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The map label for this gene is thiD [H]

Identifier: 124485667

GI number: 124485667

Start: 828147

End: 828947

Strand: Reverse

Name: thiD [H]

Synonym: Mlab_0845

Alternate gene names: 124485667

Gene position: 828947-828147 (Counterclockwise)

Preceding gene: 124485669

Following gene: 124485666

Centisome position: 45.93

GC content: 59.8

Gene sequence:

>801_bases
ATGGCCTTCGCCGCCACGATCGCAGGCTCCGACCCCTCCGCCGGCGCAGGGATCCAGGTCGACCTGAAAACCATGGCAGC
CTGCGGCGTCTGGGGGATGACCGTGATCGCGGCCCTGACCGCCCAGAACGCCACGCACGTTCTCGATTCATCGAGCGTTC
CCGCCGCCTTCATCAAAAAGCAGATCGACGCCCTCGAAGAGGATTTCCCTATCGGCTGCTACAAAACGGGCATGCTCAAA
AATGCGGAGACGGTGAATATCGTTGCCGCCTCCATTCCCGAAGGGCGAAACCTCGTCGTCGACCCGGTGCTTCTCGCGAC
AAAAGAATACCGGCTTCTCGACGAGGCGGGCCAGGAGCGGCTCGTTGCCGAACTTCTGCCGCGGACCACCGTGATAACTC
CGAACATCCCCGAAGCCGCGGCCCTTTCGGGAATCACCATCACCGGGTCTGAGTCGATGGAAGAGGCGGCATACTGGTTC
ATAGATCACGGGGCAAAGGCCGCGGTGATCAAAGGCGGCCACGCACATTTCCGGCTCGGGACCGACGTCTTTGCCGACAA
AGACGGCATCATGCTGGTCGAAGGGGAAGTCGCGCCTTTCACGGACGTTCACGGATCGGGTTGCTGCTATGCGTCCGCGA
TCGCGGCCCACATCGCTCTCGGCTATCCGGTCAGGGATGCCGTATTAGAGGCAAAAAAGTTCGTTACCGGCGCAATAAAA
TACTCATGGGAATATGCTCCGGGCCGCAGGACCATGAATCCCGGCTGGCAGCAGCACAAAACCTATTATACAAAATTCTG
A

Upstream 100 bases:

>100_bases
TGAAGGGACAGACCATAAAGACATGAGGTTAAACTCCCGAAGAACTATTTTTTCAAAGGAGCACACTAATATATCTGATA
TGAATTCCCGCGAAAATACG

Downstream 100 bases:

>100_bases
CATTCTTTTTTCCGTCGTGAAAAAACCCACCTCTATCTTTATGATAAAATAAAGCAAACTATAACCCCATGACCGAAACG
GCACGCAAAGCCGGGCGCAG

Product: hypothetical protein

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MAFAATIAGSDPSAGAGIQVDLKTMAACGVWGMTVIAALTAQNATHVLDSSSVPAAFIKKQIDALEEDFPIGCYKTGMLK
NAETVNIVAASIPEGRNLVVDPVLLATKEYRLLDEAGQERLVAELLPRTTVITPNIPEAAALSGITITGSESMEEAAYWF
IDHGAKAAVIKGGHAHFRLGTDVFADKDGIMLVEGEVAPFTDVHGSGCCYASAIAAHIALGYPVRDAVLEAKKFVTGAIK
YSWEYAPGRRTMNPGWQQHKTYYTKF

Sequences:

>Translated_266_residues
MAFAATIAGSDPSAGAGIQVDLKTMAACGVWGMTVIAALTAQNATHVLDSSSVPAAFIKKQIDALEEDFPIGCYKTGMLK
NAETVNIVAASIPEGRNLVVDPVLLATKEYRLLDEAGQERLVAELLPRTTVITPNIPEAAALSGITITGSESMEEAAYWF
IDHGAKAAVIKGGHAHFRLGTDVFADKDGIMLVEGEVAPFTDVHGSGCCYASAIAAHIALGYPVRDAVLEAKKFVTGAIK
YSWEYAPGRRTMNPGWQQHKTYYTKF
>Mature_265_residues
AFAATIAGSDPSAGAGIQVDLKTMAACGVWGMTVIAALTAQNATHVLDSSSVPAAFIKKQIDALEEDFPIGCYKTGMLKN
AETVNIVAASIPEGRNLVVDPVLLATKEYRLLDEAGQERLVAELLPRTTVITPNIPEAAALSGITITGSESMEEAAYWFI
DHGAKAAVIKGGHAHFRLGTDVFADKDGIMLVEGEVAPFTDVHGSGCCYASAIAAHIALGYPVRDAVLEAKKFVTGAIKY
SWEYAPGRRTMNPGWQQHKTYYTKF

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=239, Percent_Identity=35.9832635983264, Blast_Score=128, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6325378, Length=247, Percent_Identity=30.3643724696356, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324997, Length=247, Percent_Identity=32.3886639676113, Blast_Score=89, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324517, Length=247, Percent_Identity=31.5789473684211, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28321; Mature: 28189

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFAATIAGSDPSAGAGIQVDLKTMAACGVWGMTVIAALTAQNATHVLDSSSVPAAFIKK
CCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHH
QIDALEEDFPIGCYKTGMLKNAETVNIVAASIPEGRNLVVDPVLLATKEYRLLDEAGQER
HHHHHHCCCCCCEEECCCCCCCCEEEEEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHH
LVAELLPRTTVITPNIPEAAALSGITITGSESMEEAAYWFIDHGAKAAVIKGGHAHFRLG
HHHHHCCCCEEECCCCCHHHHHCCEEEECCCCHHHHEEEEEECCCEEEEEECCCEEEEEC
TDVFADKDGIMLVEGEVAPFTDVHGSGCCYASAIAAHIALGYPVRDAVLEAKKFVTGAIK
CHHEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEE
YSWEYAPGRRTMNPGWQQHKTYYTKF
EEEEECCCCCCCCCCHHHHCEEECCC
>Mature Secondary Structure 
AFAATIAGSDPSAGAGIQVDLKTMAACGVWGMTVIAALTAQNATHVLDSSSVPAAFIKK
CEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHH
QIDALEEDFPIGCYKTGMLKNAETVNIVAASIPEGRNLVVDPVLLATKEYRLLDEAGQER
HHHHHHCCCCCCEEECCCCCCCCEEEEEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHH
LVAELLPRTTVITPNIPEAAALSGITITGSESMEEAAYWFIDHGAKAAVIKGGHAHFRLG
HHHHHCCCCEEECCCCCHHHHHCCEEEECCCCHHHHEEEEEECCCEEEEEECCCEEEEEC
TDVFADKDGIMLVEGEVAPFTDVHGSGCCYASAIAAHIALGYPVRDAVLEAKKFVTGAIK
CHHEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEE
YSWEYAPGRRTMNPGWQQHKTYYTKF
EEEEECCCCCCCCCCHHHHCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]