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The map label for this gene is folD

Identifier: 126173836

GI number: 126173836

Start: 1870897

End: 1871751

Strand: Reverse

Name: folD

Synonym: Sbal_1602

Alternate gene names: 126173836

Gene position: 1871751-1870897 (Counterclockwise)

Preceding gene: 126173843

Following gene: 126173834

Centisome position: 36.51

GC content: 49.36

Gene sequence:

>855_bases
ATGACAGCCCAAATAATCGACGGCAAGGCGATTGCTCAATCCATCCGCACTAAGCTCAGCGAAAAAGTCACCGCCCGCAA
AGAGGCTGGACAGCGCATTCCGGGTTTAGCCGTAGTATTAGTGGGAGCCGATCCGGCATCACAGGTTTATGTTGGAAGCA
AACGTAAGGCCTGTGAAGAAGTTGGATTTATTTCTCGTTCATACGATTTAGAAACAAGTTGCAGTGAAGATGAGTTGCTG
AGCCTTATTGATAGTCTGAATGATGATCCGACCATCGATGGCATTTTAGTGCAACTGCCACTACCCGCCCATATCGAAGA
TTCAAAAGTCATCGAACGTATTCGCCCAGATAAAGATGTCGATGGATTTCACCCGTACAACGTTGGCCGTTTAGCCCAGC
GTATTCCGGTGCTGCGCTCTTGCACGCCAATGGGCATAATGACACTCATCAAATCCACCGGCGTTGATACCTATGGTTTA
GATGCTGTCGTGGTTGGTGCGTCTAATATTGTCGGCCGCCCTATGACGTTAGAATTACTGTTGGCGGGTTGCACTACGAC
CACTTGCCACCGTTTCACTAAGAATCTTGAGCAAAAAATTCGTATCGCAGATCTCGTGGTTGTCGCAGTCGGTAAACCCG
GCTTCATTCCTGGTGAGTGGATCAAGCCCGGCGCTATCGTCATTGACGTAGGCATTAACCGTTTAGATAACGGCACCTTA
GTCGGCGATGTGCAATATGACGTTGCGGCGCAAAATGCCAGCTTTATCACTCCAGTCCCCGGCGGTGTTGGCCCGATGAC
GATTGCGAGTCTATTGGAAAACACCTTATACGCTGCCGAGCAATATCACGACTAA

Upstream 100 bases:

>100_bases
CATGACACAGTAGCTGTGCCCTGTTAAAATAGCCGCGATTGCCAATCTCTGTGTTAACTACTGTTTTAACCATCGCCCAT
ATAAAGGATATCCTACACCC

Downstream 100 bases:

>100_bases
GTTCTAAGTTCTAAGTTCTAAGTTCTAAGTTCTAAGTTCTAAGTTCTAAGTTCTAAGTTCTAAGTTCTGCAAGCATAAAA
AAACCTGCATGATGCAGGTT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTAQIIDGKAIAQSIRTKLSEKVTARKEAGQRIPGLAVVLVGADPASQVYVGSKRKACEEVGFISRSYDLETSCSEDELL
SLIDSLNDDPTIDGILVQLPLPAHIEDSKVIERIRPDKDVDGFHPYNVGRLAQRIPVLRSCTPMGIMTLIKSTGVDTYGL
DAVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKNLEQKIRIADLVVVAVGKPGFIPGEWIKPGAIVIDVGINRLDNGTL
VGDVQYDVAAQNASFITPVPGGVGPMTIASLLENTLYAAEQYHD

Sequences:

>Translated_284_residues
MTAQIIDGKAIAQSIRTKLSEKVTARKEAGQRIPGLAVVLVGADPASQVYVGSKRKACEEVGFISRSYDLETSCSEDELL
SLIDSLNDDPTIDGILVQLPLPAHIEDSKVIERIRPDKDVDGFHPYNVGRLAQRIPVLRSCTPMGIMTLIKSTGVDTYGL
DAVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKNLEQKIRIADLVVVAVGKPGFIPGEWIKPGAIVIDVGINRLDNGTL
VGDVQYDVAAQNASFITPVPGGVGPMTIASLLENTLYAAEQYHD
>Mature_283_residues
TAQIIDGKAIAQSIRTKLSEKVTARKEAGQRIPGLAVVLVGADPASQVYVGSKRKACEEVGFISRSYDLETSCSEDELLS
LIDSLNDDPTIDGILVQLPLPAHIEDSKVIERIRPDKDVDGFHPYNVGRLAQRIPVLRSCTPMGIMTLIKSTGVDTYGLD
AVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKNLEQKIRIADLVVVAVGKPGFIPGEWIKPGAIVIDVGINRLDNGTLV
GDVQYDVAAQNASFITPVPGGVGPMTIASLLENTLYAAEQYHD

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=298, Percent_Identity=46.3087248322148, Blast_Score=234, Evalue=6e-62,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=42.1768707482993, Blast_Score=214, Evalue=7e-56,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=43.1972789115646, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI36796743, Length=220, Percent_Identity=27.7272727272727, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786741, Length=284, Percent_Identity=71.4788732394366, Blast_Score=425, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17568735, Length=294, Percent_Identity=39.4557823129252, Blast_Score=184, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=40.9395973154362, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=42.1602787456446, Blast_Score=220, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6322933, Length=311, Percent_Identity=26.6881028938907, Blast_Score=82, Evalue=8e-17,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=47.8114478114478, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=47.8114478114478, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=44.8275862068966, Blast_Score=227, Evalue=6e-60,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=44.8275862068966, Blast_Score=227, Evalue=6e-60,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=44.8275862068966, Blast_Score=227, Evalue=8e-60,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=44.8275862068966, Blast_Score=227, Evalue=8e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_SHEB2 (B8E5F1)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002358663.1
- ProteinModelPortal:   B8E5F1
- SMR:   B8E5F1
- GeneID:   7087131
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_2752
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- ProtClustDB:   PRK10792
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30435; Mature: 30304

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQIIDGKAIAQSIRTKLSEKVTARKEAGQRIPGLAVVLVGADPASQVYVGSKRKACEE
CCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCHHHHHHH
VGFISRSYDLETSCSEDELLSLIDSLNDDPTIDGILVQLPLPAHIEDSKVIERIRPDKDV
HHCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCC
DGFHPYNVGRLAQRIPVLRSCTPMGIMTLIKSTGVDTYGLDAVVVGASNIVGRPMTLELL
CCCCCCCHHHHHHHCCHHHCCCCHHHHHHHHHCCCCCCCCCEEEEECHHHCCCCCEEEHH
LAGCTTTTCHRFTKNLEQKIRIADLVVVAVGKPGFIPGEWIKPGAIVIDVGINRLDNGTL
HHCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEE
VGDVQYDVAAQNASFITPVPGGVGPMTIASLLENTLYAAEQYHD
EEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAQIIDGKAIAQSIRTKLSEKVTARKEAGQRIPGLAVVLVGADPASQVYVGSKRKACEE
CCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCHHHHHHH
VGFISRSYDLETSCSEDELLSLIDSLNDDPTIDGILVQLPLPAHIEDSKVIERIRPDKDV
HHCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCC
DGFHPYNVGRLAQRIPVLRSCTPMGIMTLIKSTGVDTYGLDAVVVGASNIVGRPMTLELL
CCCCCCCHHHHHHHCCHHHCCCCHHHHHHHHHCCCCCCCCCEEEEECHHHCCCCCEEEHH
LAGCTTTTCHRFTKNLEQKIRIADLVVVAVGKPGFIPGEWIKPGAIVIDVGINRLDNGTL
HHCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEE
VGDVQYDVAAQNASFITPVPGGVGPMTIASLLENTLYAAEQYHD
EEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA