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The map label for this gene is trmH [H]

Identifier: 124025191

GI number: 124025191

Start: 435009

End: 435698

Strand: Reverse

Name: trmH [H]

Synonym: NATL1_04781

Alternate gene names: 124025191

Gene position: 435698-435009 (Counterclockwise)

Preceding gene: 124025192

Following gene: 124025189

Centisome position: 23.37

GC content: 36.96

Gene sequence:

>690_bases
ATGCCTCTACTACCTAGACGTTTTGAACGTCTAAAATCAGTATTAAATAGGAGGATTTCAGATCTCACAGTCTTAATTGA
GAATGTTGAAAAGCCTCATAATCTCTCAGCGATAATACGAAGTTGTGATGCTGTTGGTGTTCTTGAAGCTTATGCAATCT
TCAATAAGGAAAAGTTTTTAACATTTAATAGCACTGCTCAAGGAAGTCAAAAATGGGTCAAAATAAACCAATATAAAATG
ACCACCGAGGCGATAAAAGTTCTTAAAGAAAAAGGGTTTAAATTGTACGGAACGAACTTGAATCCCAGATCAATTGATTA
CAGAAAATGTAACTTCAAGGGACCAACAGCATTTGTACTAGGTGCCGAGAAATGGGGTATCAGTGAAGAAGCTTCGAGTT
TAATGGATGAGCATATTCATATTCCAATGAGAGGGATGGTTGAATCTTTAAATGTATCAGTTGCGGCATCGGCATTATTG
TTTGAAGCGATAAGACAACGACAAGTAGCCAATATGGTTCCTGAATCTGGGGAAGGCATGAGCCAAGAAACATACAAAGA
AAAGCTTTTCGAGTGGGCTTACCCCGAGGTTGCTCAATGGAGTAAAAGTGAAGGCAGAAAATATCCAGAACTTAATGATA
AAGGTGAAATTATAGATAATCTCCCAAGAACAGAAAAAATGAGATATTAA

Upstream 100 bases:

>100_bases
TGATCAAATTTTTTTTCAAATAACTAATAGGTATTTTTTATATTAAATAAAAACCACTATCCTGATCAAAGAGTAACAAC
TGTCCCATAACATATTAGAG

Downstream 100 bases:

>100_bases
ATTCCAAACCAGAAGTAAGTTTTAATCCTGAAGACTAGTTTCACTTAAAATTTTCTCCAAGCCTTCTCCAATAAAAGACA
GTCCCAGCACTAACACGAAC

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MPLLPRRFERLKSVLNRRISDLTVLIENVEKPHNLSAIIRSCDAVGVLEAYAIFNKEKFLTFNSTAQGSQKWVKINQYKM
TTEAIKVLKEKGFKLYGTNLNPRSIDYRKCNFKGPTAFVLGAEKWGISEEASSLMDEHIHIPMRGMVESLNVSVAASALL
FEAIRQRQVANMVPESGEGMSQETYKEKLFEWAYPEVAQWSKSEGRKYPELNDKGEIIDNLPRTEKMRY

Sequences:

>Translated_229_residues
MPLLPRRFERLKSVLNRRISDLTVLIENVEKPHNLSAIIRSCDAVGVLEAYAIFNKEKFLTFNSTAQGSQKWVKINQYKM
TTEAIKVLKEKGFKLYGTNLNPRSIDYRKCNFKGPTAFVLGAEKWGISEEASSLMDEHIHIPMRGMVESLNVSVAASALL
FEAIRQRQVANMVPESGEGMSQETYKEKLFEWAYPEVAQWSKSEGRKYPELNDKGEIIDNLPRTEKMRY
>Mature_228_residues
PLLPRRFERLKSVLNRRISDLTVLIENVEKPHNLSAIIRSCDAVGVLEAYAIFNKEKFLTFNSTAQGSQKWVKINQYKMT
TEAIKVLKEKGFKLYGTNLNPRSIDYRKCNFKGPTAFVLGAEKWGISEEASSLMDEHIHIPMRGMVESLNVSVAASALLF
EAIRQRQVANMVPESGEGMSQETYKEKLFEWAYPEVAQWSKSEGRKYPELNDKGEIIDNLPRTEKMRY

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI110825988, Length=171, Percent_Identity=25.7309941520468, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1790083, Length=213, Percent_Identity=42.7230046948357, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI1790623, Length=148, Percent_Identity=35.8108108108108, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 26270; Mature: 26138

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLPRRFERLKSVLNRRISDLTVLIENVEKPHNLSAIIRSCDAVGVLEAYAIFNKEKFL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCEEE
TFNSTAQGSQKWVKINQYKMTTEAIKVLKEKGFKLYGTNLNPRSIDYRKCNFKGPTAFVL
EECCCCCCCHHEEEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEECCCCCCEEEEE
GAEKWGISEEASSLMDEHIHIPMRGMVESLNVSVAASALLFEAIRQRQVANMVPESGEGM
ECHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SQETYKEKLFEWAYPEVAQWSKSEGRKYPELNDKGEIIDNLPRTEKMRY
CHHHHHHHHHHHCCHHHHHHCHHCCCCCCCCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure 
PLLPRRFERLKSVLNRRISDLTVLIENVEKPHNLSAIIRSCDAVGVLEAYAIFNKEKFL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCEEE
TFNSTAQGSQKWVKINQYKMTTEAIKVLKEKGFKLYGTNLNPRSIDYRKCNFKGPTAFVL
EECCCCCCCHHEEEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEECCCCCCEEEEE
GAEKWGISEEASSLMDEHIHIPMRGMVESLNVSVAASALLFEAIRQRQVANMVPESGEGM
ECHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SQETYKEKLFEWAYPEVAQWSKSEGRKYPELNDKGEIIDNLPRTEKMRY
CHHHHHHHHHHHCCHHHHHHCHHCCCCCCCCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]