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The map label for this gene is cphE [H]

Identifier: 126174211

GI number: 126174211

Start: 2306198

End: 2307823

Strand: Direct

Name: cphE [H]

Synonym: Sbal_1987

Alternate gene names: 126174211

Gene position: 2306198-2307823 (Clockwise)

Preceding gene: 126174206

Following gene: 126174214

Centisome position: 44.98

GC content: 45.57

Gene sequence:

>1626_bases
ATGGAAATCTGCAGCTCTCAAGCTCCCGCAAATTGCCAATCGGAAGTCAAATTCTCAGCCGATGCCAAACGCGAAACACG
TTATGTATTCAACGTTTCGTGGCTTGAACGTGCACTTAAACACCCAAGATTGCAGTCGTTAAATGAGCAACAAAAAGTGA
TATTACGACAAGCGGCCAAAGCACTTTCTCCTGTCAGTAATAGTCTTGAAAATAGCGATGTTGAAATTGGCCGTTCACTT
GGCCGTTTACATAGTGACAGCTTAACCTTGGCAAAGCTTGAACAAGCCATTGCAAAGATAGATAAGCAATTTCTGGCGCA
TTTGAGCGACCAACAATATTACGCACTGCTAGATTTATTAGAAGAAAAGCAAATGCTTGCTGACGGCACGCTGTTACAAG
AACAAGTGTTACTTGATTCCACAACCAATCCCCATGCCGCGACGCTTTATCAATTATTTACGCAGCAAGCGTTGGCAAAG
CGCCAGCAAAGCAACCCCAATGCCAAACGTCCACTGATCGCCGTTGTGACCGCATCTGCGCGCGATCCCTTTGAAGCGGC
CCATTACTATGTCAGCGTATTTGAGCAAATAGGGGCCGAGGTTATTTGGCTGCCTTTAGATGCAGCACTGCAAGCGGGTA
TCGCTACCCGTCAATGTGAGCAACTCGAAATGTTTAGACAGCAAATACAAGGTAATACGGATAGGGCGAGGCTTTATCCA
AAGCAAGTTGAGCTACAAAAGGATTTCTGTGCCAGCCCCATTAAACTCTATCAATTGCTTAATCAAATCGACGGACTCTT
TTTAAATGGCGGCGATCAGAGTTTGACTTTAAAGGCTTGGACGACGCCGGATGAATCACCCAGTGAAGCATTAAACATCA
TCAAGAAACGTCTTTCCAAACAAGAAATTGTTATCGGTGGCACCAGCGCTGGCACTGCTGTGATGGCTGATAAAAATATG
ATCACCGGTGGCACAAGTGAGGGGGCATTGAGTTTTGGCATTTTCGCGACCGAGGCGCCAAGTGAGCGTTGTGAAGTCGC
TAATTGTGATGCCTCAGTTCCTGCAACGGCGGTCACTTATCGCGAGCAAGGTGGGCTTGGATTTTTCCCCTTTGGTATAC
CCGACACACACTTTTCACAACGAGACCGTTATTTAAGACTCTTGCTCCTTAGCGCCAATGCCGATAGCAAAATGGCATTT
GGGGTCGATGAAAATACAGCATTATTGGTCGATTTATCACATCGTTCGCTCTCAGTGGTCGGTGAGCATGGAGTATGGGC
ATTAGCGCAAGTTGAGCGATTGAAACAAAGCCAAGCTAACTCTGAAATAAATGCGATTTCGCACTATCTCACTCCGGGTT
CCTATGCTGAAATTGACGCCACTGGTGAGCATTTGACTCATATCCGCCTAGCTCAGACTGCCAAATTGACGCCAATAATG
AGTAAGCAGAGTGACTTTTTTACTTGGGTCAGCAATGCGTGCCGGCAAGGTATATATTCAACATCACTGAGTGATAAGTA
CGAATTAACTATTTCTCCAATGGCGGCATTAAGCTGCCAAGGTATTTTAAATCAAGAACACGGATATCAATCGATGGGGT
TAACACTAAAAGCGTTAGAACAATGA

Upstream 100 bases:

>100_bases
GCTATTTATCGCCCCAAGCGTGCAGTCAGCGCCAGCGAAAACCCTGAGTTTATCGATACCCACTCATCAACAATTTGATC
TTATGCTGGTGGGCGGAGGG

Downstream 100 bases:

>100_bases
CAGTGAGTTGAATTAATTGTAGTGGTATGGCTATGATATCAATAGGATAGGCTAAATTGATTAAGATTGAGTGTATATTA
GATAGATTTTAAAACGTGAG

Product: cyanophycinase-related exopeptidase-like protein

Products: NA

Alternate protein names: Extracellular CGPase; CPHEpa; Extracellular cyanophycinase [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MEICSSQAPANCQSEVKFSADAKRETRYVFNVSWLERALKHPRLQSLNEQQKVILRQAAKALSPVSNSLENSDVEIGRSL
GRLHSDSLTLAKLEQAIAKIDKQFLAHLSDQQYYALLDLLEEKQMLADGTLLQEQVLLDSTTNPHAATLYQLFTQQALAK
RQQSNPNAKRPLIAVVTASARDPFEAAHYYVSVFEQIGAEVIWLPLDAALQAGIATRQCEQLEMFRQQIQGNTDRARLYP
KQVELQKDFCASPIKLYQLLNQIDGLFLNGGDQSLTLKAWTTPDESPSEALNIIKKRLSKQEIVIGGTSAGTAVMADKNM
ITGGTSEGALSFGIFATEAPSERCEVANCDASVPATAVTYREQGGLGFFPFGIPDTHFSQRDRYLRLLLLSANADSKMAF
GVDENTALLVDLSHRSLSVVGEHGVWALAQVERLKQSQANSEINAISHYLTPGSYAEIDATGEHLTHIRLAQTAKLTPIM
SKQSDFFTWVSNACRQGIYSTSLSDKYELTISPMAALSCQGILNQEHGYQSMGLTLKALEQ

Sequences:

>Translated_541_residues
MEICSSQAPANCQSEVKFSADAKRETRYVFNVSWLERALKHPRLQSLNEQQKVILRQAAKALSPVSNSLENSDVEIGRSL
GRLHSDSLTLAKLEQAIAKIDKQFLAHLSDQQYYALLDLLEEKQMLADGTLLQEQVLLDSTTNPHAATLYQLFTQQALAK
RQQSNPNAKRPLIAVVTASARDPFEAAHYYVSVFEQIGAEVIWLPLDAALQAGIATRQCEQLEMFRQQIQGNTDRARLYP
KQVELQKDFCASPIKLYQLLNQIDGLFLNGGDQSLTLKAWTTPDESPSEALNIIKKRLSKQEIVIGGTSAGTAVMADKNM
ITGGTSEGALSFGIFATEAPSERCEVANCDASVPATAVTYREQGGLGFFPFGIPDTHFSQRDRYLRLLLLSANADSKMAF
GVDENTALLVDLSHRSLSVVGEHGVWALAQVERLKQSQANSEINAISHYLTPGSYAEIDATGEHLTHIRLAQTAKLTPIM
SKQSDFFTWVSNACRQGIYSTSLSDKYELTISPMAALSCQGILNQEHGYQSMGLTLKALEQ
>Mature_541_residues
MEICSSQAPANCQSEVKFSADAKRETRYVFNVSWLERALKHPRLQSLNEQQKVILRQAAKALSPVSNSLENSDVEIGRSL
GRLHSDSLTLAKLEQAIAKIDKQFLAHLSDQQYYALLDLLEEKQMLADGTLLQEQVLLDSTTNPHAATLYQLFTQQALAK
RQQSNPNAKRPLIAVVTASARDPFEAAHYYVSVFEQIGAEVIWLPLDAALQAGIATRQCEQLEMFRQQIQGNTDRARLYP
KQVELQKDFCASPIKLYQLLNQIDGLFLNGGDQSLTLKAWTTPDESPSEALNIIKKRLSKQEIVIGGTSAGTAVMADKNM
ITGGTSEGALSFGIFATEAPSERCEVANCDASVPATAVTYREQGGLGFFPFGIPDTHFSQRDRYLRLLLLSANADSKMAF
GVDENTALLVDLSHRSLSVVGEHGVWALAQVERLKQSQANSEINAISHYLTPGSYAEIDATGEHLTHIRLAQTAKLTPIM
SKQSDFFTWVSNACRQGIYSTSLSDKYELTISPMAALSCQGILNQEHGYQSMGLTLKALEQ

Specific function: Exopeptidase that catalyzes the hydrolytic cleavage of multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water- insoluble reserve polymer) into aspartate-arginine dipeptides [H]

COG id: COG4242

COG function: function code QP; Cyanophycinase and related exopeptidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S51 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005320
- InterPro:   IPR011811 [H]

Pfam domain/function: PF03575 Peptidase_S51 [H]

EC number: =3.4.15.6 [H]

Molecular weight: Translated: 59632; Mature: 59632

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEICSSQAPANCQSEVKFSADAKRETRYVFNVSWLERALKHPRLQSLNEQQKVILRQAAK
CCCCCCCCCCCCHHCCEECCCCCCCCEEEEEHHHHHHHHCCCHHHCCCHHHHHHHHHHHH
ALSPVSNSLENSDVEIGRSLGRLHSDSLTLAKLEQAIAKIDKQFLAHLSDQQYYALLDLL
HHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EEKQMLADGTLLQEQVLLDSTTNPHAATLYQLFTQQALAKRQQSNPNAKRPLIAVVTASA
HHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
RDPFEAAHYYVSVFEQIGAEVIWLPLDAALQAGIATRQCEQLEMFRQQIQGNTDRARLYP
CCCHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHCCH
KQVELQKDFCASPIKLYQLLNQIDGLFLNGGDQSLTLKAWTTPDESPSEALNIIKKRLSK
HHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCC
QEIVIGGTSAGTAVMADKNMITGGTSEGALSFGIFATEAPSERCEVANCDASVPATAVTY
CEEEEECCCCCCEEEECCCEECCCCCCCCEEEEEEEECCCHHHCCCCCCCCCCCCEEEEE
REQGGLGFFPFGIPDTHFSQRDRYLRLLLLSANADSKMAFGVDENTALLVDLSHRSLSVV
ECCCCCEEECCCCCCCCHHHHHHEEEEEEEECCCCCCEEECCCCCCEEEEEECCCCEEEE
GEHGVWALAQVERLKQSQANSEINAISHYLTPGSYAEIDATGEHLTHIRLAQTAKLTPIM
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHCCHHH
SKQSDFFTWVSNACRQGIYSTSLSDKYELTISPMAALSCQGILNQEHGYQSMGLTLKALE
CCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCHHHCCCHHHHC
Q
C
>Mature Secondary Structure
MEICSSQAPANCQSEVKFSADAKRETRYVFNVSWLERALKHPRLQSLNEQQKVILRQAAK
CCCCCCCCCCCCHHCCEECCCCCCCCEEEEEHHHHHHHHCCCHHHCCCHHHHHHHHHHHH
ALSPVSNSLENSDVEIGRSLGRLHSDSLTLAKLEQAIAKIDKQFLAHLSDQQYYALLDLL
HHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EEKQMLADGTLLQEQVLLDSTTNPHAATLYQLFTQQALAKRQQSNPNAKRPLIAVVTASA
HHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
RDPFEAAHYYVSVFEQIGAEVIWLPLDAALQAGIATRQCEQLEMFRQQIQGNTDRARLYP
CCCHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHCCH
KQVELQKDFCASPIKLYQLLNQIDGLFLNGGDQSLTLKAWTTPDESPSEALNIIKKRLSK
HHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCC
QEIVIGGTSAGTAVMADKNMITGGTSEGALSFGIFATEAPSERCEVANCDASVPATAVTY
CEEEEECCCCCCEEEECCCEECCCCCCCCEEEEEEEECCCHHHCCCCCCCCCCCCEEEEE
REQGGLGFFPFGIPDTHFSQRDRYLRLLLLSANADSKMAFGVDENTALLVDLSHRSLSVV
ECCCCCEEECCCCCCCCHHHHHHEEEEEEEECCCCCCEEECCCCCCEEEEEECCCCEEEE
GEHGVWALAQVERLKQSQANSEINAISHYLTPGSYAEIDATGEHLTHIRLAQTAKLTPIM
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHCCHHH
SKQSDFFTWVSNACRQGIYSTSLSDKYELTISPMAALSCQGILNQEHGYQSMGLTLKALE
CCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCHHHCCCHHHHC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11986309 [H]