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The map label for this gene is pyrF [H]

Identifier: 126174295

GI number: 126174295

Start: 2406768

End: 2407463

Strand: Direct

Name: pyrF [H]

Synonym: Sbal_2074

Alternate gene names: 126174295

Gene position: 2406768-2407463 (Clockwise)

Preceding gene: 126174294

Following gene: 126174296

Centisome position: 46.94

GC content: 49.57

Gene sequence:

>696_bases
ATGACGACTAAACCTATCCTCGTAGCGCTTGATTACGATAACAAAAACCACGCTTTACAACTGATAGAAAAACTCGATCC
CAACATGTGCCGCCTTAAAGTTGGCAAAGAAATGTTTACCTTGTTTGGCCCGCAACTGGTGAAAGATATCCATGATCGTG
GCTTTGATTTATTCCTCGATTTGAAATTTCACGATATTCCCAATACTGTCGCCAAAGCCGTGGCCGCTGCCGCAGAGTTG
GGTGTATGGATGACGAACGTGCATGCCAGTGGTGGTTTAGCCATGATGGAAGCGGCTAAAAAGGCCTTGCTGCCATATGG
CGACAAAGCGCCTATGCTGATCGCTGTGACGGTATTAACCTCCATGAGTGATGATGATCTTAAATTACTCGGCATCAATG
TGCCTGCATTTGAACATGTTCAGCGTTTGGCGAAGCTCACGCAGCAAGCGGGACTTGATGGTGTGGTGTGTTCCGCCCAA
GAAGCGACAGCGTTGAAATCGCTGTTAGGTCAGGATTTCAAACTCGTTACGCCGGGGATCCGCCCAGTAGGTAGCGATGC
AGGCGATCAACACCGGGTGATGACGCCGCCTCAAGCGATTGCAGCGGGTTCAGATTACCTAGTGATTGGCCGTCCAATCA
CCAAAGCTGCCGATCCATTAGCGGCATTGCAAGCGATTCACCAATCATTGGCATAG

Upstream 100 bases:

>100_bases
CCTTCCTGTAAAAATTGGGGTAGTATCAAGCGGATCAGAGGCTTAGACGGCGAGTAAATAAGATAATATCTATAGTTAGT
GTAACTCTGTTGGAGAGATG

Downstream 100 bases:

>100_bases
ACGTTTCAATAGCCGTCTCAATCGCGGTTTGACCGTCGTTTGAATAAAAAAGGCTTGAAATCGCTCGTTAGTAACCTTGA
TATGATGGATAAAAATTGAA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTTKPILVALDYDNKNHALQLIEKLDPNMCRLKVGKEMFTLFGPQLVKDIHDRGFDLFLDLKFHDIPNTVAKAVAAAAEL
GVWMTNVHASGGLAMMEAAKKALLPYGDKAPMLIAVTVLTSMSDDDLKLLGINVPAFEHVQRLAKLTQQAGLDGVVCSAQ
EATALKSLLGQDFKLVTPGIRPVGSDAGDQHRVMTPPQAIAAGSDYLVIGRPITKAADPLAALQAIHQSLA

Sequences:

>Translated_231_residues
MTTKPILVALDYDNKNHALQLIEKLDPNMCRLKVGKEMFTLFGPQLVKDIHDRGFDLFLDLKFHDIPNTVAKAVAAAAEL
GVWMTNVHASGGLAMMEAAKKALLPYGDKAPMLIAVTVLTSMSDDDLKLLGINVPAFEHVQRLAKLTQQAGLDGVVCSAQ
EATALKSLLGQDFKLVTPGIRPVGSDAGDQHRVMTPPQAIAAGSDYLVIGRPITKAADPLAALQAIHQSLA
>Mature_230_residues
TTKPILVALDYDNKNHALQLIEKLDPNMCRLKVGKEMFTLFGPQLVKDIHDRGFDLFLDLKFHDIPNTVAKAVAAAAELG
VWMTNVHASGGLAMMEAAKKALLPYGDKAPMLIAVTVLTSMSDDDLKLLGINVPAFEHVQRLAKLTQQAGLDGVVCSAQE
ATALKSLLGQDFKLVTPGIRPVGSDAGDQHRVMTPPQAIAAGSDYLVIGRPITKAADPLAALQAIHQSLA

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=230, Percent_Identity=64.7826086956522, Blast_Score=309, Evalue=8e-86,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 24726; Mature: 24595

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00599 AA_TRANSFER_CLASS_2 ; PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKPILVALDYDNKNHALQLIEKLDPNMCRLKVGKEMFTLFGPQLVKDIHDRGFDLFLD
CCCCCEEEEEECCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHCHHHHHHHHHCCCEEEEE
LKFHDIPNTVAKAVAAAAELGVWMTNVHASGGLAMMEAAKKALLPYGDKAPMLIAVTVLT
EEECCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHH
SMSDDDLKLLGINVPAFEHVQRLAKLTQQAGLDGVVCSAQEATALKSLLGQDFKLVTPGI
CCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCEEECCCC
RPVGSDAGDQHRVMTPPQAIAAGSDYLVIGRPITKAADPLAALQAIHQSLA
CCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TTKPILVALDYDNKNHALQLIEKLDPNMCRLKVGKEMFTLFGPQLVKDIHDRGFDLFLD
CCCCEEEEEECCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHCHHHHHHHHHCCCEEEEE
LKFHDIPNTVAKAVAAAAELGVWMTNVHASGGLAMMEAAKKALLPYGDKAPMLIAVTVLT
EEECCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHH
SMSDDDLKLLGINVPAFEHVQRLAKLTQQAGLDGVVCSAQEATALKSLLGQDFKLVTPGI
CCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCEEECCCC
RPVGSDAGDQHRVMTPPQAIAAGSDYLVIGRPITKAADPLAALQAIHQSLA
CCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]