MSDLSFFAKKRYTTKAFDPTKTIPAEKIADIKTLLQFSPSSVNSQPWHFVLASTAEGKALIAQATENYAFNTQKILNASH
VVVLCTRTQLDEAYLLQILEQEEKDGRLANEEAKQAQHNGRSFFANMHKFEMKDAQHWMEKQVYLALGTLMLGASVLDID
TCPIEGFDATKMNQVLGLREQGLCACVVVAMGYHAEDDFNAKLPKSRLDQEVIFTEI
>Mature_216_residues
SDLSFFAKKRYTTKAFDPTKTIPAEKIADIKTLLQFSPSSVNSQPWHFVLASTAEGKALIAQATENYAFNTQKILNASHV
VVLCTRTQLDEAYLLQILEQEEKDGRLANEEAKQAQHNGRSFFANMHKFEMKDAQHWMEKQVYLALGTLMLGASVLDIDT
CPIEGFDATKMNQVLGLREQGLCACVVVAMGYHAEDDFNAKLPKSRLDQEVIFTEI

Specific function: Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=216, Percent_Identity=51.8518518518518, Blast_Score=243, Evalue=9e-66,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.-; 1.5.1.34

Molecular weight: Translated: 24368; Mature: 24237

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLSFFAKKRYTTKAFDPTKTIPAEKIADIKTLLQFSPSSVNSQPWHFVLASTAEGKAL
CCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEE
IAQATENYAFNTQKILNASHVVVLCTRTQLDEAYLLQILEQEEKDGRLANEEAKQAQHNG
EEEHHCCCCCCHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
RSFFANMHKFEMKDAQHWMEKQVYLALGTLMLGASVLDIDTCPIEGFDATKMNQVLGLRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCCHHHHHHHHCCCC
QGLCACVVVAMGYHAEDDFNAKLPKSRLDQEVIFTEI
CCHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHEECC
>Mature Secondary Structure 
SDLSFFAKKRYTTKAFDPTKTIPAEKIADIKTLLQFSPSSVNSQPWHFVLASTAEGKAL
CCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEE
IAQATENYAFNTQKILNASHVVVLCTRTQLDEAYLLQILEQEEKDGRLANEEAKQAQHNG
EEEHHCCCCCCHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
RSFFANMHKFEMKDAQHWMEKQVYLALGTLMLGASVLDIDTCPIEGFDATKMNQVLGLRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCCHHHHHHHHCCCC
QGLCACVVVAMGYHAEDDFNAKLPKSRLDQEVIFTEI
CCHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2179862; 11677609 [H]