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The map label for this gene is rsmE [H]

Identifier: 124025432

GI number: 124025432

Start: 668425

End: 669189

Strand: Direct

Name: rsmE [H]

Synonym: NATL1_07251

Alternate gene names: 124025432

Gene position: 668425-669189 (Clockwise)

Preceding gene: 124025431

Following gene: 124025433

Centisome position: 35.85

GC content: 35.29

Gene sequence:

>765_bases
ATGGCTGAGAAAAGAAGATTATTTATAAAACTAAATCGATTAAGAGATGAAATCGATTCAAATGGAGAGTTGAAATTGAC
CTCTAATGAATCACATTATTTATTCAGGGTTATGCGGATGAGATCGGAAGATCTTTTAGAAGTCATTGATGGAAAAGGTC
GTCTTTGGAACGCAAAAATTGTTGAAAAAAAAGCTATAAAACTTACCAATGGTTTTGATAAGCCTCTTCAATTCGTTTCA
AGAGAAAGACCATTAATAGGAATTGCCATAGTTATTCCTAAAAAGGGGTTTGAAAACTTTTTGCAAATGAGTTGTGAGAT
AGGAGTTGATATTATTCAACCATTAATCTCAAAACGATCAGTAGTTAAAGAATGCAGTAACGAAAAAATTATTAGATATA
AAAAAATTATTTATGAAGCCGTTGAGCAATCCGAAAGACTCTGGAGTCCAGAATTAATGCAGGTATTAACATTTCCCAAT
TGGATAAATGATATGTCTTCGGAAGCTCAAGTCGGATTTGCGGCAACACGTATTGAAGACTTGCCAGATTGTGTGACTTG
GGTAAAAGAAACACCACATGAAGTCAATCAAGTTTGGGTGGTAATTGGACCAGAGGGAGGATGGAATAAAGAAGAAGAGG
AATTAGCTTTTAACGCTGGATTCGTTGGGGTGTCTATGGGAGAAACTATTTTGAGGACATCAACAGCTGCCGTAAGTGCC
TTACAAGTAATGACTTCATGGAGAAGACTTAAATCATCCTTTTAA

Upstream 100 bases:

>100_bases
TGTTTTGAAAATAGAGGAACTAATTATAGGAGGGGTCAATGAAGATTGGCCAGTAGAGGAACATCCAGATGCAATTTTTT
CTTCAACAGATTAATTTTCT

Downstream 100 bases:

>100_bases
GATTCAATATTTTTGATCATAAAAACGTCTATTGTTTATAAACAGGCATGCTTAGTAGAAACCGATGGGTAACTTGTCTT
TTGTTGGGGGTACTTGGGTT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MAEKRRLFIKLNRLRDEIDSNGELKLTSNESHYLFRVMRMRSEDLLEVIDGKGRLWNAKIVEKKAIKLTNGFDKPLQFVS
RERPLIGIAIVIPKKGFENFLQMSCEIGVDIIQPLISKRSVVKECSNEKIIRYKKIIYEAVEQSERLWSPELMQVLTFPN
WINDMSSEAQVGFAATRIEDLPDCVTWVKETPHEVNQVWVVIGPEGGWNKEEEELAFNAGFVGVSMGETILRTSTAAVSA
LQVMTSWRRLKSSF

Sequences:

>Translated_254_residues
MAEKRRLFIKLNRLRDEIDSNGELKLTSNESHYLFRVMRMRSEDLLEVIDGKGRLWNAKIVEKKAIKLTNGFDKPLQFVS
RERPLIGIAIVIPKKGFENFLQMSCEIGVDIIQPLISKRSVVKECSNEKIIRYKKIIYEAVEQSERLWSPELMQVLTFPN
WINDMSSEAQVGFAATRIEDLPDCVTWVKETPHEVNQVWVVIGPEGGWNKEEEELAFNAGFVGVSMGETILRTSTAAVSA
LQVMTSWRRLKSSF
>Mature_253_residues
AEKRRLFIKLNRLRDEIDSNGELKLTSNESHYLFRVMRMRSEDLLEVIDGKGRLWNAKIVEKKAIKLTNGFDKPLQFVSR
ERPLIGIAIVIPKKGFENFLQMSCEIGVDIIQPLISKRSVVKECSNEKIIRYKKIIYEAVEQSERLWSPELMQVLTFPNW
INDMSSEAQVGFAATRIEDLPDCVTWVKETPHEVNQVWVVIGPEGGWNKEEEELAFNAGFVGVSMGETILRTSTAAVSAL
QVMTSWRRLKSSF

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=238, Percent_Identity=27.7310924369748, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001206
- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 29151; Mature: 29020

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS00019 ACTININ_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKRRLFIKLNRLRDEIDSNGELKLTSNESHYLFRVMRMRSEDLLEVIDGKGRLWNAKI
CCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEECHHH
VEKKAIKLTNGFDKPLQFVSRERPLIGIAIVIPKKGFENFLQMSCEIGVDIIQPLISKRS
HHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHH
VVKECSNEKIIRYKKIIYEAVEQSERLWSPELMQVLTFPNWINDMSSEAQVGFAATRIED
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHH
LPDCVTWVKETPHEVNQVWVVIGPEGGWNKEEEELAFNAGFVGVSMGETILRTSTAAVSA
HHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCEEEHHHCHHHHHHHHHHHHH
LQVMTSWRRLKSSF
HHHHHHHHHHHHCC
>Mature Secondary Structure 
AEKRRLFIKLNRLRDEIDSNGELKLTSNESHYLFRVMRMRSEDLLEVIDGKGRLWNAKI
CCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEECHHH
VEKKAIKLTNGFDKPLQFVSRERPLIGIAIVIPKKGFENFLQMSCEIGVDIIQPLISKRS
HHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHH
VVKECSNEKIIRYKKIIYEAVEQSERLWSPELMQVLTFPNWINDMSSEAQVGFAATRIED
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHH
LPDCVTWVKETPHEVNQVWVVIGPEGGWNKEEEELAFNAGFVGVSMGETILRTSTAAVSA
HHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCEEEHHHCHHHHHHHHHHHHH
LQVMTSWRRLKSSF
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]