Click here to switch to the map view.

The map label for this gene is proS [H]

Identifier: 124027657

GI number: 124027657

Start: 772754

End: 773896

Strand: Reverse

Name: proS [H]

Synonym: Hbut_0779

Alternate gene names: 124027657

Gene position: 773896-772754 (Counterclockwise)

Preceding gene: 124027658

Following gene: 124027653

Centisome position: 46.42

GC content: 55.21

Gene sequence:

>1143_bases
TTGCCGGGTGCTAGCCGGGACCGGTGGAGGAGAGAGTTCCCCCGCTGGTTCGACTGGATTCTCGAGGAGGCAGGCTTCTA
CGACTATGGACGCTACCCCGTCAAGGGCATGGGTATATGGCTGCCCTACGGGTTCCAGCTGCGCCGTAGAATCATAGAAC
TAGTAAGGGAGGTTCTGGACTCTACGGGGCACGAGGAAATACTGATGCCTCTACTGATCCCAGAGCACCTCTTGAGGAAG
GAGAGCGAGCACATACGCGGCTTCGAGGGTGAGGTCTACTGGGTAACCCATGGCGGGCACGAGCCCCTTGACGTGAAGCT
TGCGCTGCGTCCAACAAGCGAGACAAGCATAACCTACATGGAGAGCTTCTGGATTAAGAGCTATCGTCAGCTCCCAAAGA
AGTACTACCAGATAGTCAGCATATTCCGCTACGAGACGAAGGCTACGAGGCCGATGATAAGGCTGCGTGAGGTAACCACG
TTCAAGGAGGCCCACACGGTGCACGATAGTTTCGCTGACGCGGACCGCCAGGTTGCCGAGGCTATAGAGCTCTACAAGAA
GATATTCGACGAGCTAGGCATACCCTATGTGATTAGCCGGAGGCCGGAATGGGACAAGTTCGCCGGTGCAGTCTATACCG
TGGCCTTTGACACCATTATGCCGGATGGTAAGACGCTGCAGATAGGCACGGTTCACCACCTGGGCCAGAACTTCACGGTA
CCCTTTGACGTCAAGATACAGTTGCGCGACGAAAGTATCGACTATGCGTGGCAGACAAGCTACGGTATAAGTGACCGCAT
AGTAGCCACGGTAATAGCAATACATGGCGATGATCGTGGTGCTGTGTTGCCGCCAAACGTGGCTCCCATACAGGTAGTGG
TTATACCAATACCAGCTGGAGATGACGAGGAAAAGAAGAGGCTGGTAGCTGAGCATCTCGAGAAGGTTAAGGAGGTGTTG
AAGAAGGCTGGGATAAGGTTCCGCGTGGATGACCGTGAGGATGTGAGGCCAGGTAGGAAGTTCTACGAGTGGGAGGCCAA
GGGCGTACCAGTACGCATCGAGGTTGGGCTACGTGAAGCCGAGACTGGCACGGTTCTCGTAGCCCGTAGGGACACCCTAG
AGAAGACCGTGGTTAAGCTTTGA

Upstream 100 bases:

>100_bases
CTAGGTCAGACAAGCATTTTTACACACTGGGAGCCCAGCCTTTATAGCCCACCTATGTTATCCCTTTAGCCACCGAGGCT
TTTGCGAGGTACCACAGCCC

Downstream 100 bases:

>100_bases
AGAGCTACCAGACTTCCGGGCAAAGCTGTTCGAGGACATAAGGAGAAACCTACGCGAGAAAGCTTGGCAGTGGCTAAGTA
CGATGGTTAAGCGTGTCGAG

Product: bifunctional aminoacyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MPGASRDRWRREFPRWFDWILEEAGFYDYGRYPVKGMGIWLPYGFQLRRRIIELVREVLDSTGHEEILMPLLIPEHLLRK
ESEHIRGFEGEVYWVTHGGHEPLDVKLALRPTSETSITYMESFWIKSYRQLPKKYYQIVSIFRYETKATRPMIRLREVTT
FKEAHTVHDSFADADRQVAEAIELYKKIFDELGIPYVISRRPEWDKFAGAVYTVAFDTIMPDGKTLQIGTVHHLGQNFTV
PFDVKIQLRDESIDYAWQTSYGISDRIVATVIAIHGDDRGAVLPPNVAPIQVVVIPIPAGDDEEKKRLVAEHLEKVKEVL
KKAGIRFRVDDREDVRPGRKFYEWEAKGVPVRIEVGLREAETGTVLVARRDTLEKTVVKL

Sequences:

>Translated_380_residues
MPGASRDRWRREFPRWFDWILEEAGFYDYGRYPVKGMGIWLPYGFQLRRRIIELVREVLDSTGHEEILMPLLIPEHLLRK
ESEHIRGFEGEVYWVTHGGHEPLDVKLALRPTSETSITYMESFWIKSYRQLPKKYYQIVSIFRYETKATRPMIRLREVTT
FKEAHTVHDSFADADRQVAEAIELYKKIFDELGIPYVISRRPEWDKFAGAVYTVAFDTIMPDGKTLQIGTVHHLGQNFTV
PFDVKIQLRDESIDYAWQTSYGISDRIVATVIAIHGDDRGAVLPPNVAPIQVVVIPIPAGDDEEKKRLVAEHLEKVKEVL
KKAGIRFRVDDREDVRPGRKFYEWEAKGVPVRIEVGLREAETGTVLVARRDTLEKTVVKL
>Mature_379_residues
PGASRDRWRREFPRWFDWILEEAGFYDYGRYPVKGMGIWLPYGFQLRRRIIELVREVLDSTGHEEILMPLLIPEHLLRKE
SEHIRGFEGEVYWVTHGGHEPLDVKLALRPTSETSITYMESFWIKSYRQLPKKYYQIVSIFRYETKATRPMIRLREVTTF
KEAHTVHDSFADADRQVAEAIELYKKIFDELGIPYVISRRPEWDKFAGAVYTVAFDTIMPDGKTLQIGTVHHLGQNFTVP
FDVKIQLRDESIDYAWQTSYGISDRIVATVIAIHGDDRGAVLPPNVAPIQVVVIPIPAGDDEEKKRLVAEHLEKVKEVLK
KAGIRFRVDDREDVRPGRKFYEWEAKGVPVRIEVGLREAETGTVLVARRDTLEKTVVKL

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=372, Percent_Identity=34.9462365591398, Blast_Score=223, Evalue=2e-58,
Organism=Escherichia coli, GI1786392, Length=166, Percent_Identity=29.5180722891566, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71991342, Length=373, Percent_Identity=34.3163538873995, Blast_Score=225, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI71991346, Length=349, Percent_Identity=35.243553008596, Blast_Score=223, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6321808, Length=376, Percent_Identity=35.6382978723404, Blast_Score=240, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24649466, Length=375, Percent_Identity=34.1333333333333, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24649468, Length=376, Percent_Identity=34.0425531914894, Blast_Score=214, Evalue=6e-56,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 44152; Mature: 44021

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGASRDRWRREFPRWFDWILEEAGFYDYGRYPVKGMGIWLPYGFQLRRRIIELVREVLD
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
STGHEEILMPLLIPEHLLRKESEHIRGFEGEVYWVTHGGHEPLDVKLALRPTSETSITYM
CCCCHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEECCCCCCEEEEEEEECCCCCHHHHH
ESFWIKSYRQLPKKYYQIVSIFRYETKATRPMIRLREVTTFKEAHTVHDSFADADRQVAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIELYKKIFDELGIPYVISRRPEWDKFAGAVYTVAFDTIMPDGKTLQIGTVHHLGQNFTV
HHHHHHHHHHHCCCCEEECCCCCHHHHCCEEEEEEEEHCCCCCCEEEEECHHHCCCCCCC
PFDVKIQLRDESIDYAWQTSYGISDRIVATVIAIHGDDRGAVLPPNVAPIQVVVIPIPAG
EEEEEEEEECCCCCEEEECCCCCCHHEEEEEEEEECCCCCCCCCCCCCCEEEEEEEECCC
DDEEKKRLVAEHLEKVKEVLKKAGIRFRVDDREDVRPGRKFYEWEAKGVPVRIEVGLREA
CCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEECCCCCEEEEECCEEC
ETGTVLVARRDTLEKTVVKL
CCCEEEEEECHHHHHHHHCC
>Mature Secondary Structure 
PGASRDRWRREFPRWFDWILEEAGFYDYGRYPVKGMGIWLPYGFQLRRRIIELVREVLD
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
STGHEEILMPLLIPEHLLRKESEHIRGFEGEVYWVTHGGHEPLDVKLALRPTSETSITYM
CCCCHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEECCCCCCEEEEEEEECCCCCHHHHH
ESFWIKSYRQLPKKYYQIVSIFRYETKATRPMIRLREVTTFKEAHTVHDSFADADRQVAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIELYKKIFDELGIPYVISRRPEWDKFAGAVYTVAFDTIMPDGKTLQIGTVHHLGQNFTV
HHHHHHHHHHHCCCCEEECCCCCHHHHCCEEEEEEEEHCCCCCCEEEEECHHHCCCCCCC
PFDVKIQLRDESIDYAWQTSYGISDRIVATVIAIHGDDRGAVLPPNVAPIQVVVIPIPAG
EEEEEEEEECCCCCEEEECCCCCCHHEEEEEEEEECCCCCCCCCCCCCCEEEEEEEECCC
DDEEKKRLVAEHLEKVKEVLKKAGIRFRVDDREDVRPGRKFYEWEAKGVPVRIEVGLREA
CCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEECCCCCEEEEECCEEC
ETGTVLVARRDTLEKTVVKL
CCCEEEEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]