Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 126173884

GI number: 126173884

Start: 1929077

End: 1929964

Strand: Reverse

Name: gcvA [H]

Synonym: Sbal_1652

Alternate gene names: 126173884

Gene position: 1929964-1929077 (Counterclockwise)

Preceding gene: 126173889

Following gene: 126173878

Centisome position: 37.64

GC content: 48.42

Gene sequence:

>888_bases
ATGTTGACCGAACTGCCACCACTTAATTCCCTCCGTGCTTTTGAAGCGGCTGCACGCTTGCAGAGTATGACATTAGCCAG
CAAGGAGCTGCATGTCACCCATGGTGCGGTCAGTAAACAAATCCGAGTATTAGAGGATTATTTGGGTTTTGCGCTTTTTG
TGCGCTTACATAAAAAAGTCAGTTTAACCGATGAAGCCAAGCGCTATTTGCCCCATGTCCAAGCCGCGCTGCAAACCCTG
TCAAGTGCAACGAGTGACCTACGCCGCCAGCCTTCTAAGCCACAAACCTTAGCCATCAATGTGCTGCCAAGCTTAACCAT
TAATTGGCTCATCCCGAGAATGGAGCAGTTTAAGTCCCGCCATCCGCACCTTTATGTCGATTTGTCGATTGGTGACTTTG
CCGTGGACTTTAGCCAAGGCCGTTATGATATTGCGATTCGCAGCTGCACAGCTATTCCTAAGGGCGCGAATTATATCAAA
CTGATGGATGAGGATTTGTGTTTAGTTTGCGCGCCATCGTTAGCGCCAAAACTGCAAAATATCGAAGATATCAATCACGT
CACCTTACTGAAACACACTACAAGACCCGAACTGTGGGAATATTGGGCAGAAAAAGTGGGCTTAACACTCACCCAAGTAC
AAACCTTTGGCGTCGAGCATTTCTACATGCTCAGCCAAGCCGCCGCGAGTGGCATGGGGGCAGCGCTTATTCCGCGATTT
TTTATCGAAGAGCAATTGGCAAACGGCAGTTTAGTCATTCCCTTTGACGCCCCTTTTACCAGCCCCTATCGCTATTACCT
GCTCACGCCCAAATCCCCTAGCCTGCCGTTAAAAGCCCAAGCTTTTATTGACTGGTTATTAGAAGTGTTTGCGCCTTATC
GCCACTAA

Upstream 100 bases:

>100_bases
GTTTGCCAAATAAACGCAAAGGAGTTATTGTCGTCATTGCGTGAGTTAAACTCACACAAATCTGTAATCCTGTTCTAATT
CCGCAACTGTAGGACATCAA

Downstream 100 bases:

>100_bases
ACAACATTCGGAATTTCACAGTTTCAGCAAGCTGCGATAACGCTATATGTGCTAAATAAAGCTATTGAGTGCTGCAAACA
GGCCAGAAAAACGGCAATAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MLTELPPLNSLRAFEAAARLQSMTLASKELHVTHGAVSKQIRVLEDYLGFALFVRLHKKVSLTDEAKRYLPHVQAALQTL
SSATSDLRRQPSKPQTLAINVLPSLTINWLIPRMEQFKSRHPHLYVDLSIGDFAVDFSQGRYDIAIRSCTAIPKGANYIK
LMDEDLCLVCAPSLAPKLQNIEDINHVTLLKHTTRPELWEYWAEKVGLTLTQVQTFGVEHFYMLSQAAASGMGAALIPRF
FIEEQLANGSLVIPFDAPFTSPYRYYLLTPKSPSLPLKAQAFIDWLLEVFAPYRH

Sequences:

>Translated_295_residues
MLTELPPLNSLRAFEAAARLQSMTLASKELHVTHGAVSKQIRVLEDYLGFALFVRLHKKVSLTDEAKRYLPHVQAALQTL
SSATSDLRRQPSKPQTLAINVLPSLTINWLIPRMEQFKSRHPHLYVDLSIGDFAVDFSQGRYDIAIRSCTAIPKGANYIK
LMDEDLCLVCAPSLAPKLQNIEDINHVTLLKHTTRPELWEYWAEKVGLTLTQVQTFGVEHFYMLSQAAASGMGAALIPRF
FIEEQLANGSLVIPFDAPFTSPYRYYLLTPKSPSLPLKAQAFIDWLLEVFAPYRH
>Mature_295_residues
MLTELPPLNSLRAFEAAARLQSMTLASKELHVTHGAVSKQIRVLEDYLGFALFVRLHKKVSLTDEAKRYLPHVQAALQTL
SSATSDLRRQPSKPQTLAINVLPSLTINWLIPRMEQFKSRHPHLYVDLSIGDFAVDFSQGRYDIAIRSCTAIPKGANYIK
LMDEDLCLVCAPSLAPKLQNIEDINHVTLLKHTTRPELWEYWAEKVGLTLTQVQTFGVEHFYMLSQAAASGMGAALIPRF
FIEEQLANGSLVIPFDAPFTSPYRYYLLTPKSPSLPLKAQAFIDWLLEVFAPYRH

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=35.7388316151203, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1788706, Length=291, Percent_Identity=31.9587628865979, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786448, Length=284, Percent_Identity=31.3380281690141, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=25.6055363321799, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693193, Length=302, Percent_Identity=25.8278145695364, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790262, Length=261, Percent_Identity=27.5862068965517, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787601, Length=172, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788887, Length=165, Percent_Identity=28.4848484848485, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33292; Mature: 33292

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTELPPLNSLRAFEAAARLQSMTLASKELHVTHGAVSKQIRVLEDYLGFALFVRLHKKV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SLTDEAKRYLPHVQAALQTLSSATSDLRRQPSKPQTLAINVLPSLTINWLIPRMEQFKSR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCC
HPHLYVDLSIGDFAVDFSQGRYDIAIRSCTAIPKGANYIKLMDEDLCLVCAPSLAPKLQN
CCEEEEEEECCCEEEECCCCEEEEEEEEEECCCCCCCEEEEECCCEEEEECCHHCHHHCC
IEDINHVTLLKHTTRPELWEYWAEKVGLTLTQVQTFGVEHFYMLSQAAASGMGAALIPRF
HHHCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHH
FIEEQLANGSLVIPFDAPFTSPYRYYLLTPKSPSLPLKAQAFIDWLLEVFAPYRH
HHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLTELPPLNSLRAFEAAARLQSMTLASKELHVTHGAVSKQIRVLEDYLGFALFVRLHKKV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SLTDEAKRYLPHVQAALQTLSSATSDLRRQPSKPQTLAINVLPSLTINWLIPRMEQFKSR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCC
HPHLYVDLSIGDFAVDFSQGRYDIAIRSCTAIPKGANYIKLMDEDLCLVCAPSLAPKLQN
CCEEEEEEECCCEEEECCCCEEEEEEEEEECCCCCCCEEEEECCCEEEEECCHHCHHHCC
IEDINHVTLLKHTTRPELWEYWAEKVGLTLTQVQTFGVEHFYMLSQAAASGMGAALIPRF
HHHCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHH
FIEEQLANGSLVIPFDAPFTSPYRYYLLTPKSPSLPLKAQAFIDWLLEVFAPYRH
HHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]