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The map label for this gene is fliD [H]

Identifier: 126175135

GI number: 126175135

Start: 3447101

End: 3448471

Strand: Reverse

Name: fliD [H]

Synonym: Sbal_2933

Alternate gene names: 126175135

Gene position: 3448471-3447101 (Counterclockwise)

Preceding gene: 126175136

Following gene: 126175134

Centisome position: 67.26

GC content: 38.8

Gene sequence:

>1371_bases
ATGGCATTAACAGCGACAGGCATAGGTTCTGGCCTTAAAATTAATGAAATTGTGCAAGTTTTAGTCGATGCTGAAAAAAA
GCCGAAAGAAGCGATGTTTAATAAAAAGGAAGACAGCATTAAAGCAAAGGTTTCTGCCATGGGAACTTTAAAAAGTGCGT
TGTCTACGTTTCAAGATGCCTTGAAAAAACTTCAAACTGGCGATGCCCTTAATCAACGAAAAATCACTGTCAGTAATGAA
ACCTATTTAACTGCCACAGCGGATAAAACGGCACAAGCGGGTAGTTATGGTATTAAAGTTGAGCAGCTTGCCGTGAATCA
TAAAGTTGCTGGGATAAATGTCGCAGATCCTACATTACCTGTCGGTGAAGGAAGTTTAGATTTTGGCATTAATGGAAAGA
ATTTTTCTATTGATGTTTCCGCTACCGATTCAATTGCTGCTATTGCTAAAAAAGTCAATGAGTCATCGGATAATGTAGGT
GTAACTGCAACTGTTATTACCAGTGATGCTGGCAGTCGGTTGATTTTTAGCTCTAATAAATCAGGTGAAGATAATCAAAT
CAGTATTACAGCTAATGACACGTCTGGTACTGGTCTGAATGATATGTTTGGCGCAGGAAATATCACCTCACTTCAAGATG
CTAAAAATGCAATTGTTTATATTGATAATCAGAAGGTTACCTCACAAACAAATGAGGTTAAAAATGCTATCGCAGGTGTG
ACGTTAAACTTAACTGATGCCGATATTTCTAAGACGACGACACTAAAAATCGAACAAGATGATGGTGCAGTAAAGACCAA
TGTTAAGGCTTTTGTTGACGCTTATAATAGTTTAGCCACTGCAGTTGATAAGATGTCGTCCTATGATAAGGACAAAAAAA
CCGCAGCTGCATTACAGGGCGACTCTATGGTTCGCTCGATAGAATCTCAATTACGAAGCATATCGAGTGAGCGAATTGAT
ACTGATGGTGGTAATTCGATTGCCTTATATGACATAGGCATTAAAACAGATAGATATGGCAAGTTATCTGTTGATGATAC
TAAACTTGATAAAGTGCTTTCAGAGGATATGTCTAGTGTTGAGGCGTTATTTTCAACGCCAGATAAAGGGCTTGCCAATC
GTTTTTATGATCTTGCTGAAAGCTACGTTAAGTCACGTGGCCTAATTGCGTCTCGTCAAACAGGATATACCAATCAGCAA
AGTCAAATCACAGATCAAAGAGAAGCCTTTACGCTTAAAATGGATCAGTTAACAGCGCGTTTAACTAAGCAATTTAATGC
GATGGATCTTATCGTTGGCAAACTCAATCAACAGAGTAGTGGTCTCGTTGATCGTATAAATTCACTTCCTGGTGCGGTTA
GGAAGGGGTAA

Upstream 100 bases:

>100_bases
GCTGAAAAATTATCTGAAGTCACTGGGCTTTTGATGAAAACAGAGGCATAGATCTGTTTATCATTACTATATAGTGATTG
TTGAAGAGTGAGAGGTTTAT

Downstream 100 bases:

>100_bases
TCAGTTTTAAATGAGTGAGGAAAGGAAGTGTCAGTGAAACAACTCGATGAGCTTAATGTCCTTTTGAGTGAAACCTTAAA
TAAATTGACGCAGATCCCTG

Product: flagellar hook-associated 2 domain-containing protein

Products: NA

Alternate protein names: HAP2; Filament cap protein [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MALTATGIGSGLKINEIVQVLVDAEKKPKEAMFNKKEDSIKAKVSAMGTLKSALSTFQDALKKLQTGDALNQRKITVSNE
TYLTATADKTAQAGSYGIKVEQLAVNHKVAGINVADPTLPVGEGSLDFGINGKNFSIDVSATDSIAAIAKKVNESSDNVG
VTATVITSDAGSRLIFSSNKSGEDNQISITANDTSGTGLNDMFGAGNITSLQDAKNAIVYIDNQKVTSQTNEVKNAIAGV
TLNLTDADISKTTTLKIEQDDGAVKTNVKAFVDAYNSLATAVDKMSSYDKDKKTAAALQGDSMVRSIESQLRSISSERID
TDGGNSIALYDIGIKTDRYGKLSVDDTKLDKVLSEDMSSVEALFSTPDKGLANRFYDLAESYVKSRGLIASRQTGYTNQQ
SQITDQREAFTLKMDQLTARLTKQFNAMDLIVGKLNQQSSGLVDRINSLPGAVRKG

Sequences:

>Translated_456_residues
MALTATGIGSGLKINEIVQVLVDAEKKPKEAMFNKKEDSIKAKVSAMGTLKSALSTFQDALKKLQTGDALNQRKITVSNE
TYLTATADKTAQAGSYGIKVEQLAVNHKVAGINVADPTLPVGEGSLDFGINGKNFSIDVSATDSIAAIAKKVNESSDNVG
VTATVITSDAGSRLIFSSNKSGEDNQISITANDTSGTGLNDMFGAGNITSLQDAKNAIVYIDNQKVTSQTNEVKNAIAGV
TLNLTDADISKTTTLKIEQDDGAVKTNVKAFVDAYNSLATAVDKMSSYDKDKKTAAALQGDSMVRSIESQLRSISSERID
TDGGNSIALYDIGIKTDRYGKLSVDDTKLDKVLSEDMSSVEALFSTPDKGLANRFYDLAESYVKSRGLIASRQTGYTNQQ
SQITDQREAFTLKMDQLTARLTKQFNAMDLIVGKLNQQSSGLVDRINSLPGAVRKG
>Mature_455_residues
ALTATGIGSGLKINEIVQVLVDAEKKPKEAMFNKKEDSIKAKVSAMGTLKSALSTFQDALKKLQTGDALNQRKITVSNET
YLTATADKTAQAGSYGIKVEQLAVNHKVAGINVADPTLPVGEGSLDFGINGKNFSIDVSATDSIAAIAKKVNESSDNVGV
TATVITSDAGSRLIFSSNKSGEDNQISITANDTSGTGLNDMFGAGNITSLQDAKNAIVYIDNQKVTSQTNEVKNAIAGVT
LNLTDADISKTTTLKIEQDDGAVKTNVKAFVDAYNSLATAVDKMSSYDKDKKTAAALQGDSMVRSIESQLRSISSERIDT
DGGNSIALYDIGIKTDRYGKLSVDDTKLDKVLSEDMSSVEALFSTPDKGLANRFYDLAESYVKSRGLIASRQTGYTNQQS
QITDQREAFTLKMDQLTARLTKQFNAMDLIVGKLNQQSSGLVDRINSLPGAVRKG

Specific function: Required for the morphogenesis and for the elongation of the flagellar filament by facilitating polymerization of the flagellin monomers at the tip of growing filament. Forms a capping structure, which prevents flagellin subunits (transported through the

COG id: COG1345

COG function: function code N; Flagellar capping protein

Gene ontology:

Cell location: Secreted. Bacterial flagellum [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliD family [H]

Homologues:

Organism=Escherichia coli, GI1788233, Length=471, Percent_Identity=30.3609341825902, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010810
- InterPro:   IPR010809
- InterPro:   IPR003481 [H]

Pfam domain/function: PF07196 Flagellin_IN; PF07195 FliD_C; PF02465 FliD_N [H]

EC number: NA

Molecular weight: Translated: 48596; Mature: 48465

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTATGIGSGLKINEIVQVLVDAEKKPKEAMFNKKEDSIKAKVSAMGTLKSALSTFQDA
CEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LKKLQTGDALNQRKITVSNETYLTATADKTAQAGSYGIKVEQLAVNHKVAGINVADPTLP
HHHHHCCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCEEEEEEECCEEEEEEECCCCCC
VGEGSLDFGINGKNFSIDVSATDSIAAIAKKVNESSDNVGVTATVITSDAGSRLIFSSNK
CCCCCEEECCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEECCC
SGEDNQISITANDTSGTGLNDMFGAGNITSLQDAKNAIVYIDNQKVTSQTNEVKNAIAGV
CCCCCEEEEEECCCCCCCCHHHCCCCCCCCHHCCCCEEEEECCCCHHHHHHHHHHHHCCE
TLNLTDADISKTTTLKIEQDDGAVKTNVKAFVDAYNSLATAVDKMSSYDKDKKTAAALQG
EEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
DSMVRSIESQLRSISSERIDTDGGNSIALYDIGIKTDRYGKLSVDDTKLDKVLSEDMSSV
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
EALFSTPDKGLANRFYDLAESYVKSRGLIASRQTGYTNQQSQITDQREAFTLKMDQLTAR
HHHHCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHCCCHHHEEEEHHHHHHH
LTKQFNAMDLIVGKLNQQSSGLVDRINSLPGAVRKG
HHHHCCHHHHHHHCCCCCCCHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
ALTATGIGSGLKINEIVQVLVDAEKKPKEAMFNKKEDSIKAKVSAMGTLKSALSTFQDA
EEEECCCCCCCCHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LKKLQTGDALNQRKITVSNETYLTATADKTAQAGSYGIKVEQLAVNHKVAGINVADPTLP
HHHHHCCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCEEEEEEECCEEEEEEECCCCCC
VGEGSLDFGINGKNFSIDVSATDSIAAIAKKVNESSDNVGVTATVITSDAGSRLIFSSNK
CCCCCEEECCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEECCC
SGEDNQISITANDTSGTGLNDMFGAGNITSLQDAKNAIVYIDNQKVTSQTNEVKNAIAGV
CCCCCEEEEEECCCCCCCCHHHCCCCCCCCHHCCCCEEEEECCCCHHHHHHHHHHHHCCE
TLNLTDADISKTTTLKIEQDDGAVKTNVKAFVDAYNSLATAVDKMSSYDKDKKTAAALQG
EEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
DSMVRSIESQLRSISSERIDTDGGNSIALYDIGIKTDRYGKLSVDDTKLDKVLSEDMSSV
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
EALFSTPDKGLANRFYDLAESYVKSRGLIASRQTGYTNQQSQITDQREAFTLKMDQLTAR
HHHHCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHCCCHHHEEEEHHHHHHH
LTKQFNAMDLIVGKLNQQSSGLVDRINSLPGAVRKG
HHHHCCHHHHHHHCCCCCCCHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9488388; 10678962 [H]