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The map label for this gene is insO2 [H]

Identifier: 126173585

GI number: 126173585

Start: 1544170

End: 1544982

Strand: Reverse

Name: insO2 [H]

Synonym: Sbal_1347

Alternate gene names: 126173585

Gene position: 1544982-1544170 (Counterclockwise)

Preceding gene: 126173586

Following gene: 126173584

Centisome position: 30.13

GC content: 42.19

Gene sequence:

>813_bases
GTGCATCGAAGTAGCTATAAGTATTGGCTAAAAAGAAAAGAACATATTGATGCAGACTTTACTATTTTATGCAGTGAAGT
GAAGGCTGCACATCGTATTAGTCATGGCTCTGCCGGTGCGCGAACGATAGCGCAATTAGTGACTAACAAAGGGATTGGTC
TCAGTCGTTATCGAGCCCGTAATCTAATGAAAAAGCTTGGGTTATTAAGCTGTCAGCAGCCTAAGCATTCTTATAGAAAA
GCGACACAAGAGCATGTTGCAATCCCCAATACGCTTAATCGACAGTTTGCAGTAACTCAACCAGATCAAGTGTGGTGTGG
CGACGTGACGTATGTTTGGGTCGGTAATCGTTGGGCCTATCTAGCCGTTGTTTTAGACTTATTTTCCCGCAAACCTGTTG
GATGGGCTTTGTCATTATCACCAGATAGTGAGCTGACCTGTAAAGCTTTGAAAATGGCATTTGAGTTAAGGGGGAAGCCT
GAGAATGTGATATATCACAGCGATCAAGGTTCGCATTACACAAGCTTGAAATTCAGACAATTAATATGGCGTCTTCAGAT
TGAGCAAAGTATGAGTCGTCGCGGGAATTGTTGGGATAACGCACCAATGGAGCGTTTCTTTAGAAGTTTGAAATCTGAGT
GGATACCCGCCAGTGGTTATGGGAATTTTACAGAAGCAGATAAAGAGATCACAAATTACATCACTGGATATTACAGTGAG
ACCAGACCCCATCAATATAATGGTGGGTTAACGCCAAATGAGTCAGAACGTTTATATTGGAATGACTCTAAAACCGTGGC
CAATTTTACTTGA

Upstream 100 bases:

>100_bases
AGGCTACCGCTCTCTTGATGTCAGACTCGATGAACAATTTACGCTAATCAGCGCACTCAAGCAGAGCCACTCTATTCTCA
CAATTTGTAATGCATTCAAA

Downstream 100 bases:

>100_bases
CCACTACAGTATGAGTTACGACGGGCGTGTCAGTGTTAAGTCTATCGGCTACGGTATGCACCTGTTTAAATACCATGGTT
GTATCATGTTGGCGACCCGT

Product: integrase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MHRSSYKYWLKRKEHIDADFTILCSEVKAAHRISHGSAGARTIAQLVTNKGIGLSRYRARNLMKKLGLLSCQQPKHSYRK
ATQEHVAIPNTLNRQFAVTQPDQVWCGDVTYVWVGNRWAYLAVVLDLFSRKPVGWALSLSPDSELTCKALKMAFELRGKP
ENVIYHSDQGSHYTSLKFRQLIWRLQIEQSMSRRGNCWDNAPMERFFRSLKSEWIPASGYGNFTEADKEITNYITGYYSE
TRPHQYNGGLTPNESERLYWNDSKTVANFT

Sequences:

>Translated_270_residues
MHRSSYKYWLKRKEHIDADFTILCSEVKAAHRISHGSAGARTIAQLVTNKGIGLSRYRARNLMKKLGLLSCQQPKHSYRK
ATQEHVAIPNTLNRQFAVTQPDQVWCGDVTYVWVGNRWAYLAVVLDLFSRKPVGWALSLSPDSELTCKALKMAFELRGKP
ENVIYHSDQGSHYTSLKFRQLIWRLQIEQSMSRRGNCWDNAPMERFFRSLKSEWIPASGYGNFTEADKEITNYITGYYSE
TRPHQYNGGLTPNESERLYWNDSKTVANFT
>Mature_270_residues
MHRSSYKYWLKRKEHIDADFTILCSEVKAAHRISHGSAGARTIAQLVTNKGIGLSRYRARNLMKKLGLLSCQQPKHSYRK
ATQEHVAIPNTLNRQFAVTQPDQVWCGDVTYVWVGNRWAYLAVVLDLFSRKPVGWALSLSPDSELTCKALKMAFELRGKP
ENVIYHSDQGSHYTSLKFRQLIWRLQIEQSMSRRGNCWDNAPMERFFRSLKSEWIPASGYGNFTEADKEITNYITGYYSE
TRPHQYNGGLTPNESERLYWNDSKTVANFT

Specific function: Involved in the transposition of the insertion sequence IS911B [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=191, Percent_Identity=31.413612565445, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1788405, Length=167, Percent_Identity=40.1197604790419, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787263, Length=167, Percent_Identity=40.1197604790419, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786753, Length=167, Percent_Identity=40.1197604790419, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786570, Length=167, Percent_Identity=40.1197604790419, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786490, Length=167, Percent_Identity=40.1197604790419, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789981, Length=256, Percent_Identity=33.984375, Blast_Score=124, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31210; Mature: 31210

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRSSYKYWLKRKEHIDADFTILCSEVKAAHRISHGSAGARTIAQLVTNKGIGLSRYRAR
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHH
NLMKKLGLLSCQQPKHSYRKATQEHVAIPNTLNRQFAVTQPDQVWCGDVTYVWVGNRWAY
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEECCEEEEEECCCHHH
LAVVLDLFSRKPVGWALSLSPDSELTCKALKMAFELRGKPENVIYHSDQGSHYTSLKFRQ
HHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCEEHHHHHHH
LIWRLQIEQSMSRRGNCWDNAPMERFFRSLKSEWIPASGYGNFTEADKEITNYITGYYSE
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
TRPHQYNGGLTPNESERLYWNDSKTVANFT
CCCCCCCCCCCCCCCCEEEECCCCEEECCC
>Mature Secondary Structure
MHRSSYKYWLKRKEHIDADFTILCSEVKAAHRISHGSAGARTIAQLVTNKGIGLSRYRAR
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHH
NLMKKLGLLSCQQPKHSYRKATQEHVAIPNTLNRQFAVTQPDQVWCGDVTYVWVGNRWAY
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEECCEEEEEECCCHHH
LAVVLDLFSRKPVGWALSLSPDSELTCKALKMAFELRGKPENVIYHSDQGSHYTSLKFRQ
HHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCEEHHHHHHH
LIWRLQIEQSMSRRGNCWDNAPMERFFRSLKSEWIPASGYGNFTEADKEITNYITGYYSE
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
TRPHQYNGGLTPNESERLYWNDSKTVANFT
CCCCCCCCCCCCCCCCEEEECCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]