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The map label for this gene is ndhK

Identifier: 124025086

GI number: 124025086

Start: 346294

End: 347043

Strand: Reverse

Name: ndhK

Synonym: NATL1_03731

Alternate gene names: 124025086

Gene position: 347043-346294 (Counterclockwise)

Preceding gene: 124025087

Following gene: 124025085

Centisome position: 18.61

GC content: 38.4

Gene sequence:

>750_bases
ATGGAGTTGAATCCCTTGAAAAAATCTCCCTCTTCTGAAGCTGTAAGAAATCTTAGAGAAGCTTCCTGCGGTCCAGTTGG
AACGCCGACAGTAACGAATGATTTAAGCGAAAACATAATCCTCACTAGCCTTGAAGATCTTCACAACTGGGCAAGATTAA
GTAGTCTTTGGCCTCTTCTTTATGGAACTGCTTGTTGTTTTATTGAATTTGCAGCTCTAATTGGATCGAGATTTGATTTT
GACAGATTTGGATTAGTACCTAGAAGTTCTCCTAGACAAGCAGATCTATTAATTGTGGCAGGCACTGTCACAATGAAAAT
GGCACCTGCTCTTGTAAGGCTTTATGAGCAAATGCCAGATCCCAAGTACGTAATTGCTATGGGAGCTTGCACCATTACCG
GTGGAATGTTCAGCGCAGACTCCACCACGGCAGTCAGGGGAGTTGATAAATTAATACCTGTTGATCTTTACCTTCCTGGA
TGTCCACCAAGACCTGAAGCAATATTTGATGCTGTCATTAAATTAAGAAAAAAAGTTGCAAACGAATCAATCTCAGAAAG
ATCAAAAATTACCCAAACTCATCGTTATTTAACTATCCCTCATAAAATGAAGCGAGTCGAGGCAAAAGTAAATGGACAAT
ACCTAAAGGCAAAAACTCAATTAATTGCTTTGAATCCTTCACTATCTGAAGAGTTTGATCAATCTTTAAAAGAGGTTCAA
AAAATATCTGAAGAACTTTCAAGTAATTAA

Upstream 100 bases:

>100_bases
CATTTCACAAACTTGGGTTATTAGCATTCATAGAAGCTCTTGTTTTTATCACAATACTAGTTGTGGCTTTAGCATACGCA
TGGAGAAAAGGTGCCCTTGA

Downstream 100 bases:

>100_bases
GCTTCTCTTATAAAAAATGACAAACGATTCGGAAATTGTAGTTGAAGAAAAAATCTCAGGTCCAATAAGCGATTGGCTCT
CTAACAAAGGATTTGAAAAC

Product: NADH dehydrogenase subunit B

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K; NDH-1 subunit K; NDH-K

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MELNPLKKSPSSEAVRNLREASCGPVGTPTVTNDLSENIILTSLEDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDF
DRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPDPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPG
CPPRPEAIFDAVIKLRKKVANESISERSKITQTHRYLTIPHKMKRVEAKVNGQYLKAKTQLIALNPSLSEEFDQSLKEVQ
KISEELSSN

Sequences:

>Translated_249_residues
MELNPLKKSPSSEAVRNLREASCGPVGTPTVTNDLSENIILTSLEDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDF
DRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPDPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPG
CPPRPEAIFDAVIKLRKKVANESISERSKITQTHRYLTIPHKMKRVEAKVNGQYLKAKTQLIALNPSLSEEFDQSLKEVQ
KISEELSSN
>Mature_249_residues
MELNPLKKSPSSEAVRNLREASCGPVGTPTVTNDLSENIILTSLEDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDF
DRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPDPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPG
CPPRPEAIFDAVIKLRKKVANESISERSKITQTHRYLTIPHKMKRVEAKVNGQYLKAKTQLIALNPSLSEEFDQSLKEVQ
KISEELSSN

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=143, Percent_Identity=51.0489510489511, Blast_Score=175, Evalue=3e-44,
Organism=Escherichia coli, GI1788624, Length=156, Percent_Identity=47.4358974358974, Blast_Score=165, Evalue=2e-42,
Organism=Escherichia coli, GI1789074, Length=106, Percent_Identity=44.3396226415094, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788834, Length=143, Percent_Identity=36.3636363636364, Blast_Score=94, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=175, Percent_Identity=45.7142857142857, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI18859983, Length=170, Percent_Identity=48.8235294117647, Blast_Score=177, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24642371, Length=170, Percent_Identity=48.8235294117647, Blast_Score=177, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24651058, Length=136, Percent_Identity=53.6764705882353, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NDHK_PROM1 (A2C0C7)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014202.1
- ProteinModelPortal:   A2C0C7
- SMR:   A2C0C7
- STRING:   A2C0C7
- GeneID:   4779522
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_03731
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   ITMKMAP
- ProtClustDB:   PRK06411
- BioCyc:   PMAR167555:NATL1_03731-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: 1.6.5.3

Molecular weight: Translated: 27527; Mature: 27527

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNPLKKSPSSEAVRNLREASCGPVGTPTVTNDLSENIILTSLEDLHNWARLSSLWPLL
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
YGTACCFIEFAALIGSRFDFDRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPD
HHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCC
PKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEAIFDAVIKLRKKVA
CCEEEEECHHHHCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHH
NESISERSKITQTHRYLTIPHKMKRVEAKVNGQYLKAKTQLIALNPSLSEEFDQSLKEVQ
HHHHHHHHHHHHHHHEEECHHHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHHHHH
KISEELSSN
HHHHHHCCC
>Mature Secondary Structure
MELNPLKKSPSSEAVRNLREASCGPVGTPTVTNDLSENIILTSLEDLHNWARLSSLWPLL
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
YGTACCFIEFAALIGSRFDFDRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPD
HHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCC
PKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEAIFDAVIKLRKKVA
CCEEEEECHHHHCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHH
NESISERSKITQTHRYLTIPHKMKRVEAKVNGQYLKAKTQLIALNPSLSEEFDQSLKEVQ
HHHHHHHHHHHHHHHEEECHHHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHHHHH
KISEELSSN
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA