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The map label for this gene is hypF [H]

Identifier: 124027632

GI number: 124027632

Start: 742437

End: 744752

Strand: Reverse

Name: hypF [H]

Synonym: Hbut_0753

Alternate gene names: 124027632

Gene position: 744752-742437 (Counterclockwise)

Preceding gene: 124027633

Following gene: 124027626

Centisome position: 44.67

GC content: 58.33

Gene sequence:

>2316_bases
ATGGCTAACCGTGAAGCACTACGGGTACGTGTTGTAGGCCTCGTGCAGGGTGTAGGCTTTCGACCGTTTGTACACCGGCT
AGCTGAGAGGCTAGGCCTCGCCGGCTACGTCCGCAACATGGGCGGCTCTGAGGTAGAAATACTTGTTGAGGGGCCGCCAG
ACAGCCTTAGATTGTTTGTGAGCCTGTTGGAGAAGGAGAAGCCACCACCAGCCATCATAGAGGAGATGCGTGTCGAGCCG
GTACCAGCTCGTGGCCTCACGGTGTTTCGTATAGAGAAGAGTAGCCGTAGCAGAAAGGAGCGAAGCATGATACCGCCGGA
CTTCGGCATCTGCGACTGGTGCGCCCGGGAGGTCCTGGAGCCTGGTACAAGGTTCTACAGGTACCCTTGGACCAGCTGTG
CCTGGTGTGGGCCAAGATTCTCGATGATGTACGACATACCCTATGACCGTGAAAACACTGCTATGAGAGAATTTCCCTTC
TGTGAGGAGTGCCGCCGCGACTACGAGGACATGGGTAATATTAGGAGGTACCATGCGCAGGGTTTCAGCTGCCCCCGCTG
CGGCCCCAAAACCATCCTCTTGGACGGTCGTGGCGAGCCTCTAGACGTTGAAGACCCGGTTGCGGAGGCTGCAAGGCTGA
TAGATGAGGGCCATATTGTCGCTGTCAAGGGGGTTGGTGGCTTCCACATAGCATCCCTTGCAACAGACGACGAGGTCGTG
GCCCGGGTTAGGAGGATCAAGTCCAGGCCCACCCAGCCCCTAGCGCTTATGGCTAGGGATTGTAGTGTGGCTGCTGGGCT
GGTGGCGCTTGACGCCCATGCATGCCAGCTCCTCCGAAGCCCCCGCCGCCCAATACTCCTACTCCCAAAACGTGATGATG
CCCCGGTATCCCCGCTTGTGGCCCCCGGCCTGGACACCCTGGGCATCATGCTGCCGTACACTGGGCTCCACCTCCTACTC
CTCCACATGGTGCGGGACGGATTCCTAGTAATGACTAGCGGGAACCGCCACGGAATGCCGATGTGCAGGACCGTGGAGTG
TATCCTGGACCAGTTGGGCGGCGAAATAGACTACATACTCACGCATAACAGGGAGATAGTACACCGTGTCGACGACAGTG
TTGTACGCTTCACGGCTGGCAAGCCTGTACTCCTAAGGAGGGCTAGGGGCTACGCGCCCGAATGGATACGTATCAGCCGC
AGCGTCGGGGAGCACGTAGCGGTTGGAGCCGACCTGCAAACAGCTGGGGCAGTAAGCTTTGACGATAAGGTCGTGCTAAC
ACAATACATCGGAGACCTCGACGAGCCTGGCCAGCTCGAGGAGCTGGAGGAGGAGCTAAAGTGGCTCATAAGAGTCTATG
GGCTGAGGCCTAGTAGCGTTGCTCTCGACATGCACCCAGCCTATGAGAGTAGACGGCTAGCTGCAAAGCTCTCAGAAGAG
CTAGATGCCGAGCCCGTCGAGGTTCAGCATCATCACGCCCACGCCGTAGCTGCAGCTGCTGATGCTGGCTTGGATCCATG
GGACTACGTCCCGGCGGTAACAGTTGATGGTGCAGGCTACGGCGTGGATGGCGTTATATGGGGTGGCGAGGCCATGATCG
CTGGTGCTGAAGGGTTTGAGAGAACTGGTACCATAAAGCCGTTCCCCTTGCCGGGCGGCGATACAGCCACCAAGAGGCCC
CTAAGAATACTCATAGGCATACTATCCCAGGGCTGGGGCCTGGACGAGATACTACAGCTACTCGAGAAGATGGGCCACCT
TGGCAAGGGGATAAGTGAGGAGGAAGCAGTAACCTCCTACAAGCTTGCCAGGAGCAACCGTACACCACTAGCAAGCAGCG
CTGGCAGACTCCTCGACGCGGTATCAGCACTCCTAGGCCTAAGGTATAGGAGGACCTACGAGGGTGAACCAGCGATAGTA
CTAGAGGCCTATGCTAGACGCCACGGCGGCAAACCACTACCCGACGAGCCGCCAGCAACAAGCTACAAGGGCCTCAGCGC
TCTCGACCCTCTACGGGTCTTGGAATGGGTTGTGGAGAAGCTGGAAGAGGGTGTGGAGCCCTGGCACCTAGCCGCCACGG
TGATGTACTGGGTTGGCCGCCACCTGGCCGAGACAGTGCTAGAAAACTATGACGGGGCTCGCGATAAACCACTCCTCATC
TCTGGCGGTGCAGCAGTAAACGACTACATCTACATGGGTGCGGCAGACGCCGCCAAGGCGTATGGTAGAACAGTACACAT
ACCTGGCAAGGTGCCGCCAGGCGACGGCGGAATAGCCCTAGGCCAAATAATAGTGGCAGCAGCCAAAACAAGGTGA

Upstream 100 bases:

>100_bases
GGATGAGCTTGAGAAACAGGTTAGAGAAATAGAGGAGCTTCTCCGGAGCCTGGAGGAGAAGGCCGAGGAGATAGAGAAAA
TGGTTTGGGGCAAGTAGCGT

Downstream 100 bases:

>100_bases
CAAGCCCGGGACTCTAGCCAGACTATTTACCACAATCCCCGTTCCAAGGCCTCCCCGGAGCATATGAGCGCTCCCCGCCC
GTCCGGGGACTGCCATGTTT

Product: hydrogenase maturation protein

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 771; Mature: 770

Protein sequence:

>771_residues
MANREALRVRVVGLVQGVGFRPFVHRLAERLGLAGYVRNMGGSEVEILVEGPPDSLRLFVSLLEKEKPPPAIIEEMRVEP
VPARGLTVFRIEKSSRSRKERSMIPPDFGICDWCAREVLEPGTRFYRYPWTSCAWCGPRFSMMYDIPYDRENTAMREFPF
CEECRRDYEDMGNIRRYHAQGFSCPRCGPKTILLDGRGEPLDVEDPVAEAARLIDEGHIVAVKGVGGFHIASLATDDEVV
ARVRRIKSRPTQPLALMARDCSVAAGLVALDAHACQLLRSPRRPILLLPKRDDAPVSPLVAPGLDTLGIMLPYTGLHLLL
LHMVRDGFLVMTSGNRHGMPMCRTVECILDQLGGEIDYILTHNREIVHRVDDSVVRFTAGKPVLLRRARGYAPEWIRISR
SVGEHVAVGADLQTAGAVSFDDKVVLTQYIGDLDEPGQLEELEEELKWLIRVYGLRPSSVALDMHPAYESRRLAAKLSEE
LDAEPVEVQHHHAHAVAAAADAGLDPWDYVPAVTVDGAGYGVDGVIWGGEAMIAGAEGFERTGTIKPFPLPGGDTATKRP
LRILIGILSQGWGLDEILQLLEKMGHLGKGISEEEAVTSYKLARSNRTPLASSAGRLLDAVSALLGLRYRRTYEGEPAIV
LEAYARRHGGKPLPDEPPATSYKGLSALDPLRVLEWVVEKLEEGVEPWHLAATVMYWVGRHLAETVLENYDGARDKPLLI
SGGAAVNDYIYMGAADAAKAYGRTVHIPGKVPPGDGGIALGQIIVAAAKTR

Sequences:

>Translated_771_residues
MANREALRVRVVGLVQGVGFRPFVHRLAERLGLAGYVRNMGGSEVEILVEGPPDSLRLFVSLLEKEKPPPAIIEEMRVEP
VPARGLTVFRIEKSSRSRKERSMIPPDFGICDWCAREVLEPGTRFYRYPWTSCAWCGPRFSMMYDIPYDRENTAMREFPF
CEECRRDYEDMGNIRRYHAQGFSCPRCGPKTILLDGRGEPLDVEDPVAEAARLIDEGHIVAVKGVGGFHIASLATDDEVV
ARVRRIKSRPTQPLALMARDCSVAAGLVALDAHACQLLRSPRRPILLLPKRDDAPVSPLVAPGLDTLGIMLPYTGLHLLL
LHMVRDGFLVMTSGNRHGMPMCRTVECILDQLGGEIDYILTHNREIVHRVDDSVVRFTAGKPVLLRRARGYAPEWIRISR
SVGEHVAVGADLQTAGAVSFDDKVVLTQYIGDLDEPGQLEELEEELKWLIRVYGLRPSSVALDMHPAYESRRLAAKLSEE
LDAEPVEVQHHHAHAVAAAADAGLDPWDYVPAVTVDGAGYGVDGVIWGGEAMIAGAEGFERTGTIKPFPLPGGDTATKRP
LRILIGILSQGWGLDEILQLLEKMGHLGKGISEEEAVTSYKLARSNRTPLASSAGRLLDAVSALLGLRYRRTYEGEPAIV
LEAYARRHGGKPLPDEPPATSYKGLSALDPLRVLEWVVEKLEEGVEPWHLAATVMYWVGRHLAETVLENYDGARDKPLLI
SGGAAVNDYIYMGAADAAKAYGRTVHIPGKVPPGDGGIALGQIIVAAAKTR
>Mature_770_residues
ANREALRVRVVGLVQGVGFRPFVHRLAERLGLAGYVRNMGGSEVEILVEGPPDSLRLFVSLLEKEKPPPAIIEEMRVEPV
PARGLTVFRIEKSSRSRKERSMIPPDFGICDWCAREVLEPGTRFYRYPWTSCAWCGPRFSMMYDIPYDRENTAMREFPFC
EECRRDYEDMGNIRRYHAQGFSCPRCGPKTILLDGRGEPLDVEDPVAEAARLIDEGHIVAVKGVGGFHIASLATDDEVVA
RVRRIKSRPTQPLALMARDCSVAAGLVALDAHACQLLRSPRRPILLLPKRDDAPVSPLVAPGLDTLGIMLPYTGLHLLLL
HMVRDGFLVMTSGNRHGMPMCRTVECILDQLGGEIDYILTHNREIVHRVDDSVVRFTAGKPVLLRRARGYAPEWIRISRS
VGEHVAVGADLQTAGAVSFDDKVVLTQYIGDLDEPGQLEELEEELKWLIRVYGLRPSSVALDMHPAYESRRLAAKLSEEL
DAEPVEVQHHHAHAVAAAADAGLDPWDYVPAVTVDGAGYGVDGVIWGGEAMIAGAEGFERTGTIKPFPLPGGDTATKRPL
RILIGILSQGWGLDEILQLLEKMGHLGKGISEEEAVTSYKLARSNRTPLASSAGRLLDAVSALLGLRYRRTYEGEPAIVL
EAYARRHGGKPLPDEPPATSYKGLSALDPLRVLEWVVEKLEEGVEPWHLAATVMYWVGRHLAETVLENYDGARDKPLLIS
GGAAVNDYIYMGAADAAKAYGRTVHIPGKVPPGDGGIALGQIIVAAAKTR

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=771, Percent_Identity=35.538261997406, Blast_Score=330, Evalue=2e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 84684; Mature: 84553

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00626 RCC1_2 ; PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANREALRVRVVGLVQGVGFRPFVHRLAERLGLAGYVRNMGGSEVEILVEGPPDSLRLFV
CCCCHHHEEEEEHHHHCCCCCHHHHHHHHHHCHHHHHHCCCCCEEEEEEECCCHHHHHHH
SLLEKEKPPPAIIEEMRVEPVPARGLTVFRIEKSSRSRKERSMIPPDFGICDWCAREVLE
HHHHCCCCCHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHC
PGTRFYRYPWTSCAWCGPRFSMMYDIPYDRENTAMREFPFCEECRRDYEDMGNIRRYHAQ
CCCCEEECCCCCCCCCCCCCEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHC
GFSCPRCGPKTILLDGRGEPLDVEDPVAEAARLIDEGHIVAVKGVGGFHIASLATDDEVV
CCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCEEEEECCCCHHHH
ARVRRIKSRPTQPLALMARDCSVAAGLVALDAHACQLLRSPRRPILLLPKRDDAPVSPLV
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
APGLDTLGIMLPYTGLHLLLLHMVRDGFLVMTSGNRHGMPMCRTVECILDQLGGEIDYIL
CCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEE
THNREIVHRVDDSVVRFTAGKPVLLRRARGYAPEWIRISRSVGEHVAVGADLQTAGAVSF
ECCHHHHHHHCCCEEEEECCCHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCC
DDKVVLTQYIGDLDEPGQLEELEEELKWLIRVYGLRPSSVALDMHPAYESRRLAAKLSEE
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHH
LDAEPVEVQHHHAHAVAAAADAGLDPWDYVPAVTVDGAGYGVDGVIWGGEAMIAGAEGFE
CCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCEEEECCCEEEECCCCCC
RTGTIKPFPLPGGDTATKRPLRILIGILSQGWGLDEILQLLEKMGHLGKGISEEEAVTSY
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
KLARSNRTPLASSAGRLLDAVSALLGLRYRRTYEGEPAIVLEAYARRHGGKPLPDEPPAT
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHCCCCCCCCCCCCC
SYKGLSALDPLRVLEWVVEKLEEGVEPWHLAATVMYWVGRHLAETVLENYDGARDKPLLI
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
SGGAAVNDYIYMGAADAAKAYGRTVHIPGKVPPGDGGIALGQIIVAAAKTR
ECCCEECCEEEECCHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANREALRVRVVGLVQGVGFRPFVHRLAERLGLAGYVRNMGGSEVEILVEGPPDSLRLFV
CCCHHHEEEEEHHHHCCCCCHHHHHHHHHHCHHHHHHCCCCCEEEEEEECCCHHHHHHH
SLLEKEKPPPAIIEEMRVEPVPARGLTVFRIEKSSRSRKERSMIPPDFGICDWCAREVLE
HHHHCCCCCHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHC
PGTRFYRYPWTSCAWCGPRFSMMYDIPYDRENTAMREFPFCEECRRDYEDMGNIRRYHAQ
CCCCEEECCCCCCCCCCCCCEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHC
GFSCPRCGPKTILLDGRGEPLDVEDPVAEAARLIDEGHIVAVKGVGGFHIASLATDDEVV
CCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCEEEEECCCCHHHH
ARVRRIKSRPTQPLALMARDCSVAAGLVALDAHACQLLRSPRRPILLLPKRDDAPVSPLV
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
APGLDTLGIMLPYTGLHLLLLHMVRDGFLVMTSGNRHGMPMCRTVECILDQLGGEIDYIL
CCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEE
THNREIVHRVDDSVVRFTAGKPVLLRRARGYAPEWIRISRSVGEHVAVGADLQTAGAVSF
ECCHHHHHHHCCCEEEEECCCHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCC
DDKVVLTQYIGDLDEPGQLEELEEELKWLIRVYGLRPSSVALDMHPAYESRRLAAKLSEE
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHH
LDAEPVEVQHHHAHAVAAAADAGLDPWDYVPAVTVDGAGYGVDGVIWGGEAMIAGAEGFE
CCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCEEEECCCEEEECCCCCC
RTGTIKPFPLPGGDTATKRPLRILIGILSQGWGLDEILQLLEKMGHLGKGISEEEAVTSY
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
KLARSNRTPLASSAGRLLDAVSALLGLRYRRTYEGEPAIVLEAYARRHGGKPLPDEPPAT
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHCCCCCCCCCCCCC
SYKGLSALDPLRVLEWVVEKLEEGVEPWHLAATVMYWVGRHLAETVLENYDGARDKPLLI
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
SGGAAVNDYIYMGAADAAKAYGRTVHIPGKVPPGDGGIALGQIIVAAAKTR
ECCCEECCEEEECCHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]