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The map label for this gene is ndhJ

Identifier: 124025085

GI number: 124025085

Start: 345756

End: 346277

Strand: Reverse

Name: ndhJ

Synonym: NATL1_03721

Alternate gene names: 124025085

Gene position: 346277-345756 (Counterclockwise)

Preceding gene: 124025086

Following gene: 124025083

Centisome position: 18.57

GC content: 34.48

Gene sequence:

>522_bases
ATGACAAACGATTCGGAAATTGTAGTTGAAGAAAAAATCTCAGGTCCAATAAGCGATTGGCTCTCTAACAAAGGATTTGA
AAACATTCCTCTCAAAGAAGACCACTTAGGAATTGAAGTTATAAAAATCTCGCCAAACAATCTCTTATCAATAGTTGAAG
CGTTAAAAAATGATGGCTTTAATTATCTGCAATGCCAAGGTGGCTATGACGAAGGTCCTGGTTTAAATATAGTTTGTTTT
TATAATCTAATAGAAATGAATGAATTAAAAGAAGATATTTCTCCACGCGAAGTAAGATTAAAAGTATTCCTAGATCGCAA
TGGTGACCTAACTGTTCCTAGTTTATATAGTCTTTTCAGAGGAGCTGATTGGCAAGAAAGAGAAACATTCGATATGTATG
GTGTTAACTTTAAAGGTCATCCTCATCCTAAAAGACTTTTAATGCCTGAAGACTGGAAAGGATGGCCTCTCAGAAAAGAC
TATGTTCAGCCTGATTTTTATGAAATGCAAGATGCATACTAA

Upstream 100 bases:

>100_bases
GCTTTGAATCCTTCACTATCTGAAGAGTTTGATCAATCTTTAAAAGAGGTTCAAAAAATATCTGAAGAACTTTCAAGTAA
TTAAGCTTCTCTTATAAAAA

Downstream 100 bases:

>100_bases
TTTATTTACCTTTTTTGTATCTTCTGTAAAACCTCATTATTGCTAAAGCGAGACTTCTTGGGTCGTGCCTCAAAGTTGGA
GTGACCTTTGAACCCTGAAG

Product: NADH dehydrogenase subunit J

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit J; NADH-plastoquinone oxidoreductase subunit J; NDH-1 subunit J; NDH-J

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MTNDSEIVVEEKISGPISDWLSNKGFENIPLKEDHLGIEVIKISPNNLLSIVEALKNDGFNYLQCQGGYDEGPGLNIVCF
YNLIEMNELKEDISPREVRLKVFLDRNGDLTVPSLYSLFRGADWQERETFDMYGVNFKGHPHPKRLLMPEDWKGWPLRKD
YVQPDFYEMQDAY

Sequences:

>Translated_173_residues
MTNDSEIVVEEKISGPISDWLSNKGFENIPLKEDHLGIEVIKISPNNLLSIVEALKNDGFNYLQCQGGYDEGPGLNIVCF
YNLIEMNELKEDISPREVRLKVFLDRNGDLTVPSLYSLFRGADWQERETFDMYGVNFKGHPHPKRLLMPEDWKGWPLRKD
YVQPDFYEMQDAY
>Mature_172_residues
TNDSEIVVEEKISGPISDWLSNKGFENIPLKEDHLGIEVIKISPNNLLSIVEALKNDGFNYLQCQGGYDEGPGLNIVCFY
NLIEMNELKEDISPREVRLKVFLDRNGDLTVPSLYSLFRGADWQERETFDMYGVNFKGHPHPKRLLMPEDWKGWPLRKDY
VQPDFYEMQDAY

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0852

COG function: function code C; NADH:ubiquinone oxidoreductase 27 kD subunit

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 30 kDa subunit family

Homologues:

Organism=Escherichia coli, GI145693162, Length=162, Percent_Identity=34.5679012345679, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NDHJ_PROM1 (A2C0C6)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014201.1
- ProteinModelPortal:   A2C0C6
- SMR:   A2C0C6
- STRING:   A2C0C6
- GeneID:   4779488
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_03721
- eggNOG:   COG0852
- HOGENOM:   HBG727675
- OMA:   YELQDAY
- ProtClustDB:   PRK12494
- BioCyc:   PMAR167555:NATL1_03721-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01357
- InterPro:   IPR010218
- InterPro:   IPR001268
- InterPro:   IPR020396
- ProDom:   PD001581

Pfam domain/function: PF00329 Complex1_30kDa

EC number: 1.6.5.3

Molecular weight: Translated: 20093; Mature: 19962

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00542 COMPLEX1_30K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDSEIVVEEKISGPISDWLSNKGFENIPLKEDHLGIEVIKISPNNLLSIVEALKNDGF
CCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCC
NYLQCQGGYDEGPGLNIVCFYNLIEMNELKEDISPREVRLKVFLDRNGDLTVPSLYSLFR
CEEEECCCCCCCCCCEEEEEEEHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHC
GADWQERETFDMYGVNFKGHPHPKRLLMPEDWKGWPLRKDYVQPDFYEMQDAY
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
TNDSEIVVEEKISGPISDWLSNKGFENIPLKEDHLGIEVIKISPNNLLSIVEALKNDGF
CCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCC
NYLQCQGGYDEGPGLNIVCFYNLIEMNELKEDISPREVRLKVFLDRNGDLTVPSLYSLFR
CEEEECCCCCCCCCCEEEEEEEHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHC
GADWQERETFDMYGVNFKGHPHPKRLLMPEDWKGWPLRKDYVQPDFYEMQDAY
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA