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The map label for this gene is lptD [H]

Identifier: 124265395

GI number: 124265395

Start: 216425

End: 218884

Strand: Direct

Name: lptD [H]

Synonym: Mpe_A0202

Alternate gene names: 124265395

Gene position: 216425-218884 (Clockwise)

Preceding gene: 124265391

Following gene: 124265396

Centisome position: 5.35

GC content: 69.84

Gene sequence:

>2460_bases
TTGTCTGCCACGATCTTGCCGACCGCCGACCGCGCCGCGACCGTCCTGCCTCCACGGCGCGCCTGGGCCCTGCGCCCGGT
CGCGGCGGCGGTCCTGATGACGACCGCAGGTGGCGGCGTGCTGATCGACGTCGCACGCGCGCAGTCAGGAGCCGAAGGCC
CTGGACTGCAGGTGAGTCCCGAAAGCGGTGCGCGCTTCGGTCCACTCATCAAGGCCGACGACGGCGGCGGGCCGCTGGTG
ATCGAGGCTGATCGCCTGTCGGGCCGGCCTGACCTGGAAACTCTGGCCGAAGGTGCCGTCGAGTTCCGCCGCGGCCCCTT
GCTCCTGAAGGCTGACCGGGTCGAGTACCAGCAGCAGAGCGACTTGGCGCGCGCCACCGGCAACGTCGAGATCTCGCGCA
ACGGCAACATCTACCGTGGCCCCGAGGTGCAGCTGAAGGTCGACCGTTTCGAAGGCTACTTCCAGTCGCCGAGCTACCAC
TTCGGCCGCACCAACGCTGGTGGGCAGGCCGACCGCTTCGACTTCCTCGACGAGAACCGGGGCATCGCCTACGGCGCCAC
CTACACCAGCTGTCCGGCGCCCGACCCGGCCTGGCTGCTGAGCACCGACCGCGTGCTGCTCGACACGGCGGCCGAGGAAG
GCACGGCCGAGGGCGCGGTGCTGCGGTTCTATGGCGTGCCGATCCTGGCCGCGCCGACGCTGAGCTTCCCGCTGACCGAG
AAACGCAAGTCTGGCTGGTTGCCGCCGAGCATCAAGCTCGACAACAAGAGCGGCTTCGAGCTCGGCGTGCCCTACTACTG
GGACATCGCCCCGAACCGCGACGCGACCCTGACGCCGTCGGTCATGACGCGGCGCGGCGCGGCGCTCGACACCGAGTTCC
GCTATCTCGAGCCGGGCTACCTGGGCCGCGTTCAGGCCTATGCGCTGCCCGACGACCAGGTCGCCAATCGCTCGCGCTGG
GCCCTGAACCTCGGCCATGAGGCACGCTGGGAAGGCGCGACGGTCGGTGAGATCGACTACGGCCTGGCGCTGCAGCGCAC
CTCGGACGACAACTTCTGGAAGGACTTCGCCGGCACGCTGCCCAGCCTCACGCCGCGCCTGCTGCCGACCGATGCCTATG
CGCGGCGCCGCTTCGATCACGGCTGGGGCGACACCGTGGCCTACACCCGCGTGCAGACCTGGCAGGTGCTGCAGGATATC
GACCCGGCCAGCCGCATCGTCTCGCCCTACCAGCGCGAGCCGCAGATCGGCCTGCGCCAGCGCGGCGCATCCGGCGGGCT
CGAATGGCAGTGGGAGACCGAGGCGAACCGCTTCTCGAACGACGACCCCAGCCTGCAGACCGGCTCGCGCCTGCACGCCC
TGGGCTCGCTGGCACGCCCCTGGTCGCCGACCGGTGCGCCCGGCTGGACGCTGACACCCCGGGTGTCGTTCAACGCGGCC
AGCTACGACCTCGACCAGTCCCTGGCCGATGGCCGTCGGCGCGCGTCGCGTGTCATTCCGACGGTGTCGCTCGACAGCGC
CTGGATCTTCGAACGCGACAGCACGGCCTTCGGGCGCGAACTGACGCAGACGCTGGAGCCGCGGCTGTTCTATGTCAACA
CGCCCTACCGCGACCAGACCGGCCTGCCAAACTTCGACAGCGCGGCCAACGACTTCAACTTCACGTCGATCTACTCCGAC
AACGTGTTCTCCGGCGTCGATCGCGTATCGGACGCGAACCAGGTGACTGCCGGCGTGACCACGCGCTTTCTCGACCGCAC
GACCGGCGCCGAGGCGCTGCGGCTGGGCATCGCGCAGCGCATGCTGCTGCGCACGCAGCGCATCACGGCCGACGGCACGC
CGATCACGCAGCGCTGGTCCGACCTGCTGCTGCTGGGTTCGACCAACCTCGTGCGGAACTGGTACTTCGACAGCACCGTC
CAGTACAACTCCGACACCAGCCGCGTCGCGCGTTCCATTTCGAGCGTGCGCTACTCGCCCGGACCCTTGCGCACCCTCAG
CACCAGCTACCGCTACACGCGCAATGCCAGCGAACAGGTCGAGCTGGGCTGGCAATGGCCGCTCAACGCGCCGGCACGCG
AGCACGCCGCCGCCGTGCAGGCGCGCGACGCGCAGATGCTCGAGGACTTCGAGGGCCGCTCGCGGCTCGCGCCGCTGGCC
GGCTGCCCGGGCGCCTGGTACACGGTGGGGCGCGTCAACTACAGCCGCCGCGACTCCCGCATCACCGATTCCATCGTCGG
CTTCGAGTACGACTCGGGGTGCTGGATCGGCCGCATCGTCGCCGAGCGCCTGTCCACCGGGCGCAGCCAGGCGACCACCC
GGCTGCTGTTCCAGCTGGAGCTGATCGGCCTGTCGCGGCTCGGGTCCAACCCGTTGAAGGTGCTGAGGGAGAATGTGCCC
GGTTACCGTTTGCTGCGCGACGACAGTCCGGTGGTCGCGCCGGCCCTCTCGACCCCCTGA

Upstream 100 bases:

>100_bases
CGCGTGGAATAATCAGATTCTACAAACCGCATGCAAGGTGGCGTCGCCGTGGCGCTGCTCGGGCGGCCGCAGCGCGCCGC
GACCGGCGACTCCTTTTCTC

Downstream 100 bases:

>100_bases
GCTGCTGTCATCGATGTTGCCGAAGTCTTCTCCCCCTTGCGCCAGAGGGCTGCTGCTCGCGCTGCTCTGCAGCCTCTTCG
TCGCGAACGCGGTCGCGCAG

Product: putative organic solvent tolerance transmembrane protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 819; Mature: 818

Protein sequence:

>819_residues
MSATILPTADRAATVLPPRRAWALRPVAAAVLMTTAGGGVLIDVARAQSGAEGPGLQVSPESGARFGPLIKADDGGGPLV
IEADRLSGRPDLETLAEGAVEFRRGPLLLKADRVEYQQQSDLARATGNVEISRNGNIYRGPEVQLKVDRFEGYFQSPSYH
FGRTNAGGQADRFDFLDENRGIAYGATYTSCPAPDPAWLLSTDRVLLDTAAEEGTAEGAVLRFYGVPILAAPTLSFPLTE
KRKSGWLPPSIKLDNKSGFELGVPYYWDIAPNRDATLTPSVMTRRGAALDTEFRYLEPGYLGRVQAYALPDDQVANRSRW
ALNLGHEARWEGATVGEIDYGLALQRTSDDNFWKDFAGTLPSLTPRLLPTDAYARRRFDHGWGDTVAYTRVQTWQVLQDI
DPASRIVSPYQREPQIGLRQRGASGGLEWQWETEANRFSNDDPSLQTGSRLHALGSLARPWSPTGAPGWTLTPRVSFNAA
SYDLDQSLADGRRRASRVIPTVSLDSAWIFERDSTAFGRELTQTLEPRLFYVNTPYRDQTGLPNFDSAANDFNFTSIYSD
NVFSGVDRVSDANQVTAGVTTRFLDRTTGAEALRLGIAQRMLLRTQRITADGTPITQRWSDLLLLGSTNLVRNWYFDSTV
QYNSDTSRVARSISSVRYSPGPLRTLSTSYRYTRNASEQVELGWQWPLNAPAREHAAAVQARDAQMLEDFEGRSRLAPLA
GCPGAWYTVGRVNYSRRDSRITDSIVGFEYDSGCWIGRIVAERLSTGRSQATTRLLFQLELIGLSRLGSNPLKVLRENVP
GYRLLRDDSPVVAPALSTP

Sequences:

>Translated_819_residues
MSATILPTADRAATVLPPRRAWALRPVAAAVLMTTAGGGVLIDVARAQSGAEGPGLQVSPESGARFGPLIKADDGGGPLV
IEADRLSGRPDLETLAEGAVEFRRGPLLLKADRVEYQQQSDLARATGNVEISRNGNIYRGPEVQLKVDRFEGYFQSPSYH
FGRTNAGGQADRFDFLDENRGIAYGATYTSCPAPDPAWLLSTDRVLLDTAAEEGTAEGAVLRFYGVPILAAPTLSFPLTE
KRKSGWLPPSIKLDNKSGFELGVPYYWDIAPNRDATLTPSVMTRRGAALDTEFRYLEPGYLGRVQAYALPDDQVANRSRW
ALNLGHEARWEGATVGEIDYGLALQRTSDDNFWKDFAGTLPSLTPRLLPTDAYARRRFDHGWGDTVAYTRVQTWQVLQDI
DPASRIVSPYQREPQIGLRQRGASGGLEWQWETEANRFSNDDPSLQTGSRLHALGSLARPWSPTGAPGWTLTPRVSFNAA
SYDLDQSLADGRRRASRVIPTVSLDSAWIFERDSTAFGRELTQTLEPRLFYVNTPYRDQTGLPNFDSAANDFNFTSIYSD
NVFSGVDRVSDANQVTAGVTTRFLDRTTGAEALRLGIAQRMLLRTQRITADGTPITQRWSDLLLLGSTNLVRNWYFDSTV
QYNSDTSRVARSISSVRYSPGPLRTLSTSYRYTRNASEQVELGWQWPLNAPAREHAAAVQARDAQMLEDFEGRSRLAPLA
GCPGAWYTVGRVNYSRRDSRITDSIVGFEYDSGCWIGRIVAERLSTGRSQATTRLLFQLELIGLSRLGSNPLKVLRENVP
GYRLLRDDSPVVAPALSTP
>Mature_818_residues
SATILPTADRAATVLPPRRAWALRPVAAAVLMTTAGGGVLIDVARAQSGAEGPGLQVSPESGARFGPLIKADDGGGPLVI
EADRLSGRPDLETLAEGAVEFRRGPLLLKADRVEYQQQSDLARATGNVEISRNGNIYRGPEVQLKVDRFEGYFQSPSYHF
GRTNAGGQADRFDFLDENRGIAYGATYTSCPAPDPAWLLSTDRVLLDTAAEEGTAEGAVLRFYGVPILAAPTLSFPLTEK
RKSGWLPPSIKLDNKSGFELGVPYYWDIAPNRDATLTPSVMTRRGAALDTEFRYLEPGYLGRVQAYALPDDQVANRSRWA
LNLGHEARWEGATVGEIDYGLALQRTSDDNFWKDFAGTLPSLTPRLLPTDAYARRRFDHGWGDTVAYTRVQTWQVLQDID
PASRIVSPYQREPQIGLRQRGASGGLEWQWETEANRFSNDDPSLQTGSRLHALGSLARPWSPTGAPGWTLTPRVSFNAAS
YDLDQSLADGRRRASRVIPTVSLDSAWIFERDSTAFGRELTQTLEPRLFYVNTPYRDQTGLPNFDSAANDFNFTSIYSDN
VFSGVDRVSDANQVTAGVTTRFLDRTTGAEALRLGIAQRMLLRTQRITADGTPITQRWSDLLLLGSTNLVRNWYFDSTVQ
YNSDTSRVARSISSVRYSPGPLRTLSTSYRYTRNASEQVELGWQWPLNAPAREHAAAVQARDAQMLEDFEGRSRLAPLAG
CPGAWYTVGRVNYSRRDSRITDSIVGFEYDSGCWIGRIVAERLSTGRSQATTRLLFQLELIGLSRLGSNPLKVLRENVPG
YRLLRDDSPVVAPALSTP

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=776, Percent_Identity=27.0618556701031, Blast_Score=239, Evalue=4e-64,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR007543 [H]

Pfam domain/function: PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 90396; Mature: 90265

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATILPTADRAATVLPPRRAWALRPVAAAVLMTTAGGGVLIDVARAQSGAEGPGLQVSP
CCCEEECCCCCCEECCCCHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCEEECC
ESGARFGPLIKADDGGGPLVIEADRLSGRPDLETLAEGAVEFRRGPLLLKADRVEYQQQS
CCCCCCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHH
DLARATGNVEISRNGNIYRGPEVQLKVDRFEGYFQSPSYHFGRTNAGGQADRFDFLDENR
HHHHHCCCEEECCCCCEEECCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCC
GIAYGATYTSCPAPDPAWLLSTDRVLLDTAAEEGTAEGAVLRFYGVPILAAPTLSFPLTE
CEEECCCCCCCCCCCCCEEEECCEEEEECCCCCCCCCCEEEEEECCCEEECCCCCCCCCH
KRKSGWLPPSIKLDNKSGFELGVPYYWDIAPNRDATLTPSVMTRRGAALDTEFRYLEPGY
HHHCCCCCCCEEECCCCCCEECCCEEEECCCCCCCEECHHHHHHCCCCCCCCCEEECCCC
LGRVQAYALPDDQVANRSRWALNLGHEARWEGATVGEIDYGLALQRTSDDNFWKDFAGTL
CCCEEEEECCCHHHCCCCCEEEECCCCCCCCCCEECCCCCCEEEEECCCCCHHHHHHCCC
PSLTPRLLPTDAYARRRFDHGWGDTVAYTRVQTWQVLQDIDPASRIVSPYQREPQIGLRQ
CCCCCCCCCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHCCCHHHHHCCCCCCCCCCCHHH
RGASGGLEWQWETEANRFSNDDPSLQTGSRLHALGSLARPWSPTGAPGWTLTPRVSFNAA
CCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEECCEEECCCC
SYDLDQSLADGRRRASRVIPTVSLDSAWIFERDSTAFGRELTQTLEPRLFYVNTPYRDQT
CCCHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCC
GLPNFDSAANDFNFTSIYSDNVFSGVDRVSDANQVTAGVTTRFLDRTTGAEALRLGIAQR
CCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
MLLRTQRITADGTPITQRWSDLLLLGSTNLVRNWYFDSTVQYNSDTSRVARSISSVRYSP
HHHHHHHCCCCCCCHHHHHCCEEEECCCHHEEEEECCCEEEECCCHHHHHHHHHHCCCCC
GPLRTLSTSYRYTRNASEQVELGWQWPLNAPAREHAAAVQARDAQMLEDFEGRSRLAPLA
CCCHHHHCCHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GCPGAWYTVGRVNYSRRDSRITDSIVGFEYDSGCWIGRIVAERLSTGRSQATTRLLFQLE
CCCCCEEEEEECCCCCCCCHHHHHHCCEECCCCCHHHHHHHHHHHCCHHHHHHHHEEEHE
LIGLSRLGSNPLKVLRENVPGYRLLRDDSPVVAPALSTP
EHHHHHCCCCHHHHHHHCCCCEEEEECCCCEECCCCCCC
>Mature Secondary Structure 
SATILPTADRAATVLPPRRAWALRPVAAAVLMTTAGGGVLIDVARAQSGAEGPGLQVSP
CCEEECCCCCCEECCCCHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCEEECC
ESGARFGPLIKADDGGGPLVIEADRLSGRPDLETLAEGAVEFRRGPLLLKADRVEYQQQS
CCCCCCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHH
DLARATGNVEISRNGNIYRGPEVQLKVDRFEGYFQSPSYHFGRTNAGGQADRFDFLDENR
HHHHHCCCEEECCCCCEEECCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCC
GIAYGATYTSCPAPDPAWLLSTDRVLLDTAAEEGTAEGAVLRFYGVPILAAPTLSFPLTE
CEEECCCCCCCCCCCCCEEEECCEEEEECCCCCCCCCCEEEEEECCCEEECCCCCCCCCH
KRKSGWLPPSIKLDNKSGFELGVPYYWDIAPNRDATLTPSVMTRRGAALDTEFRYLEPGY
HHHCCCCCCCEEECCCCCCEECCCEEEECCCCCCCEECHHHHHHCCCCCCCCCEEECCCC
LGRVQAYALPDDQVANRSRWALNLGHEARWEGATVGEIDYGLALQRTSDDNFWKDFAGTL
CCCEEEEECCCHHHCCCCCEEEECCCCCCCCCCEECCCCCCEEEEECCCCCHHHHHHCCC
PSLTPRLLPTDAYARRRFDHGWGDTVAYTRVQTWQVLQDIDPASRIVSPYQREPQIGLRQ
CCCCCCCCCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHCCCHHHHHCCCCCCCCCCCHHH
RGASGGLEWQWETEANRFSNDDPSLQTGSRLHALGSLARPWSPTGAPGWTLTPRVSFNAA
CCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEECCEEECCCC
SYDLDQSLADGRRRASRVIPTVSLDSAWIFERDSTAFGRELTQTLEPRLFYVNTPYRDQT
CCCHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCC
GLPNFDSAANDFNFTSIYSDNVFSGVDRVSDANQVTAGVTTRFLDRTTGAEALRLGIAQR
CCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
MLLRTQRITADGTPITQRWSDLLLLGSTNLVRNWYFDSTVQYNSDTSRVARSISSVRYSP
HHHHHHHCCCCCCCHHHHHCCEEEECCCHHEEEEECCCEEEECCCHHHHHHHHHHCCCCC
GPLRTLSTSYRYTRNASEQVELGWQWPLNAPAREHAAAVQARDAQMLEDFEGRSRLAPLA
CCCHHHHCCHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GCPGAWYTVGRVNYSRRDSRITDSIVGFEYDSGCWIGRIVAERLSTGRSQATTRLLFQLE
CCCCCEEEEEECCCCCCCCHHHHHHCCEECCCCCHHHHHHHHHHHCCHHHHHHHHEEEHE
LIGLSRLGSNPLKVLRENVPGYRLLRDDSPVVAPALSTP
EHHHHHCCCCHHHHHHHCCCCEEEEECCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA