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The map label for this gene is rotA [H]

Identifier: 126173567

GI number: 126173567

Start: 1514035

End: 1514664

Strand: Reverse

Name: rotA [H]

Synonym: Sbal_1329

Alternate gene names: 126173567

Gene position: 1514664-1514035 (Counterclockwise)

Preceding gene: 126173577

Following gene: 126173560

Centisome position: 29.54

GC content: 46.67

Gene sequence:

>630_bases
ATGAAGAAGTTATCAGGATTACTGTTTGTCGGCTGCATAGCCTTAACCGGTTGTGAAGGAAGTACTGATGGTGATCTAAC
GCCGACGCCACCGACGCCCGATCCCACTCCGCCAGCCTTAGCTGCGGATGTATGTTATTTAATGAAAACCACGAAAGGCG
ATATCACACTCGCCATTGACTTAACGAACATGCCCATCACAGGTAAAAACTTTAAGCAGTATGTTGATAAGTCTTTCTAT
AACGGCACCTTATTCCATAGAACCGTTAATAACTTTATGATCCAAGGCGGCGGTTTTACCACTGGACTCAAAAGCAAACC
AGGCGATGCCCCCATCAAGAATGAAGCCGCAGTCGGTATTAGCAACAAACGTGGCACTATCTCCATGGCTAGAACCACAG
TGCCAGATTCTGCAACATCACAGTTCTTTATCAATGTGCTAGATAATCCCGATCTGGATGCGTCCGCTAGCGAATTTGGT
TATGCCGTGTTTGGCAAAGTGGTTGACGGCATGGATGTGGCGGATCAGATCAGCATAGTACCAACAAAAAGTAGCGGTGG
TTTCGCCAACACGCCAGTACAAGAAATTCTGATTAACTCTGTGGCTACAACAAGCTGCCCTGCTGCTTAA

Upstream 100 bases:

>100_bases
TTCATGGAGCCTGCTATGCCCTCGCTTATCCGCCAAAGGCTCGCTCGATGGAACGCTAAAGCAGAAACTCCCCAATGATG
ATTGAGAATAAGGATATGAA

Downstream 100 bases:

>100_bases
GTATGTAAAAGATGCACAGCTAAACGACATCGAGTTAAAAGCAAAAAAGCACTGAATCTTCAGTGCTTTCTCATTTTAAA
AACGAATCGATTTTAGCGTC

Product: peptidyl-prolyl cis-trans isomerase cyclophilin type

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MKKLSGLLFVGCIALTGCEGSTDGDLTPTPPTPDPTPPALAADVCYLMKTTKGDITLAIDLTNMPITGKNFKQYVDKSFY
NGTLFHRTVNNFMIQGGGFTTGLKSKPGDAPIKNEAAVGISNKRGTISMARTTVPDSATSQFFINVLDNPDLDASASEFG
YAVFGKVVDGMDVADQISIVPTKSSGGFANTPVQEILINSVATTSCPAA

Sequences:

>Translated_209_residues
MKKLSGLLFVGCIALTGCEGSTDGDLTPTPPTPDPTPPALAADVCYLMKTTKGDITLAIDLTNMPITGKNFKQYVDKSFY
NGTLFHRTVNNFMIQGGGFTTGLKSKPGDAPIKNEAAVGISNKRGTISMARTTVPDSATSQFFINVLDNPDLDASASEFG
YAVFGKVVDGMDVADQISIVPTKSSGGFANTPVQEILINSVATTSCPAA
>Mature_209_residues
MKKLSGLLFVGCIALTGCEGSTDGDLTPTPPTPDPTPPALAADVCYLMKTTKGDITLAIDLTNMPITGKNFKQYVDKSFY
NGTLFHRTVNNFMIQGGGFTTGLKSKPGDAPIKNEAAVGISNKRGTISMARTTVPDSATSQFFINVLDNPDLDASASEFG
YAVFGKVVDGMDVADQISIVPTKSSGGFANTPVQEILINSVATTSCPAA

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. This protein is not essential for growth. Presumably plays a role in signal transduction [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI19557636, Length=165, Percent_Identity=30.9090909090909, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI22547212, Length=196, Percent_Identity=31.6326530612245, Blast_Score=73, Evalue=1e-13,
Organism=Homo sapiens, GI7657473, Length=196, Percent_Identity=31.6326530612245, Blast_Score=73, Evalue=1e-13,
Organism=Homo sapiens, GI22547215, Length=196, Percent_Identity=32.1428571428571, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI7706339, Length=155, Percent_Identity=30.9677419354839, Blast_Score=68, Evalue=6e-12,
Organism=Escherichia coli, GI1789763, Length=167, Percent_Identity=53.8922155688623, Blast_Score=173, Evalue=8e-45,
Organism=Escherichia coli, GI1786736, Length=161, Percent_Identity=49.0683229813665, Blast_Score=153, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71980594, Length=166, Percent_Identity=31.9277108433735, Blast_Score=77, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71980590, Length=137, Percent_Identity=35.036496350365, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17539496, Length=156, Percent_Identity=33.974358974359, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71997782, Length=126, Percent_Identity=37.3015873015873, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17552780, Length=153, Percent_Identity=33.9869281045752, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320510, Length=154, Percent_Identity=37.6623376623377, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321848, Length=124, Percent_Identity=38.7096774193548, Blast_Score=63, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17986117, Length=139, Percent_Identity=33.0935251798561, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922912, Length=158, Percent_Identity=32.9113924050633, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 21852; Mature: 21852

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLSGLLFVGCIALTGCEGSTDGDLTPTPPTPDPTPPALAADVCYLMKTTKGDITLAID
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
LTNMPITGKNFKQYVDKSFYNGTLFHRTVNNFMIQGGGFTTGLKSKPGDAPIKNEAAVGI
ECCCCCCCCHHHHHHHHHHCCCCHHEEECCCEEEECCCEECCCCCCCCCCCCCCCEEEEE
SNKRGTISMARTTVPDSATSQFFINVLDNPDLDASASEFGYAVFGKVVDGMDVADQISIV
CCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
PTKSSGGFANTPVQEILINSVATTSCPAA
EECCCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKLSGLLFVGCIALTGCEGSTDGDLTPTPPTPDPTPPALAADVCYLMKTTKGDITLAID
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
LTNMPITGKNFKQYVDKSFYNGTLFHRTVNNFMIQGGGFTTGLKSKPGDAPIKNEAAVGI
ECCCCCCCCHHHHHHHHHHCCCCHHEEECCCEEEECCCEECCCCCCCCCCCCCCCEEEEE
SNKRGTISMARTTVPDSATSQFFINVLDNPDLDASASEFGYAVFGKVVDGMDVADQISIV
CCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
PTKSSGGFANTPVQEILINSVATTSCPAA
EECCCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7948017 [H]