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The map label for this gene is purH

Identifier: 124025057

GI number: 124025057

Start: 318872

End: 320428

Strand: Reverse

Name: purH

Synonym: NATL1_03441

Alternate gene names: 124025057

Gene position: 320428-318872 (Counterclockwise)

Preceding gene: 124025059

Following gene: 124025052

Centisome position: 17.18

GC content: 35.97

Gene sequence:

>1557_bases
ATGTCACCGATAGCTCTGCTAAGTGTCTCAGACAAAACTGGCTTAATTCCACTTGCGAAAGCATTAGTTAATGAACTGGG
CTTCAAAATCATTTCAAGTGGCGGGACTGCAAAGTTAATTGAGAGTGAAAATCTTCCTGTTACAAGAGTCGCAGACTACA
CAGGATTCCCAGAGATTCTTGGAGGAAGAGTAAAAACTCTAAACCCAAAAATTCATGGAGGGATATTAGCCAGACGAGAT
AAACAATCTCATTTAGATGATTTAGATAAACAAAATATCAATCCAATAGACTTGGTGGTTGTTAACTTATATCCATTTGT
AAAAACAATTTCCAAAGAGAATGTTTCATGGGAGGAAGCTATCGAAAATATTGATATTGGCGGTCCAACAATGATCCGAG
CAGCAGCAAAAAACCATCAAGATGTTCTTGTAGTTACTGATCCAAGTCAATACTCAAACTTAATTGATGCCTATAAATCA
AAAAAGATCACTCCTGAATTACGAAAAAAATATTCGCAACAAGCTTTTGAGCATACCGCGACGTATGACCTAACAATAAG
TAATTGGATTGCCAACCAAAGCTCCTCAAAAAAGGTTTCTTGGTTGCAAAGCTTGCCATTAAAGCAAGAACTTAGGTACG
GAGAAAATCCTCATCAAAAAGCTTCATGGTATGGAGAGCCTGAAAAAGGATGGAGTGGAGCTAATCAATTACAAGGCAAA
GAATTAAGTACAAATAATCTTCTAGATCTTGAGGCTGCTTTATCTACTCTTCGTGAATTTGGGTATAAAAATACTATTAG
TAACCCTTCATATCAAAAAGCAGCGGTAGTAATTAAGCATACAAATCCTTGTGGAGTAGCTATTGGAGATTCTCCATCTT
CAGCTCTTAAAAGAGCATTAGATGGCGATAGAGTAAGTGCTTTTGGGGGTATTATTGCTATCAATTGCCCCGTTGATGAA
GCTGCAGCAAAAGAAATTGAAAATATATTTATTGAATGTGTTGTAGCTCCATATTTTGATGAAACTGCAAAAGAAATACT
TTCAAAAAAGAAAAATCTTAGGCTCTTAGAATTAAAAGCTGAGTCTGTCCAAAAAGCAGATAAAAATCACATAAGAAGTA
TACTTGGTGGTTTATTAATTCAAGATTTAGACGAACCAAGTATTGATCAAAAAAAATGGAAAAGTGTTACTGAACTAATC
CCAACAGATGAAGAAATGAATGACTTATCTTTTGCTTGGAAAATTGTAAAACATATACGATCAAACGCAATAGCTGTTGC
ATCCAATCAGCAGAGTCTAGGGATTGGAGCTGGCCAAATGAATAGGGTAGGTTCAGCTAAACTTGCATTAGAAGCTGCTG
GCACAAAATCAAAAGGTGCTGTTTTGGCTAGTGATGGTTTTTTCCCATTCGACGATACTGTAAAGATGGCTTCTGATTAT
GGTATTAGTTCAATTATTCAGCCAGGTGGAAGCATTAGAGACGAAGATTCTATTAAAGCCTGCAATGAATTAGGAATAAA
AATGATTCTTACTGGTAAAAGGCACTTTTTACATTGA

Upstream 100 bases:

>100_bases
CGCGGAATCAGAGTTTAAGGAGATTAGTTCAGTCATCTTGAATGCAATATTCTTCTTTGATGCGTAGGTTATCGAGAAGA
ATCGATTTCAAAAAATAAAA

Downstream 100 bases:

>100_bases
GACAAACTCAAAGAGTTTGATTTCTTAAATATATTATTTCTTACTTTAAATTTATACTTAATTATGATCCTTTTGGATAA
TAAGTTATTTTATTAGACTT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSPIALLSVSDKTGLIPLAKALVNELGFKIISSGGTAKLIESENLPVTRVADYTGFPEILGGRVKTLNPKIHGGILARRD
KQSHLDDLDKQNINPIDLVVVNLYPFVKTISKENVSWEEAIENIDIGGPTMIRAAAKNHQDVLVVTDPSQYSNLIDAYKS
KKITPELRKKYSQQAFEHTATYDLTISNWIANQSSSKKVSWLQSLPLKQELRYGENPHQKASWYGEPEKGWSGANQLQGK
ELSTNNLLDLEAALSTLREFGYKNTISNPSYQKAAVVIKHTNPCGVAIGDSPSSALKRALDGDRVSAFGGIIAINCPVDE
AAAKEIENIFIECVVAPYFDETAKEILSKKKNLRLLELKAESVQKADKNHIRSILGGLLIQDLDEPSIDQKKWKSVTELI
PTDEEMNDLSFAWKIVKHIRSNAIAVASNQQSLGIGAGQMNRVGSAKLALEAAGTKSKGAVLASDGFFPFDDTVKMASDY
GISSIIQPGGSIRDEDSIKACNELGIKMILTGKRHFLH

Sequences:

>Translated_518_residues
MSPIALLSVSDKTGLIPLAKALVNELGFKIISSGGTAKLIESENLPVTRVADYTGFPEILGGRVKTLNPKIHGGILARRD
KQSHLDDLDKQNINPIDLVVVNLYPFVKTISKENVSWEEAIENIDIGGPTMIRAAAKNHQDVLVVTDPSQYSNLIDAYKS
KKITPELRKKYSQQAFEHTATYDLTISNWIANQSSSKKVSWLQSLPLKQELRYGENPHQKASWYGEPEKGWSGANQLQGK
ELSTNNLLDLEAALSTLREFGYKNTISNPSYQKAAVVIKHTNPCGVAIGDSPSSALKRALDGDRVSAFGGIIAINCPVDE
AAAKEIENIFIECVVAPYFDETAKEILSKKKNLRLLELKAESVQKADKNHIRSILGGLLIQDLDEPSIDQKKWKSVTELI
PTDEEMNDLSFAWKIVKHIRSNAIAVASNQQSLGIGAGQMNRVGSAKLALEAAGTKSKGAVLASDGFFPFDDTVKMASDY
GISSIIQPGGSIRDEDSIKACNELGIKMILTGKRHFLH
>Mature_517_residues
SPIALLSVSDKTGLIPLAKALVNELGFKIISSGGTAKLIESENLPVTRVADYTGFPEILGGRVKTLNPKIHGGILARRDK
QSHLDDLDKQNINPIDLVVVNLYPFVKTISKENVSWEEAIENIDIGGPTMIRAAAKNHQDVLVVTDPSQYSNLIDAYKSK
KITPELRKKYSQQAFEHTATYDLTISNWIANQSSSKKVSWLQSLPLKQELRYGENPHQKASWYGEPEKGWSGANQLQGKE
LSTNNLLDLEAALSTLREFGYKNTISNPSYQKAAVVIKHTNPCGVAIGDSPSSALKRALDGDRVSAFGGIIAINCPVDEA
AAKEIENIFIECVVAPYFDETAKEILSKKKNLRLLELKAESVQKADKNHIRSILGGLLIQDLDEPSIDQKKWKSVTELIP
TDEEMNDLSFAWKIVKHIRSNAIAVASNQQSLGIGAGQMNRVGSAKLALEAAGTKSKGAVLASDGFFPFDDTVKMASDYG
ISSIIQPGGSIRDEDSIKACNELGIKMILTGKRHFLH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=479, Percent_Identity=37.7870563674322, Blast_Score=265, Evalue=5e-71,
Organism=Escherichia coli, GI1790439, Length=536, Percent_Identity=45.3358208955224, Blast_Score=440, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71985564, Length=480, Percent_Identity=38.3333333333333, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI71985574, Length=310, Percent_Identity=28.3870967741935, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=64, Percent_Identity=46.875, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323056, Length=472, Percent_Identity=37.7118644067797, Blast_Score=264, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6323768, Length=472, Percent_Identity=37.0762711864407, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24649832, Length=479, Percent_Identity=37.7870563674322, Blast_Score=256, Evalue=4e-68,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_PROM1 (A2C098)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014173.1
- ProteinModelPortal:   A2C098
- SMR:   A2C098
- STRING:   A2C098
- GeneID:   4779059
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_03441
- eggNOG:   COG0138
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- BioCyc:   PMAR167555:NATL1_03441-MONOMER
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 56646; Mature: 56515

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPIALLSVSDKTGLIPLAKALVNELGFKIISSGGTAKLIESENLPVTRVADYTGFPEIL
CCCEEEEEECCCCCCHHHHHHHHHHHCCEEECCCCCEEEEECCCCCEEEECCCCCCHHHH
GGRVKTLNPKIHGGILARRDKQSHLDDLDKQNINPIDLVVVNLYPFVKTISKENVSWEEA
CCCEEECCCCCCCCEEECCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCCCCHHHH
IENIDIGGPTMIRAAAKNHQDVLVVTDPSQYSNLIDAYKSKKITPELRKKYSQQAFEHTA
HHHCCCCCCHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
TYDLTISNWIANQSSSKKVSWLQSLPLKQELRYGENPHQKASWYGEPEKGWSGANQLQGK
EEEEEEHHHHCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCC
ELSTNNLLDLEAALSTLREFGYKNTISNPSYQKAAVVIKHTNPCGVAIGDSPSSALKRAL
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCEEEECCCHHHHHHHHH
DGDRVSAFGGIIAINCPVDEAAAKEIENIFIECVVAPYFDETAKEILSKKKNLRLLELKA
CCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHCCCEEEEECH
ESVQKADKNHIRSILGGLLIQDLDEPSIDQKKWKSVTELIPTDEEMNDLSFAWKIVKHIR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
SNAIAVASNQQSLGIGAGQMNRVGSAKLALEAAGTKSKGAVLASDGFFPFDDTVKMASDY
CCEEEEECCCCCCCCCCCHHCCCCCCEEEEEECCCCCCCEEEECCCCCCCCHHHHHHHCC
GISSIIQPGGSIRDEDSIKACNELGIKMILTGKRHFLH
CHHHHHCCCCCCCCCHHHHHHHHCCEEEEEECCHHCCC
>Mature Secondary Structure 
SPIALLSVSDKTGLIPLAKALVNELGFKIISSGGTAKLIESENLPVTRVADYTGFPEIL
CCEEEEEECCCCCCHHHHHHHHHHHCCEEECCCCCEEEEECCCCCEEEECCCCCCHHHH
GGRVKTLNPKIHGGILARRDKQSHLDDLDKQNINPIDLVVVNLYPFVKTISKENVSWEEA
CCCEEECCCCCCCCEEECCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCCCCHHHH
IENIDIGGPTMIRAAAKNHQDVLVVTDPSQYSNLIDAYKSKKITPELRKKYSQQAFEHTA
HHHCCCCCCHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
TYDLTISNWIANQSSSKKVSWLQSLPLKQELRYGENPHQKASWYGEPEKGWSGANQLQGK
EEEEEEHHHHCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCC
ELSTNNLLDLEAALSTLREFGYKNTISNPSYQKAAVVIKHTNPCGVAIGDSPSSALKRAL
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCEEEECCCHHHHHHHHH
DGDRVSAFGGIIAINCPVDEAAAKEIENIFIECVVAPYFDETAKEILSKKKNLRLLELKA
CCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHCCCEEEEECH
ESVQKADKNHIRSILGGLLIQDLDEPSIDQKKWKSVTELIPTDEEMNDLSFAWKIVKHIR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
SNAIAVASNQQSLGIGAGQMNRVGSAKLALEAAGTKSKGAVLASDGFFPFDDTVKMASDY
CCEEEEECCCCCCCCCCCHHCCCCCCEEEEEECCCCCCCEEEECCCCCCCCHHHHHHHCC
GISSIIQPGGSIRDEDSIKACNELGIKMILTGKRHFLH
CHHHHHCCCCCCCCCHHHHHHHHCCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA