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The map label for this gene is spt [H]

Identifier: 124025481

GI number: 124025481

Start: 706712

End: 707950

Strand: Direct

Name: spt [H]

Synonym: NATL1_07741

Alternate gene names: 124025481

Gene position: 706712-707950 (Clockwise)

Preceding gene: 124025477

Following gene: 124025483

Centisome position: 37.9

GC content: 38.01

Gene sequence:

>1239_bases
TTGCAATCCCTAAAAAAAACACCCCGACTTTGCCTATCGCCTTTTAGGCTCGGAAGATATATAAACCGATCTGTTTTCTT
GGCCACTCAAATTCTTCCTTCTGTTGATAATCAACATCGTAAAGATTTTGGTCCAACTGTTTCTCCTGAAAGGTTATTGC
TTGGGCCAGGCCCATCAAACGCAGATCCTGCTGTTTTGAAAGCCCTCTCTCAACCTCCTATTGGTCATTTAGATCCTTTT
TATGTTGACTTGATGAGTGAGGTACAAGAGTTGCTTAGATATGCTTGGCAAACTAGCAATCGATTAACACTTCCAATGAG
TGGCACAGGAAGTGCCGCAATGGAGGCAACATTAGCAAATGTCGTTGAGCCAGAAGATACGGTATTAATAGCTATCAAAG
GATATTTTGGACACCGACTTGCGGATATGGCAGGTAGGTATAAAGCGAATGTAGAAACAATTCATAAAGAATGGGGTAAT
GCATTTTCACTTCAGGAAATAGAAGATGCATTGAAAAAACATACCCCAGCTGTTTTAGCAATTGTTCATGCGGAAACTTC
AACAGGTGTATGTCAACCTATGGATGGCATTGGTGATTTATGCAGAAAATATAATTGCTTGCTTCTTGTAGACACAGTAA
CTTCTTTGGGAGGCGTTCCTCTTTATTTGGATGAATGGAAAATTGATCTGGCTTATAGTTGCAGTCAGAAAGGATTAAGT
TGCCCTCCTGGATTAGGTCCTTTTTCAATGAATGAGAGAGCCGAAAATAAAATGTCAAATAGAAAAGAAAAAGTACCAAA
TTGGTATTTAGATGTCTCTTTGCTTAATAAATATTGGGGCAGTGATCGTGTATATCATCATACGGCTCCCGTAAATATGA
ATTTTGGCATTAGAGAAGCGCTTAGATTGTTAGCGGAAGAAGGATTAGAAGTCTCATGGGGTAGACATCGTACAAATGCA
AAATCGCTTTGGAATTCGTTGGAAAATATAGGTTTGGAATTACACGTTAAAGAAGAATTACGCTTACCTACCCTAACAAC
AGTGAAAATACCTGAGGGAGTTGATGGTAAGGCATTTACTAAGCATTTACTAAATAATTTTGGAGTTGAAATAGGAGGTG
GATTAGGTGATCTGGCTGGAAAAGTATGGAGAATTGGTTTAATGGGATATAACTCCACCTCTGAAAATGTAGATAAGATA
ATAAATATTTTTGAAACTGAATTACCTAAGTTTAGATAA

Upstream 100 bases:

>100_bases
CGATCGCATCTCTTTTTGTATTAAGACGCACTTTTTTTAGACTTTTAACAGAAAACTTTGTTTATTTTTTGTCTTTTTTT
GTAAATCTAGCAAATTCCTA

Downstream 100 bases:

>100_bases
CAAATTTTCTCTGGCTATTAATCTTTTATCTTTAAACCAATCCACTATAATCTTCTTAGATTCTTCTTCCATGATTCCTT
TTTCAATCAACAGTCTGTGA

Product: serine:pyruvate/alanine:glyoxylate aminotransferase

Products: pyruvate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MQSLKKTPRLCLSPFRLGRYINRSVFLATQILPSVDNQHRKDFGPTVSPERLLLGPGPSNADPAVLKALSQPPIGHLDPF
YVDLMSEVQELLRYAWQTSNRLTLPMSGTGSAAMEATLANVVEPEDTVLIAIKGYFGHRLADMAGRYKANVETIHKEWGN
AFSLQEIEDALKKHTPAVLAIVHAETSTGVCQPMDGIGDLCRKYNCLLLVDTVTSLGGVPLYLDEWKIDLAYSCSQKGLS
CPPGLGPFSMNERAENKMSNRKEKVPNWYLDVSLLNKYWGSDRVYHHTAPVNMNFGIREALRLLAEEGLEVSWGRHRTNA
KSLWNSLENIGLELHVKEELRLPTLTTVKIPEGVDGKAFTKHLLNNFGVEIGGGLGDLAGKVWRIGLMGYNSTSENVDKI
INIFETELPKFR

Sequences:

>Translated_412_residues
MQSLKKTPRLCLSPFRLGRYINRSVFLATQILPSVDNQHRKDFGPTVSPERLLLGPGPSNADPAVLKALSQPPIGHLDPF
YVDLMSEVQELLRYAWQTSNRLTLPMSGTGSAAMEATLANVVEPEDTVLIAIKGYFGHRLADMAGRYKANVETIHKEWGN
AFSLQEIEDALKKHTPAVLAIVHAETSTGVCQPMDGIGDLCRKYNCLLLVDTVTSLGGVPLYLDEWKIDLAYSCSQKGLS
CPPGLGPFSMNERAENKMSNRKEKVPNWYLDVSLLNKYWGSDRVYHHTAPVNMNFGIREALRLLAEEGLEVSWGRHRTNA
KSLWNSLENIGLELHVKEELRLPTLTTVKIPEGVDGKAFTKHLLNNFGVEIGGGLGDLAGKVWRIGLMGYNSTSENVDKI
INIFETELPKFR
>Mature_412_residues
MQSLKKTPRLCLSPFRLGRYINRSVFLATQILPSVDNQHRKDFGPTVSPERLLLGPGPSNADPAVLKALSQPPIGHLDPF
YVDLMSEVQELLRYAWQTSNRLTLPMSGTGSAAMEATLANVVEPEDTVLIAIKGYFGHRLADMAGRYKANVETIHKEWGN
AFSLQEIEDALKKHTPAVLAIVHAETSTGVCQPMDGIGDLCRKYNCLLLVDTVTSLGGVPLYLDEWKIDLAYSCSQKGLS
CPPGLGPFSMNERAENKMSNRKEKVPNWYLDVSLLNKYWGSDRVYHHTAPVNMNFGIREALRLLAEEGLEVSWGRHRTNA
KSLWNSLENIGLELHVKEELRLPTLTTVKIPEGVDGKAFTKHLLNNFGVEIGGGLGDLAGKVWRIGLMGYNSTSENVDKI
INIFETELPKFR

Specific function: Could encode ureidoglycolase or L-alanine:glyoxylate aminotransferase, or perhaps both activities [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=368, Percent_Identity=46.195652173913, Blast_Score=339, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI17536281, Length=366, Percent_Identity=42.3497267759563, Blast_Score=317, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6321079, Length=378, Percent_Identity=27.5132275132275, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI17530823, Length=365, Percent_Identity=44.1095890410959, Blast_Score=322, Evalue=4e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.44

Molecular weight: Translated: 45810; Mature: 45810

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLKKTPRLCLSPFRLGRYINRSVFLATQILPSVDNQHRKDFGPTVSPERLLLGPGPSN
CCCHHHCHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEEEECCCCCC
ADPAVLKALSQPPIGHLDPFYVDLMSEVQELLRYAWQTSNRLTLPMSGTGSAAMEATLAN
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
VVEPEDTVLIAIKGYFGHRLADMAGRYKANVETIHKEWGNAFSLQEIEDALKKHTPAVLA
HCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEE
IVHAETSTGVCQPMDGIGDLCRKYNCLLLVDTVTSLGGVPLYLDEWKIDLAYSCSQKGLS
EEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEHHHHCCCEEEEECEEEEEEEECCCCCCC
CPPGLGPFSMNERAENKMSNRKEKVPNWYLDVSLLNKYWGSDRVYHHTAPVNMNFGIREA
CCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCCCEEEECCCCCCCCCHHHH
LRLLAEEGLEVSWGRHRTNAKSLWNSLENIGLELHVKEELRLPTLTTVKIPEGVDGKAFT
HHHHHHCCCEECCCCCCCHHHHHHHHHHHCCEEEEEHHHCCCCCEEEEECCCCCCHHHHH
KHLLNNFGVEIGGGLGDLAGKVWRIGLMGYNSTSENVDKIINIFETELPKFR
HHHHHHCCEEECCCHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQSLKKTPRLCLSPFRLGRYINRSVFLATQILPSVDNQHRKDFGPTVSPERLLLGPGPSN
CCCHHHCHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEEEECCCCCC
ADPAVLKALSQPPIGHLDPFYVDLMSEVQELLRYAWQTSNRLTLPMSGTGSAAMEATLAN
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
VVEPEDTVLIAIKGYFGHRLADMAGRYKANVETIHKEWGNAFSLQEIEDALKKHTPAVLA
HCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEE
IVHAETSTGVCQPMDGIGDLCRKYNCLLLVDTVTSLGGVPLYLDEWKIDLAYSCSQKGLS
EEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEHHHHCCCEEEEECEEEEEEEECCCCCCC
CPPGLGPFSMNERAENKMSNRKEKVPNWYLDVSLLNKYWGSDRVYHHTAPVNMNFGIREA
CCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCCCEEEECCCCCCCCCHHHH
LRLLAEEGLEVSWGRHRTNAKSLWNSLENIGLELHVKEELRLPTLTTVKIPEGVDGKAFT
HHHHHHCCCEECCCCCCCHHHHHHHHHHHCCEEEEEHHHCCCCCEEEEECCCCCCHHHHH
KHLLNNFGVEIGGGLGDLAGKVWRIGLMGYNSTSENVDKIINIFETELPKFR
HHHHHHCCEEECCCHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-alanine; glyoxylate

Specific reaction: L-alanine + glyoxylate = pyruvate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]