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The map label for this gene is truD [H]

Identifier: 124485625

GI number: 124485625

Start: 783354

End: 784622

Strand: Reverse

Name: truD [H]

Synonym: Mlab_0803

Alternate gene names: 124485625

Gene position: 784622-783354 (Counterclockwise)

Preceding gene: 124485626

Following gene: 124485624

Centisome position: 43.47

GC content: 57.92

Gene sequence:

>1269_bases
ATGAAACCATCCACCCACCCCCTCGAAATCGACCTCGGCATGCGGTACTACGCCACCAGCCAGCCCGGAATCGGCGGACG
TCTCCGAACCACCCCCGAAGACTTCGTCGTCGAAGAACTGCCCATCACCTTCACCAACACCGGCCCCTACACCATCTGCA
AACTCACCAAACGCTCCTGGGAACACCAGCACGCCATGCACGAAATCACCAACCGGCTGCGCATCAGCCAGAAACGCATC
GGATGGGCAGGCACCAAAGACAAAAACGCCGTCACCACCCAGTACATCTCCCTCTACAACGTCCCCGCCGAAGCCGCCGC
CAACCTCAACATCAAAGACATGACCATCGAACCGGTCGCGACCCACCAGTTCTCCCTCGGCCTTGGAAACCTCCTTGGAA
ACAGATTCAAGATCACCCTTCGGGACTGCGAACCGAACGATCTCGCAAAAAACACCGCTGAAATCTCCGGCGAAATCGCT
GCAGGAATCCCCAACTACTACGGACTCCAGAGATTCGGCGCCCTCAAACCCGTCACCCACAAAATGGGTTACCACATCCT
CAGAAAAGAATTCAAAGAAGCCGTCGATCTCTACGTCGGAGGCTGCTTCCCCTACGAATCCGAACAGGTCCAAACCGCCC
GCAAAAACTTCGCCGAGACCGGCGACGCAAAAACCGCCCTCTACGAACTGCCGCCCTGGCTCTCGTATGAGCGGATCATG
CTCGACTCCCTCGCCAAAAACCCCGGCGACTACGGAGCGGCCCTTCAGGCAATGCCCCCAAAACTCCTCTCCATGTTCGT
CTCGGCATACCAGTCCTGGCTCTTCAACATCGCCCTTTCCAAACGGTGCGAAGAAAACGCCCCCCTCAACGAGCCGAGAG
TCGGCGAACACCTCGAATTCACCAACGGACGCGTCGACACCGTCACCGAGAAAAACATCGCGACCGCCCGCCAGCACATG
AAACGCGGCCGATGCTTCATCGTCGGCTGGATGCCCGGAAAGACCCTGCCCGTGGCCCCCGGTCCTCTCGAAGAAACGAT
GTTTGCCCAGATGGAAAAGGACAACATCTCCATGCAGAGCTTTGCCGACGCAACAGAGTTTGTAAAAACCAACTTCGACG
GCGCCCACCGAAGGATCTCCCTCGCAACCGAAGTAGAAACCGCGGTGTTTGAAAACAACGTGCAGCTGAACTTCGTTCTG
CCGCCCGGCCATTATGCGACCACCGTCGCACGGGAGTTCATGCAGGCCGCTCCCGAAAAAATGGTCTGA

Upstream 100 bases:

>100_bases
TACACCGAAATCCCCCCGGGAACGGTCACGGCGCTTGGAATAGGACCCGCAAAAGCCGAAATCATCAATAAAATAACCGG
ACACCTCCCCCTCCTCTGAC

Downstream 100 bases:

>100_bases
CGGCCAATTCCTTTTTTGAAACAGTCGGATTTCGAAAACCCGGATTCGCGATACATATAAGGTGCCTGCAGGCAACTCTA
CACCTATGAGGAAACGTGCA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKPSTHPLEIDLGMRYYATSQPGIGGRLRTTPEDFVVEELPITFTNTGPYTICKLTKRSWEHQHAMHEITNRLRISQKRI
GWAGTKDKNAVTTQYISLYNVPAEAAANLNIKDMTIEPVATHQFSLGLGNLLGNRFKITLRDCEPNDLAKNTAEISGEIA
AGIPNYYGLQRFGALKPVTHKMGYHILRKEFKEAVDLYVGGCFPYESEQVQTARKNFAETGDAKTALYELPPWLSYERIM
LDSLAKNPGDYGAALQAMPPKLLSMFVSAYQSWLFNIALSKRCEENAPLNEPRVGEHLEFTNGRVDTVTEKNIATARQHM
KRGRCFIVGWMPGKTLPVAPGPLEETMFAQMEKDNISMQSFADATEFVKTNFDGAHRRISLATEVETAVFENNVQLNFVL
PPGHYATTVAREFMQAAPEKMV

Sequences:

>Translated_422_residues
MKPSTHPLEIDLGMRYYATSQPGIGGRLRTTPEDFVVEELPITFTNTGPYTICKLTKRSWEHQHAMHEITNRLRISQKRI
GWAGTKDKNAVTTQYISLYNVPAEAAANLNIKDMTIEPVATHQFSLGLGNLLGNRFKITLRDCEPNDLAKNTAEISGEIA
AGIPNYYGLQRFGALKPVTHKMGYHILRKEFKEAVDLYVGGCFPYESEQVQTARKNFAETGDAKTALYELPPWLSYERIM
LDSLAKNPGDYGAALQAMPPKLLSMFVSAYQSWLFNIALSKRCEENAPLNEPRVGEHLEFTNGRVDTVTEKNIATARQHM
KRGRCFIVGWMPGKTLPVAPGPLEETMFAQMEKDNISMQSFADATEFVKTNFDGAHRRISLATEVETAVFENNVQLNFVL
PPGHYATTVAREFMQAAPEKMV
>Mature_422_residues
MKPSTHPLEIDLGMRYYATSQPGIGGRLRTTPEDFVVEELPITFTNTGPYTICKLTKRSWEHQHAMHEITNRLRISQKRI
GWAGTKDKNAVTTQYISLYNVPAEAAANLNIKDMTIEPVATHQFSLGLGNLLGNRFKITLRDCEPNDLAKNTAEISGEIA
AGIPNYYGLQRFGALKPVTHKMGYHILRKEFKEAVDLYVGGCFPYESEQVQTARKNFAETGDAKTALYELPPWLSYERIM
LDSLAKNPGDYGAALQAMPPKLLSMFVSAYQSWLFNIALSKRCEENAPLNEPRVGEHLEFTNGRVDTVTEKNIATARQHM
KRGRCFIVGWMPGKTLPVAPGPLEETMFAQMEKDNISMQSFADATEFVKTNFDGAHRRISLATEVETAVFENNVQLNFVL
PPGHYATTVAREFMQAAPEKMV

Specific function: Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Homo sapiens, GI50727002, Length=406, Percent_Identity=24.8768472906404, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI148762985, Length=250, Percent_Identity=31.2, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI148762961, Length=250, Percent_Identity=31.2, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI148762948, Length=250, Percent_Identity=31.2, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1789102, Length=162, Percent_Identity=30.8641975308642, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17557232, Length=322, Percent_Identity=27.639751552795, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324817, Length=249, Percent_Identity=32.9317269076305, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21355799, Length=225, Percent_Identity=31.1111111111111, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 47346; Mature: 47346

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS50984 TRUD ; PS01268 UPF0024

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSTHPLEIDLGMRYYATSQPGIGGRLRTTPEDFVVEELPITFTNTGPYTICKLTKRSW
CCCCCCCEEEECCCEEEECCCCCCCCCCCCCCHHHHHCCCCEEEECCCCEEEEHHHHHHH
EHQHAMHEITNRLRISQKRIGWAGTKDKNAVTTQYISLYNVPAEAAANLNIKDMTIEPVA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEEHHEECCCHHHHCCCCEEEEEECCCC
THQFSLGLGNLLGNRFKITLRDCEPNDLAKNTAEISGEIAAGIPNYYGLQRFGALKPVTH
CCCHHCCHHHHCCCEEEEEEECCCCCHHHHHHHHHCCHHCCCCCCHHHHHHHCCCCHHHH
KMGYHILRKEFKEAVDLYVGGCFPYESEQVQTARKNFAETGDAKTALYELPPWLSYERIM
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
LDSLAKNPGDYGAALQAMPPKLLSMFVSAYQSWLFNIALSKRCEENAPLNEPRVGEHLEF
HHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEC
TNGRVDTVTEKNIATARQHMKRGRCFIVGWMPGKTLPVAPGPLEETMFAQMEKDNISMQS
CCCEEEEECHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHH
FADATEFVKTNFDGAHRRISLATEVETAVFENNVQLNFVLPPGHYATTVAREFMQAAPEK
HHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCC
MV
CC
>Mature Secondary Structure
MKPSTHPLEIDLGMRYYATSQPGIGGRLRTTPEDFVVEELPITFTNTGPYTICKLTKRSW
CCCCCCCEEEECCCEEEECCCCCCCCCCCCCCHHHHHCCCCEEEECCCCEEEEHHHHHHH
EHQHAMHEITNRLRISQKRIGWAGTKDKNAVTTQYISLYNVPAEAAANLNIKDMTIEPVA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEEHHEECCCHHHHCCCCEEEEEECCCC
THQFSLGLGNLLGNRFKITLRDCEPNDLAKNTAEISGEIAAGIPNYYGLQRFGALKPVTH
CCCHHCCHHHHCCCEEEEEEECCCCCHHHHHHHHHCCHHCCCCCCHHHHHHHCCCCHHHH
KMGYHILRKEFKEAVDLYVGGCFPYESEQVQTARKNFAETGDAKTALYELPPWLSYERIM
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
LDSLAKNPGDYGAALQAMPPKLLSMFVSAYQSWLFNIALSKRCEENAPLNEPRVGEHLEF
HHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEC
TNGRVDTVTEKNIATARQHMKRGRCFIVGWMPGKTLPVAPGPLEETMFAQMEKDNISMQS
CCCEEEEECHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHH
FADATEFVKTNFDGAHRRISLATEVETAVFENNVQLNFVLPPGHYATTVAREFMQAAPEK
HHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCC
MV
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA