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The map label for this gene is ddl

Identifier: 124265657

GI number: 124265657

Start: 504922

End: 505881

Strand: Direct

Name: ddl

Synonym: Mpe_A0464

Alternate gene names: 124265657

Gene position: 504922-505881 (Clockwise)

Preceding gene: 124265656

Following gene: 124265658

Centisome position: 12.49

GC content: 70.31

Gene sequence:

>960_bases
ATGAGCGCCACCCCCGATCCCCGGTCCTTCGGCAAGGTCGCCGTGCTGATGGGCGGCACCTCGGCCGAACGCGACATCTC
GCTGATGTCCGGCGGCGGCGTGCTGAAGGCGCTGCAGGACGCCGGCGTCGACGCCCACGCCTTCGATCCGAAGGATCACG
AACTGGTCGAGCTGCGGCGCCAGGGCTTCCAGCGCTGCTTCATTGCGCTGCACGGTCGGCACGGCGAGGACGGCACGGTG
CAGGGCGCGCTGGAGCTGCTGCGCATTCCGTACACCGGTTCGGGCGTGATGGCGAGCGCCATCGCGATGGACAAGATCAT
GACCAAGCGCGTGTGGCTCGCCGAAGGGCTGCCGACGCCACGCTACGTGCGGCTCGCGCCCGACGAGCAGACGCCCGAGC
GGGTGCGCGGCGTGCCCGACGACCTGGGCCTGCCGTTGATCGTGAAGCCGCCGCGCGAGGGCTCGTCGATCGGCGTCACC
AAGGTGCTCGGCTATTCGCAGATGCAGGACGCGGTGGCGCTGTCGGCCCGCCACGATCCCGACGTGCTGTGCGAGGAGTT
CATCGACGGCGCCGAGGTGACCTGCCCGGTGCTGGGCGAGGGCGCGAATGCGCGCGCGCTGCCGGTGATCCGCATCGTGC
CGCCCGAGGCCGGCTACGACTACCAGAACAAGTACTTCACCGACGAGGTGAAGTACCTCTGTCCGAGCGGCCTGCCGGCG
GACGAGGAGGCCGAGATCCAGCGCATCGTGCTGGCCGCCTACCGAGCGCTGGGCTGCCGCGGCTGGGGCCGGGCCGACCT
GATGATCCGCGCCAGCGATCGCAAGCCCTTCCTGCTGGAGATGAACACCTCGCCGGGCATGACCGGCCATTCGCTGGTGC
CGATGTCGGCCAAGGCCGCAGGCCTCGGCTACGAGCAGCTGTGCCTGCACATCCTGCAGGGAGCGGCGCTCGACGCCTGA

Upstream 100 bases:

>100_bases
TCGCCGACAACGTGCAGGACGGCGATGTGGTGATCACCATGGGGGCGGGCTCGATCGGCGCCGTGCCCGGCCAGGTGGTC
GAACTGTTGGGAGCCCACGC

Downstream 100 bases:

>100_bases
GCCCCTCATGGCCCGCAGACCCCGCCTCCCCGCGAAGCCGCAGCCGCTGCCGGCCGATGTGCGCCTGATGCGCACCGGCG
CCAGCGTGCTGTTCGCGTTC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSATPDPRSFGKVAVLMGGTSAERDISLMSGGGVLKALQDAGVDAHAFDPKDHELVELRRQGFQRCFIALHGRHGEDGTV
QGALELLRIPYTGSGVMASAIAMDKIMTKRVWLAEGLPTPRYVRLAPDEQTPERVRGVPDDLGLPLIVKPPREGSSIGVT
KVLGYSQMQDAVALSARHDPDVLCEEFIDGAEVTCPVLGEGANARALPVIRIVPPEAGYDYQNKYFTDEVKYLCPSGLPA
DEEAEIQRIVLAAYRALGCRGWGRADLMIRASDRKPFLLEMNTSPGMTGHSLVPMSAKAAGLGYEQLCLHILQGAALDA

Sequences:

>Translated_319_residues
MSATPDPRSFGKVAVLMGGTSAERDISLMSGGGVLKALQDAGVDAHAFDPKDHELVELRRQGFQRCFIALHGRHGEDGTV
QGALELLRIPYTGSGVMASAIAMDKIMTKRVWLAEGLPTPRYVRLAPDEQTPERVRGVPDDLGLPLIVKPPREGSSIGVT
KVLGYSQMQDAVALSARHDPDVLCEEFIDGAEVTCPVLGEGANARALPVIRIVPPEAGYDYQNKYFTDEVKYLCPSGLPA
DEEAEIQRIVLAAYRALGCRGWGRADLMIRASDRKPFLLEMNTSPGMTGHSLVPMSAKAAGLGYEQLCLHILQGAALDA
>Mature_318_residues
SATPDPRSFGKVAVLMGGTSAERDISLMSGGGVLKALQDAGVDAHAFDPKDHELVELRRQGFQRCFIALHGRHGEDGTVQ
GALELLRIPYTGSGVMASAIAMDKIMTKRVWLAEGLPTPRYVRLAPDEQTPERVRGVPDDLGLPLIVKPPREGSSIGVTK
VLGYSQMQDAVALSARHDPDVLCEEFIDGAEVTCPVLGEGANARALPVIRIVPPEAGYDYQNKYFTDEVKYLCPSGLPAD
EEAEIQRIVLAAYRALGCRGWGRADLMIRASDRKPFLLEMNTSPGMTGHSLVPMSAKAAGLGYEQLCLHILQGAALDA

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=309, Percent_Identity=51.4563106796116, Blast_Score=320, Evalue=7e-89,
Organism=Escherichia coli, GI1786579, Length=348, Percent_Identity=31.0344827586207, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_METPP (A2SCY7)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001019661.1
- ProteinModelPortal:   A2SCY7
- SMR:   A2SCY7
- STRING:   A2SCY7
- GeneID:   4784183
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0464
- NMPDR:   fig|279263.3.peg.2708
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   SWIVEDA
- PhylomeDB:   A2SCY7
- ProtClustDB:   PRK01372
- BioCyc:   MPET420662:MPE_A0464-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 34334; Mature: 34203

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATPDPRSFGKVAVLMGGTSAERDISLMSGGGVLKALQDAGVDAHAFDPKDHELVELRR
CCCCCCCCCCCCEEEEECCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCHHHHHHHH
QGFQRCFIALHGRHGEDGTVQGALELLRIPYTGSGVMASAIAMDKIMTKRVWLAEGLPTP
HHHHEEEHHEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
RYVRLAPDEQTPERVRGVPDDLGLPLIVKPPREGSSIGVTKVLGYSQMQDAVALSARHDP
CEEEECCCCCCHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHCHHHHHHHHHHHCCCCH
DVLCEEFIDGAEVTCPVLGEGANARALPVIRIVPPEAGYDYQNKYFTDEVKYLCPSGLPA
HHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCEECCHHHHHCCCCCCC
DEEAEIQRIVLAAYRALGCRGWGRADLMIRASDRKPFLLEMNTSPGMTGHSLVPMSAKAA
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCEECCCCCHHH
GLGYEQLCLHILQGAALDA
CCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SATPDPRSFGKVAVLMGGTSAERDISLMSGGGVLKALQDAGVDAHAFDPKDHELVELRR
CCCCCCCCCCCEEEEECCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCHHHHHHHH
QGFQRCFIALHGRHGEDGTVQGALELLRIPYTGSGVMASAIAMDKIMTKRVWLAEGLPTP
HHHHEEEHHEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
RYVRLAPDEQTPERVRGVPDDLGLPLIVKPPREGSSIGVTKVLGYSQMQDAVALSARHDP
CEEEECCCCCCHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHCHHHHHHHHHHHCCCCH
DVLCEEFIDGAEVTCPVLGEGANARALPVIRIVPPEAGYDYQNKYFTDEVKYLCPSGLPA
HHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCEECCHHHHHCCCCCCC
DEEAEIQRIVLAAYRALGCRGWGRADLMIRASDRKPFLLEMNTSPGMTGHSLVPMSAKAA
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCEECCCCCHHH
GLGYEQLCLHILQGAALDA
CCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA