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The map label for this gene is pspF [H]

Identifier: 126173850

GI number: 126173850

Start: 1888441

End: 1889532

Strand: Reverse

Name: pspF [H]

Synonym: Sbal_1616

Alternate gene names: 126173850

Gene position: 1889532-1888441 (Counterclockwise)

Preceding gene: 126173858

Following gene: 126173849

Centisome position: 36.85

GC content: 48.81

Gene sequence:

>1092_bases
GTGGATAATAAATTTCAGCAAGACAACCTTATCGGTCAATCCAACGCCCTACTCGAAGTGTTAGAACATGTGTCGCAAAT
TGCGCCGCTGTCTAAACCCGTACTGATTATTGGTGAACGTGGCACAGGTAAAGAGCTTATTGCCGAACGCTTACATTATC
TGTCTAAGCGCTGGGATCAAAGTTTTATTAAGCTAAACTGTTCATCTTTGAGTGAAAATTTACTCGAGAGCGAATTATTT
GGCCATGAGTCAGGTGCCTTTACGGGCGCCAAAGGCAAACACGAAGGTCGCTTTGAGCGCGCCGACGGCGGCACTTTATT
CCTCGACGAATTAGCCAATACCTCAGGACTTATCCAAGAAAAGCTTCTGCGCGTCATTGAATACGGCGAATTTGAACGAG
TCGGCGGCAGTAAAACCGTGCAAAGTGATGTTCGTTTAATCTGCGCCGCCAACGAAGATCTGCCCTCACTTGCTGACGCA
GGCGAATTTCGTGCCGACTTGCTCGATAGACTCGCCTTCGATGTGATCACTCTGCCGCCGCTGCGTTGCCGCCCAGAAGA
CATAATGACGCTGGCGGAATACTTCGCCGTTGGCATGGCACGCCAATTAAAACTGCAACTGTTCAGCGGTTTTAGTGCCC
ATGCGGTCGAGCAATTAATGACCTACGACTGGCCGGGCAATATCCGTGAACTTAAAAACGTGGTCGAGCGCAGCGTTTAC
CGTAATGGTGGTGAAAATCGGCCCATCGACCATATCACCCTCGATCCCTTTGCTTCACCCTATCGACCTAAAACTCGGAT
CAAGACCCGCGAACGCCAAATCACCTCAGTCGCGCCTACAACCGTTGCCAGTACGCCGCAATCTGCGCCGATACTTGAGG
CTGAAGTACCCGCCAATACTGAGTCTTCAGTGAATAGCTTTAATTTTCCGCTGGATTTTAAAGAGCAAACTGAGAAGTAC
GAGATGGATCTGATCCAACAGGCGCTCTCAAGCAGCCAATACAACCAAAAGAAAACCGCCGACATGCTCGGATTAAGCTA
CCACCAACTGCGTGGCATCCTTAAAAAATACAATTTACTTGATAAAGCATAA

Upstream 100 bases:

>100_bases
ACAGCAATTTAAACAAAATCTTAACTTTTACTTCCTTTTACGCTTCAAGCCACTTATTATCATTTTCACCAAATTTTAGC
GCGAAAGACTAAAAAATATC

Downstream 100 bases:

>100_bases
GCAGATTGCCCATGGGGGAGATGCACTGCTAAAATAGCCGTTCATAGTCGATAAAATAATGATTTATAAATGAGTGTGCT
AATTAGACGCCTGTGCCTAA

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MDNKFQQDNLIGQSNALLEVLEHVSQIAPLSKPVLIIGERGTGKELIAERLHYLSKRWDQSFIKLNCSSLSENLLESELF
GHESGAFTGAKGKHEGRFERADGGTLFLDELANTSGLIQEKLLRVIEYGEFERVGGSKTVQSDVRLICAANEDLPSLADA
GEFRADLLDRLAFDVITLPPLRCRPEDIMTLAEYFAVGMARQLKLQLFSGFSAHAVEQLMTYDWPGNIRELKNVVERSVY
RNGGENRPIDHITLDPFASPYRPKTRIKTRERQITSVAPTTVASTPQSAPILEAEVPANTESSVNSFNFPLDFKEQTEKY
EMDLIQQALSSSQYNQKKTADMLGLSYHQLRGILKKYNLLDKA

Sequences:

>Translated_363_residues
MDNKFQQDNLIGQSNALLEVLEHVSQIAPLSKPVLIIGERGTGKELIAERLHYLSKRWDQSFIKLNCSSLSENLLESELF
GHESGAFTGAKGKHEGRFERADGGTLFLDELANTSGLIQEKLLRVIEYGEFERVGGSKTVQSDVRLICAANEDLPSLADA
GEFRADLLDRLAFDVITLPPLRCRPEDIMTLAEYFAVGMARQLKLQLFSGFSAHAVEQLMTYDWPGNIRELKNVVERSVY
RNGGENRPIDHITLDPFASPYRPKTRIKTRERQITSVAPTTVASTPQSAPILEAEVPANTESSVNSFNFPLDFKEQTEKY
EMDLIQQALSSSQYNQKKTADMLGLSYHQLRGILKKYNLLDKA
>Mature_363_residues
MDNKFQQDNLIGQSNALLEVLEHVSQIAPLSKPVLIIGERGTGKELIAERLHYLSKRWDQSFIKLNCSSLSENLLESELF
GHESGAFTGAKGKHEGRFERADGGTLFLDELANTSGLIQEKLLRVIEYGEFERVGGSKTVQSDVRLICAANEDLPSLADA
GEFRADLLDRLAFDVITLPPLRCRPEDIMTLAEYFAVGMARQLKLQLFSGFSAHAVEQLMTYDWPGNIRELKNVVERSVY
RNGGENRPIDHITLDPFASPYRPKTRIKTRERQITSVAPTTVASTPQSAPILEAEVPANTESSVNSFNFPLDFKEQTEKY
EMDLIQQALSSSQYNQKKTADMLGLSYHQLRGILKKYNLLDKA

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=353, Percent_Identity=57.2237960339943, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI87082117, Length=238, Percent_Identity=49.1596638655462, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1788550, Length=359, Percent_Identity=37.883008356546, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1790437, Length=239, Percent_Identity=48.1171548117155, Blast_Score=221, Evalue=5e-59,
Organism=Escherichia coli, GI1789087, Length=244, Percent_Identity=48.3606557377049, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI87082152, Length=364, Percent_Identity=35.1648351648352, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1789233, Length=357, Percent_Identity=35.8543417366947, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1790299, Length=353, Percent_Identity=36.5439093484419, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788905, Length=233, Percent_Identity=42.4892703862661, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1786524, Length=250, Percent_Identity=41.6, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1787583, Length=247, Percent_Identity=40.4858299595142, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1789828, Length=228, Percent_Identity=35.5263157894737, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87081858, Length=211, Percent_Identity=32.2274881516588, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 40726; Mature: 40726

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNKFQQDNLIGQSNALLEVLEHVSQIAPLSKPVLIIGERGTGKELIAERLHYLSKRWDQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCH
SFIKLNCSSLSENLLESELFGHESGAFTGAKGKHEGRFERADGGTLFLDELANTSGLIQE
HHHEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCEEEHHHHCCCHHHHHH
KLLRVIEYGEFERVGGSKTVQSDVRLICAANEDLPSLADAGEFRADLLDRLAFDVITLPP
HHHHHHHCCCHHHCCCCHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
LRCRPEDIMTLAEYFAVGMARQLKLQLFSGFSAHAVEQLMTYDWPGNIRELKNVVERSVY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RNGGENRPIDHITLDPFASPYRPKTRIKTRERQITSVAPTTVASTPQSAPILEAEVPANT
HCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCCEECCCCCCC
ESSVNSFNFPLDFKEQTEKYEMDLIQQALSSSQYNQKKTADMLGLSYHQLRGILKKYNLL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHH
DKA
CCC
>Mature Secondary Structure
MDNKFQQDNLIGQSNALLEVLEHVSQIAPLSKPVLIIGERGTGKELIAERLHYLSKRWDQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCH
SFIKLNCSSLSENLLESELFGHESGAFTGAKGKHEGRFERADGGTLFLDELANTSGLIQE
HHHEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCEEEHHHHCCCHHHHHH
KLLRVIEYGEFERVGGSKTVQSDVRLICAANEDLPSLADAGEFRADLLDRLAFDVITLPP
HHHHHHHCCCHHHCCCCHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
LRCRPEDIMTLAEYFAVGMARQLKLQLFSGFSAHAVEQLMTYDWPGNIRELKNVVERSVY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RNGGENRPIDHITLDPFASPYRPKTRIKTRERQITSVAPTTVASTPQSAPILEAEVPANT
HCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCCEECCCCCCC
ESSVNSFNFPLDFKEQTEKYEMDLIQQALSSSQYNQKKTADMLGLSYHQLRGILKKYNLL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHH
DKA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]