Click here to switch to the map view.

The map label for this gene is fmt

Identifier: 124265477

GI number: 124265477

Start: 309426

End: 310373

Strand: Direct

Name: fmt

Synonym: Mpe_A0284

Alternate gene names: 124265477

Gene position: 309426-310373 (Clockwise)

Preceding gene: 124265476

Following gene: 124265478

Centisome position: 7.65

GC content: 71.1

Gene sequence:

>948_bases
GTGCGCATCGCATTTGCCGGCACGCCCGAGTTCGCGCGTGTTGCGCTGAGGCAGTTGCTGGACGCCGGGTTCGACGTTCC
GCTGGTGCTGACGCAGCCCGATCGACCGGCGGGGCGCGGGCTCAAGTTGCAGGCCTCGGCCGTCAAGGCGCTGGCCGTGG
AGCGCGGCCTGGCCGTGACGCAGCCGCGCAGCCTGCGGCTGGACGGCAAGTACCCGGATGACGCAAGCGCGGCGCGCGAA
GCGCTCGAGGCGGCGCAGCTCGATGCGATGGTGGTGGCTGCCTATGGCCTGATCCTGCCGGCCTGGGTGCTGAAGCTGCC
GGCGCGTGGGTGCCTGAACATCCATGCCTCCTTGCTGCCGCGCTGGCGCGGGGCAGCACCGATCCACCGGGCGATCGAGG
CCGGCGATACGGAGACCGGTATCACCATCATGCAGATGGACGAAGGCCTGGATACCGGTGACATGCTGCTCTCCGAGCGC
GAGTCCATCCGATCCGACGACAGCACGGCGACGCTCCACGACCGCCTGTCGGCGCTCGGCGGTCGGCTGATCGTAGAAGC
GCTCGAGGCCGCCGCCTGCGGAGGACTGGTGCGGCGTCCGCAACCGGCGGAGGGCGTGACCTATGCCCACAAGATCGACA
AGGCGGAGGCGGCGATCGACTGGTCGCGGCCGGCCGAGGAGATCGAGCGCCGTGTGCGCGCCTTCGATCCCTTTCCCGGG
GCCAGTTTCCAGCAGGCCGGCGAGACAGTGAAGCTGTGGCGAGCCGCGGTGGCCCCGCAGCGAGGCGCGCCCGGCACGGT
GCTGAGCGCTGCCGAGGGCGTGCTGCGGGTTGCCTGCGGTGACCTGTCGCTGGACCTGCTGCAGCTGCAGCGTCCCGGCG
GCCGGCGCGTCGGGGCGCGGGATTTCCTGGCGGTTCGGTCGACACTGCAGGTCGGTGCAACACTGTGA

Upstream 100 bases:

>100_bases
AAGGTCTTCGTCGAGTACCTGTCCCCGCTCAAGCGCAACCGCATCAAGAGCAAGATGCTCAAGAAAACGCGCGACGAGGC
GCGAGACTGAGGCGGACTGC

Downstream 100 bases:

>100_bases
TGGTGCGGTGCGGGGATTGAACTCCCCGAGTTGTCCCGCATCTATGCCTGGGCGCCAGCGTGGCGCAGGAGCTGGAAACA
TGTTCAATCTGATGAAGACC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRIAFAGTPEFARVALRQLLDAGFDVPLVLTQPDRPAGRGLKLQASAVKALAVERGLAVTQPRSLRLDGKYPDDASAARE
ALEAAQLDAMVVAAYGLILPAWVLKLPARGCLNIHASLLPRWRGAAPIHRAIEAGDTETGITIMQMDEGLDTGDMLLSER
ESIRSDDSTATLHDRLSALGGRLIVEALEAAACGGLVRRPQPAEGVTYAHKIDKAEAAIDWSRPAEEIERRVRAFDPFPG
ASFQQAGETVKLWRAAVAPQRGAPGTVLSAAEGVLRVACGDLSLDLLQLQRPGGRRVGARDFLAVRSTLQVGATL

Sequences:

>Translated_315_residues
MRIAFAGTPEFARVALRQLLDAGFDVPLVLTQPDRPAGRGLKLQASAVKALAVERGLAVTQPRSLRLDGKYPDDASAARE
ALEAAQLDAMVVAAYGLILPAWVLKLPARGCLNIHASLLPRWRGAAPIHRAIEAGDTETGITIMQMDEGLDTGDMLLSER
ESIRSDDSTATLHDRLSALGGRLIVEALEAAACGGLVRRPQPAEGVTYAHKIDKAEAAIDWSRPAEEIERRVRAFDPFPG
ASFQQAGETVKLWRAAVAPQRGAPGTVLSAAEGVLRVACGDLSLDLLQLQRPGGRRVGARDFLAVRSTLQVGATL
>Mature_315_residues
MRIAFAGTPEFARVALRQLLDAGFDVPLVLTQPDRPAGRGLKLQASAVKALAVERGLAVTQPRSLRLDGKYPDDASAARE
ALEAAQLDAMVVAAYGLILPAWVLKLPARGCLNIHASLLPRWRGAAPIHRAIEAGDTETGITIMQMDEGLDTGDMLLSER
ESIRSDDSTATLHDRLSALGGRLIVEALEAAACGGLVRRPQPAEGVTYAHKIDKAEAAIDWSRPAEEIERRVRAFDPFPG
ASFQQAGETVKLWRAAVAPQRGAPGTVLSAAEGVLRVACGDLSLDLLQLQRPGGRRVGARDFLAVRSTLQVGATL

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=317, Percent_Identity=30.2839116719243, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI238814322, Length=326, Percent_Identity=28.2208588957055, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI164663775, Length=244, Percent_Identity=35.655737704918, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1789683, Length=308, Percent_Identity=49.6753246753247, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI1788589, Length=295, Percent_Identity=29.1525423728814, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI133930964, Length=272, Percent_Identity=31.25, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319458, Length=186, Percent_Identity=29.5698924731183, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45550868, Length=308, Percent_Identity=30.5194805194805, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28571984, Length=240, Percent_Identity=32.9166666666667, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24585660, Length=244, Percent_Identity=29.0983606557377, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_METPP (A2SCF7)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001019481.1
- ProteinModelPortal:   A2SCF7
- SMR:   A2SCF7
- STRING:   A2SCF7
- GeneID:   4786893
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0284
- NMPDR:   fig|279263.3.peg.170
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- PhylomeDB:   A2SCF7
- BioCyc:   MPET420662:MPE_A0284-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33584; Mature: 33584

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAFAGTPEFARVALRQLLDAGFDVPLVLTQPDRPAGRGLKLQASAVKALAVERGLAVT
CEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEHHHHHHHHHHHCCCEEC
QPRSLRLDGKYPDDASAAREALEAAQLDAMVVAAYGLILPAWVLKLPARGCLNIHASLLP
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
RWRGAAPIHRAIEAGDTETGITIMQMDEGLDTGDMLLSERESIRSDDSTATLHDRLSALG
CCCCCCHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GRLIVEALEAAACGGLVRRPQPAEGVTYAHKIDKAEAAIDWSRPAEEIERRVRAFDPFPG
HHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC
ASFQQAGETVKLWRAAVAPQRGAPGTVLSAAEGVLRVACGDLSLDLLQLQRPGGRRVGAR
CCHHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHH
DFLAVRSTLQVGATL
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MRIAFAGTPEFARVALRQLLDAGFDVPLVLTQPDRPAGRGLKLQASAVKALAVERGLAVT
CEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEHHHHHHHHHHHCCCEEC
QPRSLRLDGKYPDDASAAREALEAAQLDAMVVAAYGLILPAWVLKLPARGCLNIHASLLP
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
RWRGAAPIHRAIEAGDTETGITIMQMDEGLDTGDMLLSERESIRSDDSTATLHDRLSALG
CCCCCCHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GRLIVEALEAAACGGLVRRPQPAEGVTYAHKIDKAEAAIDWSRPAEEIERRVRAFDPFPG
HHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC
ASFQQAGETVKLWRAAVAPQRGAPGTVLSAAEGVLRVACGDLSLDLLQLQRPGGRRVGAR
CCHHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHH
DFLAVRSTLQVGATL
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA