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The map label for this gene is murF [H]

Identifier: 124265651

GI number: 124265651

Start: 496552

End: 497964

Strand: Direct

Name: murF [H]

Synonym: Mpe_A0458

Alternate gene names: 124265651

Gene position: 496552-497964 (Clockwise)

Preceding gene: 124265650

Following gene: 124265652

Centisome position: 12.28

GC content: 74.31

Gene sequence:

>1413_bases
ATGAGCGTGATGATGGACTTGCGGTTGGCGCATGCGCTGCTGCCGGGCTCGACGCTCGTCGGCGACCCGGCCACGCCGCT
GTTGCGCGTGCACAGCGATACCCGCACGCTGCGCGCCGGTGACCTGTTCGTTGCGCTCAAGGGCGAGCGCTTCGACGCGC
ACGACTTCCTGCCGCAGGCGCGTGCCGCCGGCGCAGTGGCGGCGATCGCCGAGCACGGCCTCGCCGAGGCCGGCCTGGCC
GGGCTGCACGTGGCCGACAGCCGCGCCGCGCTCGGCACGCTGGGCGCGAACTGGCGCGCCCGCATGAGCCTGCCGCTGAT
CGCTGTGACCGGCAGCAACGGCAAGACCACCGTCACGCAGATGATCGCGAGCATCCTGCGCGCCTGGGTCGGCGACGCGG
CATTCGCGACCGAGGGCAACTTCAACAACGACATCGGCGTGCCGCTCACGCTGCTGCGGTTGCGCCAGGACGCCGACACC
TGGCACCGCGCGGCGGTCGTCGAGCTGGGCATGAACCACGTCGGCGAGATCGCCCAGCTCGCGCGCATGGCCGCGCCGAC
GGTCGCGCTGGTCAACAACGCCCAGCGCGAGCACCAGGAATTCATGGCCAGCGTCGAGGCGGTGGCCCGCGAGAACGGCG
CGGTGATCGAGGTGCTGGGTGCCAACGGCACGGCGGTCTTCCCGTCCGACGACGCGCATGCCGCGGTATGGCAGGCGCTG
GCCGGCACGCGGCCGACGTTGACGTTCTCGCTCGAAGGTCCGTCCGACTTGGTGGCCGAGGCGGTGTGGAGCGGCGACCA
TTGGGCGGCGGTGCTGCACACGCCGGCCGGCGCCACCGCGATCCGCCTGCGCGTGGCCGGCTGGCACAACGTGAAGAACG
CGCTCGCCGCGACCGCCTGCGCCCTGGCGGCGGGCTGCCCGCTGGAGGCGGTGGCGCGTGGCCTCGAGGCCTTCGAGCCG
GTGAAGGGCCGCTCGCAGGTCAAGGCCCTGCAGCGCGGCGGCCGCGCGGTGACGCTGGTGGACGACAGCTACAACGCCAA
CCCCGACTCGGTGCGCGCGGCGATCGACGTGCTGGCCACGCTGCCCGGCCCGCGCTGGCTGGTGCTGGGCGACATGGGCG
AGGTCGGCGACCAGGGCCCGGCCTTCCACGCCGAGGTCGGCCGCTATGCGGCCGCTTGCGGCATCGACGCGCTGTGGACC
GCCGGCGCCGCCAGCGCCGCCGCCGCCGCCGCCTTCGGCCCGGCCGCACGGGCCCACGCGAGCGTGGCCGAGCTGATCGC
GGCGCTGGGGCCGGACACGCACGCCGCCCCGGAGGCCGCGTCCATCGTCGTCAAGGGCTCGCGCTTCATGAAGATGGAGC
AGGTGATCGCCGCGCTGCAGGCCGCTGGCGCCGCATCGCCCACCCCGCACTGA

Upstream 100 bases:

>100_bases
GCAAGGGCCACGAGGACTACCAGGAGATCGCCGGCGTCAAGCAGCCGTTCTCCGATGTGGCCGAGGCGCGGCAGGCGCTG
GCGGCGCGCGGGTTGGCGTC

Downstream 100 bases:

>100_bases
ACCGAAGGCACCGCAACTTCATGCTGATCTCCTTGTCGCAATGGCTGCAACTGCTCTACCCCGAGCAGCTCGGTTTCCTG
CGCGTGTTTCAGTACCTCAC

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSVMMDLRLAHALLPGSTLVGDPATPLLRVHSDTRTLRAGDLFVALKGERFDAHDFLPQARAAGAVAAIAEHGLAEAGLA
GLHVADSRAALGTLGANWRARMSLPLIAVTGSNGKTTVTQMIASILRAWVGDAAFATEGNFNNDIGVPLTLLRLRQDADT
WHRAAVVELGMNHVGEIAQLARMAAPTVALVNNAQREHQEFMASVEAVARENGAVIEVLGANGTAVFPSDDAHAAVWQAL
AGTRPTLTFSLEGPSDLVAEAVWSGDHWAAVLHTPAGATAIRLRVAGWHNVKNALAATACALAAGCPLEAVARGLEAFEP
VKGRSQVKALQRGGRAVTLVDDSYNANPDSVRAAIDVLATLPGPRWLVLGDMGEVGDQGPAFHAEVGRYAAACGIDALWT
AGAASAAAAAAFGPAARAHASVAELIAALGPDTHAAPEAASIVVKGSRFMKMEQVIAALQAAGAASPTPH

Sequences:

>Translated_470_residues
MSVMMDLRLAHALLPGSTLVGDPATPLLRVHSDTRTLRAGDLFVALKGERFDAHDFLPQARAAGAVAAIAEHGLAEAGLA
GLHVADSRAALGTLGANWRARMSLPLIAVTGSNGKTTVTQMIASILRAWVGDAAFATEGNFNNDIGVPLTLLRLRQDADT
WHRAAVVELGMNHVGEIAQLARMAAPTVALVNNAQREHQEFMASVEAVARENGAVIEVLGANGTAVFPSDDAHAAVWQAL
AGTRPTLTFSLEGPSDLVAEAVWSGDHWAAVLHTPAGATAIRLRVAGWHNVKNALAATACALAAGCPLEAVARGLEAFEP
VKGRSQVKALQRGGRAVTLVDDSYNANPDSVRAAIDVLATLPGPRWLVLGDMGEVGDQGPAFHAEVGRYAAACGIDALWT
AGAASAAAAAAFGPAARAHASVAELIAALGPDTHAAPEAASIVVKGSRFMKMEQVIAALQAAGAASPTPH
>Mature_469_residues
SVMMDLRLAHALLPGSTLVGDPATPLLRVHSDTRTLRAGDLFVALKGERFDAHDFLPQARAAGAVAAIAEHGLAEAGLAG
LHVADSRAALGTLGANWRARMSLPLIAVTGSNGKTTVTQMIASILRAWVGDAAFATEGNFNNDIGVPLTLLRLRQDADTW
HRAAVVELGMNHVGEIAQLARMAAPTVALVNNAQREHQEFMASVEAVARENGAVIEVLGANGTAVFPSDDAHAAVWQALA
GTRPTLTFSLEGPSDLVAEAVWSGDHWAAVLHTPAGATAIRLRVAGWHNVKNALAATACALAAGCPLEAVARGLEAFEPV
KGRSQVKALQRGGRAVTLVDDSYNANPDSVRAAIDVLATLPGPRWLVLGDMGEVGDQGPAFHAEVGRYAAACGIDALWTA
GAASAAAAAAFGPAARAHASVAELIAALGPDTHAAPEAASIVVKGSRFMKMEQVIAALQAAGAASPTPH

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=438, Percent_Identity=42.0091324200913, Blast_Score=248, Evalue=9e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48375; Mature: 48244

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVMMDLRLAHALLPGSTLVGDPATPLLRVHSDTRTLRAGDLFVALKGERFDAHDFLPQA
CCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEECCEEEEEECCCCCHHHCCHHH
RAAGAVAAIAEHGLAEAGLAGLHVADSRAALGTLGANWRARMSLPLIAVTGSNGKTTVTQ
HHHHHHHHHHHCCHHHCCCCCEEECCCCHHHHHCCCCCEEEECCEEEEEECCCCCHHHHH
MIASILRAWVGDAAFATEGNFNNDIGVPLTLLRLRQDADTWHRAAVVELGMNHVGEIAQL
HHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ARMAAPTVALVNNAQREHQEFMASVEAVARENGAVIEVLGANGTAVFPSDDAHAAVWQAL
HHHHCCHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCHHHHHHHHH
AGTRPTLTFSLEGPSDLVAEAVWSGDHWAAVLHTPAGATAIRLRVAGWHNVKNALAATAC
CCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHH
ALAAGCPLEAVARGLEAFEPVKGRSQVKALQRGGRAVTLVDDSYNANPDSVRAAIDVLAT
HHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH
LPGPRWLVLGDMGEVGDQGPAFHAEVGRYAAACGIDALWTAGAASAAAAAAFGPAARAHA
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHCCCHHHHHH
SVAELIAALGPDTHAAPEAASIVVKGSRFMKMEQVIAALQAAGAASPTPH
HHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SVMMDLRLAHALLPGSTLVGDPATPLLRVHSDTRTLRAGDLFVALKGERFDAHDFLPQA
CHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEECCEEEEEECCCCCHHHCCHHH
RAAGAVAAIAEHGLAEAGLAGLHVADSRAALGTLGANWRARMSLPLIAVTGSNGKTTVTQ
HHHHHHHHHHHCCHHHCCCCCEEECCCCHHHHHCCCCCEEEECCEEEEEECCCCCHHHHH
MIASILRAWVGDAAFATEGNFNNDIGVPLTLLRLRQDADTWHRAAVVELGMNHVGEIAQL
HHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ARMAAPTVALVNNAQREHQEFMASVEAVARENGAVIEVLGANGTAVFPSDDAHAAVWQAL
HHHHCCHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCHHHHHHHHH
AGTRPTLTFSLEGPSDLVAEAVWSGDHWAAVLHTPAGATAIRLRVAGWHNVKNALAATAC
CCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHH
ALAAGCPLEAVARGLEAFEPVKGRSQVKALQRGGRAVTLVDDSYNANPDSVRAAIDVLAT
HHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH
LPGPRWLVLGDMGEVGDQGPAFHAEVGRYAAACGIDALWTAGAASAAAAAAFGPAARAHA
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHCCCHHHHHH
SVAELIAALGPDTHAAPEAASIVVKGSRFMKMEQVIAALQAAGAASPTPH
HHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]