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The map label for this gene is pcm

Identifier: 126175322

GI number: 126175322

Start: 3657040

End: 3657675

Strand: Reverse

Name: pcm

Synonym: Sbal_3121

Alternate gene names: 126175322

Gene position: 3657675-3657040 (Counterclockwise)

Preceding gene: 126175323

Following gene: 126175321

Centisome position: 71.34

GC content: 47.8

Gene sequence:

>636_bases
ATGACTCGAGTTGCCTTAACATCGGCGGTGAACTTAGCTAAAAAGCTTCATGATGCGGGGATCCGCAATCAAGCCGTGTT
AAAGGCGATATCGCATACGCCGCGGGAAATGTTTCTCGATAATGCGCTAGCCCATAAAGCCTATGAAAATACCGCTTTAC
CGATTGGCCAAGGGCAAACCATCTCGCAGCCTTATATCGTCGCCCGTATGACCGAACTCTTACTGCATAAGATGCCCCAG
CGCGTATTAGAAGTGGGTACAGGATCGGGTTATCAGGCGGCAATTTTGGCGCAATTAGTGCCGCAGCTTTGTACTATCGA
GCGAATAAAAGGCTTACAAATTCAAGCGAGACAGCGCTTAAAACGATTAGATTTACATAATGTGTCGTTTAAATATGGTG
ATGGCTGGTTAGGTTGGGCGAATCGTAGCCCGTTCGATGCCATTATGGTGACCGCTGCCGCTTCGACTATACCTGAGGCC
TTACTGTCTCAATTAGCCGAGGGCGGAGTGTTAGTTCTGCCAGTCGGCGAAGATACCCAGCAATTGATGCGAATCACTCG
TACTGCGGACCGCTTCAGTTCGGAAACAATTGAAACGGTTAAATTTGTCCCTTTGATTAATGGAGAGTTAGCTTAA

Upstream 100 bases:

>100_bases
TTCATGCTATCGCCAATGGCTATGTGTCAATTACGCCTTTGACGGTTGATTTAACTGCCTACGGACAACTAACGGCATTG
CAAAATTGGGTAGATAAAAT

Downstream 100 bases:

>100_bases
CCTTGTGTTGTGCTACTGTCTCCACTGAACAACTTGTCTTGCAACGAGGGCATTGTTTTCTCATCATAAACAGTAATACA
GCCTCACCAAGGAGTTTTTA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTRVALTSAVNLAKKLHDAGIRNQAVLKAISHTPREMFLDNALAHKAYENTALPIGQGQTISQPYIVARMTELLLHKMPQ
RVLEVGTGSGYQAAILAQLVPQLCTIERIKGLQIQARQRLKRLDLHNVSFKYGDGWLGWANRSPFDAIMVTAAASTIPEA
LLSQLAEGGVLVLPVGEDTQQLMRITRTADRFSSETIETVKFVPLINGELA

Sequences:

>Translated_211_residues
MTRVALTSAVNLAKKLHDAGIRNQAVLKAISHTPREMFLDNALAHKAYENTALPIGQGQTISQPYIVARMTELLLHKMPQ
RVLEVGTGSGYQAAILAQLVPQLCTIERIKGLQIQARQRLKRLDLHNVSFKYGDGWLGWANRSPFDAIMVTAAASTIPEA
LLSQLAEGGVLVLPVGEDTQQLMRITRTADRFSSETIETVKFVPLINGELA
>Mature_210_residues
TRVALTSAVNLAKKLHDAGIRNQAVLKAISHTPREMFLDNALAHKAYENTALPIGQGQTISQPYIVARMTELLLHKMPQR
VLEVGTGSGYQAAILAQLVPQLCTIERIKGLQIQARQRLKRLDLHNVSFKYGDGWLGWANRSPFDAIMVTAAASTIPEAL
LSQLAEGGVLVLPVGEDTQQLMRITRTADRFSSETIETVKFVPLINGELA

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=193, Percent_Identity=33.6787564766839, Blast_Score=81, Evalue=5e-16,
Organism=Escherichia coli, GI1789100, Length=200, Percent_Identity=62, Blast_Score=256, Evalue=8e-70,
Organism=Caenorhabditis elegans, GI71983477, Length=178, Percent_Identity=32.0224719101124, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI193207222, Length=143, Percent_Identity=33.5664335664336, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17981723, Length=216, Percent_Identity=32.8703703703704, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_SHEB2 (B8E8T7)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002357176.1
- ProteinModelPortal:   B8E8T7
- SMR:   B8E8T7
- GeneID:   7088015
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_1243
- HOGENOM:   HBG699907
- OMA:   YSVERIR
- ProtClustDB:   CLSK907169
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23151; Mature: 23020

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 62-62

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVALTSAVNLAKKLHDAGIRNQAVLKAISHTPREMFLDNALAHKAYENTALPIGQGQT
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
ISQPYIVARMTELLLHKMPQRVLEVGTGSGYQAAILAQLVPQLCTIERIKGLQIQARQRL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KRLDLHNVSFKYGDGWLGWANRSPFDAIMVTAAASTIPEALLSQLAEGGVLVLPVGEDTQ
HHHCCCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHH
QLMRITRTADRFSSETIETVKFVPLINGELA
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
>Mature Secondary Structure 
TRVALTSAVNLAKKLHDAGIRNQAVLKAISHTPREMFLDNALAHKAYENTALPIGQGQT
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
ISQPYIVARMTELLLHKMPQRVLEVGTGSGYQAAILAQLVPQLCTIERIKGLQIQARQRL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KRLDLHNVSFKYGDGWLGWANRSPFDAIMVTAAASTIPEALLSQLAEGGVLVLPVGEDTQ
HHHCCCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHH
QLMRITRTADRFSSETIETVKFVPLINGELA
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA