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The map label for this gene is spoU [C]

Identifier: 125624162

GI number: 125624162

Start: 1317707

End: 1318297

Strand: Direct

Name: spoU [C]

Synonym: llmg_1343

Alternate gene names: 125624162

Gene position: 1317707-1318297 (Clockwise)

Preceding gene: 125624144

Following gene: 125624163

Centisome position: 52.09

GC content: 35.03

Gene sequence:

>591_bases
TTGTTTTTTACTTTAATTAATTTACTGACGGGTTCTGACATAACCGTCAGTAAAATTTTTGTTATTAAGAAAGTGTATCA
CATGAATCATATTGCTCTTTTTGAACCTCGTATCCATTTCAATACAGGAAATATTGCTCGTACCTGTGCAGCTACAAATA
CTGTTTTACATTTGATTGAACCTTTTGGTTTTGAAATTTCAGATAAACACCTCAAACGTGCTGGTCTGGACTATTGGGAT
AAAGTAAATATTGTCTATCATAAAAATTTACAAGACTTTATGAACTCAATTGCTGACGGAGGCCAACTTTATTTGGTCAG
TAAATTTGCAGAACACGTTTATTCTGATGTTGACTATTCTGATGATAAAAAAGACCACTACTTCTTGTTTGGCCGTGAAG
ATACTGGACTACCTGAAGAATTTATGCACGAACACCGTGACGAATGTATCCGTATCCCAATGAATGACGAACACGTCCGC
TCATTAAATCTTTCTAACTGCGCATGCATGATCGTCTACGAAGCACTCAAACAACAAGAATTCCGTGGATTAGAACTTGT
CCATACTTATGAAAAAGACAAGTTAAAATAA

Upstream 100 bases:

>100_bases
AATTATATTAATTTGTTTCAATCTTTCCTGTTATGATTAAAGCAAATTAGTCCCCCAACTAGACTTCTTGCTGAGATTGA
CTCCGAGGGGTCTAGTTTTT

Downstream 100 bases:

>100_bases
TTTTAACTATATATTTATTTACAAAAAGTTTTGCAAATTTGCAGAGCTTTTTTCTATTGTATGTTAAAGGCCGAAGTGCT
ATAATGGTGCCAAACGAAGA

Product: putative rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MFFTLINLLTGSDITVSKIFVIKKVYHMNHIALFEPRIHFNTGNIARTCAATNTVLHLIEPFGFEISDKHLKRAGLDYWD
KVNIVYHKNLQDFMNSIADGGQLYLVSKFAEHVYSDVDYSDDKKDHYFLFGREDTGLPEEFMHEHRDECIRIPMNDEHVR
SLNLSNCACMIVYEALKQQEFRGLELVHTYEKDKLK

Sequences:

>Translated_196_residues
MFFTLINLLTGSDITVSKIFVIKKVYHMNHIALFEPRIHFNTGNIARTCAATNTVLHLIEPFGFEISDKHLKRAGLDYWD
KVNIVYHKNLQDFMNSIADGGQLYLVSKFAEHVYSDVDYSDDKKDHYFLFGREDTGLPEEFMHEHRDECIRIPMNDEHVR
SLNLSNCACMIVYEALKQQEFRGLELVHTYEKDKLK
>Mature_196_residues
MFFTLINLLTGSDITVSKIFVIKKVYHMNHIALFEPRIHFNTGNIARTCAATNTVLHLIEPFGFEISDKHLKRAGLDYWD
KVNIVYHKNLQDFMNSIADGGQLYLVSKFAEHVYSDVDYSDDKKDHYFLFGREDTGLPEEFMHEHRDECIRIPMNDEHVR
SLNLSNCACMIVYEALKQQEFRGLELVHTYEKDKLK

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=160, Percent_Identity=39.375, Blast_Score=109, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 22936; Mature: 22936

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFTLINLLTGSDITVSKIFVIKKVYHMNHIALFEPRIHFNTGNIARTCAATNTVLHLIE
CCEEEEEHHCCCCCHHHHHHHHHHHHHCCEEEEECCEEEECCCCHHHHHHHHHHHHHHHH
PFGFEISDKHLKRAGLDYWDKVNIVYHKNLQDFMNSIADGGQLYLVSKFAEHVYSDVDYS
CCCCCCCHHHHHHCCCCCHHCEEEEEECCHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCC
DDKKDHYFLFGREDTGLPEEFMHEHRDECIRIPMNDEHVRSLNLSNCACMIVYEALKQQE
CCCCCEEEEEECCCCCCCHHHHHHHHHHEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHH
FRGLELVHTYEKDKLK
CCCCCEEEECCCCCCC
>Mature Secondary Structure
MFFTLINLLTGSDITVSKIFVIKKVYHMNHIALFEPRIHFNTGNIARTCAATNTVLHLIE
CCEEEEEHHCCCCCHHHHHHHHHHHHHCCEEEEECCEEEECCCCHHHHHHHHHHHHHHHH
PFGFEISDKHLKRAGLDYWDKVNIVYHKNLQDFMNSIADGGQLYLVSKFAEHVYSDVDYS
CCCCCCCHHHHHHCCCCCHHCEEEEEECCHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCC
DDKKDHYFLFGREDTGLPEEFMHEHRDECIRIPMNDEHVRSLNLSNCACMIVYEALKQQE
CCCCCEEEEEECCCCCCCHHHHHHHHHHEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHH
FRGLELVHTYEKDKLK
CCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA