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The map label for this gene is metH [H]

Identifier: 124265359

GI number: 124265359

Start: 177474

End: 178547

Strand: Direct

Name: metH [H]

Synonym: Mpe_A0166

Alternate gene names: 124265359

Gene position: 177474-178547 (Clockwise)

Preceding gene: 124265358

Following gene: 124265361

Centisome position: 4.39

GC content: 69.27

Gene sequence:

>1074_bases
ATGAGTGCCCTGCCCTATACCCGTGCCGCCCAGTTGCCCGAGCTGCTGAAGCAGCGCATCCTGATCCTCGACGGCGCGAT
GGGCACGATGATCCAGCGCTACAAGCTCGGCGAGGCCGACTACCGCGGCGAGCGCTTCAAGGACCACGGCAAGGACCTGA
AGGGCAACAACGAGCTGCTGCAGTTCACGCGGCCCGATGTCATCACCGAGATCCACGAGGGCTACCTCGCGGCCGGCGCC
GACCTGATCGAGACCAACACCTTCGGCGCGACCACGATCGCCCAGGAAGACTACGGCCTGGCGCCGCTGGCACGCGAGAT
GAACGTGGTGGCGGCCCGGCTCGCGCGCGCCGCCTGCGACAAGTTCAGCACGCCGGACAAGCCGCGCTTCGTGGCCGGCG
CCTTCGGCCCGACGCCGCGCACCGCGAGCATCAGCCCCGATGTCAACGACCCGGCGGCGCGCAACGTCAGCTTCGACGAG
CTGCGGGCCGCGTACTACGAGCAGGGCGAGGGGCTGCTCGAGGGCGGCGCCGACCTCTTCCTGGTCGAGACCATCTTCGA
CACGCTGAACGCCAAGGCGGCGATCTTCGCGATCGACGAGCTGTTCGAGAACACCGGCGAACGCCTGCCGGTCATCATCT
CCGGCACCGTCACCGATGCGTCGGGCCGCATCCTCAGCGGCCAGACCGTCAGCGCCTTCTGGCATTCGGTGCGCCACGCG
CATCCGGTGGCCGTGGGGCTGAACTGCGCACTCGGCGCGACGCTGATGCGGCCCTACATCGAGGAGCTGTGCAAGGCCGC
GCCCGACACCTTCATCTCCTGCTACCCGAACGCCGGCCTGCCCAATCCGATGAGCGACACCGGCTTCGACGAGACCCCGG
CGATCACCGGCGGCCTGGTCGAGGAGTTCGCGCGGGCCGGCTTCCTGAACATCGCCGGCGGTTGCTGCGGCACCACCCCG
GATCACATCGCCGAGATCGCCCGGCGCGTCAGCGCCTACCGGCCGCGCGCACGGCAGGAGCGCTGGATCAGCGAGTTGAG
CGAAGAGGCCGACGGCGATCTGCAGACGGCCTGA

Upstream 100 bases:

>100_bases
GAGCACCGCGTGAAGCCGGGCGTGCGCGAGCGCCGCAGCATCGGCTTCGTGCGCTAGCTTCGTCAGTAGGGGCATCGGTC
GGCCTTCTTACAATCGGGGC

Downstream 100 bases:

>100_bases
CGGCGCGCGGCCGCCGGGCCTTCGCGCACATCGGCTGCATCACTTGCCGGCCGGGCGGTCGCTGACCAGCAGTTCCAGTC
CGGCGCCTTCGGCCCGGCCC

Product: methionine synthase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSALPYTRAAQLPELLKQRILILDGAMGTMIQRYKLGEADYRGERFKDHGKDLKGNNELLQFTRPDVITEIHEGYLAAGA
DLIETNTFGATTIAQEDYGLAPLAREMNVVAARLARAACDKFSTPDKPRFVAGAFGPTPRTASISPDVNDPAARNVSFDE
LRAAYYEQGEGLLEGGADLFLVETIFDTLNAKAAIFAIDELFENTGERLPVIISGTVTDASGRILSGQTVSAFWHSVRHA
HPVAVGLNCALGATLMRPYIEELCKAAPDTFISCYPNAGLPNPMSDTGFDETPAITGGLVEEFARAGFLNIAGGCCGTTP
DHIAEIARRVSAYRPRARQERWISELSEEADGDLQTA

Sequences:

>Translated_357_residues
MSALPYTRAAQLPELLKQRILILDGAMGTMIQRYKLGEADYRGERFKDHGKDLKGNNELLQFTRPDVITEIHEGYLAAGA
DLIETNTFGATTIAQEDYGLAPLAREMNVVAARLARAACDKFSTPDKPRFVAGAFGPTPRTASISPDVNDPAARNVSFDE
LRAAYYEQGEGLLEGGADLFLVETIFDTLNAKAAIFAIDELFENTGERLPVIISGTVTDASGRILSGQTVSAFWHSVRHA
HPVAVGLNCALGATLMRPYIEELCKAAPDTFISCYPNAGLPNPMSDTGFDETPAITGGLVEEFARAGFLNIAGGCCGTTP
DHIAEIARRVSAYRPRARQERWISELSEEADGDLQTA
>Mature_356_residues
SALPYTRAAQLPELLKQRILILDGAMGTMIQRYKLGEADYRGERFKDHGKDLKGNNELLQFTRPDVITEIHEGYLAAGAD
LIETNTFGATTIAQEDYGLAPLAREMNVVAARLARAACDKFSTPDKPRFVAGAFGPTPRTASISPDVNDPAARNVSFDEL
RAAYYEQGEGLLEGGADLFLVETIFDTLNAKAAIFAIDELFENTGERLPVIISGTVTDASGRILSGQTVSAFWHSVRHAH
PVAVGLNCALGATLMRPYIEELCKAAPDTFISCYPNAGLPNPMSDTGFDETPAITGGLVEEFARAGFLNIAGGCCGTTPD
HIAEIARRVSAYRPRARQERWISELSEEADGDLQTA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=335, Percent_Identity=54.0298507462687, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI1790450, Length=332, Percent_Identity=56.9277108433735, Blast_Score=372, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17535405, Length=329, Percent_Identity=55.0151975683891, Blast_Score=358, Evalue=2e-99,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 38603; Mature: 38472

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALPYTRAAQLPELLKQRILILDGAMGTMIQRYKLGEADYRGERFKDHGKDLKGNNELL
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHE
QFTRPDVITEIHEGYLAAGADLIETNTFGATTIAQEDYGLAPLAREMNVVAARLARAACD
EECCCHHHHHHHHHHHHCCCHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
KFSTPDKPRFVAGAFGPTPRTASISPDVNDPAARNVSFDELRAAYYEQGEGLLEGGADLF
HCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCEE
LVETIFDTLNAKAAIFAIDELFENTGERLPVIISGTVTDASGRILSGQTVSAFWHSVRHA
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEECCCHHHHHHHHHHHC
HPVAVGLNCALGATLMRPYIEELCKAAPDTFISCYPNAGLPNPMSDTGFDETPAITGGLV
CCCEEECHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHH
EEFARAGFLNIAGGCCGTTPDHIAEIARRVSAYRPRARQERWISELSEEADGDLQTA
HHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SALPYTRAAQLPELLKQRILILDGAMGTMIQRYKLGEADYRGERFKDHGKDLKGNNELL
CCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHE
QFTRPDVITEIHEGYLAAGADLIETNTFGATTIAQEDYGLAPLAREMNVVAARLARAACD
EECCCHHHHHHHHHHHHCCCHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
KFSTPDKPRFVAGAFGPTPRTASISPDVNDPAARNVSFDELRAAYYEQGEGLLEGGADLF
HCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCEE
LVETIFDTLNAKAAIFAIDELFENTGERLPVIISGTVTDASGRILSGQTVSAFWHSVRHA
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEECCCHHHHHHHHHHHC
HPVAVGLNCALGATLMRPYIEELCKAAPDTFISCYPNAGLPNPMSDTGFDETPAITGGLV
CCCEEECHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHH
EEFARAGFLNIAGGCCGTTPDHIAEIARRVSAYRPRARQERWISELSEEADGDLQTA
HHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]