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The map label for this gene is pyrF [H]

Identifier: 124022272

GI number: 124022272

Start: 543286

End: 544026

Strand: Direct

Name: pyrF [H]

Synonym: P9303_05621

Alternate gene names: 124022272

Gene position: 543286-544026 (Clockwise)

Preceding gene: 124022271

Following gene: 124022276

Centisome position: 20.25

GC content: 58.03

Gene sequence:

>741_bases
GTGATGACCTCGCCATTGACTCCTGCCGATCAACTGATCATTGCTCTTGATGGCATGGAGCGGGCTGAGGCCCTTGCATT
CATCTCAAAGTTGCCAGAGGTCAGTTGGGTCAAGGTGGGCCTGGAACTGTTTGTTAGTGCGGGGCCAGAGGTTTTGGCTG
ATCTCAGGGAGCAAGGGTTGCGGATATTTCTTGATCTCAAGTTTCACGATATCCCTGCCACCATGGCAGGAGCTTGTCGC
CGAGCGGCGAGCTTTGGCGCTGAGTTGATCACGGTGCATGCCTGTGCTGGCCGTGCTGCCCTTGATGAAGCTCAGACTGC
TGCTTGCGAGGGGGCTGCGGAGGTTGGCTTGTCAGCACCCCGGCTGTTGGCCGTCACTGTGCTCACCAGTTGGGATCAGA
AGCGTCTAGCGAATGAGCTGTGCATTCCTCAGTCTCTTCAGGCCAGGGTGGAGTGGTTGGCTGAGTTGGCAGCGGAGTCA
GGGTTAGGCGGCTGCGTGTGTTCTCCCTGGGAAGTAAGCGGCTTACGCCGACTTCATCCCTCGCCATTTGAGTTAGTGAC
TCCTGGCATTCGCCCCTCGGGGGCTGAATTGGCGGATCAGGTCAGGGTCATGAGTCCTGCAGCGGCGCTGAATGCAGGAG
CTTCCCGTTTGGTAATCGGCAGGCCGATTACCCGTGCTGTTGATCCGGCAGAGGCGTTTGCACGCTGCTGCTTTGAGATT
GAACAGCGTCAGCTGGACTGA

Upstream 100 bases:

>100_bases
TGGAATTCACTGACTCCAGCCTGCTGATGGGCAAGGTGCTGCAAGTGGGCAAGAAAACCTTTCGTCGTTTGACGCGTTGA
GTAAAGGACCAACCCCTGCT

Downstream 100 bases:

>100_bases
CCGAGGCGAGGTTCGGTGCCCGCCAGCAAGCGTTGTAGATTGGAGCGGTGTCGCCACAACACCATTGCCATGGCGGCAAA
GGCCACCGCCAGGTAAGCCG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MMTSPLTPADQLIIALDGMERAEALAFISKLPEVSWVKVGLELFVSAGPEVLADLREQGLRIFLDLKFHDIPATMAGACR
RAASFGAELITVHACAGRAALDEAQTAACEGAAEVGLSAPRLLAVTVLTSWDQKRLANELCIPQSLQARVEWLAELAAES
GLGGCVCSPWEVSGLRRLHPSPFELVTPGIRPSGAELADQVRVMSPAAALNAGASRLVIGRPITRAVDPAEAFARCCFEI
EQRQLD

Sequences:

>Translated_246_residues
MMTSPLTPADQLIIALDGMERAEALAFISKLPEVSWVKVGLELFVSAGPEVLADLREQGLRIFLDLKFHDIPATMAGACR
RAASFGAELITVHACAGRAALDEAQTAACEGAAEVGLSAPRLLAVTVLTSWDQKRLANELCIPQSLQARVEWLAELAAES
GLGGCVCSPWEVSGLRRLHPSPFELVTPGIRPSGAELADQVRVMSPAAALNAGASRLVIGRPITRAVDPAEAFARCCFEI
EQRQLD
>Mature_246_residues
MMTSPLTPADQLIIALDGMERAEALAFISKLPEVSWVKVGLELFVSAGPEVLADLREQGLRIFLDLKFHDIPATMAGACR
RAASFGAELITVHACAGRAALDEAQTAACEGAAEVGLSAPRLLAVTVLTSWDQKRLANELCIPQSLQARVEWLAELAAES
GLGGCVCSPWEVSGLRRLHPSPFELVTPGIRPSGAELADQVRVMSPAAALNAGASRLVIGRPITRAVDPAEAFARCCFEI
EQRQLD

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=223, Percent_Identity=44.3946188340807, Blast_Score=168, Evalue=3e-43,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 26196; Mature: 26196

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTSPLTPADQLIIALDGMERAEALAFISKLPEVSWVKVGLELFVSAGPEVLADLREQGL
CCCCCCCCHHHEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCC
RIFLDLKFHDIPATMAGACRRAASFGAELITVHACAGRAALDEAQTAACEGAAEVGLSAP
EEEEEEEECCCCHHHHHHHHHHHHCCHHHEEEEHHCCCHHHHHHHHHHHCCHHHHCCCCC
RLLAVTVLTSWDQKRLANELCIPQSLQARVEWLAELAAESGLGGCVCSPWEVSGLRRLHP
HHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHCCC
SPFELVTPGIRPSGAELADQVRVMSPAAALNAGASRLVIGRPITRAVDPAEAFARCCFEI
CCHHHCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHH
EQRQLD
HHHCCC
>Mature Secondary Structure
MMTSPLTPADQLIIALDGMERAEALAFISKLPEVSWVKVGLELFVSAGPEVLADLREQGL
CCCCCCCCHHHEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCC
RIFLDLKFHDIPATMAGACRRAASFGAELITVHACAGRAALDEAQTAACEGAAEVGLSAP
EEEEEEEECCCCHHHHHHHHHHHHCCHHHEEEEHHCCCHHHHHHHHHHHCCHHHHCCCCC
RLLAVTVLTSWDQKRLANELCIPQSLQARVEWLAELAAESGLGGCVCSPWEVSGLRRLHP
HHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHCCC
SPFELVTPGIRPSGAELADQVRVMSPAAALNAGASRLVIGRPITRAVDPAEAFARCCFEI
CCHHHCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHH
EQRQLD
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]